Validation of an Easy Acetonitrile Fractionation for the Simplification of Protein Samples Prior to Proteomics Analyses

Yannis Karamanos^*, Barbara Deracinois, Gwënaël Pottiez, Sophie Duban-Deweer

Laboratoire de la Barrière Hémato-encéphalique, Lens, France.

DOI: 10.4236/oalib.1101928                

Abstract

In most proteomics analyses and in particular for the “off-gel” approaches, based essentially on chromatography, the complexity of the proteome should be reduced; otherwise identifications can be hindered, especially if the mass spectrometry analysis is not conducted using state-of-the-art instrumentation. Even if the method used is a bottom-up proteomics, it appears mandatory to pre-fractionate the proteins in order to reduce the complexity. We report here the development and validation of a pre-fractionation based on the differential solubilisation of proteins using increasing concentrations of acetonitrile (ACN). This “ACN fractionation” was applied to the study of the Triton X-100 soluble sub-proteome of brain capillary endothelial cells (BCEC) with re-induced blood-brain barrier (BBB) functions.

Keywords

Proteomics, Acetonitrile, Fractionation

Conflicts of Interest

The authors declare no conflicts of interest.

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