Home > Journals > Biomedical & Life Sciences | Business & Economics > OALibJ
Share This Article:

Effect of Morphological and Physiological Development on the Acclimatization of in Vitro Plants of Bambusa vulgaris Schrad ex Wendl in Liquid Culture Medium

DOI: 10.4236/oalib.1101787    541 Downloads   869 Views   Citations

ABSTRACT

Bambusa vulgaris Schrad. ex Wendl (B. vulgaris) is a species of great interest because it produces the most important non-timber forest products of worldwide. It has become an integral component in social forestry programs. This study aimed to determine the effect of morphological and physiological development on the acclimatization of in vitro plants of B. vulgaris. Morphological and physiological parameters of plants subjected to in vitro conditions were evaluated. Our results showed that the number shoots per explant of B. vulgaris increased when 6.0 μM of 6-benzylade-nine (BA) was incorporated into the culture medium. After six subcultures, clumps of 3 - 6 axes were obtained, and their division in groups of three axes allowed multiplication of the plants. During the acclimatization phase to ex vitro conditions, the in vitro plants showed 75% survival. Results showed that greater development of the root system, height and number of leaves per plants was observed with height larger of 3.0 cm. The leaf area, water content, fresh and dry weight increased with height larger of 3.0 cm, but all parameters were decreased with height (1.0 - 3.0 cm). The correlation between net assimilation rate and leaf area index was significantly positive in vitro plants with height larger of 3.0 cm. The increase of plants of B. vulgaris in liquid culture medium offers new prospects for increasing the commercial production of this species, and for studies that could elucidate morphological and physiological responses in plants of bamboo.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

García-Ramírez, Y. , González-González, M. , Freire-Seijo, M. , O-Cárdenas, M. , León-Quintana, M. , Roque-Morales, B. , Mena-Méndez, E. , Mendoza-Rodríguez, M. and Rivero-Quintana, L. (2015) Effect of Morphological and Physiological Development on the Acclimatization of in Vitro Plants of Bambusa vulgaris Schrad ex Wendl in Liquid Culture Medium. Open Access Library Journal, 2, 1-7. doi: 10.4236/oalib.1101787.

References

[1] Tripathi, Y. and Khawlhring, L. (2010) Bamboo Resource and Its Role in Ecological Security. Indian Forester, 136, 641.
[2] Liu, Y., Zambrano, Y., Hu, C.J., Carmona, E.R., Bernal, A., Pérez, A., et al. (2009) Sugarcane Metabolites Produced in CO2-Rich Temporary Immersion Bioreactors (TIBs) Induce Tomato (Solanum lycopersicum) Resistance against Bacterial Wilt (Ralstonia solanacearum). In Vitro Cellular & Developmental Biology—Plant, 46, 558-568.
[3] Ramanayake, S., Meemaduma, V. and Weerawardene, T. (2006) In Vitro Shoot Proliferation and Enhancement of Rooting for the Large-Scale Propagation of Yellow Bamboo (Bambusa vulgaris “Striata”). Scientia Horticulturae, 110, 109-113.
http://dx.doi.org/10.1016/j.scienta.2006.06.016
[4] Ramanayake, S., Maddegoda, K., Vitharana, M. and Chaturani, G. (2008) Root Induction in Three Species of Bamboo with Different Rooting Abilities. Scientia Horticulturae, 118, 270-273.
http://dx.doi.org/10.1016/j.scienta.2008.06.004
[5] Iarema, L., da Cruz, A.C., Saldanha, C.W., Dias, L.L.C., Vieira, R.F., de liveira, E.J. and Otoni, W.C. (2012) Photoautotrophic Propagation of Brazilian Ginseng [Pfaffia glomerata (Spreng.) Pedersen]. Plant Cell, Tissue and Organ Culture (PCTOC), 110, 227-238.
http://dx.doi.org/10.1007/s11240-012-0145-6
[6] Aitken-Christie, J., Kozai, T. and Takayama, S. (1995) Automation in Plant Tissue Culture—General Introduction and Overview. In: Aitken-Christie, J., Kozai, T. and Smith, L., Eds., Automation and Environmental Control in Plant Tissue Culture, Kluwer Academic Publishers, Dordrecht, 1-18.
[7] Mudoi, K.D., Saikia, S.P., Goswami, A., Gogoi, A., Bora, D. and Borthakur, M. (2013) Micropropagation of Important Bamboos: A Review. African Journal of Biotechnology, 12, 2770-2785.
[8] Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Culture. Physiologia Plantarum, 15, 473-497.
http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
[9] Bisht, P., Pant, M. and Kant, A. (2010) In Vitro Propagation of Gigantochloa atroviolaceae Widjaja through Nodal Explants. Journal of American Science, 6, 1019-1026.
[10] Pratibha, S. and Sarma, K.P. (2013) In Vitro Propagation of Bambusa tulda: An Important Plant for Better Environment. Journal of Environmental Research and Development, 7, 1216-1223.
[11] Jiménez, V., Castillo, J., Tavares, E., Guevara, E. and Montiel, M. (2006) In Vitro Propagation of the Neotropical Giant Bamboo, Guadua angustifolia Kunth, through Axillary Shoot Proliferation. Plant Cell, Tissue and Organ Culture, 86, 389-395.
http://dx.doi.org/10.1007/s11240-006-9120-4
[12] Ndiaye, A., Mamadou, S., Niang, D. and Gassama-Dia, Y. (2006) In Vitro Regeneration of Adult Trees of Bambusa vulgaris. African Journal of Biotechnology, 5, 1245-1248.
[13] Hazarika, B.N. (2006) Morpho-Physiological Disorders in in Vitro Culture of Plants. Scientia Horticulturae, 108, 105-120.
http://dx.doi.org/10.1016/j.scienta.2006.01.038
[14] Sayanika, W. and Sharma, G. (2009) In Vitro Propagation of Arundinaria callosa Munro—An Edible Bamboo from Nodal Explants of Mature Plants. The Open Plant Science Journal, 3, 35-39.
http://dx.doi.org/10.2174/1874294700903010035
[15] Kozai, T. (2010) Photoautotrophic Micropropagation–Environmental Control for Promoting Photosynthesis. Propagation of Ornamental Plants, 10, 188-204.
[16] Aragón, C., Carvalho, L., González, J., Escalona, M. and Amancio, S. (2010) Ex Vitro Acclimatization of Plantain Plantlets Micropropagated in Temporary Immersion Bioreactor. Biologia Plantarum, 54, 237-244.
http://dx.doi.org/10.1007/s10535-010-0042-y
[17] Cha-um, S., Puthea, O. and Kirdmanee, C. (2009) An Effective In-Vitro Acclimatization Using Uniconazole Treatments and Ex-Vitro Adaptation of Phalaenopsis orchid. Scientia Horticulturae, 121, 468-473.
http://dx.doi.org/10.1016/j.scienta.2009.02.027
[18] Quiala, E., Canñ；al, M., Meijón, M., Rodriguez, R., Chávez, M., Valledor, L., de Feria, M. and Barbón, R. (2012) Morphological and Physiological Responses of Proliferating Shoots of Teak to Temporary Immersion and BA Treatments. Plant Cell, Tissue and Organ Culture (PCTOC), 109, 223-234.
http://dx.doi.org/10.1007/s11240-011-0088-3
[19] Hurtado, O., Freire-Seijo, M., Leiva-Mora, M. and García-Ramírez, Y. (2011) Influencia de las características de las plantas cultivadas in Vitro de Bambusa vulgaris var. vulgaris Schrad. ex Wendl. en su aclimatización. Biotecnología Vegetal, 11, 155-161.
[20] Haworth, M., Elliott-Kingston, C. and McElwain, J.C. (2013) Co-Ordination of Physiological and Morphological Responses of Stomata to Elevated [CO2] in Vascular Plants. Oecologia, 171, 71-82.
http://dx.doi.org/10.1007/s00442-012-2406-9

  
comments powered by Disqus
OALibJ Subscription
E-Mail Alert
OALibJ Most popular papers
Publication Ethics & OA Statement
OALibJ News
Frequently Asked Questions
Recommend to Peers
Recommend to Library
Contact Us

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.