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Cu(II) Benzoylpyridine Thiosemicarbazone Complexes: Inhibition of Human Topoisomerase IIα and Activity against Breast Cancer Cells

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DOI: 10.4236/ojic.2016.62010    2,649 Downloads   3,767 Views   Citations

ABSTRACT

The focus of this research is on the study of a series of copper (II) benzoylpyridine thiosemicarbazone complexes. Of the six benzoylpyridine thiosemicarbazone ligands used in this study, two are reported for the first time; 2-benzoylpyridine tert-butyl thiosemicarbazone (BZP-tBTSC), and 2-benzoylpyridine benzyl thiosemicarbazone (BZP-BzTSC). Once characterized by NMR, melting point, and MS, these mono-anionic tridentate ligands were then reacted with Cu2+ to form the new square planar metal complexes [Cu(BZP-tBTSC)Cl] and [Cu(BZP-BzTSC)Cl]. All of the copper complexes display marked inhibition of human topoisomerase IIα. The [Cu(BZP-tBTSC)Cl] complex shows marked activity against human breast cancer cell lines.

Received 15 March 2016; accepted 26 April 2016; published 29 April 2016

1. Introduction

Thiosemicarbazones are an interesting class of chemical compounds that have active biological and medicinal qualities and have been used as drugs against leukemia [1] [2] and help cure leprosy, tuberculosis, and smallpox [3] - [5] . Additionally, these compounds also act as ligands for a wide variety of transition metals, often as high affinity multi-dentate chelating agents [1] [6] [7] .

A specific subset of thiosemicarbazone ligands that have been referred to as α-(N)-heterocyclic thiosemicarbazones have potent antiproliferative properties against a wide variety of microbes and cancer cells. Specifically, α-(N)-heterocyclic thiosemicarbazones have been shown to have activity against ribonucleotide reductase (RNR) [8] - [10] . Further, Triapine®, or 3-aminopyridine-2-carboxaldehyde thiosemicarbazone, recently entered Stage II clinical trials as an anticancer agent [11] - [13] . In addition, α-N-heterocyclic thiosemicarbazones, with their N-N-S tridentate coordination backbone, are particularly well-known for their ability to chelate to biologically important transition metals, such as Cu(II) and Fe(II) [14] - [18] .

In a fortuitous coincidence, the copper complexes of α-(N)-heterocyclic thiosemicarbazones appear to have high antineoplastic activity through an entirely different mechanism [19] - [21] . Cu(II) acetylpyridine-thiosemi- carbazone and acetylpyrazine-thiosemicarbazone metal complexes of the general formula [Cu(AP-TSC)Cl] interact with topoisomerase IIα (topoIIα) [20] . Most importantly, these square-planar metal complexes appear to inhibit catalytic activity of topoIIα [20] . The ability to catalytically inhibit topoII is significant in that this avoids the threat often presented by topoII-targeting agents. Most of the commonly-used topoII agents block ligation of cleaved DNA and are known as interfacial poisons, which result in single- and double-stranded DNA breaks [22] . This mechanism is inherently dangerous and sometimes results in therapy-related or secondary leukemias [23] .

An important subset of the α-(N)-heterocyclic thiosemicarbazones is the benzoylpyridine thiosemicarbazones (BZP-TSC’s), based on 2-benzoylpyridine. One problem with the use of many of these α-(N)-heterocyclic thiosemicarbazones as anti-cancer agents is methemoglobin formation, but one group has found that BZP-TSC ligands limit methemoglobin formation [24] . These BZP-TSC ligands have most often been employed as iron chelators because of the overabundance of iron in highly proliferative cells and because of iron’s relatively low cytotoxicity [25] - [28] . Several copper (II) complexes of the benzoylpyridine thiosemicarbazone ligands have been synthesized and characterized [29] - [34] , but their biological activity has not been linked to inhibition of topoIIα. This work provides that crucial link between a series of copper (II) BZP-TSC complexes and inhibition of topoIIα, as well as adding a couple of new BZP-TSC ligands and their copper(II) complexes to the literature. The structures of the BZP-TSC’s and their Cu(II) complexes employed in this work are shown in Figure 1.

2. Experimental

2.1. Materials and Methods

All reagents and solvents used to synthesize the ligands and the copper complexes were purchased from Sigma- Aldrich or Alfa-Aesar. Recombinant human Topoisomerase IIα was overexpressed and purified from yeast Saccharomyces cerevisiae as described [35] . The enzyme was stored in the buffer with 50 mM Tris (pH 7.8), 750 mM KCl, 40% Glycerol and 0.5 mM DTT as 1 mg/mL stock in liquid nitrogen. Recombinant pBR322

Figure 1. Structures and acronyms used for the benzoylpyridine ligands and their copper(II) complexes.

plasmid was amplified and purified following the protocol of Qiagen™ Plasmid Mega Kit.

2.2. Instrumentation

The melting points were taken with a Stanford Research Systems Digimelt MPA160, and TLCs were taken with Whatman 250 μm layer PE SIL G/UV polyester-backed plates. All mass spectrometry data was taken with a Varian 300/310/320-MS LC/MS Quadrupole Mass Spectrometer in negative mode using APCI. The corona current was set to −5.00 μA, while the shield potential was set to −600.00 volts. The housing, drying gas, and vaporizer gas temperatures were set to 50˚C, 150˚C, and 350˚C respectively. The drying, nebulizing, and vaporizer gas pressures were each set to 12.0 psi, 55.0 psi, and 17.0 psi. For the mass spectrometry data, each sample was dissolved in minimal amounts of dimethylsulfoxide and then diluted to 10 ppm in methanol. All Nuclear Magnetic Resonance (NMR) data were collected using an Oxford/Varian 300 MHz NMR. Each 1HNMR was taken over a range of −1 to 16 ppm, with 32 - 64 total scans. The 13CNMR were taken over a range of −20 to 240 ppm and between 1000 - 2000 scans, with an acquisition time of 1.6s and a relaxation delay of 2.0 s. The original data was processed in Vnmrj software, then transferred over to Mestre Nova. UV-Vis data was gathered using a Cary Varian 3E UV-Vis Spectrophotometer. Each sample was diluted to 10 ppm and scanned over a range of 200 - 800 nm.

2.3. Synthesis and Characterization

The ligands BZP-MTSC [34] , BZP-ETSC [34] , BZP-PTSC [32] , and BZP-dMTSC [34] were synthesized by literature procedures, as well as the copper complexes [Cu(BZP-MTSC)Cl] [34] , [Cu(BZP-ETSC)Cl] [34] , [Cu(BZP-PTSC)Cl] [32] , [Cu(BZP-dMTSC)Cl] [34] , and [Cu(APY-ETSC)Cl] [20] .

2.3.1. Synthesis of BZP Ligands

Both ligands were synthesized by the following procedure: In a 50 ml round-bottomed 14/20 necked flask with a magnetic stir bar was added 5.50 mmol of 2-benzoylpyridine and 5.50 mmol of the appropriate thiosemicarbazide (either 4-tertbutyl-3-thiosemicarbazide or 4-benzyl-3-thiosemicarbazide). The reagents were slurried in 25 mL of 2-propanol, and one drop of concentrated sulfuric acid was added to catalyze the reaction. A reflux condensor was attached to the flask, and the reaction mixture was heated to 70˚C and stirred for 24 hours. The off- white precipitate formed was filtered, washed with ethyl ether, and dried under vacuum.

BZP-tBTSC

Yield: 1.53 g (89.8%) MP: 141.5˚C

1H NMR (300 MHz, DMSO-d6, δ): 12.94 (s, 3H, hydrazinic N-H), 8.87 (ddd, J = 4.9, 0.8, 0.9 Hz, 3H, Ar-H), 8.48 (ddd J = 4.8, 1.8, 0.9 Hz, 1H, Ar-H), 8.18 (dt, J = 8.1, 1.1 Hz, 1H, Ar-H), 8.06 - 7.98 (m, 5H, Ar-H), 7.91 (ddd, J = 8.1, 7.5, 1.8 Hz, 1H, Ar-H), 7.84 (s, 3H, thioamide N-H), 7.68 - 7.39 (m, 21H, Ar-H), 7.46 - 7.27 (m, 5H, Ar-H), 1.53 (s, 26H, tert-butyl C-H). 13C NMR (75 MHz, DMSO-d6, δ): 175.99 (N-C-N), 151.92 (Ar), 149.29 (Ar), 142.62 (Ar), 138.73 (tert butyl C-C-H), 137.52 (Ar), 129.76 (Ar), 129.25 (Ar), 129.07 (Ar), 126.69, 125.42 (Ar), 53.44 (tert-butyl C-H), 28.78. Anal. Found: C, 65.13; H, 6.56. Calcd. for C17H20N4S: C, 65.35; H, 6.45. Theoretical MS m/z (relative intensity): 311.14 (100%). Actual MS m/z (relative intensity): 310.9 (100%).

BZP-BzTSC

Yield: 1.57 g (82.4%) MP: 137.2˚C

1H NMR (300 MHz, DMSO-d6, δ): 12.92 (d, J = 2.2 Hz, 1H, hydrazinic N-H), 9.57 (t, J = 6.3 Hz, 0H, Thioamide N-H), 9.30 (t, J = 6.3 Hz, 2H, thioamide N-H), 8.89 - 8.81 (m, 2H, Ar-H), 8.78 (s, 0H, Ar-H), 8.52 (dtd, J = 8.1, 1.1 Hz, 1H, Ar-H), 8.48 - 8.39 (m, 0H, Ar-H), 7.69 - 7.52 (m, 6H, Ar-H), 7.58 - 7.36 (m, 6H, Ar-H), 7.39 - 7.15 (m, 13H, Ar-H), 4.85 (dd, J = 6.2, 2.4 Hz, 4H, benzyl N-C-H), 3.42 (d, J = 2.5 Hz, 15H, benzyl N-C-H). 13C NMR (75 MHz, DMSO-d6, δ): 178.36 (N-C-N), 151.85 (Ar-C), 149.61 (Ar-C), 149.30 (Ar-C), 149.09 (Ar-C), 143.79 (Ar-C), 139.46 (Ar-C), 139.24 (Ar-C), 138.65 (Ar-C), 137.28 (Ar-C), 136.92 (Ar-C), 129.69 (Ar-C), 129.42 (thioamide N-C-Ar), 129.10 (Ar-C), 128.82 (Ar-C), 128.62 (Ar-C), 127.73 (Ar-C), 127.66 (Ar-C), 127.33 (Ar-C), 127.23(Ar-C), 126.57 (Ar-C), 125.38 (Ar-C), 124.47 (Ar-C), 122.17 (Ar-C), 47.73 (Ar-C). Anal. Found: C, 69.13; H, 5.56. Calcd. for C20H18N4S:C, 69.34; H, 5.24. Theoretical MS m/z (relative intensity): 345.1 (100%). Actual MS m/z (relative intensity): 344.9 (100%).

2.3.2. Synthesis of Copper Complexes

Both Cu(II) complexes were synthesized by the following procedure: In a 50 ml round-bottomed 14/20 necked flask with a magnetic stir bar was added 2.50 mmol of cupric chloride dihydrate which was dissolved in 7.5 mL of methanol. Next a solution of 2.50 mmol of the appropriate thiosemicarbazone ligand (either the BZP-tBTSC or the BZP-BzTSC) in 20 mL of methanol was added. Reaction to form the green product was observed to occur immediately. A reflux condenser was attached to the flask, and the reaction mixture was heated to 75˚C and stirred for 4 hours. The green microcrystalline precipitate formed was filtered, washed with ethyl ether, and dried under vacuum.

[Cu(BZP-tBTSC)Cl]

Yield: 0.579 g (88.5%), TLC Silica/ethyl acetate (Rf): 0.91

Theoretical MS m/z (relative intensity): 413.02 (3.6%), 412.02 (18.1%), 411.02 (8.5%), 410.02 (81.3%), 409.03 (18.6%), 408.2 (100.0%). Actual MS m/z (relative intensity): 413.0 (19.4%), 412.0 (17.6%), 411.0 (82.3%), 410.0 (30.9%), 408.9 (100%), 408.1 (6.9%). Anal. Found: C, 49.55; H, 4.79. Calcd. for C17H19ClCuN4 S:C, 49.75; H, 4.67. UV (thiosemicarbazone complexes) λmax, nm (ε): 421 (30.85), 683 (0.6115).

[Cu(BZP-BzTSC)Cl]

Yield: 0.320 g (82.5%). TLC Silica/ethyl acetate (Rf): 0.90

Theoretical MS m/z (relative intensity): 447.01 (3.6%), 446.00 (17.8%), 445.01 (18.1%), 444.01 (79.3%), 443.01 (24.1%), 442.01 (100.0%). Actual MS m/z (relative intensity): 447.0 (19.7%), 446.0 (22.2%), 445.0 (77.3%), 444.0 (41.6%), 443.0 (100%), 441.9 (12.6%). Anal. Found: C, 53.53; H, 3.79. Calcd. for C20H17 ClCuN4S: C, 54.05; H, 3.86. UV (thiosemicarbazone complexes) λmax, nm (ε): 423 (35.11), 684 (0.6340).

2.4. TopoIIα Mediated Relaxation Assay

Plasmid DNA relaxation assays were performed as previously described [36] . Briefly, reactions were set up in a 20 μL volume with 0.5 μg topoIIα, 0.3 μg pBR322, 2 mM ATP in the presence of reaction buffer with 50 mM Tris (pH7.9), 875 mM KCl, 0.5 mM EDTA, 25 mM MgCl2 and 12.5% glycerol. Different concentrations (10, 25, 50 μM) of TSC compounds were incubated with the above reaction mixture at 37˚C for 30 minutes. Control reactions were performed in the presence of 10% DMSO. The reactions were then terminated with addition of 3 μL of stop solution with 77.5 mM EDTA and 0.77% SDS. The proteins in the reaction were digested with by 2 μL of 0.8 mg/ml proteinase K for 30 minutes at 45˚C. The products were then mixed with 2 μL of 6x gel loading dye from Research Products International Corp. (Mount Prospect, IL) and resolved by 1% TBE agarose gel electrophoresis. The gels were stained in 0.5 μg/ml ethidium bromide and visualized by Bio-Rad Gel Doc XR+ imaging system.

2.5. Methods for Alamar Blue Viability Assay of Cells Exposed to Topoisomerase II Inhibitors

2.5.1. Cell Culture and Plating

Cell lines used were MDA-MB-231 (cancerous, epithelial-type human breast adenocarcinoma cells), and MCF7 (cancerous, epithelial-type human breast adenocarcinoma cells). Adherent cells were cultured in 25 cm2 or 75 cm2 tissue culture flasks within a humidified incubator at 37˚C and 5% CO2. DMEM containing L-glutamine (Lonza) supplemented with penicillin/streptomycin and 10% FBS was used as the complete culture medium. Confluent cells were trypsinized, cell clumps dissociated by gentle pipetting, and split using a 1:10 ratio into fresh flasks containing pre-warmed complete media. Cell counts were performed by staining 100 µL of cells with 100 µL of trypan blue (Hyclone) and then counted on a hemocytometer. With this number determined, cells were diluted in complete media to a concentration 25,000 cells/mL, and 200 µL/well of a cell solutions was added to a 96-well plate using a multichannel pipette. Plated cells were placed in the humidified incubator at 37°C and 5% CO2 to incubate for 24 hr prior to drug addiction.

2.5.2. Viability Assay of the Copper Complexes

After cells were cultured for 24 hr, after which the existing culture media was replaced with complete media containing dilutions of compounds [Cu(APY-ETSC)Cl] and [Cu(BZP-tBTSC)Cl] ranging from 100 µM and 0.001 µM. The dilutions were generated using serial dilutions of prepared DMSO stock solutions of 10 mM [Cu(APY-ETSC)Cl] and 10 mM [Cu(BZP-tBTSC)Cl] in ACS-grade DMSO (Fischer) which were stored at −20°C until use. Wells were also prepared with 200 µL of complete media only or 200 µL of complete media with 1:100 dilution of DMSO as positive controls. Both positive controls were found to be statistically equivalent showing no added toxicity of DMSO at the highest applied concentration of DMSO. The 96-well plate was then incubated (humidified, 37°C, 5% CO2) for 24 hr after application of the compounds. Alamar Blue (Thermo Scientific) was then added to each well using complete media as a carrier to attain 8% alamar blue per well and applied using a multichannel pipette. The plate was then placed back into the incubator and allowed to incubate for approximately 5 hr, after which fluorescence measurements were made at 560 nm excitation and 590 nm emission using the Tecan infinite M200 Pro reader.

2.5.3. Data Analysis

Initial analysis of fluorescence data were performed with Microsoft Excel 2013 normalized by the fluorescence signal from positive controls. Statistical analysis, data fitting, and plot generation was performed with GraphPad Prism 6; normalized data were fit using log (inhibitor) vs. response with variable slope (four parameter) model. This model calculated EC50 and Hill slope values for each curve.

3. Results and Discussion

The synthesis of the BZP-TSC ligands has been documented for over two decades, and the ligands act primarily as monoanionic tridentate chelating ligands with a variety of metal ions. Copper(II) complexes of the BZP-TSC ligands synthesized from copper (II) chloride are paramagnetic square-planar complexes in solution as has been determined previously, and we find evidence that the [Cu(BZP-tBTSC)Cl] and the [Cu(BZP-BzTSC)Cl] have the same solution structure. Because of the history of biological properties of the copper complexes, we decided to investigate the possibility that they are involved in inhibition of Topoisomerase IIα, just as the structurally similar acetylpyridine complexes of formula [Cu(APY-TSC)Cl] have recently been discovered to be inhibitors of that important enzyme.

3.1. TopoIIα Mediated Plasmid Relaxation

The ligands and Cu(II) complexes of BZP-TSCs with different substituents were examined in a topoIIα-med- iated plasmid relaxation assay. Without the presence of any compounds (ND), topoIIα relaxed the supercoiled (SC) plasmid pBR322 to the relaxed (R) form as shown in lane 2 of Figure 2. Addition of 50 μM of BZP- MTSC, BZP-ETSC, BZP-tBTSC, BZP-BzTSC, BZP-PTSC or BZP-dMTSC had minor to no effect on inhibiting plasmid relaxation when compared lanes 3 - 8 with lane 2. By constrast, 50 μM of Cu(BZP-MTSC)Cl, Cu (BZP-ETSC)Cl, Cu(BZP-tBTSC)Cl, Cu(BZP-BzTSC)Cl, Cu(BZP-PTSC)Cl or Cu(BZP-dMTSC)Cl significantly inhibits topoIIα-mediated relaxation of supercoiled DNA as indicated in lanes 9 - 14. Our data show that the BZP-TSC ligand has little effect on topoIIα activity while Cu(BZP-TSC)Cl with different substituents all showed inhibitory effect on topoIIα, which is consistent with our studies of other TSC complexes [36] .

We also examined the dose-dependence of [Cu(BZP-tBTSC)Cl] inhibition on topoIIα as shown in Figure 3. Lane 1 shows supercoiled plasmid DNA, and Lane 6 shows topoIIα?mediated relaxed plasmid DNA. Lanes 2 -

Figure 2. Copper(II) BZP-TSC complexes inhibit the plasmid relaxation mediated by human topoIIa. Lane 1, reaction without topoIIa (NT). Lane 2, reaction without compounds but with 10% DMSO (ND). Lanes 3 - 8. BZP-TSC ligands with methyl, ethyl, tert-butyl, benzyl, phenyl and dimethyl substituents inhibits topoIIa slightly at a concentration of 50 mM. Lanes 9 - 14, [Cu(BZP-TSC)Cl] with methyl, ethyl, tert-butyl, benzyl, phenyl and dimethyl substitutions inhibits topoIIa at a concentration of 50 mM.

Figure 3. [Cu(BZP-tBSC)Cl] inhibit the topoIIa-mediated plasmid relaxation in a dose-dependent manner. Lane 1, NT, control reaction without topoIIa. Lanes 2 - 3, [Cu(BZP-tBSC)Cl] inhibits TopoIIa- mediated plasmid relaxation at a concentration of 10, 25, and 50 mM. Lane 5, etoposide weakly inhibits topoIIa-mediated plasmid relaxa- tion at a concentration of 50 mM. Lane 6, ND, control reactions without TSC’s.

4 show inhibition of the topoIIα enzyme in a dose dependent manner from 10 μM solution of [Cu(BZP-tBTSC) Cl] up to a 50 μM solution. Our experiment shows that as little as 10 μM of [Cu(BZP-tBTSC)Cl] can partially inhibit topoIIα activity and that even at this concentration it exhibits more inhibition than does etoposide at a 50 μM solution concentration as seen in Lane 5. It should be noted that etoposide is a widely-used anticancer agent.

3.2. In Vitro Viability Assays

The in vitro viability assay of compounds [Cu(APY-ETSC)Cl)] and [Cu(BZP-tBTSC)Cl] was determined using two cancerous, epithelial-type human breast adenocarcinoma cell lines, MDA-MB-231 and MCF7. Compound concentrations ranging from 0.001 - 100 µM were used and cell viability were assayed after 24 hours of incubation with the compounds in complete DMEM media. The fluorometric, metabolic compound Alamar Blue was used to determine cellular viability. As shown in Figure 4, the effective concentrations causing 50% cell death (EC50) for MDA-MB-231 cells with [Cu(APY-ETSC)Cl] and [Cu(BZP-tBTSC)Cl] is 3.33 µM and 1.01 µM respectively.

In all cases, the viability of the cells decreased with increasing concentration of the compounds. Compound [Cu(APY-ETSC)Cl] generally had a lower effective concentration compared to [Cu(BZP-tBTSC)Cl], but the differences were not determined to be statistically significant in these assays. The MDA-MB-231 cells had an abrupt change in viability after a certain threshold concentration of applied compounds, perhaps due to the physiology of these cells. GraphPad Prism was used to calculate the (EC50) for the various cell types.

As shown in Figure 5, the EC50 for MCF7 cells with [Cu(APY-ETSC)Cl] and [Cu(BZP-tBTSC)Cl] is 0.0558 µM and 0.248 µM respectively. Previously reported viability data for [Cu(APY-ETSC)Cl] with MCF7 indicated higher EC50 values [20] , but this difference is most likely due to the lower cellular concentration used in our assays. Approximately 5000 cells per well were used in these assays to allow for expansion of the cells in the 96 well plate format, as opposed to 50,000 cells per well, which would cause over confluent conditions in our assay.

4. Conclusions

Thiosemicarbazones that are of the a-(N)-heterocyclic thiosemicarbazone class, such as the BZP-TSC’s in this study, have proven to be a special class of ligands with many biological properties. The ligands themselves are often potent ribonucleotide reductase inhibitors (such as Triapine), but have very little inhibitory effect against topoIIa. However, we show in this study that the copper(II) complexes of those ligands with the formula [Cu(BZP-TSC)Cl] are potent topoisomerase IIa inhibitors, just as has been shown to be the case for the analogous copper(II) complexes of the formylpyridine thiosemicarbazones, [Cu(FP-TSC)Cl], acetylpyridine thiosemicarbazones, [Cu(APY-TSC)Cl], and acetylpyrazine thiosemicarbazones, [Cu(APZ-TSC)Cl]. The alkyl and aryl

Figure 4. Viability curve for MDA-MB-231 cells with compounds [Cu(APY-ETSC)Cl] (solid circles) and [Cu(BZP-tBTSC)Cl] (solid squares). The calculated EC50 for [Cu(APY-ETSC)Cl] and [Cu (BZP-tBTSC)Cl] is 3.33 µM and 1.01 µM respectively.

Figure 5. Viability curve for MCF7 cells with compounds [Cu(APY- ETSC)Cl] (solid circles) and [Cu(BZP-tBTSC)Cl] (solid squares). The calculated EC50 for [Cu(APY-ETSC)Cl] and [Cu(BZP-tBTSC) Cl] is 0.0558 μM and 0.248 μM respectively.

substituents on the end of the thiosemicarbazone ligand, such as methyl, ethyl, tert-butyl, phenyl, etc., seem to have no real effect on the efficacy of the copper(II) complexes to inhibit the action of human topoIIα as we have determined in this study.

If the substituents on the thiosemicarbazone seem to have no real effect, and the imine portion of the copper complex can be either a formyl, acetyl, or even a benzoyl group, as we have shown here, then the question of the mechanism of action of the copper complexes to inhibit topoIIα now seems to focus on the square-planar geometry of these complexes, which is rare for copper (II), and the nature of the Cu-Cl bond. Studies in the future may address the question of whether or not the [Cu(BZP-TSC)Cl] complexes react with the topoIIα enzyme to become five or six coordinate, and whether or not the Cu-Cl bond is involved in this process.

In summary, our results demonstrate that Cu(II) BZP-TSC complexes are better topoIIα inhibitors compared with the BZP-TSC ligands and that the [Cu(BZP-tBTSC)Cl] complex inhibits topoIIα in a dose-dependent way. Additionally, the [Cu(BZP-tBTSC)Cl] complex also appears to be more effective at killing MDA-MB-231 and MCF7 breast cancer cell lines than does [Cu(APY-ETSC)Cl].

Acknowledgements

J.D.C. thanks the TTU Chemistry Department for a Student Research Grant, and M.T.S. thanks the TTU URECA! Program for a research mini-grant.

NOTES

*Corresponding author.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Conner, J. , Medawala, W. , Stephens, M. , Morris, W. , Deweese, J. , Kent, P. , Rice, J. , Jiang, X. and Lisic, E. (2016) Cu(II) Benzoylpyridine Thiosemicarbazone Complexes: Inhibition of Human Topoisomerase IIα and Activity against Breast Cancer Cells. Open Journal of Inorganic Chemistry, 6, 146-154. doi: 10.4236/ojic.2016.62010.

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