Evaluation of Volatile Compounds of “Tiger Nut Beverage” (Orxata de Xufla) Headspace by Optimized Solid-Phase Micro-Extraction

DOI: 10.4236/oalib.1101015   PDF        1,369 Downloads   1,853 Views   Citations


The aroma profile of “tiger nut beverage” (orxata de xufla) was investigated by headspace solid-phase micro-extraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS). Polidimethilsiloxane /divinylbenzene (PDMS/DVB), carboxen/polydimethylsiloxane (CAR/ PDMS), polyacrylate (PA) and divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fibers were tested and compared for sensitivity beyond conditions that might affect the SPME procedure, such as temperature, concentration of ions and sample volume. The SPME fiber coated with DVB/CAR/PDMS presented the most efficient extraction compounds from the headspace of the product, particularly when the volatiles were extracted at 60℃ with 2 mL of the sample volume. The addition of salt did not show any significant advantage for the qualification of the volatile fraction. Eighty-three compounds were identified or tentatively identified by GC-MS, being terpenes the most abundant group.

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Klein, B. , Gallardo-Chacón, J. , Codina-Torrella, I. , Trujillo, A. and Juan, B. (2014) Evaluation of Volatile Compounds of “Tiger Nut Beverage” (Orxata de Xufla) Headspace by Optimized Solid-Phase Micro-Extraction. Open Access Library Journal, 1, 1-15. doi: 10.4236/oalib.1101015.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Ferragut, V., Hernández-Herrero, M., Poliseli, F., Valencia, D. and Guamis, B. (2011) Ultra High Pressure Homogenization (UHPH) Treatment of Vegetable Milks: Improving Hygienic and Colloidal Stability. Proceedings of the 11th International Congress on Engineering and Food, Athens, 22-26 May 2011, 40-41.
[2] (2013) Ministerio de Agricultura, Alimentación y Mediambiente. Superfícies y producciones de cultivo.
[3] (2013) CRDO Consejo Regulador de la Denominación de Origen Chufa de Valencia.
[4] Corrales, M., Souza, P.M., Stahl, M.R. and Fernández, A. (2012) Effects of the Decontamination of a Fresh Tiger Nut Milk Beverage (Horchata) with Short Wave Ultraviolet Treatments (UV-C) on Quality Attributes. Innovative Food Science & Emerging Technologies, 13, 163-168.
[5] Cortés, C., Esteve, J.M., Frigola, A. and Torregros, F. (2005) Quality Characteristics of Horchata (a Spanish Vegetable Beverage) Treated with Pulsed Electric Fields during Shelf-Life. Food Chemistry, 91, 319-325.
[6] Perez-Cacho, P.R. and Rouseff, R. (2008) Processing and Storage Effects on Orange Juice Aroma. A Review. Journal of Agricultural and Food Chemistry, 56, 9785-9796.
[7] Bazemore, R., Goodner, K.L. and Rouseff, R.L. (1999) Volatiles from Unpasteurized and Excessively Heated Orange Juice Analyzed with Solid Phase Microextraction and GC-Olfactometry. Journal of Food Science, 64, 800-803.
[8] Ducki, S., Miralles-Garcia, J. and Zumbe, A. (2008) Evaluation of Solid-Phase Micro-Extraction Coupled to Gas Chromatography-Mass Spectometry for the Headspace Analysis of Volatile Compounds in Cocoa Products. Talanta, 74, 1166-1174.
[9] Kataoka, H., Lord, H.L. and Pawliszyn, J. (2002) Applications of Solid-Phase Microextraction in Food Analysis. Journal of Chromatography A, 880, 35-62.
[10] McLafferty, F.W. (2000) Wiley Registry of Mass Spectral Data. 6th Edition.
[11] R Development Core Team (2005) R Foundation for Statistical Computing. Vienna.
[12] Geerdink, R.B., Breidenbach, R. and Epema, O.J. (2007) Optimization of Headspace Solid-Phase Microextraction Gas Chromatography-Atomic Emission Detection Analysis of Monomethylmercury. Journal of Chromatography A, 1174, 7-12.
[13] Nongonierma, A., Cayot, P., Le Quéré, J.L., Springett, M. and Voilley, A. (2006) Mechanisms of Extraction of Aroma Compounds from Foods, Using Adsorbents: Effect of Various Parameters. Food Reviews International, 22, 51-94.
[14] Machields, D. and Istasse, L. (2003) Evaluation of Two Commercial Solid-Phase Microextraction Fibers for the Analysis of Target Aroma Compounds in Cooked Beef Meat. Talanta, 61, 529-537.
[15] Prosen, H. and Zupancic-Kralj, L. (2002) Solid Phase Microextraction. Trends in Analytical Chemistry, 18, 272-282.
[16] Guichard, E. (2000) Interactions between Flavor Compounds and Food Ingredients and Their Influence on Flavor Perception. Food Reviews International, 18, 49-70.
[17] Potineni, R.V. and Peterson, D.G. (2005) Influence of Thermal Processing Conditions on Flavor Stability in Fluid Milk: Benzaldehyde. Journal of Dairy Science, 88, 1-6.
[18] Fernandez, X., Kerverdo, S., Duñach, E. and Lizzani-Cuvelier, L. (2002) Les hétérocycles dans la chimie des arômes. In: Société Française de Chimie, Ed., L’Actualité Chimique, Paris.
[19] Serhan, M., Linder, M., Hosri, C. and Fanni, J. (2010) Changes in Proteolysis and Volatile Fraction during Ripening of Darfiyeh, a Lebanese Artisanal Raw Goat’s Milk Cheese. Small Ruminant Research, 90, 75-82.
[20] Kazeniac, S.J. and Hall, R.M. (1970) Flavor Chemistry of Tomato Volatiles. Journal of Food Science, 35, 519-530.
[21] Maga, J.A. and Katz, I. (1979) Furans in Foods. Critical Reviews in Food Science and Nutrition, 11, 355-400.
[22] Owczarek-Fendor, A., Meulenaer, B., Scholl, G., Adams, A., Van Lancker, F., Eppe, G., et al. (2010) Furan Formation in Starch-Based Model Systems Containing Carbohydrates in Combination with Proteins, Ascorbic Acid and Lipids. Food Chemistry, 133, 816-821.
[23] Hodge, J.E., Mills, J.D. and Fischer, B.E. (1972) Compounds from Browned Flavour Derived from Sugar-Amine Reactions. Cereal Science Today, 17, 34-38.
[24] Keyhani, A. and Yalayan, V.A. (1996) Pyrolysis/GC/MS Analysis of N-(1-Deoxy-D-Fructos-1-yl)-L-Phenylalanine: Identification of Novel Pyridine and Naphthalene Derivatives. Journal of Agricultural and Food Chemistry, 44, 223-229.
[25] Wu, Y., Zhu, B., Tu, C., Duan, C.Q. and Pan, Q.H. (2011) Generation of Volatile Compounds in Litchi Wine during Winemaking and Short-Term Bottle Storage. Journal of Agricultural and Food Chemistry, 59, 4923-4931.
[26] Ren, B. (2003) Atom-Type-Based AI Topological Descriptors for Quantitative Structure Retention Index Correlations of Aldehydes and Ketones. Chemometrics and Intelligent Laboratory Systems, 66, 29-39.
[27] Liu, C., Cheng, Y., Zhang, Y.H., Deng, X., Chen, F. and Xu, J. (2012) Volatile Constituents of Wild Citrus Mangshanyegan (Citrus nobilis Lauriro) Peel Oil. Journal of Agricultural and Food Chemistry, 60, 2617-2628.
[28] Larrayoz, P., Addis, M., Gauch, R. and Bosset, J.O. (2011) Comparison of Dynamic Headspace and Simultaneous Distillation Extraction Techniques Used for the Analysis of the Volatile Components in Three European PDO Ewes’ Milk Cheeses. International Dairy Journal, 11, 911-926.
[29] Hashizume, M., Gordon, M.H. and Mottram, D.S. (2007) Light-Induced Off-Flavor Development in Cloudy Apple Juice. Journal of Agricultural and Food Chemistry, 55, 9177-9182.
[30] Ong, P.K., Acree, T.E. and Lavin, E.H. (1998) Characterization of Volatiles in Rambutan Fruit (Nephelium lappaceum L.). Journal of Agricultural and Food Chemistry, 46, 611-615.
[31] Neffat, M., Sriti, J., Hamdaoui, G., Kchouk, M.E. and Marzouk, B. (2011) Salinity Impact on Fruit Yield, Essential Oil Composition and Antioxidant Activities of Coriandrum sativum Fruit Extracts. Food Chemistry, 124, 221-225.
[32] El-Sayed, A.M. (2012) The Pherobase: Database of Pheromones and Semiochemicals.
[33] Acree, T. and Arn, H. (2004) Flavornet and Human Odor Space.

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