The Function of miR-31 Expression in Differentiation of Neural Stem Cells


Spinal cord injury (SCI) is a severe complication of acute spinal injury, it can lead to axonal degeneration and necrosis of neurons, causing damage cross section below the movement, then loss of sensory, reflex and autonomous control function below the damage section. Nowadays, there is increasing evidence that transplantation of neural stem cells can help to repair spinal cord injury after spinal cord injury. The aim of this study was to research the function of miR-31 expression in differentiation of neural stem cells. Mir-31 was transfected the mimics and inhibitor into neural stem cells. By the morphological observation, the cell over expression of miR-31 was closer to NSCs. With the RT-PCR, Hb9 and STMN1 were up-regulate when miR-31 was inhibited, and Nestin was down-regulate. When miR-31 was over expression, the expression of Nestin and Hb9 was up-regulate. These findings suggest that miR-31 may play a significant role in proliferation and differentiation of neural stem cell and are potential targets for therapeutic interventions following spinal cord injury.

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Li, X. , Gao, Y. , Li, P. , Tian, F. , Wang, J. and Wang, C. (2016) The Function of miR-31 Expression in Differentiation of Neural Stem Cells. Journal of Biosciences and Medicines, 4, 57-64. doi: 10.4236/jbm.2016.43010.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Lu, P., Woodruff, G., Wang, Y.Z., Hunt, L.G.M., Wu, D., Boehle, E., Ahmad, R., Poplawski, G., Brock, J., Goldstein, L.S.B. and Tuszynski, M.H. (2014) Long-Distance Axonal Growth from Human Induced Pluripotent Stem Cells after Spinal Cord Injury. Neuron, 83, 789-796.
[2] Suzuki, S.O. and Goldman, J.E. (2003) Multiple Cell Populations in the Early Postnatal Subventricular Zone Take Distinct Migratory Pathways: A Dynamic Study of Glial and Neuronal Progenitor Migration. J Neurosci, 23, 4240-4250.
[3] Harraz, M.M., Xu, J.-C. and Guiberson, N. (2014) miR-223 Regulates the Differentiation of Immature Neurons. Mol Cell Ther., 2, 1-15.
[4] Niu, C.S., Yang, Y. and Cheng, C.-D. (2013) miR-134 Regulates the Proliferation and Invasion of Glioblastoma Cells by Reducing Nanog Expression. 42, 1533-1540.
[5] Mor, E., Kano, S.-I., Colantuoni, C., Sawa, A., et al. (2013) microRNA-382 Expression Is Elevated in the Olfactory Neruoepithelium of Schizophrenia Patients. 55, 1-10.
[6] Wei, H.E., Wang, C.F., Zhang, C.S., Li, P.F., Wang, F. and Zhang, Z.Y. (2010) Comparative Profiling of microRNA Expression between Neural Stem Cells and Motor Neurons in Embryonic Spinal Cord in Rat. Int. J. Devl Neuroscience, 28, 545-551.
[7] Wong, T.S., Liu, X.B., Wong, B.Y., Ng, R.W., Yuen, A.P. and Wei, W.L. (2008) MaturemiR-184 as Potential Oncogenic microRNA of Squamous Cell Carcinoma of Tongue. Clin Cancer Res, 14, 2588-2592.
[8] Motoyama, K., Inoue, H., Takatsuno, Y., Tanaka, F., Mimori, K., Uetake, H., Sugihara, K. and Mori, M. (2009) Over- and Under-Expressed microRNAs in Human Colorectal Cancer. Int J Oncol, 34, 1069-1075.
[9] Wang, C.J., Zhou, Z.G., Wang, L., Yang, L., Zhou, B., Gu, J., Chen, H.Y. and Sun, X.F. (2009) Clinicopathological Significance of microRNA-31, -143 and -145 Expression in Colorectal Cancer. Dis Markers, 26, 27-34.
[10] Liu, X., Sempere, L.F., Ouyang, H., Memoli, V.A., Andrew, A.S., Luo, Y., Demidenko, E., Korc, M., Shi, W., Preis, M., Dragnev, K.H., Li, H., Direnzo, J., Bak, M., Freemantle, S.J., Kauppinen, S. and Dmitrovsky, E. (2010) MicroRNA-31 Functions as an Oncogenic microRNA in Mouse and Human Lung Cancer Cells by Repressing Specific Tumor Suppressors. J Clin Invest, 120, 1298-1309.
[11] Valastyan, S., Benaich, N., Chang, A., Reinhardt, F. and Weinberg, R.A. (2009) Concomitant Suppression of Three Target Genes Can Explain the Impact of a microRNA on Metastasis. Genes Dev., 23, 2592-2597.
[12] Schaefer, A., Jung, M., Mollenkopf, H.J., Wagner, I., Stephan, C., Jentzmik, F., Miller, K., Lein, M., Kristiansen, G. and Jung, K. (2010) Diagnostic and Prognostic Implications of microRNA Profiling in Prostate Carcinoma. Int J Cancer, 126, 1166-1176.
[13] Guo, J., Miao, Y., Xiao, B., Huan, R., Jiang, Z., Meng, D. and Wang, Y. (2009) Differential Expression of microRNA Species in Human Gastric Cancer Versus Non-Tumorous Tissues. J Gastroenterol Hepatol, 24, 652-657.
[14] Zhang, Y., Guo, J., Li, D., Xiao, B., Miao, Y., Jiang, Z. and Zhuo, H. (2010) Down-Regulation of miR-31 Expression in Gastric Cancer Tissues and Its Clinical Significance. Med Oncol, 27, 685-689.
[15] Park, D., Xiang, A.P., Mao, F.F., Zhang, L., Di, C.G., Liu, X.M., Shao, Y., Ma, B.F., Lee, J.H., Ha, K.S., Walton, N. and Lahn, B.T. (2010) Nestin Is Required for the Proper Self-Renewal of Neural Stem Cells. Stem Cells, 28, 2162- 2171.
[16] Yamada, K., Matsuzaki, S., Hattori, T., Kuwahara, R., Taniguchi, M., Hashimoto, H., Shintani, N., Baba, A., Kumamoto, N., Yamada, K., Yoshikawa, T., Katayama, T. and Tohyama, M. (2010) Increased Stathmin1 Expression in the Dentate Gyrus of Mice Causes Abnormal Axonal Arborizations. PLoS One, 5, e8596.
[17] Ozon, S., Guichet, A., Gavet, O., Roth, S. and Sobel, A. (2002) Drosophila Stathmin: A Microtubule-Destabilizing Factor Involved in Nervous System Formation. Mol Biol Cell, 13, 698-710.
[18] Hassan, M.K., Watari, H., Mitamura, T., Mohamed, Z., El-Khamisy, S.F., Ohba, Y. and Sakuragi, N. (2015) P18/Stath-min1 Is Regulated by miR-31 in Ovarian Cancer in Response to Taxane. Oncoscience, 2, 294-308.

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