Metal Salts Assisted Enzyme-Based Extraction of Stevioside from the Leaves of Stevia rebaudiana Bertoni

A. Bhaskar Rao; Sara A. George; Sateesh Alavala; H. M. Meshram; K. Chandra Shekar

doi:10.4236/abb.2015.612075

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Advances in Bioscience and Biotechnology > Vol.6 No.12, December 2015

Metal Salts Assisted Enzyme-Based Extraction of Stevioside from the Leaves of Stevia rebaudiana Bertoni ()

A. Bhaskar Rao^*, Sara A. George, Sateesh Alavala, H. M. Meshram, K. Chandra Shekar

Medicinal Chemistry and Pharmacology Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, India.

DOI: 10.4236/abb.2015.612075 PDF HTML XML 4,640 Downloads 5,357 Views Citations

Abstract

Stevioside, extracted from the leaves of Stevia rebaudiana Bertoni, is a natural, high intensity, lowcaloric sweetener with wide therapeutic activities. Conventional stevioside extraction methodologies involve the use of non-green solvents, supercritical fluids, microwaves, etc., however, all these processes are expensive, time-consuming and eco-unfriendly. Therefore an alternative process is desired for the isolation of stevioside. In this study, a novel enzyme-mediated extraction (EME) method has been developed. The dry stevia leaves were pre-treated with hydrolytic enzymes aided by transition metal salts (FeCl3). This was followed by pressurized hot water extraction (PHWE) to release stevioside. The crude extract was purified and clarified through multi-stage membrane filtration. The results confirm that metal salt-assisted cellulase pre-treatment enhanced the yield of stevioside to 72%, with 98% purity, which was higher in comparison to existing methods. Thus, the methodology developed establishes a simple, “green”, enzyme-mediated process for the efficient isolation of stevioside under economical and eco-friendly conditions.

Keywords

Stevia rebaudiana, Enzyme-Based Extraction, Membrane Filtration, Stevioside, Natural Sweetener

Share and Cite:

Rao, A. , George, S. , Alavala, S. , Meshram, H. and Shekar, K. (2015) Metal Salts Assisted Enzyme-Based Extraction of Stevioside from the Leaves of Stevia rebaudiana Bertoni. Advances in Bioscience and Biotechnology, 6, 734-743. doi: 10.4236/abb.2015.612075.

Conflicts of Interest

The authors declare no conflicts of interest.

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