Analgesic Activities of Methanol Extract of Terminalia chebula Fruit

Shadab Ahmed¹, Auroj Naved¹, Rafeeq Alam Khan^1*, Shahida Siddiqui²

¹1Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
²Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.

DOI: 10.4236/pp.2015.612056                

Abstract

Pain is a sensation which in many cases represents the only symptom for the diagnosis of several disorders. Throughout ages man has used many different remedies for pain relief, among which medicinal herbs are most common due to their efficacy and relative safety due to their natural origin. In present study methanol extract of Terminalia chebula fruit was evaluated for its analgesic effects in albino mice. The extract was prepared using whole dried powdered fruit with seeds in absolute methanol. It was then subjected to rotary evaporator under reduced pressure to get concentrated extract; different doses (300, 500 and 1000 mg/kg) were assessed for analgesic activity by tail immersion technique and acetic acid induced writhing test. Similar doses of Aspirin were used as standard drug (positive control) for comparison. Results of both tail flick method and acetic acid induced writhing test revealed that T. chebula fruit extract possessed varying degree of analgesic activity significant at 300 mg/kg and highly significant at 500 and 1000 mg/kg in comparison to control. The results were almost similar to standard drug. In acetic acid induced writhing test, maximum inhibition of writhing was observed at 1000 mg/kg where the number of writhes decreased from 14.1 to 5.2 indicating 63.1% inhibition. Above findings suggest that T. chebula fruit extract possesses significant analgesic activity in albino mice with reference to positive and negative control groups; however further studies on a large number of animals with clinical trials are required to confirm safe and effective use of this fruit extract in humans for analgesic purpose.

Keywords

Terminalia chebula, Methanol Extract, Pain, Tail Flick Test, Writhing Test

Conflicts of Interest

The authors declare no conflicts of interest.

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