Crude Protein and Proline in Dry Bean Seed Respond to Weeding and Soil Fertility Regimes


The objective of this study was to investigate the effect of weeds and fertilizer application on dry bean seed quality. Four dry bean (Phaseolus vulgaris L.) cultivars, Caledon (C), Ukulinga (U), Gadra (G) and uMtata (M) were planted for seed production using a field experiment designed as a split, replicated three times. There were three levels of weeding W0 (no weeding), W1 (weeding until 50% flowering) and W2 (weeding all the time until harvest). The weeding treatments were split into no fertilizer application (F0) and optimum fertilizer application (F1) according to soil fertility analysis. At harvest maturity, seeds were compared for quality with respect to size, germination and total protein content. Proline content was determined as a measure of crop response to the weed and fertilizer stresses during crop production. Although seed size was affected by management stress, seed germination was not significantly affected by weeding and fertilizer even when it was explored in terms of seed vigor by determining rate of germination and seedling size. However, weed management and fertilizer application significantly affected proline and total crude protein contents in seeds (P < 0.05). The findings of this study show that the biotic stress of weeds and abiotic stress of soil fertility can be used to determine seed physiological quality of dry bean seeds.

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P. Miya, S. and T. Modi, A. (2015) Crude Protein and Proline in Dry Bean Seed Respond to Weeding and Soil Fertility Regimes. American Journal of Plant Sciences, 6, 2811-2818. doi: 10.4236/ajps.2015.618277.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Goodwin, M. (2003) Crop Profile for Dry Beans.
[2] Mkanda, A.V., Minnaar, A. and de Kock, H.L. (2007) Relating Consumer Preferences to Sensory and Physicochemical Properties of Dry Beans (Phaseolus vulgaris). Journal of the Science of Food and Agriculture, 87, 2868-2879.
[3] Singh, S.P., Teran, H., Munoz, C.G., Osorno, J.M., Takegami, J.C. and Thung, M.D. (2003) Low Soil Fertility Tolerance in Landraces and Improved Common Bean Genotypes. Crop Science, 43, 110-119.
[4] Escribano, M.R., Santalla, M. and de Ron, A.M. (1997) Genetic Diversity in Pod and Seed Quality Traits of Common Bean Populations form North-Western Spain. Euphytica, 93, 71-81.
[5] Broughton, W.J., Hernandez, G., Blair, M., Beebe, S., Gepts, P. and Vanderleyden, J. (2003) Beans (Phaseolus spp.)— Model Food Legumes. Plant and Soil, 252, 55-128.
[6] DAFF (Department of Agriculture, Forestry and Fisheries) (2010) Dry Beans.
[7] Black, M. and Halmer, P. (2006) The Encyclopedia of Seeds: Science, Technology and Uses. CABI, Wallingford.
[8] Buhler, D.D. (2002) Challenges and Opportunities for Integrated Weed Management. Weed Science, 50, 273-280.[0273:AIAAOF]2.0.CO;2
[9] Mashingaidze, A.B. (2004) Improving Weed Management and Crop Productivity in Maize Systems in Zimbabwe. PhD Dissertation, Wageningen University, Wageningen.
[10] McDonald, G.K. and Gill, G.S. (2009) Improving Crop Competitiveness with Weeds: Adaptations and Trade-Offs. Elsevier Inc., Academic Press, San Diego.
[11] Donald, W.W. (2000) Alternative Ways to Control Weeds between Rows in Weeded Check Plots in Corn (Zea mays) and Soybean (Glycine max). Weed Technology, 14, 36-44.[0036:AWTCWB]2.0.CO;2
[12] Wicks, G.A., Johnston, D.N., Nuland, D.S. and Kinbacher, E.J. (1973) Competition between Annual Weeds and Sweet Spanish Onions. Weed Science, 21, 436-439.
[13] Pynenburg, G.M., Sikkema, P.H. and Gillard, C.L. (2011) Agronomic and Economic Assessment of Intensive Pest Management of Dry Bean (Phaseolus vulgaris). Crop Protection, 30, 340-348.
[14] Liebman, M. and Davis, A.S. (2000) Integration of Soil, Crop and Weed Management in Low-External-Input Farming Systems. Weed Research, 40, 27-47.
[15] Barberi, P. (2002) Weed Management in Organic Agriculture: Are We Addressing the Right Issues? Weed Research, 42, 177-193.
[16] Buhler, D.D. (2004) Weed Biology, Cropping Systems, and Weed Management. Journal of Crop Production, 8, 245-270.
[17] Michigan State University Extension (2015) Integrated Pest Management.
[18] Patterson, D.T. (1995) Effects of Environmental Stress on Weed/Crop Interactions. Weed Science, 43, 483-490.
[19] Rahmati, S., Sajedi, N.A. and Gomarian, M. (2013) Effects of Time Cultivation and Weeds Control Methods on Yield and Yield Components of Red Bean (Phaseolus calcaratus L.). International Journal of Agriculture and Crop Science, 5, 2795-2803.
[20] Bradford, M.M. (1976) A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein—Dye Binding. Analytical Biochemistry, 72, 248-254.
[21] Sowmya, K.J., Gowda, R., Bhanuprakash, K., Yogeesha, H.S., Puttaraju, T.B. and Channakeshava, B.C. (2013) Enhancement of Seed Quality through Chemopriming in Cucumber (Cucumis sativus L.). Mysore Journal of Agricultural Science, 47, 22-30.
[22] ISTA (2015) International Rules for Seed Testing, Vol. 215, Introduction, i-1-6 (10). The International Seed Testing Association, Bassersdorf.
[23] Bates, L.S., Waldren, R.P. and Teare, I.D. (1973) Short Communication: Rapid Determination of Free Proline for Water-Stress Studies. Plant and Soil, 39, 205-207.
[24] Wisiol, K. (1979) Clipping of Water-Stressed Blue Grama Affects Proline Accumulation and Productivity. Journal of Range Management, 32, 194-195.
[25] Szabados, L. and Savoure, A. (2009) Proline: A Multifunctional Amino Acid. Trends in Plant Science, 15, 89-97.
[26] Socias, F.X., Pol, A., Aguiw, F., Vadell, J. and Medrano, H. (1997) Effects of Rapidly and Gradually Induced Water Stress on Plant Response in Subterranean Clover Leaves. Journal of Plant Physiology, 150, 212-219.
[27] Claussen, W. (2005) Proline as a Measure of Stress in Tomato Plants. Plant Science, 168, 241-248.
[28] Celik, O. and Unsal, S.G. (2013) Expression Analysis of Proline Metabolism-Related Genes in Salt-Tolerant Soybean Mutant Plants. Plant Omics Journal, 6, 364-370.
[29] Xiao, G.J., Zhang, F.J., Qiu, Z.J., Yao, Y.B., Wang, R.Y. and Huang, J. (2013) Response to Climate Change for Potato Water Use Efficiency in Semi-Arid Areas of China. Agricultural Water Management, 127, 119-123.
[30] Cvikrova, M., Gemperlova, L., Martincova, O. and Vankova, R. (2013) Effect of Drought and Combined Drought and Heat Stress on Polyamine Metabolism in Proline-over-Producing Tobacco Plants. Plant Physiology and Biochemistry, 73, 7-15.
[31] Xiong, J., Zhang, L., Fu, G.F., Yang, Y.J., Zhu, C. and Tao, L.X. (2012) Drought-Induced Proline Accumulation Is Uninvolved with Increased Nitric Oxide, which Alleviates Drought Stress by Decreasing Transpiration in Rice. Journal of Plant Research, 125, 155-164.
[32] González-Gallegos, E., Laredo-Alcalá, E., Ascacio-Valdés, J., Jasso de Rodríguez, D. and Hernández-Castillo, F.D. (2015) Changes in the Production of Salicylic and Jasmonic Acid in Potato Plants (Solanum tuberosum) as Response to Foliar Application of Biotic and Abiotic Inductors. American Journal of Plant Sciences, 6, 1785-1791.

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