Healing Mechanism and Osteogenic Capacity of Bovine Bone Mineral—Human Amniotic Mesenchymal Stem Celland Autogenous Bone Graft in Critical Size Mandibular Defect

Abstract

Experiments on maxillofacial bone tissue engineering showed the promising result; however, its healing mechanisms and effectiveness had not been fully understood. The aim of this study is to compare the bone healing mechanism and osteogenic capacity between bovine bone mineral loaded with hAMSC and autogenous bone graft in the reconstruction of critical size mandibular bone defect. Critical size defects were made at the mandible of 45 New Zealand white rabbits reconstructed with BBM-hAMSC, BBM alone, and ABG, respectively. At the end of first, second, and twelfth weeks, five rabbits from each experimental week were sacrificed for histology and immunohistochemistry staining. Expressions of vascular endothelial growth factor (VEGF), bone mor-phogenic proteins-2 (BMP2), Runx2 and the amount of angiogenesis were analyzed in the first and second week groups, while expressions of Runx2, osteocalcin, collagen type-I fibres, trabecular area and bone incorporation were analyzed in the twelfth week groups. The result showed that expressions of VEGF, BMP2 and Runx2 as well as the amount of angiogenesis were higher in ABG compared with BBM-hAMSC group in the first and second weeks of healing. The result of twelfth week of healing showed that expressions of Runx2 and osteocalcin as well as the thickness of collagen type-I fibres were significantly higher in BBM-hAMSC compared to ABG group, while there was no statistically difference in trabecular area and bone incorporation between BBM-hAMSC and ABG group. This study concluded that early healing activities were higher in auto-genous bone graft than in BBM-hAMSC, while osteogenic activities in the late stage of healing were higher in BBM-hAMSC compared to autogenous bone graft. It was also concluded that the osteo-genic capacity of BBM-hAMSC was comparable to autogenous bone graft in the reconstruction of critical size defect in the mandible.

Share and Cite:

Kamadjaja, D. , Purwati, Rantam, F. , Ferdiansyah and Pramono, D. (2015) Healing Mechanism and Osteogenic Capacity of Bovine Bone Mineral—Human Amniotic Mesenchymal Stem Celland Autogenous Bone Graft in Critical Size Mandibular Defect. Journal of Biomedical Science and Engineering, 8, 733-746. doi: 10.4236/jbise.2015.810070.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Arrington, E.D., Smith, W.J., Chambers, H.G., Bucknell, A.L. and Davino, N.A. (1996) Complications of Iliac Crest Bone Graft Harvesting. Clinical Orthopaedics and Related Research, 329, 300-309.
http://dx.doi.org/10.1097/00003086-199608000-00037
[2] Heary, R.F., Schlenk, R.P., Sacchieri, T.A., Barone, D. and Brotea, C. (2002) Persistent Iliac Crest Donor Site Pain: Independent Outcome Assessment. Neurosurgery, 50, 510-516.
[3] Bruder, S.P., Kraus, K.H., Goldberg, V.M. and Kadiyala, S. (1998) The Effect of Implants Loaded with Autologous Mesenchymal Stem Cells on the Healing of Canine Segmental Bone Defects. Journal of Bone & Joint Surgery (American Volume), 80, 985-996.
[4] Kon, E., Muraglia, A., Corsi, A., Bianco, P., Marcacci, M. and Martin, I. (2000) Autologous Bone Marrow Stromal Cells Loaded onto Porous Hydroxyapatite Ceramic Accelerate Bone Repair in Critical-Size Defects of Sheep Long Bones. Journal of Biomedical Materials Research, 49, 328-337.
http://dx.doi.org/10.1002/(SICI)1097-4636(20000305)49:3<328::AID-JBM5>3.0.CO;2-Q
[5] Petite, H., Viateau, V., Bensaid, W., Meunier, A., de Pollak, C. and Bourguignon, M. (2000) Tissue-Engineered Bone Regeneration. Nature Biotechnology, 9, 959-963.
http://dx.doi.org/10.1038/79449
[6] Mastrogiacomo, M., Corsi, A., Francioso, E., Di Comite, M., Monetti, F., Scaglione, S., Favia, A., Crovace, A., Bianco, P. and Cancedda, R. (2006) Reconstruction of Extensive Long Bone Defects in Sheep Using Resorbable Bioceramics Based on Silicon Stabilized Tricalciumphosphate. Tissue Engineering, 12, 1261-1273.
http://dx.doi.org/10.1089/ten.2006.12.1261
[7] Kruyt, M.C., Wilson, C.E., de Bruijn, J.D., van Blitterswijk, C.A., Oner, C.F. and Verbout, A.J. (2006) The Effect of Cell-Based Bone Tissue Engineering in a Goat Transverse Process Model. Biomaterials, 29, 5099-5106.
http://dx.doi.org/10.1016/j.biomaterials.2006.05.048
[8] Yue, H., Zhang, Z.Y., Zhu, H.G., Qiu, W.L., Jiang, X.Q. and Guo, W. (2007) Experimental Study on Reconstruction of Segmental Mandible Defects Using Tissue Engineered Bone Combined Bone Marrow Stromal Cells with Three-Dimensional Tricalcium Phosphate. Journal of Craniofacial Surgery, 18, 800-805.
http://dx.doi.org/10.1097/scs.0b013e31806901f5
[9] Yuan, J., Cui, L., Zhang, W.J., Liu, W. and Cao, Y.L. (2007) Repair of Canine Mandibular Bone Defects with Bone Marrow Stromal Cells and Porous Beta Tricalcium Phosphate. Biomaterials, 28, 1005-1013.
http://dx.doi.org/10.1016/j.biomaterials.2006.10.015
[10] Sakaguchi, Y., Sekiya, I. and Yagishita, K. (2004) Suspended Cells from Trabecular Bone by Collagenase Digestion Become Virtually Identical to Mesenchymal Stem Cells Obtained from Marrow Aspirates. Blood, 104, 2728-2735.
http://dx.doi.org/10.1182/blood-2003-12-4452
[11] Miki, T., Lehmann, T., Cai, H., Stolz, D.B. and Strom, S.C. (2005) Stem Cell Characteristics of Amniotic Epithelial Cells. Stem Cells, 23, 1549-1559.
http://dx.doi.org/10.1634/stemcells.2004-0357
[12] Kim, J., Kang, H.M., Kim, H., Kim, M.R., Kwon, H.C. and Gye, M.C. (2007) Ex Vivo Characteristics of Human Amniotic Membrane-Derived Stem Cells. Cloning and Stem Cells, 9, 581-594.
http://dx.doi.org/10.1089/clo.2007.0027
[13] Portmann-Lanz, C.B., Schoeberlein, A., Huber, A., Sager, R., Malek, A. and Holzgreve, W. (2006) Placental Mesenchymal Stem Cells as Potential Autologous Graft for Pre- and Perinatal Neuroregeneration. American Journal of Obstetrics and Gynecology, 194, 664-673.
http://dx.doi.org/10.1016/j.ajog.2006.01.101
[14] Ilancheran, S., Michalska, A., Peh, G., Wallace, E.M., Pera, M. and Manuelpillai, U. (2007) Stem Cells Derived from Human Fetal Membranes Display Multilineage Differentiation Potential. Biology of Reproduction, 77, 577-588.
http://dx.doi.org/10.1095/biolreprod.106.055244
[15] Kamadjaja, D.B., Purwati, Ferdiansyah, Rantam, F.A. and Pramono, C. (2014) The Osteogenic Capacity of Human Amniotic Membrane Mesenchyma Stem Cell (hAMSC) and Potential for Applicationin Maxillofacial Bone Recon- struction in Vitro Study. Journal of Biomedical Science and Engineering, 7, 497-503.
http://dx.doi.org/10.4236/jbise.2014.78051
[16] Lindenmair, A., Hatlapatka, T., Kollwig, G., Hennerbichler, S., Gabriel, C., Wolbank, S., Redl, H. and Kasper, C. (2012) Mesenchymal Stem or Stromal Cells from Amnion and Umbilical Cord Tissue and Their Potential for Clinical Applications. Cells, 1, 1061-1088.
http://dx.doi.org/10.3390/cells1041061
[17] Shi, Y., Su, J., Roberts, A.I., Shou, P., Rabson, A.B. and Ren, G. (2012) How Mesenchymal Stem Cells Interact with Tissue Immune Responses. Trends in Immunology, 33, 136-143.
http://dx.doi.org/10.1016/j.it.2011.11.004
[18] Potier, E., Ferreira, E., Andriamanalijaona, R., Pujol, J.P., Oudina, K., Logeart-Avramoglou, D. and Petite, H. (2007) Hypoxia Affects Mesenchymal Stromal Cell Osteogenic Differentiation and Angiogenic Factor Expression. Bone, 40, 1078-1087.
http://dx.doi.org/10.1016/j.bone.2006.11.024
[19] Hanada, K., Solchaga, L.A., Caplan, A.I., Hering, T.M., Goldberg, V.M., Yoo, J.U. and Johnstone, B. (2001) BMP-2 Induction and TGF-Beta-1 Modulation of Rat Periosteal Cell Chondrogenesis. Journal of Cellular Biochemistry, 81, 284-294.
http://dx.doi.org/10.1002/1097-4644(20010501)81:2<284::AID-JCB1043>3.0.CO;2-D
[20] Wang, F.S., Yang, K.D., Kuo, Y.R., Wang, C.J., Sheen-Chen, S.M., Huang, H.C. and Chen, Y.J. (2003) Temporral and Spatial Expression of Bone Morphogenetic Proteins in Extracorporeal Shock Wave-Promoted Healing of Segmental Defect. Bone, 32, 387-396.
http://dx.doi.org/10.1016/S8756-3282(03)00029-2
[21] Schonmeyr, B.H., Soares, M., Avraham, T., Clavin, N.W., Gewalli, F. and Mehrara, B.J. (2010) Vascular Endothelial Growth Factor Inhibit Bone Morphogenetic Protein 2 Expression in Rat Mesenchymal Stem Cells. Tissue Engineering Part A, 16, 653-662.
http://dx.doi.org/10.1089/ten.tea.2009.0426
[22] Lin, Z.W., Wang, J.S., Lin, L.J., Zhang, J.W., Liu, Y.L., Ming, S. and Qi, L. (2014) Effects of BMP2 and VEGF165 on the Osteogenic Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells. Experimental and Therapeutic Medicine, 7, 625-629.
[23] Friedlaender, G. (1987) Current Concept Review, Bone Grafts: The Basic Science Rationale for Clinical Applications. The Journal of Bone & Joint Surgery, 69, 786-790.

Journals Menu
Follow SCIRP
Contact us

Copyright © 2021 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.