Use of Platelet-Rich Plasma for Collagen Matrixes Revitalization with Human Fibroblast


We studied in vitro attachment, proliferation and survival of сadaver skin fibroblasts in collagen bands, dermal matrix and cancellous demineralized bone, enriched with platelet components. It was shown, that PRP enhanced revitalization of collagen grafts, especially followed by components of activated or freezed platelets. Fibroblasts had the best rate of proliferative activity in response to 150 pg platelet derived growth factor (PDGF), released from 100 million platelets with granules and measured for 100 × 103 cultivated cells. The use of platelet-derived material could increase fibroblast proliferation activity for 1.5-3 times in all types of collagen transplants without damage or decay of cell’s biological value.

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Makarov, M. , Borovkova, N. , Konushko, O. and Khvatov, V. (2015) Use of Platelet-Rich Plasma for Collagen Matrixes Revitalization with Human Fibroblast. Journal of Biosciences and Medicines, 3, 80-87. doi: 10.4236/jbm.2015.310011.

Conflicts of Interest

The authors declare no conflicts of interest.


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