Protective Role of Golden Flaxseed (Linum usitatissimum L.) Against Oxidative Damage in Lipids and Proteins of Healthy Volunteers


Flaxseed (Linum usitatissimum L.) is composed mainly of bioactive components such as polyphenols, polyunsaturated fatty acids, fiber and lignans. Flaxseed can be found in different presentation forms (grain or flour) and varieties (brown or golden); however, questions have arisen as to whether the presentation form and/or variety may influence the health effects. The objective of this study was to evaluate the effects on blood pressure, anthropometric and oxidative parameters in healthy human volunteers. All subjects received 40 gram aliquots of flaxseed and were instructed to consume them in their entirety mixed with water in the morning for a period of 14 days. Oxidative parameters showed significant reductions (p < 0.05) in oxidative damage to lipids and proteins via dietary intervention with golden flaxseed grains. There were no significant differences in anthropometric parameters, blood pressure, DNA damage and micronuclei frequency after 14-day supplementation. This research indicates that golden flaxseed grains can be a valuable adjunct for disease prevention and protecting the organism against oxidative damage.

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Zuravski, L. , Coelho, R. , Duarte, J. , Rocha, M. , Mezzomo, J. , Pilar, B. , Galarça, L. , Athayde, M. , Boligon, A. , Machado, M. and Manfredini, V. (2015) Protective Role of Golden Flaxseed (Linum usitatissimum L.) Against Oxidative Damage in Lipids and Proteins of Healthy Volunteers. Journal of Biosciences and Medicines, 3, 45-53. doi: 10.4236/jbm.2015.310006.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Goyal, A., Sharma, V., Upadhyay, N., Gill, S. and Sihag, M. (2014) Flax and Flaxseed Oil: An Ancient Medicine & Modern Functional Food. Journal of Food Science and Technology, 51, 1633-1653.
[2] Chandra, S. and De Mejia Gonzalez, E. (2004) Polyphenolic Compounds, Antioxidant Capacity, and Quinone Reductase Activity of an Aqueous Extract of Ardisia compressa in Comparison to Mate (Ilex paraguariensis) and Green (Camellia sinensis) Teas. Journal of Agricultural and Food Chemistry, 52, 3583-3589.
[3] Woisky, R.G. and Salatino, A. (1998) Analysis of Propolis: Some Parameters and Procedures for Chemical Quality Control. Journal of Apicultural Research, 37, 99-105.
[4] Bloedon, L.T., Balikai, S., Chittams, J., Cunnane, S.C., Berlin, J.A., Rader, D.J., et al. (2008) Flaxseed and Cardiovascular Risk Factors: Results from a Double Blind, Randomized, Controlled Clinical Trial. Journal of the American College of Nutrition, 27, 65-74.
[5] Rajesha, J., Murthy, K.N., Kumar, M.K., Madhusudhan, B. and Ravishankar, G.A. (2006) Antioxidant Potentials of Flaxseed by in Vivo Model. Journal of Agricultural and Food Chemistry, 54, 3794-3799.
[6] Ohkawa, H., Ohishi, N. and Yagi, K. (1979) Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction. Analytical Biochemistry, 95, 351-358.
[7] Levine, R., Garland, D., Oliver, C., Amici, A., Climent, I., Lenz, A., et al. (1990) Damage to Proteins and Lipids Tissues under Oxidative Stress. Methods in Enzymology, 186, 464-478.
[8] Singh, N.P., McCoy, M.T., Tice, R.R. and Schneider, E.L. (1988) A Simple Technique for Quantitation of Low Levels of DNA Damage in Individual Cells. Experimental Cell Research, 175, 184-191.
[9] Schmid, W. (1975) The Micronucleus Test. Mutation Research, 31, 9-15.
[10] Aebi, H. (1984) Catalase in Vitro. Methods in Enzymology, 105, 121-126.
[11] Stahl, C.H., Novak, M., Lappas, G., Wilhelmsen, L., Bjorck, L., Hansson, P.O., et al. (2012) High-Normal Blood Pressure and Long-Term Risk of Type 2 Diabetes: 35-Year Prospective Population Based Cohort Study of Men. BMC Cardiovascular Disorders, 12, 89.
[12] Stuglin, C. and Prasad, K. (2005) Effect of Flaxseed Consumption on Blood Pressure, Serum Lipids, Hemopoietic System and Liver and Kidney Enzymes in Healthy Humans. Journal of Cardiovascular Pharmacology and Therapeutics, 10, 23-27.
[13] Furukawa, S., Fujita, T., Shimabukuro, M., Iwaki, M., Yamada, Y., Nakajima, Y., et al. (2004) Increased Oxidative Stress in Obesity and Its Impact on Metabolic Syndrome. Journal of Clinical Investigation, 114, 1752-1761.
[14] Rhee, Y. and Brunt, A. (2011) Flaxseed Supplementation Improved Insulin Resistance in Obese Glucose Intolerant People: A Randomized Crossover Design. Nutrition Journal, 10, 44.
[15] Tsang, A.H.K. and Chung, K.K.K. (2009) Oxidative and Nitrosative Stress in Parkinson’s Disease. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1792, 643-650.
[16] Prasad, K. (2005) Hypocholesterolemic and Antiatherosclerotic Effect of Flax Lignan Complex Isolated from Flaxseed. Atherosclerosis, 179, 269-275.
[17] Kasote, D. (2013) Flaxseed Phenolics as Natural Antioxidants. International Food Research Journal, 20, 27-34.
[18] Aladedunye, F., Sosinska, E. and Przybylski, R. (2013) Flaxseed Cyclolinopeptides: Analysis and Storage Stability. Journal of the American Oil Chemists’ Society, 90, 419-428.
[19] Abuzaytoun, R. and Shahidi, F. (2006) Oxidative Stability of Flax and Hemp Oils. Journal of the American Oil Chemists’ Society, 83, 855-861.
[20] Tamarit, J., de Hoogh, A., Obis, E., Alsina, D., Cabiscol, E. and Ros, J. (2012) Analysis of Oxidative Stress-Induced Protein Carbonylation Using Fluorescent Hydrazides. Journal of Proteomics, 75, 3778-3788.
[21] Chang, W.-H., Hu, S.-P., Huang, Y.-F., Yeh, T.-S. and Liu, J.-F. (2010) Effect of Purple Sweet Potato Leaves Consumption on Exercise-Induced Oxidative Stress and IL-6 and HSP72 Levels. Journal of Applied Physiology, 109, 1710-1715.
[22] Furness, D.L.F., Dekker, G.A. and Roberts, C.T. (2011) DNA Damage and Health in Pregnancy. Journal of Reproductive Immunology, 89, 153-162.
[23] Frieauff, W., Martus, H.J., Suter, W. and Elhajouji, A. (2013) Automatic Analysis of the Micronucleus Test in Primary Human Lymphocytes Using Image Analysis. Mutagenesis, 28, 15-24.
[24] Bub, A., Watzl, B., Blockhaus, M., Briviba, K., Liegibel, U., Müller, H., et al. (2003) Fruit Juice Consumption Modulates Antioxidative Status, Immune Status and DNA Damage. The Journal of Nutritional Biochemistry, 14, 90-98.
[25] Katz, D.L., Doughty, K. and Ali, A. (2011) Cocoa and Chocolate in Human Health and Disease. Antioxidants & Redox Signaling, 15, 2779-2811.
[26] Yuan, Y.V., Rickard, S.E. and Thompson, L.U. (1999) Short-Term Feeding of Flaxseed or Its Lignan Has Minor Influence on in Vivo Hepatic Antioxidant Status in Young Rats. Nutrition Research, 19, 1233-1243.
[27] Nelson, S.K., Bose, S.K., Grunwald, G.K., Myhill, P. and McCord, J.M. (2006) The Induction of Human Superoxide Dismutase and Catalase in Vivo: A Fundamentally New Approach to Antioxidant Therapy. Free Radical Biology & Medicine, 40, 341-347.

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