PCR-RFLP Methodology to Identify Nocardia Isolates in Cuba

Yaxsier de Armas; Carlos Manuel Fernández; Raúl Díaz; Nicolas Bioni Stefano; Gustavo Wissmann; Enrique José Calderón

doi:10.4236/aim.2015.510076

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AiM> Vol.5 No.10, September 2015

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PCR-RFLP Methodology to Identify Nocardia Isolates in Cuba ()

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DOI: 10.4236/aim.2015.510076 3,067 Downloads 3,536 Views Citations

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Yaxsier de Armas^1*, Carlos Manuel Fernández¹, Raúl Díaz¹, Nicolas Bioni Stefano², Gustavo Wissmann², Enrique José Calderón³

Affiliation(s)

¹Institute of Tropical Medicine, Pedro Kour, Havana, Cuba.
²Hospital de Clínicas de Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
³Department of Internal Medicine, Center of Biomedical Research Network in Epidemiology and Public Health, Virgen del Rocío University Hospital, Seville, Spain.

ABSTRACT

Nocardiosis diagnosis is a major challenge. The clinical features and radiological findings are nonspecific. Traditionally, Nocardia identification is based on colonial and microscopical morphology and biochemical tests. However, molecular biology techniques allow a better characterization of species and biotypes. PCR-RFLP of the 65-kDa heat shock protein (HSP) gene provides a rapid, sensitive, and time and labor-efficient method for this proposal. Using this technique, six of eight isolates tested were identified as Nocardia asteroides type VI. PCR-RFLP of the 65-kDa HSP gene could be very useful for determining the incidence of this pathogen in different population groups and its association with susceptibility/resistance profiles to the drugs of choice for treatment. This work is the first molecular detection of Nocardia species in Cuba.

KEYWORDS

Nocardia, PCR-RFLP, Cuba, Molecular Identification, 65-kDa HSP Gene

Cite this paper

Armas, Y. , Fernández, C. , Díaz, R. , Stefano, N. , Wissmann, G. and Calderón, E. (2015) PCR-RFLP Methodology to Identify Nocardia Isolates in Cuba. Advances in Microbiology, 5, 724-729. doi: 10.4236/aim.2015.510076.

Conflicts of Interest

The authors declare no conflicts of interest.

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