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Antioxidant Properties of Chinese Propolis in Ross Broilers Exposed to Heat Stress in Egypt

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DOI: 10.4236/ojvm.2015.59027    4,027 Downloads   4,983 Views   Citations

ABSTRACT

Detrimental effects of heat stress that causes great loss to poultry industry have grown the global attention for the importance of natural antioxidant in alleviating the oxidative stress symptoms in poultry. The antioxidant effect of Chinese ether extract propolis (EEP) on broilers (Ross 308) exposed to chronic heat stress has been evaluated. Eighty broilers (15 days old) were randomly allocated into five equal groups; each received a maintenance diet supplemented by vitamin C (250 mg/kg diet) or propolis (250, 500 and 750 mg/kg diet) or not supplemented (control) for 27 consecutive days. Blood samples were collected to analyze serum corticosteron, total antioxidant capacity (TAC) and lipid peroxidation activity (MDA) at the end of the experiment. Moreover, the active chemical components responsible for the biological activities of EEP have been determined using Gas Chromatography/Mass Spectrometry (GC/MS). Results revealed a significant decrease in corticosterone levels in broilers after vit C or EEP supplementation. This improvement is parallel to a significant increase in TAC and decrease in MDA in broilers after EEP supplementation, which may be due to its high flavonoid content. Results show that Chinese EEP has biologically active compounds responsible for excellent antioxidant activities that ameliorate the negative impacts of elevated temperature in summer season in Egypt.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Mahmoud, M. , Abdel-Mohsein, H. and Farghali, M. (2015) Antioxidant Properties of Chinese Propolis in Ross Broilers Exposed to Heat Stress in Egypt. Open Journal of Veterinary Medicine, 5, 197-209. doi: 10.4236/ojvm.2015.59027.

References

[1] Attia, Y., Al-Hanoun, A. and Bovera, F. (2011) Effect of Different Levels of Bee Pollen on Performance and Blood Profile of New Zealand White Bucks and Growth Performance of Their Offspring during Summer and Winter Months. Journal of Animal Physiology and Animal Nutrition, 95, 17-26.
[2] Gross, W.B. and Siegel, H.S.C. (1983) Evaluation of the Heterophil/Lymphocyte Ratio as a Measure of Stress in Chickens. Avian Diseases, 27, 972-979.
[3] Siegel, H.S. (1995) Stress, Strains and Resistance. British Poultry Science, 36, 3-22.
[4] Yegani, M. (2008) Summer and Heat Stress.
http://www.worldpoultry.net/Home/General/2008/6/Summer-and-heat-stress-WP003006W
[5] Quinteiro-Filho, W.M., Rodrigues, M.V., Ribeiro, A., Ferraz-de-Paula, V., Pinheiro, M.L., Sa, L.R., Ferreira, A.J. and Palermo-Neto, J. (2012) Acute Heat Stress Impairs Performance Parameters and Induces Mild Intestinal Enteritis in Broiler Chickens: Role of Acute HPA Axis Activation. Journal of Animal Science, 90, 1986-1994.
[6] Siegel, H.S. (1980) Physiological Stress in Birds. BioScience, 301, 529-533.
[7] Beuving, G., Jones, R.B. and Blokhuis, H.J. (1989) Adrenocortical and Heterophil/Lymphocyte Responses to Challenge in Hens Showing Short or Long Tonic Immobility Reactions. British Poultry Science, 30, 175-184.
[8] Ligeiro de Oliveira, A.P., Lazzarini, R., Cavriani, G., Quinteiro-Filho, W.M., Tavares de Lima, W. and Palermo-Neto, J. (2008) Effects of Single or Repeated Amphetamine Treatment and Withdrawal on Lung Allergic Inflammation in Rats. International Immunopharmacology, 8, 1164-1171.
[9] Hayashi, K., Nagai, Y., Ohtsuka, A. and Tomita, Y. (1994) Effects of Dietary Corticosterone and Trilostane on Growth and Skeletal Muscle Protein Turnover in Broiler Cockrels. British Poultry Science, 35, 789-798.
[10] Kumar, S., Ajeet, K. and Meena, K. (2011) Review Effect of Heat Stress in Tropical Livestock and Different Strategies For Its Amelioration. Journal of Stress Physiology & Biochemistry, 7, 45-54.
[11] Sies, H. (1991) Oxidative Stress: From Basic Research to Clinical Application. The American Journal of Medicine, 91, 31-38. http://dx.doi.org/10.1016/0002-9343(91)90281-2
[12] Nagai, T., Sakai, M., Inoue, R., Inoue, H. and Suzuki, N. (2001) Antioxidative of Some Commercially Honeys, Royal Jelly and Propolis. Food Chemistry, 75, 237-240.
http://dx.doi.org/10.1016/S0308-8146(01)00193-5
[13] Melo, E.A., Maciel, M.I.S., Lima, V.L.A.G., Leal, F.L.L., Caetano, A.C.S. and Nascimento, R.J. (2006) Capacidade antioxidante de hortalicas usualmente consumidas. Ciência e Tecnologia de Alimentos, 26, 639-644.
http://dx.doi.org/10.1590/S0101-20612006000300024
[14] Ardestani, A. and Yazdanparast, R. (2007) Antioxidant and Free Radical Scavenging Potential of Achillea santolina Extracts. Food Chemistry, 104, 21-29.
http://dx.doi.org/10.1016/j.foodchem.2006.10.066
[15] Sharififar, F., Moshafi, M.H., Mansouri, S.H., Khoshademas, M. and Koshinoodi, M. (2006) In Vitro Avaluation of Antibacterial and Antioxidant Activities of the Essential Oil and Methanol Extracts of Endemic Zataria multiflora Boiss. Food Control, 18, 800-805.
http://dx.doi.org/10.1016/j.foodcont.2006.04.002
[16] Tatli Seven, P., Yilmaz, S., Seven, I., Cerci, I.H., Azman, M.A. and Yilmaz, M. (2009) Effect of Propolis on Selected Blood Indicators and Antioxidant Enzyme Activities in Broilers under Heat Stress. Acta Veterinaria Brno, 78, 75-83.
http://dx.doi.org/10.2754/avb200978010075
[17] Sahin, N., Onderci, M., Sahin, K., Gursu, M.F. and Smith, M.O. (2004) Ascorbic Acid and Melatonin Reduces Heat Induced Performance Inhibition and Oxidative Stress in Japanese Quails. British Poultry Science, 45, 116-122.
http://dx.doi.org/10.1080/00071660410001668941
[18] Halliwell, B. (1991) Reactive Oxygen Species in Living Systems: Source, Biochemistry, and Role in Human Disease. The American Journal of Medicine, 91, 14-22.
http://dx.doi.org/10.1016/0002-9343(91)90279-7
[19] Tatli Seven, P., Yilmaz, S., Seven, I. and Tuna Kelestemur, G. (2012) The Effects of Propolis in Animals Exposed Oxidative Stress. In: Lushchak, V.I., Ed., Oxidative Stress—Environmental Induction and Dietary Antioxidants, Chap. 13, InTECH BOOK, Rijeka, 267-288.
[20] Tatli Seven, P., Seven, I., Yilmaz, M. and Simsek, G. (2008) The Effects of Turkish Propolis on Growth and Carcass Characteristics in Broilers under Heat Stress. Animal Feed Science and Technology, 146, 137-148.
http://dx.doi.org/10.1016/j.anifeedsci.2007.11.003
[21] Seven, I., Aksu, T. and Seven, P.T. (2010) The Effects of Propolis on Biochemical Parameters and Activity of Antioxidant Enzymes in Broilers Exposed to Lead-Induced Oxidative Stress. Journal of Animal Science, 23, 1482-1489.
[22] Varaprasad Reddy, L.S.S., Leela, V., Sudhakara Reddy, B. and Ananda Reddy, P. (2014) A Study of the Effect of Vitamin C and Ocimum sanctum Supplementation on Antioxidant Enzyme Levels in Broilers under Heat-Stress. International Journal of Veterinary Health Science & Research, 2, 21-23.
[23] Choi, J.Y., Pena, I.C., Yoon, S.Y., Woo, T.S, Choi, Y.J., Shin, C.Y., Ryn, J.H., Lee, Y.S., Yu, G.Y. and Cheong, J.H. (2011) Is the Anti-Stress Effect of Vitamin C Related to Adrenal Gland Function in Rat. Food Science and Biotechnology, 20, 429-435.
http://dx.doi.org/10.1007/s10068-011-0060-3
[24] Mckee, J.S. and Hurrison, P.C. (1995) Effects of Supplemental Ascorbic Acid on the Performance of Broiler Chickens Exposed to Multiple Concurrent Stressors. Poultry Science, 74, 1772-1785.
http://dx.doi.org/10.3382/ps.0741772
[25] Kumaran, A. and Karakumaran, J. (2007) In Vitro Antioxidant Activities of Methanol Extracts of Five Phyllanthus Species from India. LWT—Food Science and Technology, 40, 344-352.
http://dx.doi.org/10.1016/j.lwt.2005.09.011
[26] Mosquera, O.M., Correra, Y.M. and Nino, J. (2009) Antioxidant Activity of Plant Extracts from Colombian Flora. Revista Brasileira de Farmacognosia, 19, 382-387.
http://dx.doi.org/10.1590/S0102-695X2009000300008
[27] Valle, M.L. (2000) Quantitative Determination of Antibacterian Capacities of Propolis. Apiacta, 35, 152-161.
[28] Türkez, H., Yousef, M.I. and Geyikoglu, F. (2010) Propolis Prevents Aluminum-Induced Genetic and Hepatic Damages in Rat Liver. Food and Chemical Toxicology, 48, 2741-2746.
http://dx.doi.org/10.1016/j.fct.2010.06.049
[29] Khalil, M.L. (2006) Biological Activity of Bee Propolis in Health and Disease. Asian Pacific Journal of Cancer Prevention, 7, 22-31.
[30] Mohammadzadeh, S., Shariatpanahi, M., Hamedi, M., Ahmadkhaniha, M., Samadi, R. and Ostad, S.N. (2007) Chemical Composition, Oral Toxicity and Antimicrobial Activity of Iranian Propolis. Food Chemistry, 103, 1097-1103.
http://dx.doi.org/10.1016/j.foodchem.2006.10.006
[31] Amoros, M., Simoes, C.M.O., Girre, L., Sauvager, F. and Cormier, M. (1992) Synergistic Effect of Flavones and Flavonols against Herpes Simplex Virus Type I in Cell Culture Comparison with the Antiviral Activity of Propolis. Journal of Natural Products, 55, 1732-1740.
http://dx.doi.org/10.1021/np50090a003
[32] Dobrowolski, J.W., Vohoraq, S.B., Sharma, K., Shah, S.A., Naqvi, S.A.H. and Dandiya, P.C. (1991) Antibacterial, Antifungal, Antiamoebic, Antiinflammatory, and Antipyretic Studies on Propolis Bee Products. Journal of Ethnopharmacology, 35, 77-82.
http://dx.doi.org/10.1016/0378-8741(91)90135-Z
[33] Banskota, A.H., Nagaoka, T., Sumioka, L.Y., Tezuka, Y., Awale, S., Midorikawa, K., Matsushige, K. and Kadota, S. (2002) Antiproliferative Activity of the Netherlands Propolis and Its Active Principles in Cancer Cells Lines. Journal of Ethnopharmacology, 80, 67-73.
http://dx.doi.org/10.1016/s0378-8741(02)00022-3
[34] Missima, F. and Sforcin, J.M. (2008) Green Brazilian Propolis Action on Macrophages and Lymphoid Organs of Chronically Stressed Mice. Evidence-Based Complementary and Alternative Medicine, 5, 71-75.
http://dx.doi.org/10.1093/ecam/nel112
[35] Marcucci, M.C. (1996) Propriedades biológicas e terapêuticas dos constituintes químicos da própolis. Quimica Nova, 19, 529-536.
[36] Ahn, M., Kumazawa, S., Usui, Y., Nakamura, J., Matsuka, M., Zhu, F. and Nakayama, T. (2007) Antioxidant Activity and Constituents of Propolis Collected in Various Areas of China. Food Chemistry, 101, 1400-1409.
http://dx.doi.org/10.1016/j.foodchem.2006.03.045
[37] Da Silva, J.F.M., Souza, M.C., Matta, S.R., Andrade, M.R. and Vidal, F.V.N. (2006) Correlation Analysis between Phenolic Levels of Brazilian Propolis Extracts and Their Antimicrobial and Antioxidant Activities. Food Chemistry, 99, 431-435.
http://dx.doi.org/10.1016/j.foodchem.2005.07.055
[38] Choi, Y.M., Noh, D.O., Cho, S.Y., Suh, H.J., Kim, K.M. and Kim, J.M. (2006) Antioxidant and Antimicrobial Activities of Propolis from Several Regions of Korea. LWT—Food Science and Technology, 39, 756-761.
http://dx.doi.org/10.1016/j.lwt.2005.05.015
[39] Basnet, P., Matsuno, T. and Neidlein, R. (1997) Potent Free Radical Scavenging Activity of Propol Isolated from Brazilian Propolis. Zeitschrift für Naturforschung, 52c, 828-833.
[40] Banskota, A.H., Tezuka, T., Adyana, I.K., Midorikawa, K., Matsushige, K., Message, D., Huertas, A.G.A. and Kadota, S. (2000) Cytotoxic, Hepatoprotective and Free Radical Scavenging Effects of Propolis from Brazil, Peru, the Netherlandas and China. Journal of Ethnopharmacology, 72, 239-246.
http://dx.doi.org/10.1016/S0378-8741(00)00252-X
[41] Havsteen, B.H. (2002) The Biochemistry and Medical Significance of the Flavonoids. Pharmacology & Therapeutics, 96, 67-202.
http://dx.doi.org/10.1016/S0163-7258(02)00298-X
[42] Tatli Seven, P. (2008) The Effects of Dietary Turkish Propolis and Vitamin C on Performance, Digestibility, Egg Production and Egg Quality in Laying Hens under Different Environmental Temperatures. Asian-Australasian Journal of Animal Sciences, 21, 1164-1170.
http://dx.doi.org/10.5713/ajas.2008.70605
[43] Seven, I., Tatli Seven, P. and Silici, S. (2011) Effects of Dietary Turkish Propolis as Alternative to Antibiotic on Growth and Laying Performances, Nutrient Digestibility and Egg Quality in Laying Hens under Heat Stress. Revue de Médecine Vétérinaire, 162, 186-191.
[44] Ohkawa, H., Ohishi, N. and Yagi, K. (1979) Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction. Analytical Biochemistry, 95, 351-358.
http://dx.doi.org/10.1016/0003-2697(79)90738-3
[45] Kumazawa, S., Hamasaka, T. and Nakayama, T. (2004) Antioxidant Activity of Propolis of Various Geographic Origins. Food Chemistry, 84, 329-339.
http://dx.doi.org/10.1016/S0308-8146(03)00216-4
[46] NRP (1994) Nutrient Requirements of Poultry. Ninth Revised Edition, The National Academies of Sciences, Engineering, and Medicine, Washington DC.
http://www.nap.edu/openbook.php?isbn=0309048923
[47] Bankova, V., Popova, M., Bogdanov, S. and Sabatini, A.G. (2002) Chemical Composition of European Propolis: Expected and Unexpected Results. Zeitschrift für Naturforschung, 57c, 530-533.
[48] Garriga, C., Hunter, R.R., Amat, C., Planas, J.M., Mitchell, M.A. and Moreto, M. (2006) Heat Stress Increases Apical Glucose Transport in the Chicken Jejunum. American Journal of Physiology—Regulatory, Integrative and Comparative Physiology, 290, R195-R201.
http://dx.doi.org/10.1152/ajpregu.00393.2005
[49] Astolfi-Ferreira, C.S., Ferreira, A.J. and Palermo-Neto, J. (2012) Heat Stress Impairs Performance and Induces Intestinal Inflammation in Broiler Chickens Infected with Salmonella Enteritidis. Avian Pathology, 41, 421-427.
http://dx.doi.org/10.1080/03079457.2012.709315
[50] Kutlu, H.R. and Forbes, J.M. (1993) Changes in Growth and Blood Parameters in Heat Stressed Broiler Chicks in Response to Dietary Ascorbic Acid. Livestock Production Science, 36, 335-350.
http://dx.doi.org/10.1016/0301-6226(93)90050-R
[51] Mahmoud, U.T., Abdel-Rahman, M.A.M. and Darwish, M.H.A. (2014) Effects of Propolis, Ascorbic Acid and Vitamin E on Thyroid and Corticosterone Hormones in Heat Stressed Broilers. Journal of Advanced Veterinary Research, 4, 18-27.
[52] Quinteiro-Filho, W.M., Ribeiro, A., Ferraz-de-Paula, V., Pinheiro, M.L., Sakai, M., Sa, L.R., Ferreira, A.J. and Palermo-Neto, J. (2010) Heat Stress Impairs Performance Parameters, Induces Intestinal Injury, and Decreases Macrophage Activity in Broiler Chickens. Poultry Science, 89, 1905-1914.
http://dx.doi.org/10.3382/ps.2010-00812
[53] Sujatha, V., Korde, J.P., Rastogi, S.K., Maini, S., Ravikanth, K. and Rekhe, D.S. (2010) Amelioration of Heat Stress Induced Disturbances of the Antioxidant Defense System in Broilers. Journal of Veterinary Medicine and Animal Health, 2, 18-28.
[54] Nockels, C.F., Lopez, G.A. and Phillips, R.W. (1973) Influence of Vitamin A and C on Corticosterone and Carbohydrate Metabolism in Chickens. Poultry Science, 52, 1261-1269.
http://dx.doi.org/10.3382/ps.0521261
[55] Sheila, K.S. and Cheryl, F.N. (1978) Effect of Age, Sex, and Ascorbic Acid Ingestion on Chicken Plasma Corticosterone Levels. Poultry Science, 57, 527-533.
http://dx.doi.org/10.3382/ps.0570527
[56] Mckee, J.S., Harrison, P.C. and Riskowski, G.L. (1997) Effect of Supplemental Ascorbic Acid on the Energy Conversion of Broiler Chicks during Heat Stress and Feed Withdrawal. Poultry Science, 76, 1278-1286.
http://dx.doi.org/10.1093/ps/76.9.1278
[57] Pagliarone, A.C., Orsatti, C.L., Búfalo, M.C., Missima, F., Bachiega, T.F., Araújo Jr., J.P. and Sforcin, J.M. (2009) Propolis Effects on Pro-Inflammatory Cytokine Production and Toll-Like Receptor 2 and 4 Expression in Stressed Mice. International Immunopharmacology, 9, 1352-1356.
http://dx.doi.org/10.1016/j.intimp.2009.08.005
[58] Orsatti, C.L. and Sforcin, J.M. (2012) Propolis Immunomodulatory Activity on TLR-2 and TLR-4 Expression by Chronically Stressed Mice. Natural Product Research, 26, 446-453.
http://dx.doi.org/10.1080/14786419.2010.482049
[59] Nakamura, T., Ohta, Y., Tada, M., Teruya, A., Ohashi, K., Ikeno, K., et al. (2011) Protective Effect of Brazilian Propolis Ethanol Extract against Stress Induced Gastric Mucosal Lesions in Rats. Its Evaluation Using Oxidative Stress Markers. Journal of Analytical Bio-Science, 34, 135-146.
[60] Lee, M., Kim, Y.H., Park, W., Ahn, W.G., Park, O.K., Kwon, S., Morita, K., Shim, I. and Her, S. (2013) Novel Antidepressant-Like Activity of Propolis Extract Mediated by Enhanced Glucocorticoid Receptor Function in the Hippocampus. Evidence-Based Complementary and Alternative Medicine, 2013, 1-10.
[61] Selem, A.S.M. (2012) Effect of Propolis on Ruminal Fermentation, Reproductive and Productive Performance of Santa Ines Ewes. PhD Thesis, Center of Nuclear Energy and Agriculture, San Poulo University, Piracicaba.
[62] Ohno, S., Satoshi, S., Satoshi, T., Hiroyuki, N, Tsunehisa, M. and Shizuo, N. (2002) Effect of Flavonoid Phytochemical on Cortisol Production and on Activities of Steroidogenic Enzyme in Human Adrenocortical H295R Cells. Journal of Steroid Biochemistry and Molecular Biology, 80, 355-363. http://dx.doi.org/10.1016/S0960-0760(02)00021-3
[63] Li, Y.J., Xuan, H.Z., Shou, Q.Y., Zhan, Z.G., Lu, X., and Hu, F.L. (2012) Therapeutic Effects of Propolis Essential Oil on Anxiety of Restraint-Stressed Mice. Human and Experimental Toxicology, 31, 157-165.
http://dx.doi.org/10.1177/0960327111412805
[64] Abdel-Mohsein, H.S., Mahmoud, M.A.M. and Mahmoud, U.T. (2014) Influence of Propolis on Intestinal Microflora of Ross Broilers Exposed to Hot Environment. Advances in Animal and Veterinary Sciences, 2, 204-211.
http://dx.doi.org/10.14737/journal.aavs/2014/2.4.204.211
[65] Temple, N.J. (2000) Antioxidants and Disease: More Questions than Answers. Nutrition Research, 20, 449-459.
http://dx.doi.org/10.1016/S0271-5317(00)00138-X
[66] Frey, B. (1991) Vitamin C Protects Lipids in Human Plasma and Low Density Lipoprotein against Oxidative Damage. The American Journal of Clinical Nutrition, 54, 113.
[67] Agudelo, L.G. (1983) Possible Causes of Avian Oedema. International Journal of Poultry Sciences, 22, 8-14.
[68] Lutsenko, E.A., Carcamo, J.M. and Golde, D.W. (2002) Vitamin C Prevents DNA Mutation Induced by Oxidative Stress. The Journal of Biological Chemistry, 277, 16895-16899.
http://dx.doi.org/10.1074/jbc.M201151200
[69] Hashem, N.M., AbdEl-Hady, A. and Hassan, O. (2013) Effect of Vitamin E or Propolis Supplementation on Semen Quality, Oxidative Status and Hemato-Biochemical Changes of Rabbit Bucks during Hot Season. Livestock Science, 157, 520-526.
http://dx.doi.org/10.1016/j.livsci.2013.09.003
[70] Nagaia, T., Inoueb, R., Inoueb, H. and Suzuki, N. (2003) Preparation and Antioxidant Properties of Water Extract of Propolis. Food Chemistry, 80, 29-33.
http://dx.doi.org/10.1016/S0308-8146(02)00231-5
[71] De Sá, R.A., de Castro, F.A.V., Eleutherio, E.C.A., de Souza, R.M., da Silva, J.F.M. and Pereira, M.D. (2013) Brazilian Propolis Protects Saccharomyces cerevisiae Cells against Oxidative Stress. Brazilian Journal of Microbiology, 44, 993-1000.
http://dx.doi.org/10.1590/S1517-83822013005000062
[72] Fabris, S., Bertelle, M., Astafyeva, O., Gregoris, E., Zangrando, R., Gambaro, A., Lima, G.P.P. and Stevanato, R. (2013) Antioxidant Properties and Chemical Composition Relationship of Europeans and Brazilians Propolis. Pharmacology & Pharmacy, 4, 46-51.
http://dx.doi.org/10.4236/pp.2013.41006
[73] Christov, R., Trusheva, B., Popova, M., Bankova, V. and Bertrand, M. (2006) Chemical Composition of Propolis from Canada, Its Antiradical Activity and Plant Origin. Natural Product Research, 20, 531-536.
http://dx.doi.org/10.1080/14786410500056918
[74] Gardana, C., Scaglianti, M., Pietta, P. and Simonetti, P. (2007) Analysis of the Polyphenolic Fraction of Propolis from Different Sources by Liquid Chromatography-Tandem Mass Spectrometry. Pharmaceutical and Biomedical Analysis, 45, 390-399.
http://dx.doi.org/10.1016/j.jpba.2007.06.022
[75] Fujimoto, T., Nakamura, J. and Matsuka, M. (2001) Diversity of Propolis. Part 1. Propolis from the World. Honeybee Science, 22, 9-16. (In Japanese)
[76] Bankova, V. (2005) Recent Trends and Important Developments in Propolis Research. Evidence-Based Complementary and Alternative Medicine, 2, 29-32.
http://dx.doi.org/10.1093/ecam/neh059
[77] Bonvehi, J.S. and Coll, F.V. (1994) Phenolic Composition of Propolis from China and from South America. Zeitschrift Fur Naturforschung C-A Journal of Biosciences, 49, 712-718.
[78] Isla, M.I., Nieva, M.I., Sampietro, A.R. and Vattuone, M.A. (2001) Antioxidant Activity of Argentine Propolis Extracts. Journal of Ethnopharmacology, 76, 165-170.
http://dx.doi.org/10.1016/S0378-8741(01)00231-8
[79] Volpi, N. and Bergonzini, G. (2006) Analysis of Flavonoids from Propolis by On-Line HPLC-Electrospray Mass Spectrometry. Pharmaceutical and Biomedical Analysis, 42, 354-361.
http://dx.doi.org/10.1016/j.jpba.2006.04.017
[80] Guo, X., Chen, B., Luo, L., Zhang, X., Dai, X. and Gong, S. (2011) Chemical Compositions and Antioxidant Activities of Water Extracts of Chinese Propolis. Journal of Agricultural and Food Chemistry, 59, 12610-12616.
http://dx.doi.org/10.1021/jf202818p
[81] Gregoris, E. and Stevanato, R. (2010) Correlations between Polyphenolic Composition and Antioxidant Activity of Venetian Propolis. Food and Chemical Toxicology, 48, 76-82.
http://dx.doi.org/10.1016/j.fct.2009.09.018
[82] El Sohaimy, S.A. and Masry, S.H.D. (2014) Antioxidant and Antimicrobial Activities of Egyptian and Chinese Propolis. American-Eurasian Journal of Agricultural & Environmental Sciences, 14, 1116-1124.
[83] Halliwell, B. and Gutteridge, J. (1998) Free Radicals in Biology and Medicine. Oxford University Press, Oxford.
[84] Mishra, A., Kumar, S. and Pandey, A.K. (2013) Scientific Validation of the Medicinal Efficacy of Tinospora cordifolia. The Scientific World Journal, 2013, Article ID: 292934.
http://dx.doi.org/10.1155/2013/292934
[85] Kumar, S. and Pandey, A.K. (2013) Chemistry and Biological Activities of Flavonoids: An Overview. The Scientific World Journal, 2013, Article ID: 162750.
http://dx.doi.org/10.1155/2013/162750
[86] Papotti, G., Bertelli, D., Bortolotti, L. and Plessi, M. (2012) Chemical and Functional Characterization of Italian Propolis Obtained by Different Harvesting Methods. Journal of Agricultural and Food Chemistry, 60, 2852-2862.
http://dx.doi.org/10.1021/jf205179d
[87] Boufadi, Y.M., Soubhye, J., Riazi, A., Rousseau, A., Vanhaeverbeek, M., Nève, J., Boudjeltia, K.Z. and Van Antwerpen, P. (2014) Characterization and Antioxidant Properties of Six Algerian Propolis Extracts: Ethyl Acetate Extracts Inhibit Myeloperoxidase Activity. International Journal of Molecular Sciences, 15, 2327-2345.
http://dx.doi.org/10.3390/ijms15022327
[88] Fonseca, Y.M., Marquele-Oliveira, F., Vicentini, F.T.M.C., Furtado, N.A.J.C., Sousa, J.P.B., Lucisano-Valim, Y.M. and Fonseca, M.J.V. (2011) Evaluation of the Potential of Brazilian Propolis against UV-Induced Oxidative Stress. Evidence-Based Complementary and Alternative Medicine, 2011, Article ID: 863917.
http://dx.doi.org/10.1155/2011/863917
[89] Bankova, V.S., De Castro, S.L. and Marcucci, M.C. (2000) Propolis: Recent Advances in Chemistry and Plant Origin. Apidologie, 31, 3-15.
http://dx.doi.org/10.1051/apido:2000102
[90] Savickas, A., Majiene, D., Ramanauskiene, K., Pavilonis, A., Muselik, J., Masteikova, R., et al. (2005) Chemical Composition and Antimicrobial Activity of Lithuanian and Czech Propolis. Biologija, 4, 59-63.
[91] Chen, Y.W., Wu, S.W., Ho, K.K., Lin, S.B., Huang, C.Y. and Chen, C.N. (2008) Characterization of Taiwanese Propolis Collected from Different Locations and Seasons. Journal of the Science of Food and Agriculture, 88, 412-419.
http://dx.doi.org/10.1002/jsfa.3101

  
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