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Diagnosis of Rabies via RT-PCR on Skin Samples of Wild and Domestic Animals

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DOI: 10.4236/ojvm.2015.59026    3,606 Downloads   4,233 Views   Citations
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ABSTRACT

In developing countries, brain tissues from rabies suspect animals are not always available for diagnosis for a variety of reasons, such as lack of transport to submit a carcass or the difficulty of removing an animal’s head or brain under field conditions. To enable diagnosis in such cases, there is a need for a reliable method, using an alternative non-neural tissue, which can be removed and submitted to the diagnostic laboratory without special training or equipment. In human medicine, skin is used successfully for the detection of rabies virus antigen using RT-PCR technology. Little work has been done in animals using RT-PCR on skin or extracted hair follicles. The current study was conducted in Grenada on skin from 36 wild and domestic animals, in which rabies virus infection had been confirmed in brain tissue via the direct fluorescent antibody (DFA) test, and in 31 negative control animals. RT-PCR on skin yielded a sensitivity of 97.2% (35/36) and a specificity of 100% (31/31). It is concluded that the examination of skin samples via RT-PCR provides a valuable diagnostic alternative in those cases where brain tissue is not readily available.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Zieger, U. (2015) Diagnosis of Rabies via RT-PCR on Skin Samples of Wild and Domestic Animals. Open Journal of Veterinary Medicine, 5, 191-196. doi: 10.4236/ojvm.2015.59026.

References

[1] Charlton, K.M. (1988) The Pathogenesis of Rabies. In: Campbell, J.M. and Charlton, K.M., Eds., Rabies. Kluwer Academic Publishers, Boston, 101-150.
http://dx.doi.org/10.1007/978-1-4613-1755-5_5
[2] OIE-World Organization for Animal Health (2015) Manual of Diagnostic Tests and Vaccines for Terrestrial Animals: Chapter 2.01.13 Rabies (Version Adopted in May 2013).
http://www.oie.int/manual-of-diagnostic-tests-and-vaccines-for-terrestrial-animals/
[3] World Health Organization (WHO) (2015) WHO Expert Consultation on Rabies. WHO Press, Geneva.
http://www.who.int/rabies/home_diagnosis/en
[4] Everard, C.O.R., James, A.C. and DaBreo, S. (1979) Ten Years of Rabies Surveillance in Grenada. Bulletin of the Pan American Health Organization, 13, 342-353.
[5] Everard, C.O.R. and Everard, J.D. (1992) Mongoose Rabies in the Caribbean. Annals of the New York Academy of Sci- ences, 653, 356-366.
http://dx.doi.org/10.1111/j.1749-6632.1992.tb19662.x
[6] Jonkers, A.H., Alexis, F. and Loregnard, R. (1969) Mongoose Rabies in Grenada. West Indian Medical Journal, 18, 167-170.
[7] Zieger, U., Marston, D.A., Sharma, R., Chikweto, A., Tiwari, K., Sayyid, M., Louison, B., Goharrriz, K., Breed, A.C., Werling, D., Fooks, A.R. and Horton, D.L. (2014) The Phylogeography of Rabies in Grenada, West Indies, and Prospects for Control. PLoS Neglected Tropical Diseases, 8, Article ID: e3251.
http://dx.doi.org/10.1371/journal.pntd.0003251
[8] Umoh, J.U. and Blenden, D.C. (1982) The Dissemination of Rabies Virus into Cranial Nerves and Other Tissues of Experimentally Infected Goats and Dogs, and Naturally Infected Skunks. International Journal of Zoonoses, 9, 1-11.
[9] Rupprecht, C.E., Hanlon, C.A. and Hemachudha, T. (2002) Rabies Re-Examined. Lancet, 2, 327-343.
http://dx.doi.org/10.1016/S1473-3099(02)00287-6
[10] Trimarchi, C.V. and Nadin-Davis, S.A. (2007) Diagnostic Evaluation. In: Jackson, A.C. andWunner, W.H., Eds., 2nd Edition, Rabies, Academic Press, San Diego, 411-470.
http://dx.doi.org/10.1016/B978-012369366-2/50012-9
[11] Blenden, D.C., Bell, J.F., Tsao, A.T. and Umoh, J.U. (1983) Immunofluorescent Examination of the Skin of Rabies- Infected Animals as a Means of Early Detection of Rabies Virus Antigen. Journal of Clinical Microbiology, 18, 631- 636.
[12] Dacheux, L., Reynes, J.M., Buchy, P., Sivuth, O., Diop, B.M., Rousset, D., Rathat, C., Jolly, N., Dufourcq, J.B., Nareth, C., Diop, S., Iehle, C., Rajerison, R., Sadorge, C. and Bourhy, H. (2008) A Reliable Diagnosis of Human Rabies Based on Analysis of Skin Biopsy Specimens. Clinical Infectious Diseases, 47, 1410-1417.
http://dx.doi.org/10.1086/592969
[13] Wacharapluesadee, S., Tepsumethanon, V., Supavonwong, P., Kaewpom, T., Intarut, N. and Hemachudha, T. (2012) Detection of Rabies Viral RNA by TaqMan Real-Time RT-PCR Using Non-Neural Specimens from Dogs Infected with Rabies Virus. Journal of Virological Methods, 184, 109-112.
http://dx.doi.org/10.1016/j.jviromet.2012.05.013
[14] Bansal, K., Singh, C.K.K., Ramneek, Sandhu, B.S., Deka, D., Dandale, M. and Sood, N.K. (2012) Antemortem Diagnosis of Rabies from Skin: Comparison of Nested RT-PCR with TaqMan Real Time PCR. Brazilian Journal of Veterinary Pathology, 5, 116-119.
[15] Bansal, K., Singh, C.K., Sandhu, B.S., Sood, N.K. and Dandale, M. (2014) Antemortem Diagnosis of Rabies from Skin by TaqMan Real Time PCR. Indian Journal of Animal Research, 48, 597-600.
http://dx.doi.org/10.5958/0976-0555.2014.00038.7
[16] Kaw, A., Singh, C.K., Ramneek, Deka, D., Sandhu, B.S., Awahan, S. and Sood, N.K. (2014). Comparison of Hair Follicles and Skin for Ante Mortem Detection of Rabies: A Molecular Approach. Indian Journal of Public Health Research and Development, 5, 165-169.
http://dx.doi.org/10.5958/0976-5506.2014.00296.4
[17] Bryceson, A.D.M., Greenwood, B.M., Warrell, D.A., Davidson, N.M., Pope, H.M., Lawrie, J.H, Barnes, H.J., Ballie, W.E. and Wilcox, G.E. (1975) Demonstration during Life of Rabies Antigen in Humans. Journal of Infectious Diseases, 131, 71-74.
http://dx.doi.org/10.1093/infdis/131.1.71
[18] Crepin, P., Audry, L., Rotivel, Y., Gacoin, A., Caroff, C. and Bourhy, H. (1998) Intravitam Diagnosis of Human Rabies by PCR Using Saliva and Cerebrospinal Fluid. Journal of Clinical Microbiology, 36, 1117-1121.
[19] Warrel, M.J., Looareesuwan, S., Manatsathit, S., White, N.J., Phuapradit, P., Vejjajiva, A., Hoke, C.H., Burke, D.S. and Warrell, D.A. (1988) Rapid Diagnosis of Rabies and Post-Vaccinalencephalitides. Clinical and Experimental Immunology, 71, 229-234.
[20] Smith, W.B., Blenden, D.C., Fuh, T.H. and Hiler, L. (1972) Diagnosis of Rabies by Immunofluorescent Staining of Frozen Sections of Skin. Journal of the American Veterinary Medical Association, 161, 1495-1501.
[21] Brito, M.G., Chamone, T.L., da Silva, F.J., Wada, M.Y., de Miranda, A.B., Castilho, J.G., Carrieri, M.L., Kotait, I. and Lemos, F.L. (2011) Antemortem Diagnosis of Human Rabies in a Veterinarian Infected When Handling a Herbivore in Minas Gerais, Brazil. Revista do Instituto de Medicina Tropical de Sao Paulo, 53, 39-44.
http://dx.doi.org/10.1590/s0036-46652011000100007
[22] Macedo, C.I., Carnieli Jr., P., Brandao, P.E., Travassos da Rosa, E.S., de Novaes-Oliveira, R., Galera-Castilho, J., Medeiros, R., Rocha-Machado, R., de Olivera, R.C., Carrieri, M.L. and Kotait, I. (2006) Diagnosis of Human Rabies Cases by Polymerase Chain Reaction of Neck-Skin Samples. The Brazilian Journal of Infectious Diseases, 10, 341-345.
http://dx.doi.org/10.1590/S1413-86702006000500008
[23] Smith, J., McElhinney, L., Parsons, G., Brink, N., Doherty, T., Agranoff, D., Miranda, M.E. and Fooks, A.R. (2003) Case Report: Rapid Ante-Mortem Diagnosis of a Human Case of Rabies Imported into the UK from the Philippines. Journal of Medical Virology, 69, 150-155.
http://dx.doi.org/10.1002/jmv.10253
[24] Blenden, D.C., Frost, J.W., Wachendoerfer, G. and Dorsey, C. (1980) Identification of Rabies Virus Antigen in the Skin of Foxes. Zentralblatt für Veterinarmedizin Reihe B, 27, 698-704.
http://dx.doi.org/10.1111/j.1439-0450.1980.tb02024.x
[25] Blenden, D.C. (1981) Rabies in a Litter of Skunks Predicted and Diagnosed by Skin Biopsy. Journal of the American Veterinary Medical Association, 179, 789-791.
[26] Ciuchini, F., Pestalozza, S., Buonavoglia, C. and Irsara, A. (1984) Evaluation of Immunofluorescence Staining of Skin for the Diagnosis of Rabies. Zentralblatt für Veterinarmedizin Reihe B, 31, 32-35.
http://dx.doi.org/10.1111/j.1439-0450.1984.tb01277.x
[27] Wacharapluesadee, S. and Hemachudha, T. (2010) Ante- and Post-Mortem Diagnosis of Rabies Using Nucleic Acid Amplification Tests. Expert Review of Molecular Diagnostics, 10, 207-218.
http://dx.doi.org/10.1586/erm.09.85

  
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