Occurrence of Biologically Inactive Corrinoid Compounds in Canned Edible Apple Snails (Escargots)


In this study, we characterized and quantified vitamin B12 in canned apple snails, escargots, (boiled plain) using a microbiological assay based on Lactobacillus delbrueckii ATCC 7830. Vitamin B12 contents of canned escargots (boiled plain) were varied from approximately 0.8 μg/100g weight to approximately 5.5 μg/100g weight (mean values, 2.2 μg/100g weight). We identified vitamin B12 compounds from escargots using liquid chromatography-electrospray ionization/tandem mass spectrometry. We found that escargots contained true vitamin B12 and two inactive corrinoids, which were identified as factor IIIm (or methoxymensimidazolyl cyanocobamide), and factor S (or 2-methylmercaptoadenyl cyanocobamide). These results indicate that canned escargots (boiled plain) are not good sources of vitamin B12 for humans.

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Teng, F. , Tanioka, Y. , Bito, T. , Takenaka, S. , Yabuta, Y. and Watanabe, F. (2015) Occurrence of Biologically Inactive Corrinoid Compounds in Canned Edible Apple Snails (Escargots). Food and Nutrition Sciences, 6, 1071-1077. doi: 10.4236/fns.2015.612111.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Abdulmawjood, A. and Bülte, M. (2001) Snail Species Identification by RFLP-PCR and Designing of Species-Specific Oligonucleotide Primers. Journal of Food Science, 66, 1287-1293.
[2] Watanabe, F. (2007) Vitamin B12 Sources and Bioavailability. Experimental Biology and Medicine, 232, 1266-1274.
[3] Herbert, V. (1996) Vitamin B12. Present Knowledge in Nutrition. 7th Edition, International Life Sciences Institute Press, Washington DC, 191-205.
[4] Watanabe, F., Katsura, H., Takenaka, S., Fujita, T., Abe, K., Tamura, Y., Nakatsuka, T. and Nakano, Y. (1999) Pseudovitamin B12 Is the Predominant Cobamide of an Algal Health Food, Spirulina Tablets. Journal of Agricultural and Food Chemistry, 47, 4736-4741.
[5] Tanioka, Y., Takenaka, S., Furusho, T., Yabuta, Y., Nakano, Y. and Watanabe, F. (2014) Identification of Vitamin B12 and Pseudovitamin B12 from Various Edible Shellfish Using Liquid Chromatography—Electrospray Ionization/Tan-dem Mass Spectrometry. Fisheries Science, 80, 1065-1071.
[6] Tanioka, Y., Yabuta, Y., Miyamoto, E., Inui, H. and Watanabe, F. (2008) Analysis of Vitamin B12 in Food by Silica Gel 60 TLC and Bioautography with Vitamin B12-Dependent Escherichia coli 215. Journal of Liquid Chromatography & Related Technologies, 3, 1977-1985.
[7] Ministry of Education, Culture, Sports, Science and Technology (2010) Report of the Subdivision of Resources. Standard Tables of Food Composition in Japan-2010. The Council for Science and Technology, Ministry of Education, Culture, Sports, Science and Technology, Tokyo, 170-173.
[8] Ueta, K., Takenaka, S., Yabuta, Y. and Watanabe, F. (2011) Broth from Canned Clams Is Suitable for Use as an Excellent Source of Free Vitamin B12. Journal of Agricultural and Food Chemistry, 59, 12054-12058.

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