In Vitro Evaluation of Sida pilosa Retz (Malvaceae) Aqueous Extract and Derived Fractions on Schistosoma mansoni
Hermine Boukeng Jatsa1,2,3*, Cintia Aparecida de Jesus Pereira2, Ana Bárbara Dias Pereira4, Deborah Aparecida Negrão-Corrêa2, Fernão Castro Braga4, Glauber Meireles Maciel5, Rachel Oliviera Castilho5, Pierre Kamtchouing1, Mauro Martins Teixeira3
1Laboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.
2Laboratory of Schistosomiasis, Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
3Laboratory of Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
4Laboratory of Phytochemistry, Department of Pharmaceutical Products, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil.
5Laboratory of Pharmacognosy, Department of Pharmaceutical Products, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil.
DOI: 10.4236/pp.2015.68039   PDF   HTML   XML   3,506 Downloads   4,717 Views   Citations


Sida pilosa Retz. (Malvaceae) is a medicinal plant used in Africa for the treatment of dysmenorrhea, lower abdominal pains and intestinal helminthiasis. S. pilosa aqueous extract and derived fractions were investigated for their bioactivity against Schistosoma mansoni. The aqueous extract from S. pilosa aerial parts (1.25 - 40 mg/mL) and derived fractions (n-hexane, DCM, EtOAc and n-BuOH: 0.25 - 8 mg/mL) were tested on adult S. mansoni maintained in a GMEN culture medium. Praziquantel was used as the reference drug. After 24 h of incubation, worms were monitored for their viability and egg output. The antioxidant activity of S. pilosa was evaluated by the ability to scavenge the 2,2-diphenyl-1-picrylhydrazyl free radicals. The chemical composition of the n-BuOH fraction was investigated by HPLC-MS analysis. S. pilosa aqueous extract and fractions significantly increased worm mortality in a concentration-dependent manner. The n-BuOH fraction was the most active with a LC50 of 1.25 mg/mL. Significant reduction of motor activity (25% to 100%) was recorded for surviving worms incubated in different concentrations of the extract and fractions. Incubation of S. mansoni in different concentrations of S. pilosa extract and fractions led to significant reduction of egg laying (52% to 100%). The aqueous extract and derived fractions exhibited antioxidant activity in a concentration-dependent manner. The highest antioxidant activity was found with the EtOAc fraction, followed by the DCM and n-BuOH fractions. HPLC-MS analysis of the n-butanol fraction revealed the presence of two indoloquinoline alkaloids. This study disclosed the schistosomicidal activity of the n-butanol fraction from S. pilosa aqueous extract. This activity is probably related to the indoloquinoline alkaloids identified in the fraction.

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Boukeng Jatsa, H. , de Jesus Pereira, C. , Dias Pereira, A. , Negrão-Corrêa, D. , Castro Braga, F. , Meireles Maciel, G. , Oliviera Castilho, R. , Kamtchouing, P. and Martins Teixeira, M. (2015) In Vitro Evaluation of Sida pilosa Retz (Malvaceae) Aqueous Extract and Derived Fractions on Schistosoma mansoni. Pharmacology & Pharmacy, 6, 380-390. doi: 10.4236/pp.2015.68039.

Conflicts of Interest

The authors declare no conflicts of interest.


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