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Seed Germination and in Vitro Organogenesis Optimization of Zhumeria majdae (Lamiaceae) from Iran

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DOI: 10.4236/ajps.2015.611185    2,443 Downloads   2,987 Views   Citations

ABSTRACT

Zhumeria majdae is a medicinal plant and endemic of Iran that is being threatened to extinction. The present investigation to preserve this relict species, was conducted to study of: 1) different treatments effect on seeds germination: a) in vivo, b) in vitro were subjected to 5 treatments, 2) callus induction and organogenesis from leaf, root and stem of explants by tissue culture on MS medium supplemented with 7 different concentration and combinations of auxin and cytokinin. Results indicated the germination rate were promoted by seeds mucilage, and were inhibited under cold condition. The best callus induction were observed from stem and leaf explants in the MS + B(IAA(0.1) + Kin(1) mg·L-10). The maximum leaf formation of leaf explants were produced in the MS + E(IAA(0.1) + BA(2) mg·L-10).

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Aghazadeh, P. and Behboodi, B. (2015) Seed Germination and in Vitro Organogenesis Optimization of Zhumeria majdae (Lamiaceae) from Iran. American Journal of Plant Sciences, 6, 1850-1856. doi: 10.4236/ajps.2015.611185.

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