Purification of the Drosophila melanogaster Proteins Inscuteable and Staufen Expressed in Escherichia coli

Xristo Zárate^1*, Megan M. McEvoy², Teresa Vargas-Cortez¹, Jéssica J. Gómez-Lugo¹, Claudia J. Barahona¹, Elena Cantú- Cárdenas¹, Alberto Gómez -Treviño¹

¹Universidad Autónoma de Nuevo León, Facultad de Ciencias Químicas, Cd. Universitaria, San Nicolás de los Garza, México.
²Department of Chemistry and Biochemistry, University of Arizona, Tucson, USA.

DOI: 10.4236/abb.2015.67050                

Abstract

The proteins Inscuteable and Staufen are key components during asymmetric cell division of neuroblasts for the development of Drosophila melanogaster. Expression and purification of both proteins has been a difficult task for structure-function studies. Based on codon optimization for protein expression in Escherichia coli, we have been able to produce, in soluble form, the C-terminal domains of Inscuteable and Staufen as chimeras with N-terminal maltose binding protein tag that contains a rigid linker between them for feasible crystallization. In addition, using an optimized synthetic gene, corresponding to the amino acid region 250 - 623 of Inscuteable fused to glutathione-S-transferase, low-scale expression experiments showed production of soluble protein. Finally, eukaryotic expression of Inscuteable in the methylothropic yeast Pichia pastoris failed to produce the Drosophila protein at detectable amounts, reinforcing the fact that E. coli still was the microorganism of choice for high-yield protein expression.

Keywords

Inscuteable, Staufen, Protein Expression and Purification, Maltose-Binding Protein, Escherichia coli

Conflicts of Interest

The authors declare no conflicts of interest.

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