Effects of Streptomyces Biofertilizer to Soil Fertility and Rhizosphere’s Functional Biodiversity of Agricultural Plants

Abstract Full-Text HTML XML Download Download as PDF (Size:1167KB) PP. 555-571
DOI: 10.4236/aim.2015.57058    2,803 Downloads   3,837 Views   Citations


In the present study, a biofertilizer on the basis of Streptomyces fumanus gn-2 was used for the treatment of wheat and soybean seeds (dose 104 spore/ml) before planting them in soil with low fertility in order to determine the effect of this biological agent on germination rate; the growth of seedlings, shoots, and the maturation phase of plants; the rhizosphere’s functional biodiversity; and the resistance of these plants to pathogens. Seeds were soaked in the suspension for a period of two or three hours. During the growing season of the crop, no additional fertilizing and spraying of a biopesticide against diseases or pests occurred. Despite the soil having low fertility, low quantities of organic matter, and not having been before used for the cultivation of agricultural plants, this biofertilizer showed a strong stimulatory effect on the growth of seeds and seedlings of wheat and soybeans. The average germination and seed vigor increased by 1.5 - 2.0 times, and the phenophases were accelerated to three to five days. In all phases of vegetation, the ammonifying bacteria in the presence of an antagonist (a biological agent) developed rapidly and were constantly present in significant numbers in the rhizosphere. Streptomyces fumanus introduced into non-sterile soil entered into competition with the local soil microflora and had the ability to colonize the rhizosphere system of plants. The use of a formulation of Streptomyces gn-2 has improved the composition of rhizosphere microflora, attracting saprophytic microorganisms: ammonificators and oligotrophs. The presence of the biocontrol microorganism Streptomyces fumanus in the rhizosphere plays an important role in enhancing the growth and development of useful groups, such as nitrogen-fixing bacteria.

Cite this paper

Doolotkeldieva, T. , Bobusheva, S. and Konurbaeva, M. (2015) Effects of Streptomyces Biofertilizer to Soil Fertility and Rhizosphere’s Functional Biodiversity of Agricultural Plants. Advances in Microbiology, 5, 555-571. doi: 10.4236/aim.2015.57058.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] ISTA (1996) International Rules for Seed Testing. Seed Science and Technology, 13, 299-513.
[2] Mogle, U.P. and Mane, R.Y. (2010) Antagonistic Effect of Biofertilizers against Seed Borne My Coflora of Tomato (Lycopersicum esculentum). Research Journal of Agricultural Sciences, 1, 255-258.
[3] Bharathi, V., Sudhakar, R., Parimala, K. and Reddy, V.A. (2013) Evaluation of Bioagents and Biofertilizers for the Management of Seed and Seedling Diseases of Sesamum indicum (Sesame). eSci Journal of Plant Pathology, 2, 179-186.
[4] Corte, L., Dell’Abate, M.T., Magini, A., Migliore, M., Felici, B., Roscini, L., et al. (2014) Assessment of Safety and Efficiency of Nitrogen Organic Fertilizers from Animal-Based Protein Hydrolysates—A Laboratory Multidisciplinary Approach. Journal of the Science of Food and Agriculture, 94, 235-245. http://dx.doi.org/10.1002/jsfa.6239
[5] Frankenberger, W.T. and Arshad, M. (1995) Phytormones in Soils. Marcel Dekker Inc., New York, 35-71.
[6] Jindo, K., Martim, S.A., Navarro, E.C., Pérez-Alfocea, F., Hernandez, T., Garcia, C., et al. (2012) Root Growth Promoting by Humic Acids from Composted and Non-Composted Urban Organic Wastes. Plant and Soil, 353, 209-220.
[7] Pizzeghello, D., Francioso, O., Ertani, A., Muscolo, A. and Nardi, S. (2013) Isopentenyladenosine and Cytokinin-Like Activity of Different Humic Substances. Journal of Geochemical Exploration, 129, 70-75.
[8] Canellas, L.P., Olivares, F.L., Okorokova-Facanha, A.L. and Facanha, A.R. (2002) Humic Acids Isolated from Earthworm Compost Enhance Root Elongation, Lateral Rootemergence, and Plasma Membrane H+-ATPase Activity in Maize Roots. Plant Physiology, 130, 1951-1957. http://dx.doi.org/10.1104/pp.007088
[9] Nardi, S., Pizzeghello, D., Muscolo, A. and Vianello, A. (2002) Physiological Effects of Humic Substances on Higher Plants. Soil Biology & Biochemistry, 34, 1527-1536.
[10] Khan, W., Rayirath, U.P., Subramanian, S., Jithesh, M.N., Hodges, D.M., Critchley, A.T., et al. (2009) Seaweed Extracts as Biostimulants of Plant Growth and Development. Plant Growth Regulation, 28, 386-399.
[11] Nardi, S., Muscolo, A., Vaccaro, S., Baiano, S., Spaccini, R. and Piccolo, A. (2007) Relationships between Molecular Characteristics of Soil Humic Fractions and Glycolytic Pathway and Krebs Cycle in Maize Seedlings. Soil Biology and Biochemistry, 39, 3138-3146.
[12] Nardi, S., Carletti, P., Pizzeghello, D. and Muscolo, A. (2009) Biological Activities of Humic Substances. In: Senesi, N., Xing, B. and Huang, P.M., Eds., Biophysico-Chemical Processes Involving Natural Nonliving Organic Matter in Environmental Systems. Part I. Fundamentals and Impact of Mineral-Organic-Biota Interactions on the Formation, Transformation, Turnover, and Storage of Natural NonlivingOrganic Matter (NOM), John Wiley & Sons, Hoboken, 305-339. http://dx.doi.org/10.1002/9780470494950.ch8
[13] Schiavon, M., Ertani, E. and Nardi, S. (2008) Effects of an Alfalfa Protein Hydrolysate on the Gene Expression and Activity of Enzymes of TCA Cycle and N Metabolism in Zea mays L. Journal of Agricultural and Food Chemistry. 172, 237-244.
[14] Ertani, A., Nardi, S. and Altissimo, A. (2012) Review: Long-Term Research Activity on the Biostimulant Properties of Natural Origin Compounds. Acta Horticulturae, 1009, 181-188.
[15] Ertani, A., Schiavon, M., Altissimo, A., Franceschi, C. and Nardi, S. (2011) Phenol-Containing Organic Substances Stimulate Phenylpropanoid Metabolism in Zea mays. Journal of Plant Nutrition and Soil Science, 3, 496-503. http://dx.doi.org/10.1002/jpln.201000075
[16] Marfà, O., Cáceres, R., Polo, J. and Ródenas, J. (2009) Animal Protein Hydrolysate ASA Biostimulant for Transplanted Strawberry Plants Subjected to Cold Stress. Acta Horticulturae, 842, 315-318.
[17] Ertani, A., Schiavon, M., Muscolo, A. and Nardi, S. (2013) Alfalfa Plant-Derived Biostimulant Stimulate Short-Term Growth of Salt Stressed Zea mays L. Plants. Plant and Soil, 364, 145-148. http://dx.doi.org/10.1007/s11104-012-1335-z
[18] Lakhdar, A., Iannelli, M.A., Debez, A., Massacci, A., Jedidi, N. and Abdelly, C. (2010) Effect of Municipal solid Waste Compost and Sewage Sludge Use on Wheat (Triticum durum): Growth, Heavy Metal Accumulation, and Antioxidant Activity. Journal of the Science of Food and Agriculture, 90, 965-971. http://dx.doi.org/10.1002/jsfa.3904
[19] Nardi, S., Pizzeghello, D., Remiero, F. and Rascio, N. (2000) Chemical and Biochemical Properties of Humic Substances Isolated from Forest Soils and Plant Growth. Soil Science Society of America Journal, 64, 639-645.
[20] Zhang, X., Ervin, E.H. and Schmidt, R.E. (2003) Effects of Liquid Application of a Seaweed Extract and a Humic Acid on Creeping Bentgrass (Agrostis palustris Huds. A.). Journal of the American Society for Horticultural Science, 128, 492-496.
[21] Kaufmann III, G.L., Kneivel, D.P. and Watschke, T.L. (2007) Effects of a Biostimulant on the Heat Tolerance Associated with Photosynthetic Capacity, Membrane Thermostability, and Polyphenol Production of Perennial Ryegrass. Crop Science, 47, 261-267.
[22] Rose, M.T., Patti, A.F., Little, K.R., Brown, A.L., Jackson, W.R. and Cavagnaro, T.R. (2014) A Meta-Analysis and Review of Plant-Growth Response to Humic Substances: Practical Implications for Agriculture. Advances in Agronomy, 124, 37-89.
[23] Lehr, N.A., Schrey, S.D., Hampp, R. and Tarkka, M.T. (2008) Root Inoculation with a Forest Soil Streptomycete Leads to Locally and Systemically Increased Resistance against Phytopathogens in Norway Spruce. New Phytologist, 177, 965-976.
[24] Abd Allah, N.A. and El-Mehalawy, A.A. (2002) Antifungal Producing Actinomycetes as Biocontrol Agents for Plant Pathogenic Fungi. Al-Azhar Journal of Microbiology, 58, 51-60.
[25] Rothrock, C.S. and Gottlieb, D. (1984) Role of Antibiosis in Antagonism of Streptomyces hygroscopicus var. Geldans to Rhizoctonia solani in Soil. Canadian Journal of Microbiology, 30, 1440-1447. http://dx.doi.org/10.1139/m84-230
[26] Compant, S., Duffy, B., Nowak, J., Clement, C. and Barka, E.A. (2005) Use of Plant Growth-Promoting Bacteria for Biocontrol of Plant Diseases: Principles, Mechanisms of Action, and Future Prospects. Applied and Environmental Microbiology, 71, 4951-4959.
[27] Doolotkeldieva, T. (2010) Novel Antifungal and Bio Stimulator Microbial Products for Sustainable Crop Production in Kyrgyzstan. Materials of 3rd International Conference on the Organic Sector Development in Central Eastern European and Central Asian Countries, Astana, 17-20 September, 132-135.
[28] Doolotkeldieva, T. (2012) Chapter 14. Biological Control Agents for Crop Protection and Sustainability of Agro-Ecosystems in Kyrgyzstan. In: Pillarisetti, J.R., Lawrey, R. and Ahmad, A., Eds., Multifunctional Agriculture, Ecology and Food Security: International Perspectives, Nova Science Publishers, Hauppauge, 197-208.
[29] SAS Institute (1988) SAS/STAT User’s Guide. Release 6.03, 6th Editions, SAS Institute Inc., Cary, 1028.
[30] Nichiporovich, A.A. (1975) The Theory of Photosynthetic Productivity of Plants and Rational Directions of Breeding for Increasing the Productivity/Physiological and Genetic Basis of Increasing the Productivity of Crops: Collection of Scientific Papers. Kolos, Moscow, 5-14.
[31] Kruzhilin, A.S. (1975) Physiology of Development and Productivity of Plants: Collection of Scientific Papers. Kolos, Moscow, 53-63.
[32] Vasilenko, I.I. (1978) Features Photosynthetic Productivity and Yield for Mation of Winter Wheat Varieties of Intensive Type. Journal of Agricultural Science, 7, 18-26.
[33] Nannipieri, P., Ascher, J., Ceccherini, M.T., Landi, L., Pietramellara, G. and Renella, G. (2003) Microbial Diversity and Soil Functions. European Journal of Soil Science, 54, 655-670.
[34] Bastida, F., Zsolnay, A., Hernández, T. and García, C. (2008) Past, Present and Future of Soil Quality Indices: A Biological Perspective. Geoderma, 147, 159-171.
[35] Garland, J.L. and Mills, A.L. (1991) Classification and Characterization of Heterotrophic Microbial Communities on the Basis of Patterns of Community-Level Sole-Carbon-Source Utilization. Applied and Environmental Microbiology, 57, 2351-2359.
[36] Degens, B.P. and Harris, J.A. (1997) Development of a physiological approach to measuring the catabolic diversity of soil microbial communities. Soil Biology & Biochemistry, 29, 1309-1320.
[37] Campbell, C.D., Chapman, S.J., Cameron, C.M., Davidson, M.S. and Potts, J.M. (2003) A Rapid Microtiter Plate Method to Measure Carbon Dioxide Evolved from Carbon Substrate Amendments so as to Determine the Physiological Profiles of Soil Microbial Communities by Using Whole Soil. Applied and Environmental Microbiology, 69, 3593-3599.
[38] Degens, B.P., Schipper, L.A., Sparling, G.P. and Duncan, L.C. (2001) Is the Microbial Community in a Soil with Reduced Catabolic Diversity Less Resistant to Stress or Disturbance? Soil Biology and Biochemistry, 33, 1143-1153.

comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.