Polyphenol Oxidase Activity and Colour Changes of ‘Starking’ Apple Cubes Coated with Alginate and Dehydrated with Air

DOI: 10.4236/fns.2011.25064   PDF   HTML     8,593 Downloads   13,711 Views   Citations


The objective was to study the effect of alginate coating on polyphenol oxidase (PPO) activity and colour of ‘Starking’ apple cubes during dehydration with hot air. Apple cubes were dehydrated at 20oC, 35oC, or 40oC, with a parallel airflow. Analysis of PPO activity, colour (L*, a*, b*) and dry matter were performed along the dehydration process at each temperature. All samples presented a peak in relative PPO activity in the beginning of the drying. Exponential models fitted well the experimental data after the peak. Cubes without coating presented lower PPO activity, suggesting lower browning, than coated samples throughout the dehydration process, for all temperatures. Better results for coated samples were obtained with a perpendicular airflow drying at 40oC, after dipping the whole apple in water at 60oC for 10 min. In order to prevent coated samples from browning, drying by perpendicular airflow preceded by a thermal treatment of the whole apple is required.

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S. Fernandes, C. Ribeiro, M. Raposo, R. Morais and A. Morais, "Polyphenol Oxidase Activity and Colour Changes of ‘Starking’ Apple Cubes Coated with Alginate and Dehydrated with Air," Food and Nutrition Sciences, Vol. 2 No. 5, 2011, pp. 451-457. doi: 10.4236/fns.2011.25064.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] C. Y. Lee, “Enzymatic Browning Reaction,” In: F. J. Francis, Ed., Encyclopedia of Food Science and Technology (2nd Edition), Wiley, New York, 1999, pp. 494-515.
[2] J. Whiteker, “Principles of Enzymology for the Food Sciences,” M. Dekker, New York, 1972, pp. 571-577.
[3] G. I. Olivas and G. V. Barbosa-Cánovas, “Edible coatings for Fresh-Cut Fruits,” Critical Reviews of Food Science and Nutrition, Vol. 45, No. 7-8, 2005, pp. 657-670. doi:10.1080/10408690490911837
[4] S. L. S. Bico, M. F. J. Raposo, R. M. S. C. Morais and A. M. M. B. Morais, “Combined Effects of Chemical Dip and/or Carrageenan Coating and/or Controlled Atmosphere on Quality of Fresh-Cut Banana,” Food Control, Vol. 20, No. 5, 2009, pp. 508-514. doi:10.1016/j.foodcont.2008.07.017
[5] J. Y. Lee, H. J. Park, C. Y. Lee and W. Y. Choi, “Extending Shelf-Life of Minimally Processed Apples with Edible Coatings and Antibrowning Agents,” Lebensmittel-Wissenschaft and Technologie, Vol. 36, No. 3, 2003, pp. 323-329.
[6] S. L. S. Bico, M. F. J. Raposo, R. M. S. C. Morais and A. M. M. B. Morais, “Chemical Dips and Edible Coatings to Retard Softening and Browning of Fresh-Cut Banana,” International Journal of Postharvest Technology and Innovation, Vol. 2, No. 1, 2010, pp. 13-24.
[7] M. A. Rojas-Graü, M. S. Tapia and O. Martín-Belloso, “Using Polysaccharide-Based Edible Coatings to Maintain Quality of Fresh-Cut Fuji apples,” LWT-Food Science and Technology, Vol. 41, No. 1, 2008, pp. 139-147. doi:10.1016/j.lwt.2007.01.009
[8] M. M. Khin, W. Zhou and C. O. Perera, “Impact of Process Conditions and Coatings on the Dehydration Efficiency and Cellular Structure of Apple Tissue during Osmotic Dehydration,” Journal of Food Engineering, Vol. 79, No. 3, 2007, pp. 817-827. doi:10.1016/j.jfoodeng.2006.02.046
[9] G. I. Olivas, D. S. Mattinson and G. V. Barbosa- Cánovas, “Alginate Coatings for Preservation of Minimally Processed ‘Gala’ Apples,” Postharvest Biology and Technology, Vol. 45, No. 1, 2007, pp. 89-96. doi:10.1016/j.postharvbio.2006.11.018
[10] D. J. McHugh, “Alginate,” In: D. J. McHugh, Ed., A Guide to the Seaweed Industry, Fisheries and Aquaculture Department, 2003, pp. 39-50.
[11] M. Sen, “Effects of Molecular Weight and Ratio of Guluronic Acid to Mannuronic Acid on the Antioxidant Properties of Sodium Alginate Fractions Prepared by Radiation-Induced Degradation,” Applied Radiation and Isotopes, Vol. 69, No. 1, 2011, pp.126-129. doi:10.1016/j.apradiso.2010.08.017
[12] H. Trommer and R. H. H. Neubert, “The Examination of Polysaccharides as Potential Antioxidative Compounds for Topical Administration Using a Lipid Model System,” International Journal of Pharmaceutics, Vol. 298, No. 1, 2005, pp. 153-163. doi:10.1016/j.ijpharm.2005.04.024.
[13] M. A. Rojas-Graü, M. S. Tapia, F. J. Rodriguez, A. J. Carmona and O. Martín-Belloso, “Alginate and Gellan-based Edible Coatings as Carriers of Antibrowning Agents Applied on Fresh-Cut ‘Fuji’ Apples,” Food Hydrocolloids, Vol. 21, No. 1, 2007, pp. 118-127. doi:10.1016/j.foodhyd.2006.03.001
[14] J. D. Ponting, R. Jackson and G. Walters, “Refrigerated Apple Slices: Effects of pH, Sulfites and Calcium on Texture,” Journal of Food Science, Vol. 36, No. 2, 1971, pp. 449-450. doi:10.1111/j.1365-2621.1971.tb04059.x
[15] A. Lenart and D. Piotrowski, “Drying Characteristics of Osmotically Dehydrated Fruits Coated with Semipermeable Edible Films,” Drying Technology, Vol. 19, No. 5, 2001, pp. 849-877. doi:10.1081/DRT-100103772
[16] M. M. Khin, W. Zhou and C. O.Perera, “A Study of the Mass Transfer in Osmotic Dehydration of Coated Potato Cubes,” Journal of Food Engineering, Vol. 77, No. 1, 2006, pp.84-95. doi:10.1016/j.jfoodeng.2005.06.050
[17] Commission Internationale de L’Eclairage (CIE), “Recommendations on Uniform Colour Spaces, Colour-Difference Equations, Psychometric Colour Terms,” CIE, Paris, 1978.
[18] A. M. C. N. Rocha and A. M. M. B. Morais, “Polyphenoloxidase Activity and Total Phenolic Content as Related to Browning of Minimally Processed ‘Jonagored’ Apple,” Journal of the Science of Food and Agriculture, Vol. 82, No. 1, 2001, pp. 120-126. doi:10.1002/jsfa.1006
[19] E. Cantos, J. A. Tudela, M. I. Gil and J. C. Espín, “Phenolic Compounds and Related Enzymes are not Rate- Limiting in Browning Development of Fresh-Cut Potatoes,” Journal of Agriculture and Food Chemistry, Vol. 50, No. 10, 2002, pp. 3015-3023. doi:10.1021/jf0116350
[20] A. M. C. N. Rocha, C. Mota and A. M. M. B. Morais, “Efeito de Armazenamento sob Vácuo na Actividade da PPO e nos Compostos Fenólicos da Cenoura Descascada (cv. Nantes),” Proceedings of 7o Encontro de Química de Alimentos, Viseu, 13-16 April 2005, in CD- ROM.
[21] M. Karel, “Water Activity and Food Preservation,” In: M. Karel, O. R. Fennema and D. B. Lund, Eds., Principles of Food Science (Part II), Marcel Dekker, New York, 1975, pp. 237-263.
[22] M. P. Cano, M. G. Lobo, B. de Ancos and M. A. M. Galeazzi, “Polyphenoloxidase from Spanish Hermaphrodite and Female Papaya Fruits (Carica papaya cv. Sunrise, Solo Group),” Journal of Agriculture and Food Chemistry, Vol. 44, No. 10, 1996, pp. 3075-3079. doi:10.1021/jf960119k
[23] D. M. N. Eidhin, E. Murphy and D. O’Beirne, “Polyphenol oxidase from Apple (Malusdomestica borkh. cv. Bramley’s Seedling): Purification Strategies and Characterization,” Journal of Food Science, Vol. 71, No. 1, 2006, pp. C51-C58. doi:10.1111/j.1365-2621.2006.tb12388.x
[24] Y. Jiang, “Properties of Litchi Polyphenoloxidase,” Acta Horticulturae (ISHS), Vol. 558, 2001, pp. 367- 373.
[25] G. P. Mizobutsi, F. L. Finger, R. A. Ribeiro, R. Puschmann, L. L. M. Neves, and W. F. da Mota, “Effect of pH and Temperature on Peroxidase and Polyphenol- Oxidase Activities of Litchi Pericarp,” Science and Agriculture, Vol. 67, No. 2, 2010, pp.213-217.
[26] Z. Emam-Djomeh, J. Dehghannya and R. Sotudeh-Gharabagh, “Assessment of Osmotic Process in Combination with Coating on Effective Diffusivities during Drying of Apple Slices,” Drying Technology, Vol. 24, No. 9, 2006, pp. 1159-1164. doi:10.1080/07373930600778437
[27] I. Damaldi, G. Rapeanu, A. van Loey, C. Smout and M. Hendrickx, “Characterization and Inactivation by Thermal and Pressure Processing of Strawberry (Fragaria ananassa) Polyphenol Oxidase: A Kinetic Study,” Journal of Food Biochemistry, Vol. 30, No. 1, 2006, pp. 56-76. doi:10.1111/j.1745-4514.2005.00045.x
[28] D. M. Kim, N. L. Smith and C. Y. Lee, “Effect of Heat Treatment on Firmness of Apples and Apple slices,” Journal of Food Processing and Preservation, Vol. 18, No. 1, 1994, pp. 1-8. doi:10.1111/j.1745-4549.1994.tb00237.x

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