Antifungal Activity against Aspergillus parasiticus of Supernatants from Whey Permeates Fermented with Kefir Grains
Raúl Ricardo Gamba1,2,3, Carolina Ni Colo1, Mariana Correa1,3, Andrea Astoreca3,4, Teresa Alconada3,4, Graciela De Antoni1,2,5, Angela León Peláez1
1Cátedra de Microbiología, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentine.
2CIDCA (Centro de Investigación y Desarrollo en Criotecnología de los Alimentos), La Plata, Argentine.
3CONICET (Consejo Nacional de Investigaciones Científicas y Tecnológicas), CCT-La Plata, La Plata, Argentine.
4CINDEFI (Centro de Investigación y Desarrollo en Fermentaciones Industriales), Universidad Nacional de La Plata, La Plata, Argentine.
5CIC-PBA (Comisión de Investigaciones Científicas-Provincia de Buenas Aires), Buenas Aires, Argentine.
DOI: 10.4236/aim.2015.56049   PDF   HTML   XML   3,592 Downloads   4,660 Views   Citations


Aspergillus parasiticus, a common fungal contaminant in food, produces aflatoxin B1, which is classified as human carcinogen. Kefir is an ancient fermented beverage obtained by the fermentation of different substrates with kefir grains. A very important waste produced by the dairy cheese industry is the whey permeate, which nowadays is a strong ambient contaminant. The aim of this work was to assess the effect of whey permeates fermented with kefir grains against A. parasiticus growth, aflatoxin B1 biosynthesis, and the kefir microorganisms protection against the cell damage produced by aflatoxin B1. It was observed that kefir-cell-free-supernatants (CFS) produced fungal inhibition. A fungicidal effect was observed with 65% v/v of CFS in the culture medium (final pH 4.55 and total undissociated lactic and acetic acid concentration 34.08 mM). Under these conditions, aflatoxin production was not detected. Finally, it was found that non-viable kefir microorganisms protected HepG2 cells from the damage produced by aflatoxin B1.

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Gamba, R. , Colo, C. , Correa, M. , Astoreca, A. , Alconada, T. , Antoni, G. and Peláez, A. (2015) Antifungal Activity against Aspergillus parasiticus of Supernatants from Whey Permeates Fermented with Kefir Grains. Advances in Microbiology, 5, 479-492. doi: 10.4236/aim.2015.56049.

Conflicts of Interest

The authors declare no conflicts of interest.


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