Monolayers Langmuir-Blodgett Films of Synthetic Artificial Mimic Molecules That Resemble the Following Tetraether Lipids on Silicon Wafers

Abstract

This study investigated the behavior and molecular organization of synthetic artificial mimic molecules that resemble the following tetraether lipids: di-O-hexadecyl-glycero-3-phosphatidyl-glycerol (DHGPG) and bis-4-dodecylphenyl-12-phosphate. These molecules were analyzed using Langmuir film balance, ellipsometry and atomic force microscopy. The monolayer Langmuir-Blodgett films of DHGPG and bis-4-dodecylphenyl-12-phosphate were stable on the solid surface silicon wafers. The ellipsometry and AFM results showed that monolayers Langmuir-Blodgett films of DHGPG and bis-4-dodecylphenyl-12-phosphate were present, and the thickness of the observed films varied from 1.2 - 5.0 nm.

Share and Cite:

Vidawati, S. and Rothe, U. (2015) Monolayers Langmuir-Blodgett Films of Synthetic Artificial Mimic Molecules That Resemble the Following Tetraether Lipids on Silicon Wafers. Advances in Biological Chemistry, 5, 189-196. doi: 10.4236/abc.2015.54015.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Weissbuch, I., Baxter, P.N.W., Cohen, S., Cohen, H., Kjaer, K., Howes, P.B., Als-Nielsen, J., Hanan, G.S., Schubert, U.S., Lehn, J.-M., Leiserowitz, L. and Lahav, M.J. (1998) Self-Assembly at the Air-Water Interface. In-Situ Preparation of Thin Films of Metal Ion Grid Architectures. Journal of the American Chemistry Society, 120, 4850-4860.
http://dx.doi.org/10.1021/ja980205w
[2] Swift, J.A., Pivovar, A.M., Reynolds, A.M. and Ward, M.D. (1998) Template-Directed Architectural Isomerism of Open Molecular Frameworks:? Engineering of Crystalline Clathrates. Journal of the American Chemistry Society, 120, 5887-5894.
http://dx.doi.org/10.1021/ja980793d
[3] Sengupta, K., Schilling, J., Marx, S., Fischer, M., Bacher, A. and Sackmann, E. (2003) Mimicking Tissue Surfaces by Supported Membrane Coupled Ultrathin Layer of Hyaluronic Acid. Langmuir, 19, 1775-1781.
http://dx.doi.org/10.1021/la026146o
[4] Stern, J., Freisleben, H.J., Janku, S. and Ring, K. (1992) Black Lipid Membranes of Tetraether Lipids from Thermoplasma acidophilum. Biochimica et Biophysica Acta, 1128, 227-236.
http://dx.doi.org/10.1016/0005-2760(92)90312-J
[5] Fuhrhop, J., David, H.H., Mathieu, J., Liman, U., Winter, H.J. and Boekema, E.J. (1986) Bolaamphiphiles and Monolayer Lipid Membranes Made from 1,6,19,24-Tetraoxa-3,21-cyclohexatriacontadiene-2,5,20,23-tetrone. Journal of the American Chemistry Society, 108, 1785-1791.
http://dx.doi.org/10.1021/ja00268a013
[6] Bode, M.L., Buddoo, S.R., Minnaar, S.H. and Du Plessis, C.A. (2008) Extraction, Isolation and NMR Data of the Tetraether Lipid Calditoglycerocaldarchaeol (GDNT) from Sulfolobus metallicus Harvested from a Bioleaching Reactor. Chemistry and Physics of Lipids, 154, 94-104.
http://dx.doi.org/10.1016/j.chemphyslip.2008.02.005
[7] Vidawati, S., Rothe, U. and Bakowsky, U. (2012) Langmuir Films Stability Phenomenon of Glycerol Dialkyl Nonitol Tetraether at the Air-Water Interface for Variations Spreading Time. Advances in Biological Chemistry, 2, 233-237.
http://dx.doi.org/10.4236/abc.2012.23028
[8] Vidawati, S., Sitterberg, J., Bakowsky, U. and Rothe, U. (2010) AFM and Ellipsometric Studies on LB Films of Natural Asymmetric and Symmetric Bolaamphiphilic Archaebacterial Tetraether Lipids on Silicon Wafers. Colloids and Surfaces B: Biointerfaces, 78, 303-309.
http://dx.doi.org/10.1016/j.colsurfb.2010.03.015
[9] Vidawati, S., Sitterberg, J., Rothe, U. and Bakowsky, U. (2011) Stability of Monomolecular Films of Archaebacterial Tetraether Lipids on Silicon Wafers: A Comparison of Physisorbed and Chemisorbed Monolayers. Colloids and Surfaces B: Biointerfaces, 87, 209-216.
http://dx.doi.org/10.1016/j.colsurfb.2011.05.005
[10] Bakowsky, U., Rothe, U., Antonopoulos, E., Martini, T., Henkel, L. and Freisleben, H.J. (2000) Monomolecular Organization of the Main Tetraether Lipid from Thermoplasma acidophilum at the Water-Air Interface. Chemistry and Physics of Lipids, 105, 31-42.
http://dx.doi.org/10.1016/S0009-3084(99)00131-0
[11] Gabriel, J.L., Chong, P.L.G. (2000) Molecular Modeling of Archaebacterial Bipolar Tetraether Lipid Membranes. Chemistry and Physics of Lipids, 105, 193-200.
http://dx.doi.org/10.1016/S0009-3084(00)00126-2
[12] Rolandi, R., Schindler, H., De Rosa, M. and Gambacorta, A. (1986) Monolayers of Ether Lipids from Archaebacteria, European Biophysics Journal, 14, 19-27.
http://dx.doi.org/10.1007/BF00260399
[13] Dote, J.L., Barger, W.R., Behroozi, F., Chang, E.L., Lo, S.L., Montague, C.E. and Nagumo, M. (1990) Monomolecular Film Behavior of Tetraether Lipids from a Thermoacidophilic Archaebacterium at the Air/Water Interface. Langmuir, 6, 1017-1023.
http://dx.doi.org/10.1021/la00095a023
[14] Elferinck, M.G.L., De Wit, J.G., Demel, R., Driessen, A.J.M. and Konings, W.N.J. (1992) Functional Reconstitution of Membrane Proteins in Monolayer Liposomes from Bipolar Lipids of Sulfolobus acidocaldarius. Journal of Biological Chemistry, 267, 1375-1381.
[15] De Rosa, M. (1996) Archaeal Lipids: Structural Features and Supramolecular Organization. Thin Solid Films, 284-285, 13-17.
http://dx.doi.org/10.1016/S0040-6090(96)08832-3
[16] Dante, S., De Rosa, M., Maccioni, B., Morana, A., Nicolini, C., Rustichelli, F., Troitsky, V.I. and Yang, B. (1995) Thermal, stability of bipolar lipid Langmuir Blodgett films by X-Ray diffraction. Molecular Crystals and Liquid Crystals Science and Technology, 262, 191-207.
http://dx.doi.org/10.1080/10587259508033525

Copyright © 2022 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.