Genetic Association of Interferon Gamma Induced Protein-10 (IP-10), CXCL-10 Gene Polymorphisms with TB Pleurisy Susceptibility in South Indian Population


CXCL-10 known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine 10 is a 8.7 kDa protein, which is secreted in response to IFN-γ by monocytes, endothelial cells and fi-broblasts. It has chemo-attraction for monocytes/macrophages, T cells, NK cells and dendritic cells in promotion of T cell adhesion to endothelial cells. In the present study, we investigated whether polymorphisms in CXCL-10 gene have any role in the manifestation of Tuberculous (TB) pleurisy. Two SNPs in CXCL-10 promoter region (1447A > G and 135G > A) were genotyped in patients with TB Pleurisy (n = 186), Pulmonary TB patients (n = 159) and healthy controls (n = 205) by PCR-RFLP. Disease associations were statistically analyzed by Fisher exact test. At the 135G > A position, the frequencies of genotype GA and allele G were significantly high in TB pleurisy patients compared to healthy controls. While the frequencies of genotype AA and allele A were significantly low in TB pleurisy patients compared to healthy controls. The frequency of haplotype A-G with the combination of 1447A > G and 135G > A was significantly high in TB pleurisy. Our results reveal that genotype GA and allele G at 135G > A position were strongly associated with susceptibility to tuberculous pleurisy. The GA genotype may be a useful genetic marker for early detection of the disease in high risk individuals.

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Sheikh, G. , Neela, V. , Pydi, S. , Suryadevara, N. , Gaddam, R. , Gaddam, S. , Auzumeedi, S. and Valluri, V. (2015) Genetic Association of Interferon Gamma Induced Protein-10 (IP-10), CXCL-10 Gene Polymorphisms with TB Pleurisy Susceptibility in South Indian Population. Open Journal of Immunology, 5, 72-78. doi: 10.4236/oji.2015.52008.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] World Health Organization (2012) WHO: Global Tuberculosis Report. WHO, Geneva, 100.
[2] Light, R.W. (2010) Update on Tuberculous Pleural Effusion. Respirology, 15, 451-458.
[3] Sharma, S.K. and Mohan, A. (2004) Extrapulmonary Tuberculosis. Indian Journal of Medical Research, 120, 316-353.
[4] Udwadia, Z.F. and Sen, T. (2010) Pleural Tuberculosis: An Update. Current Opinion in Pulmonary Medicine, 16, 399- 406.
[5] Yoshie, O., Imai, T. and Nomiyama, H. (2001) Chemokines in Immunity. Advances in Immunology, 78, 57-110.
[6] Murdoch, C. and Finn, A. (2000) Chemokine Receptors and Their Role in Inflammation and Infectious Diseases. Blood, 95, 3032.
[7] Zlotnik, A. and Yoshie, O. (2000) Chemokines: A New Classification System and Their Role in Immunity. Immunity, 12, 121.
[8] O’Donovan, N., Galvin, M. and Morgan, J.G. (1999) Physical Mapping of the CXC Chemokine Locus on Human Chromosome 4. Cytogenetic and Genome Research, 84, 39-42.
[9] Dufour, J.H., Dziejman, M., Liu, M.T., Leung, J.H., Lane, T.E. and Luster, A.D. (2002) IFN-Gamma-Inducible Protein 10 (IP-10; CXCL10)-Deficient Mice Reveal a Role for IP-10 in Effector T Cell Generation and Trafficking. Journal of Immunology, 168, 3195-3204.
[10] Angiolillo, A.L., Sgadari, C., Taub, D.D., Liao, F., Farber, J.M., Maheshwari, S., Kleinman, H.K., Reaman, G.H. and Tosato, G. (1995) Human Interferon-Inducible Protein 10 Is a Potent Inhibitor of Angiogenesis in Vivo. Journal of Experimental Medicine, 182, 155-162.
[11] Hill, A.V. (2001) The Genomics and Genetics of Human Infectious Disease Susceptibility. Annual Review of Genomics and Human Genetics, 2, 373-400.
[12] Hill, A.V. (2006) Aspects of Genetic Susceptibility to Human Infectious Diseases. Annual Review of Genetics, 40, 469-486.
[13] Bellamy, R. (2006) Genome-Wide Approaches to Identifying Genetic Factors in Host Susceptibility to Tuberculosis. Microbes Infect, 8, 1119-1123.
[14] Navratilova, Z. (2006) Polymorphisms in CCL2 & CCL5 Chemokines/Chemokine Receptors Genes and Their Association with Diseases. Biomedical Papers, 150, 191-204.
[15] Smith, M.W., Dean, M., Carrington, M., et al. (1997) Contrasting Genetic Influence of CCR2 and CCR5 Variants on HIV-1 Infection and Disease Progression: Hemophilia Growth and Development Study (HGDS), Multicenter AIDS Cohort Study (MACS), Multicenter Hemophilia Cohort Study (MHCS), San Francisco City Cohort (SFCC), ALIVE Study. Science, 277, 959-965.
[16] Deng, G., Zhou, G., Zhang, R., et al. (2008) Regulatory Polymorphisms in the Promoter of CXCL10 Gene and Disease Progression in Male Hepatitis B Virus Carriers. Gastroenterology, 134, 716-726.
[17] Tang, N.L., Chan, P.K., Wong, C.K., et al. (2005) Early Enhanced Expression of Interferon-Inducible Protein-10 [CXCL-10] and Other Chemokines Predict Adverse Outcome in Severe Acute Respiratory Syndrome. Clinical Chemistry, 51, 2333-2340.
[18] Tang, N.L., Fan, H.P., Chang, K.C., et al. (2009) Genetic Association between a Chemokines Gene CXCL-10 (IP-10, Interferon Gamma Inducible Protein 10) and Susceptibility to Tuberculosis. Clinica Chimica Acta, 406, 98-102.
[19] Gupta, B.K., Bharat, V. and Bandyopadhyay, D. (2010) Sensitivity, Specificity, Negative and Positive Predictive Values of Adenosine Deaminase in Patients of Tubercular and Non-Tubercular Serosal Effusion in India. Journal of Clinical Medicine Research, 2, 121-126.
[20] Moller, M. and Hoal, E.G. (2010) Current Findings, Challenges and Novel Approaches in Human Genetic Susceptibility to Tuberculosis. Tuberculosis (Edinb), 90, 71-83.

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