Lily bulb Nectar Produces Expectorant and Anti-Tussive Activities, and Suppresses Cigarette Smoke-Induced Inflammatory Response in the Respiratory Tract in Mice


Air pollutants pose a major environmental threat to the respiratory system. Pathogen invasion and the exposure to particulate matters in atmospheric air, particularly, cigarette smoke (CS), have been found to be associated with acute and chronic respiratory diseases, including asthma. Therefore, the search for agents that can protect the respiratory system against potentially harmful substances is of interest in preventive health. Lily bulb Nectar (LBN), which contains Lily bulb, Pyrus pyrifolia N., Siraitia grosvenorii and Apricot kernel as its ingredients, is a health supplement intended to improve the wellness of the respiratory system in humans. Lily bulb, Pyrus pyrifolia N., Siraitia grosvenorii and Apricot kernel are commonly prescribed for the treatment of respiratory tract disorders such as bronchitis, pneumonia and cough in the practice of traditional Chinese medicine. Pharmacological studies have shown that these herbs can produce beneficial effects on the respiratory tract or even the lungs. In the present study, we investigated the effects of LBN on mouse respiratory tract function under normal and challenged conditions. LBN was first examined for its expectorant and anti-tussive activities in mice. The effect of LBN on long-term exposure to CS was also investigated. Our findings showed that long-term LBN treatment enhanced the expectorant activity and suppressed the SO2-induced coughing in mice. LBN treatment also suppressed the CS-induced inflammation in the respiratory tract, as assessed by differential cell count and cytokine production. In conclusion, long-term LBN consumption may produce beneficial effects on the respiratory tract function in humans, particularly in the face of challenge by irritants in the inhaling air.

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Wong, H. , Zou, S. , Li, J. , Ma, C. , Chen, J. , Leong, P. , Leung, H. , Chan, W. and Ko, K. (2015) Lily bulb Nectar Produces Expectorant and Anti-Tussive Activities, and Suppresses Cigarette Smoke-Induced Inflammatory Response in the Respiratory Tract in Mice. Chinese Medicine, 6, 136-146. doi: 10.4236/cm.2015.62015.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Mayer, H. (1999) Air Pollution in Cities. Atmospheric Environment, 33, 4029-4037.
[2] Kampa, M. and Castanas, E. (2008) Human Health Effects of Air Pollution. Environmental Pollution, 151, 362-367.
[3] Seaton, A., Godden, D., MacNee, W. and Donaldson, D. (1995) Particulates Air Pollution and Acute Health Effects.
[4] Brunekreef, B. and Holgate, S.T. (2002) Air Pollution and Health. The Lancet, 360, 1233-1242.
[5] Review of Evidence on Health Aspects of Air Pollution—Revihaap Project (2003) World Health Organization Regional Office for Europe, Danmark.
[6] Rackley, C.R. and Stripp, B.R. (2012) Building and Maintaining the Epithelium of the Lung. Journal of Clinical Investigation, 122, 2724-2730.
[7] Wargo, J., Wargo, L. and Alderman, N. (2006) The Harmful Effects of Vehicle Exhaust: A Case for Policy Change. Environment and Human Health, Inc., North Haven.
[8] Green, G.M., Jakab, G.J., Low, R.B. and Davis, G.S. (1977) Defense Mechanisms of the Respiratory Membrane. The American Review of Respiratory Disease, 115, 479-514.
[9] Tamura, S. and Kurata, T. (2004) Defense Mechanisms against Influenza Virus Infection in the Respiratory Tract Mucosa. Japanese Journal of Infectious Disease, 57, 236-247.
[10] Ma, W.J., Sun, Y.H., Jiang, J.X., Dong, X.W., Zhou, J.Y. and Xie, Q.M. (2014) Epoxyeicosatrienoic Acids Attenuate Cigarette Smoke Extract-Induced Interleukin-8 Production in Bronchial Epithelial Cells. Prostaglandins Leukotrienes and Essential Fatty Acids.
[11] Pessina, G.P., Paulesu, L., Corradeschi, F., Luzzi, E., Stefano, A.D., Tanzini, M., Matteucci, G. and Bocci, V. (1993) Effects of Acute Cigarette Smoke Exposure on Macrophage Kinetics and Release of Tumour Necrosis Factor Alpha in Rats. Mediators of Inflammation, 2, 119-122.
[12] Finch, G.L., Nikula, K.J., Chen, B.T., Barr, E.B., Chang, I. and Hobbs, C.H. (2002) Effect of Chronic Cigarette Smoke Exposure on Lung Clearance of Tracer Particles Inhaled by Rats. Fundamental and Applied Toxicology, 24, 76-85.
[13] Sekine, T., Sakaguchi, C. and Fukano, Y. (2014) Investigation by Microarray Analysis of Effects of Cigarette Design Characteristics on Gene Expression in Human Lung Mucoepidermoid Cancer Cells NCI-H292 Exposed to Cigarette Smoke. Experimental and Toxicological Pathology, 67, 143-151.
[14] Liu, M.H., Lin, A.H., Lu, S.H., Peng, R.Y., Lee, T.S. and Kou, Y.R. (2014) Eicosapentaenoic Acid Attenuates Cigarette Smoke-Induced Lung Inflammation by Inhibiting ROS-Sensitive Inflammatory Signaling. Frontiers in Physiology, 5, 440.
[15] Phipps, J.C., Aronoff, D.M., Curtis, J.L., Goel, D., O’Brien, E. and Mancuso, P. (2010) Cigarette Smoke Exposure Impairs Pulmonary Bacterial Clearance and Alveolar Macrophage Complement-Mediated Phagocytosis of Streptococcus pneumonia. Infection and Immunity, 78, 1214-1220.
[16] Kirkham, P.A., Spooner, G., Ffoulkes-Jones, C. and Calvez, R. (2003) Cigarette Smoke Triggers Macrophage Adhesion and Activation: Role of Lipid Peroxidation Products and Sca-venger Receptor. Free Radical Biology and Medicine, 35, 697-710.
[17] van der Vaart, H., Postma, D.S., Timens, W. and ten Hacken, N.H. (2004) Acute Effects of Cigarette Smoke on Inflammation and Oxidative Stress: A Review. Thorax, 59, 713-721.
[18] Liu, M.-H., Lin, A.-H., Lee, H.-F., Ko, H.-K., Lee, T.-S. and Kou, Y.R. (2014) Paeonol Attenuates Cigarette Smoke-Induced Lung Inflammation by Inhibiting ROS-Sensitive Inflammatory Signaling. Mediators of Inflammation, 2014, Article ID: 651890.
[19] Churg, A., Dai, J., Tai, H., Xie, C. and Wright, J.L. (2002) Tumor Necrosis Factor-Alpha Is Central to Acute Cigarette Smoke-Induced Inflammation and Connective Tissue Breakdown. American Journal of Respiratory and Critical Care Medicine, 166, 849-854.
[20] Churg, A., Zay, K., Shay, S., Xie, C., Shapiro, S.D., Hendricks, R. and Wright, J.L. (2002) Acute Cigarette Smoke-Induced Connective Tissue Breakdown Requires Both Neutrophils and Macrophage Metalloelastase in Mice. American Journal of Respiratory Cell and Molecular Biology, 27, 368-374.
[21] Karimi, K., Sarir, H., Mortaz, E., Smit, J.J., Hosseini, H., De Kimpe, S.J., Nijkamp, F.P. and Folkerts, G. (2006) Toll-Like Receptor-4 Mediates Cigarette Smoke-Induced Cytokine Production by Human Macrophages. Respiratory Research, 19, 66.
[22] Li, J., Zhou, W., Huang, K., Jin, Y. and Gao, J. (2014) Interleukin-22 Exacerbates Airway Inflammation Induced by Short-Term Exposure to Cigarette Smoke in Mice. Acta Pharmacologica Sinica, 35, 1393-1401.
[23] Guo, Z. and Jiang, S. (2004) Pharmacological Activities and Therapeutic Applications of Lily bulbs. Acta Chinese Medicine and Pharmacology, 32, 27-29.
[24] Kwon, O.K., Lee, M.Y., Yuk, J.E., Oh, S.R., Chin, Y.W., Lee, H.K. and Ahn, K.S. (2010) Anti-Inflammatory Effects of Methanol Extracts of the Root of Lilium lancifolium on LPS-Stimulated Raw264.7 Cells. Journal of Ethnopharmacology, 130, 28-34.
[25] Lee, E., Yun, N., Jang, Y.P. and Kim, J. (2013) Lilium lancifolium Thunb. Extract Attenuates Pulmonary Inflammation and Air Space Enlargement in a Cigarette Smoke-Exposed Mouse Model. Journal of Ethnopharmacology, 149, 148-156.
[26] Hwang, Y., Yang, M. and Pyo, M. (2008) Effects of Heated Pear Juice on the Proliferation and Cytokines Production of Mouse Splenocytes in Vitro. Journal of Cancer Prevention, 13, 68-73.
[27] Lee, Y.G., Cho, J.Y., Kim, C., Lee, S., Kim, W., Jeon, T., Park, K. and Moon, J. (2013) Coumaroyl Quinic Acid Derivatives and Flavonoids from Immature Pear (Pyrus pyrifolia Nakai) Fruit. Food Science and Biotechnology, 22, 803-810.
[28] Zhang, H. and Li, X.H. (2011) Research Advance of Pharmacological Effects and Toxicity of Siraitia grovenorii (Swingle) C. Jeffrey. Chinese Agricultural Science Bulletin, 27, 430-433.
[29] Wang, D. (2002) [Pharmacological and Clinical Applications of Apricot kernel]. Primary Journal of Chinese Materia Medica, 16, 61-62.
[30] Wang, X. (2009) Therapeutic Applications of Lily bulbs. Aerospace Medicine, 19, 151.
[31] Coppi, G. and Gatti, M.T. (1989) A Method for Studying Expectorant Action in the Mouse by Measurement of Tracheobronchial Phenol Red Secretion. Farmaco, 44, 541-545.
[32] Knowles, M.R. and Boucher, R.C. (2002) Mucus Clearance as a Primary Innate Defense Mechanism for Mammalian Airways. The Journal of Clinical Investigation, 109, 571-577.
[33] Canning, B.J. (2010) Afferent Nerves Regulating the Cough Reflex: Mechanisms and Mediators of Cough in Disease. Otolaryngologic Clinics of North America, 43, 15-25.
[34] Zheng, F., Liu, W., Zheng, Y. and Li, W. (2011) Comparation of Expectorant Effect of Total Phatycosides and Secondary Saponin from the Roots of Platycodon grandiflorum. Journal of Jilin Agricultural University, 7.
[35] Zhao, S.G. and Wang, L. (2009) Investigation of the Effects of Acidic Extracts from Ganoderma lucidum Fermentation Broth on Chronic Bronchitis. Mycosystema, 28, 832-837.
[36] Zou, L., Liang, C., Qiu, F. and Tao, X. (2002) Antitussive and Antiasthmatic Action of Wu-Hu-Tang. Chinese Journal of Experimental Traditional Medical Fromulae, 8, 38-39.
[37] Zhang, D. (2011) Methods and Evalu-ation of Animal Models of Induced Cough. Acta Neuropharmacologica, 1, 35-42.
[38] Angrill, J., Agusti, C., De Celis, R., Filella, X., Rañó, A., Elena, M., De La Bellacasa, J.P., Xaubet, A. and Torres, A. (2001) Bronchial Inflammation and Colonization in Patients with Clinically Stable Bronchiectasis. American Journal of Respiratory and Critical Care Medicine, 164, 1628-1632.
[39] Uren, T.K., Johansen, F.E., Wijburg, O.L., Koentgen, F., Brandtzaeg, P. and Strugnell, R.A. (2003) Role of the Polymeric Ig Receptor in Mucosal B Cell Homeostasis. The Journal of Immunology, 170, 2531-2539.
[40] Moghaddam, A.S., Shabani, M., Fateminasab, F. and Khakzad, M.R. (2005) Level of Nitric Oxide in Bronchoalveolar Lavage Fluid of Asthmatic mice Model. Iranian Journal of Immunology, 2, 103-110.
[41] Goncalves, C.T.R., Goncalves, C.G.R., de Almeida, R.M., Lopes, F.M., dos Santos Durao, A.C., dos Santos, F.A., da Silva, L.F., Marcourakis, T., Castro-Faria-Neto, H.C., Vieira Rde, P. and Dolhnihkff, M. (2012) Protective Effects of Aerobic Exercise on Acute Lung Injury Induced by LPS in Mice. Critical Care, 16, R199-R209.
[42] Li, W., Deng, G.C., Li, M., Liu, X.M. and Wang, Y.J. (2012) Roles of Mucosal Immunity against Mycobacterium tuberculosis Infection. Tuberculosis Research and Treatment, 2012, Article ID: 791728.
[43] Mushaben, E.M., Brandt, E.B., Khurana Hershey, G.K. and Le Cras, T.D. (2013) Differential Effects of Rapamycin and Dexamethasone in Mouse Models of Established Allergic Asthma. PLoS ONE, 8, e54426.
[44] Chandler, J.D., Min, E., Huang, J., Nichols, D.P. and Day, B.J. (2013) Nebulized Thiocyanate Improves Lung Infection Outcomes in Mice. British Journal of Pharmacology, 169, 1166-1177.
[45] Okada, S., Hasegawa, S., Hasegawa, H., Ainai, A., Atsuta, R., Ikemoto, K., Sasaki, K., Toda, S., Shirabe, K., Takahara, M., Harada, S., Morishima, T. and Ichiyama, T. (2013) Analysis of Bronchoalveolar Lavage Fluid in a Mouse Model of Bronchial Asthma and H1N1 2009 Infection. Cytokine, 63, 194-200.
[46] Rubin, B.K. (2007) Mucolytics, Expectorants, and Mucokinetic Medications. Respiratory Care, 52, 859-865.
[47] Rubin, B.K. (2014) Secretion Properties, Clearance, and Therapy in Airway Disease. Translational Respiratory Medicine, 2, 6.
[48] De Blasio, F., Virchow, J.C., Polverino, M., Zanasi, A., Behrakis, P.K., Kilinç, G., Balsamo, R., De Danieli, G. and Lanata, L. (2007) Cough Management: A Practical Approach. Cough, 7, 7.
[49] Chung, K.F. (2011) Chronic “Cough Hypersensitivity Syndrome”: A More Precise Label for Chronic Cough. Pulmonary Pharmacology and Therapeutics, 24, 267-271.
[50] Yousaf, N., Montinero, W., Birring, S.S. and Pavord, I.D. (2013) The Long Term Outcome of Patients with Unexplained Chronic Cough. Respiratory Medicine, 107, 408-412.
[51] Ryan, N.M. and Gibson, P.G. (2014) Recent Additions in the Treatment of Cough. Journal of Thoracic Disease, 6, S739-S747.
[52] Dicpinigaitis, P.V., Canning, B.J., Garner, R. and Paterson, B. (2015) Effect of Memantine on Cough Reflex Sensitivity: Translational Studies in Guinea Pigs and Humans. Journal of Pharmacology and Experimental Therapeutics, 352, 448-454.
[53] Janeway, C.A., Travers, P., Walport, M. and Shlomchik, M.J. (2001) Immunobiology: The Immune System in Health and Disease. Grand Science, New York.
[54] Aoshiba, K., Tamaoki, J. and Nagai, A. (2001) Acute Cigarette Smoke Exposure Induces Apoptosis of Alveolar Macrophages. American Journal of Physiology: Lung Cellular and Molecular Physiology, 281, L1392-L1401.
[55] van der Vaart, H., Postma, D.S., Timens, W. and Ten Hacken, N.H.T. (2004) Acute Effects of Cigarette Smoke on Inflammation and Oxidative Stress: A Review. Thorax, 59, 713-721.
[56] Goldman, A.S. and Prabhakar, B.S. (1996) Medical Microbiology. University of Texas Medical Branch, Galveston.

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