Effect of Ribose on Mature/Immature Raw Peanut Proteins and Their Allergenic Properties
Si-Yin Chung
DOI: 10.4236/fns.2011.24042   PDF   HTML   XML   5,747 Downloads   9,269 Views   Citations


Mature and immature roasted peanuts are reportedly different in the level of Maillard reaction adducts (MRA) and IgE binding (i.e., allergenic capacity). Heating and sugar-protein interaction are the cause for the difference. The objective of this study was to determine if mature and immature raw peanuts (not roasted) are also different through treatment with a reducing sugar such as ribose, glucose or fructose at a mild temperature. Extracts from mature and immature raw peanuts were treated with individual sugars at 37?C and 50?C, respectively, for 0 - 10 days, and then assayed for MRA with nitroblue tetrazolium (NBT) in a time-course manner for 60 min. IgE binding was determined in an enzyme- linked immunosorbent assay (ELISA), using a pooled plasma from peanut-allergic individuals. Of the sugars tested, only ribose produced a big difference or a unique curve pattern in MRA between treated mature and immature peanuts. The unique curve pattern was more pronounced at 50?C (day 5 - 10) than at 37?C. IgE binding under this condition increased, but only with the ribose-treated mature raw peanut. It was concluded that mature and immature raw peanuts were different in MRA and IgE binding when treated with ribose only, and that under such a condition, mature and immature raw peanuts could be identified.

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S. Chung, "Effect of Ribose on Mature/Immature Raw Peanut Proteins and Their Allergenic Properties," Food and Nutrition Sciences, Vol. 2 No. 4, 2011, pp. 294-300. doi: 10.4236/fns.2011.24042.

Conflicts of Interest

The authors declare no conflicts of interest.


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