Purple Grape Juice, an Important Flavonoids Source, Influence in Biochemical Parameters in Offspring of Wistar Rats


Our objective was to evaluate the effect of chronic gestational treatment and post-breast feeding with purple grape juice on the biochemical parameters in offspring of dams. We used 40 male offspring from 14 Wistar rats which received purple grape juice or water by gavage daily for 42 days at a dose of 7 microliters/g. The offspring were divided into groups of water or juice. All received daily purple grape juice or water by gavage for 30 days after completion of their nursing period at a dose of 7 microliters/g. At the end of the treatment period, the offspring were euthanized by decapitation with its truncal blood collected and subsequently separated the serum from the blood. The measurements were performed by automation or by manual dosing. Considering the gestational factor, grape juice was able to reduce levels of total cholesterol, triglycerides, urea, alanine aminotransferase (ALT), and not alter the levels of creatinine and aspartate aminotransferase (AST) in serum of the offspring. When considering the post-breast feeding factor, grape juice resulted in an improvement in HDL-C. We concluded that purple grape juice was able to produce a beneficial effect on certain biochemical parameters in offspring.

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Hilger, D. , Wohlenberg, M. , Schaffer, T. , Machado, F. , Gonçalves, L. , Bortolato, G. , Dani, G. , Rodrigues, A. , Funchal, C. and Dani, C. (2015) Purple Grape Juice, an Important Flavonoids Source, Influence in Biochemical Parameters in Offspring of Wistar Rats. Food and Nutrition Sciences, 6, 683-691. doi: 10.4236/fns.2015.67071.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Dani, C., Oliboni, L.S., Bonatto, D., Vanderlinde, R., Salvador, M. and Henriques, J.A. (2007) Phenolic Content and Antioxidant Activities of White and Purple Juices Manufactured with Organically-Produced Grapes. Food and Chemical Toxicology, 45, 2574-2580.
[2] Miyagi, Y., Miwa, K. and Inoue, H. (1997) Inhibition of Human Low Density Lipoprotein Oxidation by Flavonoids in Red Wine and Grape Juice. American Journal of Cardiology, 80, 1627-1631.
[3] Mazza, G. (1995) Anthocyanins in Grapes and Grape Products. Critical Reviews in Food Science and Nutrition, 35, 341-371.
[4] Oak, M.H., ElBedoui, J. and Schini-Kerth, V.B. (2005) Antiangiogenic Properties of Natural Polyphenols from Red Wine and Green Tea. The Journal of Nutritional Biochemistry, 16, 1-8.
[5] Belguendouz, F.L. and Gozzelino, M.T. (1998) Interaction of Transresveratrol with Plasma Lipoproteins. Biochemical Pharmacology, 15, 811-816.
[6] Castilla, P., Echarri, R., Da’valos, A., Cerrato, F., Ortega, H., Teruel, J., Lucas, M., Gómez-Coronado, D., Ortuño, J. and Lasunción, M. (2006) Concentrated Red Grape Juice Exerts Antioxidant, Hypolipidemic, and Antiinflammatory Effects in Both Hemodialysis Patients and Healthy Subjects. The American Journal of Clinical Nutrition, 84, 252-262.
[7] Stein, J.H., Keevil, J.G., Wiebe, D.A., Aeschlimann, S. and Folts, J.D. (1999) Purple Grape Juice Improves Endothelial Function and Reduces the Susceptibility of LDL Cholesterol to Oxidation in Patients with Coronary Artery Disease. Circulation, 100, 1050-1055.
[8] Zibaeenezhad, M., Mohammadi, E., Beigi, M.A., Mirzamohammadi, F. and Salehi, O. (2012) The Effects of Unripe Grape Juice on Lipid Profile Improvement. Cholesterol, 2012, Article ID: 890262.
[9] Dani, C., Oliboni, L.S., Umezu, F.M., Pasquali, M., Salvador, M., Moreira, J.C. and Henriques, J.A. (2009) Antioxidant and Antigenotoxic Activities of Purple Grape Juice—Organic and Conventional—In Adult Rats. Journal of Medicinal Food, 12, 1111-1118.
[10] Agarwal, A. and Allamaneni, S.S. (2004) Role of Free Radicals in Female Reproductive Diseases and Assisted Reproduction. Reproductive BioMedicine Online, 9, 338-347.
[11] Agarwal, A., Gupta, S. and Sikka, S. (2006) The Role of Free Radicals and Antioxidants in Reproduction. Current Opinion in Obstetrics and Gynecology, 18, 325-332.
[12] Vannuchi, C.I., Jordao, A.A. and Vannuchi, H. (2007) Antioxidant Compounds and Oxidative Stress in Female Dogs during Pregnancy. Research in Veterinary Science, 83, 188-193.
[13] Arola-arnal, A., Oms-Oliu, G., Crescenti, A., Del Bas, J.M., Ras, M.R., Arola, L. and Caimari, A. (2013) Distribution of Grape Seed Flavanols and Their Metabolites in Pregnant Rats and Their Fetuses. Molecular Nutrition & Food Research, 57, 1741-1752.
[14] Zielinsky, P., Piccoli, A.L., Manica, J.L.L. and Nicoloso, L.H. (2010) New Insights on Fetal Ductal Constriction: Role of Maternal Ingestion of Polyphenol-Rich Foods. Expert Review of Cardiovascular Therapy, 8, 291-298.
[15] Zoecklein, B.W., Fugelsang, K.C., Gump, B.H. and Nury, F.S. (2000) Wine Analysis and Production. Aspen Publishers, Maryland.
[16] Singleton, V.L., Orthofer, R. and Lamuela-Ravento’s, R.M. (1999) Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteau Reagent. Methods in Enzymology, 299, 152-178.
[17] Park, Y.K., Park, E., Kim, J. and Kang, M. (2003) Daily Grape Juice Consumption Reduces Oxidative DNA Damage and Plasma Free Radical Levels in Healthy Koreans. Mutation Research, 529, 77-86.
[18] Dani, C., Pasquali, M.A., Oliveira, M.R., Umezu, F.M., Salvador, M., Henriques, J.á. and Moreira, J.C. (2008) Protective Effects of Purple Grape Juice on Carbon Tetrachloride-Induced Oxidative Stress in Brains of Adult Wistar Rats. Journal of Medicinal Food, 11, 55-61.
[19] Dani, C., Oliboni, L.S., Pasquali, M.A., Oliveira, M.R., Umezu, F.M., Salvador, M., Moreira, J.C. and Henriques, J.A. (2008) Intake of Purple Grape Juice as a Hepatoprotective Agent in Wistar Rats. Journal of Medicinal Food, 11, 127-132.
[20] Ferguson, L.R. (2001) Role of Plant Polyphenols in Genomic Stability. Mutation Research, 475, 89-111.
[21] Shahidi, F. and Wanasundara, P.K. (1992) Phenolic Antioxidants. Critical Reviews in Food Science and Nutrition, 32, 67-103.
[22] Al-gubory, K.H., Fowler, P.A. and Garrel, C. (2010) The Roles of Cellular Reactive Oxygen Species, Oxidative Stress and Antioxidants in Pregnancy Outcomes. The International Journal of Biochemistry & Cell Biology, 10, 1634-1650.
[23] de Bem, G.F., da Costa, C.A., de Oliveira, P.R., Cordeiro, V.S., Santos, I.B., de Carvalho, L.C., Souza, M.A., Ognibene, D.T., Daleprane, J.B., Sousa, P.J., Resende, A.C. and de Moura, R.S. (2014) Protective Effect of Euterpeoleracea Mart (A?aí) Extract on Programmed Changes in the Adult Rat Offspring Caused by Maternal Protein Restriction during Pregnancy. Journal of Pharmacology and Pharmacotherapeutics, 66, 1328-1338.
[24] Aderimi, F.A. (2004) Effects of Replacement of Wheat Bran with Cassava Root Sieviate Supplemented or Unsupplemented with Enzyme on Hematology and Serum Biochemistry of Pulled Chicks. Journal of Tropical Forest Science, 7, 147-153.
[25] Hort, M.A., Schuldt, E.Z., Bet, A.C., DalBó, S., Siqueira, J.M., Ianssen, C., Abatepaulo, F., de Souza, H.P., Veleirinho, B., Maraschin, M. and Ribeiro-do-Valle, R.M. (2012) Anti-Atherogenic Effects of a Phenol-Rich Fraction from Brazilian Red Wine (Vitis labrusca L.) in Hypercholesterolemic Low-Density Lipoprotein Receptor Knockout Mice. Journal of Medicinal Food, 15, 936-44.
[26] Vinson, J.A., Teufel, K. and Wu, N. (2001) Red Wine, Dealcoholized Red Wine, and Especially Grape Juice, Inhibit Atherosclerosis in a Hamster Model. Atherosclerosis, 156, 67-72.
[27] Perez-Jimenez, J. and Saura-Calixto, F. (2008) Grape Products and Cardiovascular Disease Risk Factors. Nutrition Research Reviews, 21, 158-173.
[28] Khadem-Ansari, M., Rasmi,Y. and Ramezani, F. (2010) Effects of Red Grape Juice Consumption on High Density Lipoprotein-Cholesterol, Apolipoprotein AI, Apolipoprotein B and Homocysteine in Healthy Human Volunteers. The Open Biochemistry Journal, 4, 96-99.
[29] Pinheiro, F.V., Pimentel, V.C., De Bona, K.S., Scola, G., Salvador, M., Funchal, C. and Moretto, M.B. (2010) Decrease of Adenosine Deaminase Activity and Increase of the Lipid Peroxidation after Acute Methotrexate Treatment in Young Rats: Protective Effects of Grape Seed Extract. Cell Biochemistry and Function, 28, 89-94.
[30] Safa, J., Argani, H., Bastani, B., Nezami, N., RahimiArdebili, B., Ghorbanihaghjo, A., Kalagheichi, H., Amirfirouzi, A., Mesgari, M. and Soleimany Rad, J. (2010) Protective Effect of Grape Seed Extract on Gentamicin-Induced Acute Kidney Injury. Iranian Journal of Kidney Diseases, 4, 285-291.
[31] Ahmad, F. and Khan, G.M. (2012) Study of Aging and Hepatoprotective Activity of Vitis vinifera L. Seeds in Albino Rats. Asian Pacific Journal of Tropical Biomedicine, 2, S1770-S1774.
[32] Orhan, D.D., Orhan, N., Ergun, E. and Ergun, F. (2007) Hepatoprotective Effect of Vitis vinifera L. Leaves on Carbon Tetrachloride-Induced Acute Liver Damage in Rats. Journal of Ethnopharmacology, 112, 145-151.
[33] Alimi, H., Hfaeidh, N., Mbarki, S., Bouoni, Z., Sakly, M. and Rouma, K.B. (2012) Evaluation of Opuntia ficus indica F. Inermis Fruit Juice Hepatoprotective Effect upon Ethanol Toxicity in Rats. General Physiology and Biophysics, 31, 335-342.

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