Induced Monocytes-Derived HSCs (CD34+) with LPS Accelerated Homing Rat Bone Marrow-Mesenchymal Stem Cell (BM-MSCs, CD105) in Injured Pancreas

Fedik A. Rantam^1,2*,   Purwati^1,3, Budi Setiawan⁴, Sony Wibisono³,   Ferdiansyah^1,5, Joni Wahyuhadi^1,6, Edward Mouli^1,5, Dwikora N. Utomo^1,5, Heri Suroto^1,5, Candra Bumi¹

¹Stem Cell Laboratory, Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia.
²Virology and Immunology Laboratory, Department of Microbiology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.
³Tropic and Infection Division of Internal Medicine, School of Medicine, Airlangga University, Surabaya, Indonesia.
⁴Animal Surgery, Animal Hospital, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.
⁵Regenerative Medicine, Department of Orthopedic, Dr. Soetomo Teaching Hospital/School of Medicine, Airlangga University, Surabaya, Indonesia.
⁶Department of Neurosurgery, Dr. Soetomo Teaching Hospital/School of Medicine, Airlangga University, Surabaya, Indonesia.

DOI: 10.4236/jbise.2015.85031                

Abstract

Investigating the function of combining induced rat monocytes-derived bone marrow-haemopoietic stem cell (rat BM-HSCs) with LPS and rat bone marrow-mesenchymal stem cell (rat BM-MSCs) was to analyze the acceleration of homing process mechanism in injured pancreas. Mononucleated stem cells were isolated from aspirated whole rat BM using ficoll and cultured in α-MEM complete growth medium in 10 cm petridish. After two days, adherent cells after washing twice in petridish were added α-MEM growth medium and then mesenchymal cells were characterized using CD105 marker in third passage and labeled PKH26. Then haemopoietic stem cells (HSCs) were isolated with magnetic beads CD34+ and differentiated in vitro, and then induced monocytes with LPS. Animal experiment used 28 male Wistar rats, and divided them into 4 groups. After transplantation combined, both cells between monocyte derived HSc (mHSCs) and rat BM-MSC were analyzed expression of pair box gen 4 (Pax4), pancreatic and duodenal homeobox (Pdx1), C-peptide using immunohistochemistry, then secretion of insulin and C-peptide analyzed using indirect ELISA. Results showed that the expressions of Pax4, Pdx1, C-peptide found in the surface membrane cell of pancreatic cell, and secreted C-peptide and insulin were shown significant (P < 0.05) in transplanted group 2, 3 and 4, but in group 3 were transplanted with combined cells more dominant than non-combined cells. Conclusions suggested that combining of induced monocytes-derived HSCs and rat BM-MSCs has accelerated homing MSCs into injured pancreatic tissue.

Keywords

Induced Monocyte Derived HSCs, Rat BM-MSCs, Homing, Injured Pancreas

Conflicts of Interest

The authors declare no conflicts of interest.

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