Quantitation of the Total Catechin Content in Oils Extracted from Seeds of Selected Tea (Camellia sinensis (L) O. Kuntze, Theaceae) Clones by RP-HPLC

Kelvin Omondi George^1,2, Kelvin Okong’o Moseti^2,3*, John Kanyiri Wanyoko³, Thomas Kinyanjui², Francis Nyamu Wachira⁴

¹Nairobi Bottlers Limited, Nairobi, Kenya.
²Chemistry Department, Egerton University, Nakuru, Kenya.
³Tea Research Institute, Kericho, Kenya.
⁴Association for Strengthening Agricultural Research in Eastern and Central Africa, Entebbe, Uganda.

DOI: 10.4236/ajps.2015.67112                

Abstract

Catechins (flavan-3-ols) are polyphenolic plant secondary metabolites that have been strongly associated with a wide variety of beneficial health effects in vitro, in vivo and clinically. This study reports findings on the content of catechins in tea seed oil (TSO) extracted by Soxhlet extraction from seeds of different clones of Kenyan tea. Extraction of catechins from the crude oils was achieved by sequential liquid-liquid extraction (LLE) using methanol and quantified by reverse phase High Performance Liquid Chromatography (RP-HPLC). Results obtained revealed that all the crude test oils contained catechins, with oils extracted from clones TRFK K-Purple and GW-Ejulu having the highest total catechin content of 9.8 ± 0.25 and 9.0 ± 0.83 (×10^-3% flavonoids) respectively. Statistically significant differences (p < 0.05) were evident in the total catechin contents of crude oils extracted from tea seeds with those extracted from corn, sunflower and soybean seeds. Moreover, clonal variations were evident, as the total catechin contents of oils extracted from clones TRFK K-Purple and GW-Ejulu were statistically different (p < 0.05) from those extracted from clones TRFK 301/3, TRFK 301/4, TRFK 301/5, TRFK 306, TRFK 91/1 and TRFCA SFS 150. Thus, the current findings strongly suggest that oils from seeds of Kenyan tea cultivars can be a potential source of potent natural antioxidants.

Keywords

Soxhlet Extraction, TSO, RP-HPLC, Catechins, LLE

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Sahari, M. and Amooi, M. (2013) Tea Seed Oil: Extraction, Compositions, Applications, Functional and Antioxidant Properties. Academia Journal of Medicinal Plants, 1, 68-79.
[2]	Hilal, Y. and Engelhardt, U. (2007) Characterisation of White Tea—Comparison to Green and Black Tea. Journal of Consumer Protection and Food Safety, 2, 414-421.
[3]	Rusak, G., Komes, D., Likic, S., Horzic, D. and Kovac, M. (2008) Phenolic Content and Antioxidative Capacity of Green and White Tea Extracts Depending on Extraction Conditions and the Solvent Used. Food Chemistry, 110, 852-858. http://dx.doi.org/10.1016/j.foodchem.2008.02.072
[4]	Sang, S., Lambert, J.D., Ho, C.T. and Yang, C.S. (2011) The Chemistry and Biotransformation of Tea Constituents. Pharmacological Research, 64, 87-99. http://dx.doi.org/10.1016/j.phrs.2011.02.007
[5]	Coe, S., Fraser, A. and Ryan, L. (2013) Polyphenol Bioaccessibility and Sugar Reducing Capacity of Black, Green, and White Teas. International Journal of Food Science, 2013, Article ID: 238216.
[6]	George, K.O., Kinyanjui, T., Wanyoko, J., Moseti, O.K. and Wachira, F. (2013) Extraction and Analysis of Tea (Camellia sinensis) Seed Oil from Different Clones in Kenya. African Journal of Biotechnology, 12, 841-846.
[7]	Yahaya, L.E., Adebowale, K.O., Olu-Owolabi, B.I. and Menon, A.R.R. (2011) Compositional Analysis of Tea (Camellia sinensis) Seed Oil and Its Application. International Journal of Research in Chemistry and Environment, 1, 153-158.
[8]	Wang, Y., Sun, D., Chen, H., Qian, L. and Xu, P. (2011) Fatty Acid Composition and Antioxidant Activity of Tea (Camellia sinensis L.) Seed Oil Extracted by Optimized Supercritical Carbon Dioxide. International Journal of Molecular Science, 12, 7708-7719. http://dx.doi.org/10.3390/ijms12117708
[9]	Njuguna, D.G., Wanyoko, J.K., Kinyanjui, T. and Wachira, F.N. (2013) Mineral Elements in the Kenyan Tea Seed Oil Cake. International Journal of Research in Chemistry and Environment, 3, 253-261.
[10]	Njuguna, D.G., Wanyoko, J.K., Kinyanjui, T. and Wachira, F.N. (2013) Polyphenols and Free Radical Scavenging Properties of Kenyan Tea Seed Oil Cake. International Journal of Research in Chemistry and Environment, 3, 86-92.
[11]	Njuguna, D.G., Wanyoko, J.K., Kinyanjui, T. and Wachira, F.N. (2013) Fatty Acids Residues Composition in the De-Oiled Tea Seed Oil Cakes. Science Journal of Biotechnology, 2014, Article ID: sjbt-263.
[12]	Yang, Z., Xu, Y., Jie, G., He, P. and Tu, Y. (2007) Study on the Antioxidant Activity of Tea Flowers (Camellia sinensis). Asia Pacific Journal of Clinical Nutrition, 16, 148-152.
[13]	Chen, B.T., Li, W.X., He, R.R., Li, Y.F., Tsoi, B., Zhai, Y.J. and Kurihara, H. (2012) Anti-Inflammatory Effects of a Polyphenols-Rich Extract from Tea (Camellia sinensis) Flowers in Acute and Chronic Mice Models. Oxidative Medicine and Cellular Longevity, 2012, Article ID: 537923. http://dx.doi.org/10.1155/2012/537923
[14]	Zhang, X., Gao, Y., Xu, J., Liu, X., Jin, F., Li, B. and Tu, Y. (2014) Inhibitory Effect of Tea (Camellia sinensis (L.) O. Kuntze, Theaceae) Flower Extracts on Oleic Acid-Induced Hepatic Steatosis in Hepg2 Cells. Journal of Food and Nutrition Research, 2,738-743. http://dx.doi.org/10.12691/jfnr-2-10-13
[15]	Vuong, Q.V. (2014) Epidemiological Evidence Linking Tea Consumption to Human Health: A Review. Critical Reviews in Food Science and Nutrition, 54, 523-536. http://dx.doi.org/10.1080/10408398.2011.594184
[16]	Khan, N. and Mukhtar, H. (2007) Tea Polyphenols for Health Promotion. Life Sciences, 81, 519-533. http://dx.doi.org/10.1016/j.lfs.2007.06.011
[17]	Singh, V., Verma, D.K. and Singh, G. (2014) Processing Technology and Health Benefits of Green Tea. Popular Kheti, 2, 23-30.
[18]	Karak, T. and Bhagat, R.M. (2010) Trace Elements in Tea Leaves, Made Tea and Tea Infusion: A Review. Food Research International, 43, 2234-2252. http://dx.doi.org/10.1016/j.foodres.2010.08.010
[19]	Zuo, Y., Chen, H. and Deng, Y. (2002) Simultaneous Determination of Catechins, Caffeine and Gallic Acids in Green, Oolong, Black and Pu-Erh Teas Using HPLC with a Photodiode Array Detector. Talanta, 57, 307-316. http://dx.doi.org/10.1016/S0039-9140(02)00030-9
[20]	Chung, F., Schwrtz, J., Herzog, C.R. and Yang, Y. (2003) Tea and Cancer Prevention: Studies in Animals and Humans. Journal of Nutrition, 133, 3268S-3274S.
[21]	Yang, C.S., Ju, J., Lu, G., Xiao, H., Hao, X., Sang, S. and Lambert, J.D. (2008) Cancer Prevention by Tea and Tea Polyphenols. Asia Pacific Journal of Clinical Nutrition, 17, 245-248.
[22]	Yang, C.S., Lambert, J.D., Ju, J., Lu, G. and Sang, S. (2007) Tea and Cancer Prevention: Molecular Mechanisms and Human Relevance. Toxicology and Applied Pharmacology, 224, 265-273. http://dx.doi.org/10.1016/j.taap.2006.11.024
[23]	Karori, S.M., Wachira, F.N., Wanyoko, J.K. and Ngure, R.M. (2007) Antioxidant Capacity of Different Types of Tea Products. African Journal of Biotechnology, 6, 2287-2296.
[24]	Koech, R.K., Wanyoko, J. and Wachira, F. (2014) Antioxidant, Antimicrobial and Synergistic Activities of Tea Polyphenols. International Journal of Infectious Diseases, 21, 98. http://dx.doi.org/10.1016/j.ijid.2014.03.631
[25]	Karori, S.M., Ngure, R.M., Wachira, F.N., Wanyoko, J.K. and Mwangi, J.N. (2008) Different Types of Tea Products Attenuate Inflammation Induced in Trypanosoma brucei Infected Mice. Parasitology International, 57, 325-333. http://dx.doi.org/10.1016/j.parint.2008.02.003
[26]	Al-Attar, A.M. and Zari, T.A. (2010) Influences of Crude Extract of Tea Leaves, Camellia sinensis, on Streptozotocin Diabetic Male Albino Mice. Saudi Journal of Biological Sciences, 17, 295-301. http://dx.doi.org/10.1016/j.sjbs.2010.05.007
[27]	Elshater, A.A., Salman, M.A. and Moussa, M.M.A. (2008) Effect of Green Tea Consumption on Level of Glucose, Lipid Profile and Kidney Functions in Alloxan Induced-Diabetic Rats. Egyptian Academic Journal of Biological Sciences, 1, 125-134.
[28]	Tsuneki, H., Ishizuka, M., Terasawa, M., Wu, J.B., Sasaoka, T. and Kimura, I. (2004) Effect of Green Tea on Blood Glucose Levels and Serum Proteomic Patterns in Diabetic (db/db) Mice and on Glucose Metabolism in Healthy Humans. BMC Pharmacology, 4, 18. http://dx.doi.org/10.1186/1471-2210-4-18
[29]	Odegaard, A.O., Pereira, M.A., Koh, W.P., Arakawa, K., Lee, H.P. and Yu, M.C. (2008) Coffee, Tea, and Incident Type 2 Diabetes: The Singapore Chinese Health Study. American Journal of Clinical Nutrition, 88, 979-985.
[30]	Jing, Y., Han, G., Hu, Y., Bi, Y., Li, L. and Zhu, D. (2009) Tea Consumption and Risk of Type 2 Diabetes: A Meta-Analysis of Cohort Studies. Journal of General Internal Medicine, 24, 557-562. http://dx.doi.org/10.1007/s11606-009-0929-5
[31]	Koech, K.R., Wachira, F.N., Ngure, R.M., Orina, I.A., Wanyoko, J.K., Bii, C. and Karori, S.M. (2013) Antifungal Activity of Crude Tea Extracts. African Journal of Agricultural Research, 8, 2086-2089.
[32]	Koech, K.R., Wachira, F.N., Ngure, R.M., Wanyoko, J.K., Bii, C. and Karori, S.M. (2013) Antibacterial and Synergistic Activity of Different Tea Crude Extracts Against Antibiotic Resistant S. aureus, E. coli and a Clinical Isolate of S. typhi. Science Journal of Microbiology, 2013, Article ID: sjmb-115.
[33]	Mbuthia, S.K., Wachira, F.N. and Koech, R.K. (2014) In Vitro Antimicrobial and Synergistic Properties of Water Soluble Green and Black Tea Extracts. African Journal of Microbiology Research, 8, 1527-1534. http://dx.doi.org/10.5897/AJMR2014.6655
[34]	Koech, K.R., Wachira, F.N., Ngure, R.M., Wanyoko, J.K., Bii, C.C., Karori, S.M. and Kerio, L.C. (2013) Antimicrobial, Synergistic and Antioxidant Activities of Tea Polyphenols. In: Méndez-Vilas, A., Ed., Microbial Pathogens and Strategies for Combating Them: Science, Technology and Education, Formatex Research Center, Badajoz, 971-981.
[35]	Li, H., Zhou, G.Y., Zhang, H.Y. and Liu, J.A. (2011) Research Progress on the Health Function of Tea Oil. Journal of Medicinal Plants Research, 5, 485-489.
[36]	Fazel, M., Sahari, M.A. and Barzegar, M. (2008) Determination of Main Tea Seed Oil Antioxidants and Their Effects on Common Kilka Oil. International Food Research Journal, 15, 209-217.
[37]	Pan, X., Niu, G. and Liu, H. (2003) Microwave-Assisted Extraction of Tea Polyphenols and Tea Caffeine from Green Tea Leaves. Chemical Engineering and Processing, 42, 129-133. http://dx.doi.org/10.1016/S0255-2701(02)00037-5
[38]	Zhang, L., Wang, Y., Wu, D., Xu, M. and Chen, J. (2011) Microwave-Assisted Extraction of Polyphenols from Camellia oleifera Fruit Hull. Molecules, 16, 4428-4437. http://dx.doi.org/10.3390/molecules16064428
[39]	Lee, C.P. and Yen, G.C. (2006) Antioxidant Activity and Bioactive Compounds of Tea Seed (Camellia oleifera Abel.) Oil. Journal of Agricultural and Food Chemistry, 54, 779-784. http://dx.doi.org/10.1021/jf052325a
[40]	Cherotich, L., Kamunya, S.M., Alakonya, A., Msomba, S.W., Uwimana, M.A., Wanyoko, J.K. and Owuor, P.O. (2013) Variation in Catechin Composition of Popularly Cultivated Tea Clones in East Africa (Kenya). American Journal of Plant Sciences, 4, 628-640. http://dx.doi.org/10.4236/ajps.2013.43081
[41]	Kerio, L.C., Wachira, F.N., Wanyoko, J.K. and Rotich, M.K. (2012) Characterization of Anthocyanins in Kenyan Teas: Extraction and Identification. Food Chemistry, 131, 31-38. http://dx.doi.org/10.1016/j.foodchem.2011.08.005
[42]	Kilel, E.C., Faraj, A.K., Wanyoko, J.K., Wachira, F.N. and Mwingirwa, V. (2013) Green Tea from Purple Leaf Coloured Tea Clones in Kenya—Their Quality Characteristics. Food Chemistry, 141, 769-775. http://dx.doi.org/10.1016/j.foodchem.2013.03.051
[43]	Ngure, F.M., Wanyoko, J.K., Mahungu, S.M. and Shitandi, A.A. (2009) Catechins Depletion Patterns in Relation to Theaflavin and Thearubigins Formation. Food Chemistry, 115, 8-14. http://dx.doi.org/10.1016/j.foodchem.2008.10.006
[44]	Peterson, J., Dwyer, J., Bhagwat, S., Haytowitz, D., Holden, J., Eldridge, A.L., Beecher, G. and Aladesanmi, J. (2005) Major Flavonoids in Dry Tea. Journal of Food Composition and Analysis, 18, 487-501. http://dx.doi.org/10.1016/j.jfca.2004.05.006
[45]	Obanda, M., Owuor, P.O. and Taylor, S.J. (1997) Flavanol Composition and Caffeine Content of Green Leaf as Quality Potential Indicators of Kenyan Black Teas. Journal of the Science of Food and Agriculture, 74, 209-215. http://dx.doi.org/10.1002/(SICI)1097-0010(199706)74:2<209::AID-JSFA789>3.0.CO;2-4
[46]	Wright, L.P., Mphangwe, N.I.K., Nyirenda, H.E. and Apostolides, Z. (2000) Analysis of Caffeine and Flavan-3-ol Composition in the Fresh Leaf of Camellia sinensis for Predicting the Quality of the Black Tea Produced in Central and Southern Africa. Journal of the Science of Food and Agriculture, 80, 1823-1830. http://dx.doi.org/10.1002/1097-0010(200010)80:13<1823::AID-JSFA702>3.0.CO;2-E
[47]	Fernández, P.L., Martín, M.J., González, A.G. and Pablos, F. (2000) HPLC Determination of Catechins and Caffeine in Tea. Differentiation of Green, Black and Instant Teas. Analyst, 125, 421-425. http://dx.doi.org/10.1039/a909219f
[48]	Arranz, S., Saura-Calixto, F., Shaha, S. and Kroon, P.A. (2009) High Contents of Nonextractable Polyphenols in Fruits Suggest that Polyphenol Contents of Plant Foods Have Been Underestimated. Journal of Agricultural and Food Chemistry, 57, 7298-7303. http://dx.doi.org/10.1021/jf9016652
[49]	Rajaei, M., Barzegar, M. and Sahari, M.A. (2008) Comparison of Antioxidative Effect of Tea and Sesame Seed Oils Extracted by Different Methods. Journal of Agricultural Science and Technology, 10, 345-350.
[50]	Shao, P., Liu, Q., Fang, Z. and Sun, P. (2014) Chemical Composition, Thermal Stability and Antioxidant Properties of Tea Seed Oils Obtained by Different Extraction Methods: Supercritical Fluid Extraction Yields the Best Oil Quality. European Journal of Lipid Science and Technology, 117, 355-365. http://dx.doi.org/10.1002/ejlt.201400259
[51]	Rajaei, A., Barzegar, M. and Yamini, Y. (2005) Supercritical Fluid Extraction of Tea Seed Oil and Its Comparison with Solvent Extraction. European Food Research and Technology, 220, 401-405. http://dx.doi.org/10.1007/s00217-004-1061-8
[52]	Saxena, D.K., Sharma, S.K. and Sambi, S.S. (2011) Comparative Extraction of Cottonseed Oil by n-Hexane and Ethanol. ARPN Journal of Engineering and Applied Sciences, 6, 84-89.
[53]	Owuor, P.O., Chavanji, A.M. and Munavu, R.M. (1985) Chemical Studies of the Kenyan Tea Seeds. I. Physical and Chemical Characteristics of the Kernel Oil. Tea, 6, 23-28.