Effect of Cyantraniliprole on Feeding Cessation of Q Biotype Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae)

DOI: 10.4236/ae.2015.32007   PDF   HTML   XML   4,414 Downloads   5,444 Views   Citations


The whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) is one of the most devastating agricultural pests in many cropping systems worldwide. Growers rely on the use of insecticides to control this pest. However, some insecticides do not reduce the feeding of B. tabaci fast enough to prevent the direct and indirect damage produced by this insect. The effect of a new insecticide, cyantraniliprole 10OD (CyazypyrTM), on the feeding of B. tabaci adults, was studied under laboratory conditions. Cyantraniliprole 10OD is an insecticide that belongs to the IRAC Group 28 with a new mode of action for sucking insects, which provides rapid feeding cessation by impairing muscle function, resulting in reduced transmission of important insect vectored crop diseases. Laboratory experiments were conducted to determine the effect of cyantraniliprole along with some other commercially available insecticides on the feeding of B. tabaci adults by measuring the excretion of honeydew as an indirect assessment of insect feeding. In these experiments, cyantraniliprole resulted in significantly higher reduction of honeydew excretion (64.0%) by Q biotype B. tabaci adults during the first 30 minutes of exposure than diafenthiuron, triazophos, acetamiprid and spiromesifen, with all treatments having no adult mortality. Observations between 1 and 48 hours after exposure indicated that cyantraniliprole had numerically higher or similar reduction in honeydew production as the other insecticides, but by 48 hours (mid and high rate) and 96 hours (high rate) of exposure, cyantraniliprole had significantly higher reduction of honeydew excretion than all other insecticides tested. Low adult mortality was observed during first 24 hours of exposure in all treatments. Cyantraniliprole resulted in numerical or significantly higher adult mortality than all other treatments at the later observation intervals (72 - 96 hours). The higher reduction in honeydew excretion by cyantraniliprole appeared to be related to faster feeding cessation during the initial hours of exposure by a combination of feeding cessation and direct mortality as the exposure time increased. These findings document significant effects of cyantraniliprole on feeding cessation in Bemisia tabaci.

Share and Cite:

Rattan, R. , Purohit, H. , Patel, C. , Suvagia, P. , Singh, V. , Portillo, H. , Annan, I. and Alvarez, J. (2015) Effect of Cyantraniliprole on Feeding Cessation of Q Biotype Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Advances in Entomology, 3, 56-64. doi: 10.4236/ae.2015.32007.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Servin-Villegas, R., Troyo-Dieguez, E. and Martinez-Carrillo, J.L. (2001) Wild Hosts of Bemisia argentifolii (Homoptera: Aleyrodidae) in Semiarid Northwest Mexico, Southwest. Entomology, 26, 239-244.
[2] Perring, T.M., Cooper, A.D., Rodriguez, R.J., Farrar, C.A. and Bellows Jr., T.S. (1993) Identification of a Whitefly Species by Genomic and Behavioural Studies. Science, 259, 74-77.
[3] Ellsworth, P.C. (1999) Whitefly Management in Arizona Cotton Status and Needs. In: Dugger, P. and Richter, D., Eds., Proceedings Beltwide Cotton Conferences, National Cotton Council, Memphis, 41-44.
[4] Ellsworth, P.C., Tronstad, R., Leser, J., Goodell, P.B., Godfrey, L.D., Henneberry, T.J., Hendrix, D., Brushwood, D. and Naranjo, S.E. (1999) In: Castle, S. and Nichols, R.L., Eds., Sticky Cotton Sources and Solutions, Univ. Arizona, Coop. Ext. Publ. No. AZ1156, IPM Series 13, 4 p.
[5] Oliveira, M.R.V., Henneberry, T.J. and Anderson, P. (2001) History, Current Status, and Collaborative Research Projects for Bemisia tabaci. Crop Protection, 20, 709-723.
[6] Dalton, R. (2006) Whitefly infestations: The Christmas Invasion. Nature, 443, 898-900.
[7] McKee, G.J., Goodhue, R.E., Zalom, F.G., Carter, C.A. and Chalfant, J.A. (2009) Population Dynamics and the Economics of Invasive Species Management: The Greenhouse Whitefly in California-Grown Strawberries. Journal of Environmental Management, 90, 561-570.
[8] Jones, D.R. (2003) Plant Viruses Transmitted by Whiteflies. European Journal of Plant Pathology, 109, 195-219. http://dx.doi.org/10.1023/A:1022846630513
[9] Naranjo, S.E., Ellsworth, P.C., Chu, C.C., Henneberry, T.J., Riley, D.G., Watson, T.F. and Nichols, R.L. (1998) Action Thresholds for the Management of Bemisia tabaci (Homoptera: Aleyrodidae) in Cotton. Journal of Economic Entomology, 91, 1415-1426. http://dx.doi.org/10.1093/jee/91.6.1415
[10] Hector, D.J. and Hodkinson, I.D. (1989) Stickiness in Cotton. CAB International, Oxon, 43.
[11] Anderson, P.K., Hamon, A.B., Hernández, M.P. and Martin, J. (2005) Whiteflies as Vectors of Viruses in Legume and Vegetable Mixed Cropping Systems in the Tropical Lowlands of Central America, Mexico and the Caribbean. Reproductive Crop Hosts of Bemisia tabaci (Gennadius) in Latin America and the Caribbean. In: Anderson, P.K. and Morales, F.J., Eds., Whitefly and Whitefly-Borne Viruses in the Tropics: Building a Knowledge Base for Global Action, Centro Internacional de Agricultura Tropical (CIAT), Cali, 243-250.
[12] Elbert, A. and Nauen, R. (2000) Resistance of Bemisia tabaci (Homoptera: Aleyrodidae) to Insecticides in Southern Spain with Special Reference to Neonicotinoids. Pest Management Science, 56, 60-64.
[13] Ahmad, M., Arif, M.I., Ahmad, Z. and Denholm, I. (2002) Cotton Whitefly (Bemisia tabaci) Resistance to Organophosphate and Pyrethroid Insecticides in Pakistan. Pest Management Science, 58, 203-208.
[14] Nauen, R., Stumpf, N. and Elbert, A. (2002) Toxicological and Mechanistic Studies on Neonicotinoid Cross Resistance in Q-Type Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Management Science, 58, 868-875. http://dx.doi.org/10.1002/ps.557
[15] Roditakis, E., Roditakis, N.E. and Tsagkarakou, A. (2005) Insecticide Resistance in Bemisia tabaci (Homoptera: Aleyrodidae) Populations from Crete. Pest Management Science, 61, 577-582.
[16] Erdogan, C., Moores, G.D., Gurkan, M.O., Gorman, K.J. and Denholm, I. (2008) Insecticide Resistance and Biotype Status of Populations of the Tobacco Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) from Turkey. Crop Protection, 27, 600-605. http://dx.doi.org/10.1016/j.cropro.2007.09.002
[17] Roditakis, E., Grispou, M., Morou, E., Kristoffersen, J.B., Roditakis, N., Nauen, R., Vontas, J. and Tsagkarakou, A. (2009) Current Status of Insecticide Resistance in Q Biotype Bemisia tabaci Populations from Crete. Pest Management Science, 65, 313-322. http://dx.doi.org/10.1002/ps.1690
[18] Luo, C., Jones, C.M., Devine, G., Zhang, F., Denholm, I. and Gorman, K. (2010) Insecticide Resistance in Bemisia tabaci Biotype Q (Hemiptera: Aleyrodidae) from China. Crop Protection, 29, 429-434.
[19] Yuan, L., Wang, S., Zhou, J., Du, Y., Zhang, Y. and Wang, J. (2012) Status of Insecticide Resistance and Associated Mutations in Q-Biotype of Whitefly, Bemisia tabaci from Eastern China. Crop Protection, 31, 67-71. http://dx.doi.org/10.1016/j.cropro.2011.09.017
[20] IRAC International Working Group (2014) IRAC MoA Classification Scheme, Version 7.3.
[21] Castle, S., Palumbo, J. and Prabhaker, N. (2009) Newer Insecticides for Plant Virus Disease Management. Virus Research, 141, 131-139. http://dx.doi.org/10.1016/j.virusres.2008.12.006
[22] Stansly, P.A., Kostyk, B. and Riefer, R. (2010) Effect of Rate and Application Method of Cyazypyr (HGW86) on Control of Silverleaf Whitefly and Southern Armyworm in Staked Tomato, 2009. Arthropod Management Tests, 35, E43.
[23] Jacobson, A.L. and Kennedy, G.G. (2011) The Effect of Three Rates of Cyantraniliprole on the Transmission of Tomato Spotted Wilt Virus by Frankliniella occidentalis and Frankliniella fusca (Thysanoptera: Thripidae) to Capsicum annuum. Crop Protection, 30, 512-515.
[24] Jacobson, A.L. and Kennedy, G.G. (2013) Effect of Cyantraniliprole on Feeding Behavior and Virus Transmission of Frankliniella fusca and Frankliniella occidentalis (Thysanoptera: Thripidae) on Capsicum annuum. Crop Protection, 54, 251-258. http://dx.doi.org/10.1016/j.cropro.2013.08.018
[25] Jacobson, A.L. and Kennedy, G.G. (2014) Electrical Penetration Graph Studies to Investigate the Effects of Cyantraniliprole on Feeding Behavior of Myzus persicae (Hemiptera: Aphididae) on Capsicum annuum. Pest Management Science, 70, 836-840. http://dx.doi.org/10.1002/ps.3626
[26] Govindappa, M.R., Bhemanna, M., Arunkumar, H. and Ghante, V.N. (2013) Bio-Efficacy of Newer Insecticides against Tomato Leaf Curl Virus Disease and Its Vector Whitefly (Bemisia tabaci) in Tomato. International Journal of Applied Biology and Pharmaceutical Technology, 4, 226-231.
[27] Melamed-Madjar, V., Gerson, U. and Tal, S. (1983) A Laboratory Method for Estimating Survival of Tobacco Whitefly Nymphs after Insecticide Treatment, Based on Honeydew Excretion. Phytoparasitica, 11, 25-32. http://dx.doi.org/10.1007/BF02980708
[28] Costa, H.S., Toscano, N.C., Hendrix, D.L. and Henneberry, T.J. (1999) Patterns of Honeydew Droplet Production by Nymphal Stages of Bemisia argentifolii (Homoptera: Aleyrodidae) and Relative Composition of Honeydew Sugars. Journal of Entomological Science, 34, 305-313.
[29] Hendrix, D.L., Wei, Y. and Leggett, J.E. (1992) Homopteran Honeydew Sugar Composition Is Determined by Both Insect and Plant Species. Comparative Biochemistry and Physiology Part B, 101, 23-27.
[30] Llewellyn, M. (1971) The Effects of the Lime Aphid, Eucallipterus tiliae L. (Aphididae) on the Growth of the Lime Tilia × Vulgaris Hayne. I. Energy Requirements of the Aphid Population. Journal of Applied Ecology, 9, 261-282. http://dx.doi.org/10.2307/2402061
[31] Nauen, R., Koob, B. and Elbert, A. (1998) Antifeedant Effects of Sublethal Dosages of Imidacloprid Bemisia tabaci. Entomologia Experimentalis et Applicata, 88, 287-293.
[32] He, Y., Zhao, J., Wu, D., Wyckhuys, K.A.G. and Wu, K. (2011) Sublethal Effects of Imidacloprid on Bemisia tabaci (Hemiptera: Aleyrodidae) under Laboratory Conditions. Journal of Economic Entomology, 104, 833-838. http://dx.doi.org/10.1603/EC10281
[33] Davidson, E.W., Patron, R.B.R., Lacey, L.A., Frutos, R., Vey, A. and Hendrix, D.L. (1996) Activity of Natural Toxins against the Silverleaf Whitefly, Bemisia argentifolii, Using a Novel Feeding Bioassay System. Entomologia Experimentalis et Applicata, 79, 25-32.
[34] Cameron, R., Lang, E.B., Annan, I.B., Portillo, H.E. and Alvarez, J.M. (2013) Use of Fluorescence, a Novel Technique to Determine Reduction in Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) Nymph Feeding When Exposed to BeneviaTM and Other Insecticides. Journal of Economic Entomology, 106, 597-603.
[35] Cameron, R., Edward, B. and Alvarez, J.M. (2014) Use of Honeydew Production to Determine Reduction in Feeding by Bemisia tabaci (Hemiptera: Aleyrodidae) Adults When Exposed to Cyantraniliprole and Imidacloprid Treatments. Journal of Economic Entomology, 107, 546-550.
[36] Liu, T.X. (2004) Toxicity and Efficacy of Spiromesifen, a Tetronic Acid Insecticide, against Sweetpotato Whitefly (Homoptera: Aleyrodidae) on Melons and Collards. Crop Protection, 23, 505-513.
[37] Sethi, A. and Dilawari, V.K. (2008) Spectrum of Insecticide Resistance in Whitefly from Upland Cotton in Indian Subcontinent. Journal of Entomology, 5, 138-147. http://dx.doi.org/10.3923/je.2008.138.147
[38] Shivanna, B.K., Naik, G.B., Nagaraja, R., Basavaraja, M.K., Kalleswara Swamy, C.M. and Karegowda, C. (2011) Bio Efficacy of New Insecticides against Sucking Insect Pests of Transgenic Cotton. International Journal of Science and Nature, 2, 79-83.
[39] Horowitz, R., Denholm, I. and Morin, S. (2007) Resistance to Insecticides in the TYLCV Vector, Bemisia tabaci. In: Czosnek, H., Ed., Tomato Yellow Leaf Curl Virus Disease: Management, Molecular Biology, Breeding for Resistance, Springer, Netherlands, 305-325.
[40] Civolani, S., Cassanelli, S., Chicca, M., Rison, J.L., Bassi, A., Alvarez, J.M., Annan, I.B., Parrella, G., Giorgini, M. and Fano, E.A. (2014) An EPG Study of the Probing Behavior of Adult Bemisia tabaci Biotype Q (Hemiptera: Aleyrodidae) Following Exposure to Cyantraniliprole. Journal of Economic Entomology, 107, 910-919. http://dx.doi.org/10.1603/EC13511
[41] Alvarez, J.M., Portillo, H.E. and Annan, I.B. (2011) The Effect of CyazypyrTM in Reducing Hemipteran Pest-Transmitted Diseases in Crop Plants. Proceedings of the Hemipteran-Plant Interactions Symposium, Piracicaba, 76. http://www.infobibos.com/Hemipteran/Proceedings%20Final.pdf
[42] Alvarez, J.M., Portillo, H.E., Annan, I.B. and Cameron, R.A. (2011) The Role of DuPont CyazypyrTM in Reducing Insect- Transmitted Plant Diseases. ESA 59th Annual Meeting, Reno.

comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.