Determination of P, Ca, Zn, Cd and Pb concentrations in muscle, gills, liver, gonads and skeletons of two natural populations of Atherina lagunae in North Tunis Lake, Tunisia

DOI: 10.4236/jwarp.2011.36052   PDF   HTML     4,640 Downloads   8,663 Views   Citations


In this study, zinc (Zn), cadmium (Cd), lead (Pb), phosphorus (P) and calcium (Ca) concentrations in mus-cles, gills, liver, gonads and skeletons of two natural populations of sand smelt Atherina lagunae (Teleostean, Atherinidae) normal and deformed, as well as bioaccumulation of these elements from the water and the sediment in the North Tunis Lake were investigated. The analysis of Ca was performed with flame atomic absorption spectrometry. The average concentrations of Ca in the different tissues analyzed show higher val-ues in healthy atherines except in gonads where the average concentration of Ca in deformed atherines sig-nificantly exceeds that in normal atherines (p < 0.05) and the spine Ca concentrations were similar in the two populations. Zinc, cadmium, lead and phosphorus were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). The Zn concentrations of Atherina lagunae in North Tunis Lake were very high compared to other studies in other lagoons. The highest concentrations were found in deformed atherines. The differences are significant for all tissues studied (p < 0.05). The average concentration of P in different tissues analyzed shows that the highest values were detected in the normal population of Atherina lagunae. The potential rate of Cd was below the detection limit in the different organs analyzed, in water and sediment.

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N. Ayed, E. Faure, J. Quignard and M. Trabelsi, "Determination of P, Ca, Zn, Cd and Pb concentrations in muscle, gills, liver, gonads and skeletons of two natural populations of Atherina lagunae in North Tunis Lake, Tunisia," Journal of Water Resource and Protection, Vol. 3 No. 6, 2011, pp. 421-428. doi: 10.4236/jwarp.2011.36052.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] [1] G. Pergnent and N. Ben Maiz, “Le lac de Tunis, unexem- ple de restauration d’une lagune méditerranéenne,” Actes de colloque, Brest, November 7th-8th, 2000, pp. 168-178.
[2] S. Turki, Z. Armi, E. Trabelsi and B. Maiz, “Nutrient Loading and Occurrence of Potentially Harmful Phyto- plankton Species in the North Lake of Tunis Tunisia,” Cahier de Biologie Marine, Vol. 49, 2008, pp. 311-321.
[3] N. Ayed, E. Faure, J. P. Quignard, F. Maamouri and M. Trabelsi, “Incidence of Kyphosis Deformities in Natural Population of Atherina lagunae (Trabelsi et al. 2002) from the Tunis North Lake, Tunisia,” Marine Biology, 2008, Vol. 153, No. 3, pp. 319-325. doi:10.1007/s00227-007-0813-y
[4] N. Ayed, R. M. Barthélémy, J. L. Da Prato, J. P. Quig- nard and M. Trabelsi, “Accumulation de Métaux (alu- minium et cuivre) Chez Atherina lagunae du lac nord de Tunis,” Revue Soc. Sci. Nat. De Tunisie, Vol. 3, 2009, pp. 1-9.
[5] L. Tomasik, W. Wawrzyniak and A. Wnnicki, “Oxygen Deficiency and Negative Temperature as Teratogenic Factors in Rainbow Trout Salmo gairdneri,” Acta Ichth. Piscat, Vol. 12, 1982, pp. 93-99.
[6] J. Mis, K. Bieniarz, P. Epler, M. Sokolowska-Miko- lajczyk and J. Chyb, “Incubation of Fertilized Common Carp (Cyprinus carpio) Eggs in Different Concentrations of Copper,” Pol. Arch. Hydrobiol, Vol. 42, 1995, pp. 269-276
[7] J. H. Beattie and D. Pascoe, “Cadmium Uptake by Rain- bow Trout, Salmo gairdneri Eggs and Alevins,” Journal of Fish Biology, Vol. 13, No. 5, 1978, pp. 631-637. doi:10.1111/j.1095-8649.1978.tb03477.x
[8] G. W. Holcombe, D. A. Benoit, E. N. Leonard and J. M. McKim, “Long-Term Affects of Lead Exposure on Three Generations of Brook Trout (Salvelinus fontinalis),” Journal of the Fisheries Research Board of Canada, Vol. 35, 1976, pp. 1084-1088.
[9] J. Mis and J. Bigaj, “Hatching Glands of Carp (Cyprinus Carpio) Embryos from the Eggs Incubated At Various Oncentrations of Zinc or Copper,” Pol. Arch. Hydrobiol, Vol. 44, 1997, pp. 153-155.
[10] J. Harte, C. Holdren, R. Schneider and C. Sfdrley, “Toxic A to Z: A Guide to Everyday Pollution Hazards,” Uni- versity of California Press, Berkeley, 1991.
[11] G. Nussey, J. H. Van Vuren and H. H. Du Preez, “Bio- accumulation of Chromium, Manganese, Nickel and Lead in the Tissues of the Moggel, Labeo Umbratus (Cyprini- dae), from Witbank Dam, Mpumalanga,” Water SA, Vol. 26 No. 2, 2000.
[12] R. N. Jackson, D. Baird and S. Els, “The Effect of the Heavy Metals Lead (Pb2+) and Zinc (Zn2+) on the Brood and Larval Development of the Burrowing Crustacean Callianassa kraussi,” Water SA, Vol. 31, 2005.
[13] G. B. Sangalang and M. J. O’Halloran “Adverse Effects of Cadmium on Brook Trout Testis and on In Vitro Tes- ticular Androgen Synthesis,” Biology of Reproduction, Vol. 9, 1973, pp. 394-403.
[14] A. Calabrese, F. P. Thurbermg, M. A. Dawsona and D. R. Wenzloff, “Sublethal Physiological Stress Induced by Cadmium and Mercury in the Winter Flounder,” Pseudo- pleuronectes. cmmericanus, 1975, pp. 15-21.
[15] J. Koyama and Y. Itazawa, “Effect of Oral Administra- tion of Cadmium on Fish I. Analyticl Results of The Blood and Bones,” Bulletin of the Japanese Society of Scientific Fisheries, Vol. 43, 1977, pp. 523-526.
[16] B. E. Bengtsson, “Vertebral Damage in Wsh Induced by Pollutants,” In: J. E. Brown (Ed.) Sublethal Effects of Toxic Chemicals on Aquatic Animals, Elsevier, Amster- dam, 1975, pp. 22-30.
[17] T. R. Henry, J. M.Spitsbergen, M. W. Hornung, C. C. Abnet and R. E. Peterson, “Early Life Stage Toxicity of 2,3,7,8-Tetrachlorodibenzo-P-Dioxin in Zebrafish (Danio Rerio) ,” Toxicol Appl Pharmacol, Vol. 142, No. 1, 1997, pp. 56-68.
[18] P. M. Mehrle, T. A Haines, S. Hamilton, J. L. Ludke, T. L. Maye and M. A. Ribick, “Relation between Body Contaminants and Bone Development in East-Coast Striped Bass,” Transactions of the American Fisheries Society, Vol. 111, 1982, pp, 231-241.
[19] P. E. Olsson, L. Westerlund, S. J. Teh, K. Billsson, A. H. Berg, M. Tysklind, J. Nilsson, L. O. Eriksson and D. E. Hinton, “Effects of Maternal Exposure to Estrogen and PCB on Different Life Stages of Zebrafish (Danio Re- rio),” Royal Swedish Academy of Sciences, 1999, Vol. 28, pp. 100-106.
[20] H. Teraoka, W. Dong, S. Ogawa, S. Tsukiyama, Y. Oku- hara, M. Niiyama, N. Ueno, R. Peterson and T. Hiraga, “2,3,7,8-Tetrachlorodibenzo-P-Dioxin Toxicity in the Zebrafish Embryo: Altered Regional Blood Flow and Impaired Lower Jaw Development,” Toxicological Sci- ences, Vol. 65, 2002, pp. 192-199. doi:10.1093/toxsci/65.2.192
[21] S. H. Cheng, A. W. K. Wai, C. H. So and R. S. S. Wu, “Cellular and Molecular Basis of Cadmium Induced De- formities in Zebrafish Embryos,” Environmental Toxi- cology and Chemistry, Vol. 19, No. 12, 2000, pp. 3024- 3031. doi:10.1002/etc.5620191223
[22] M. R. Fraser, T. A. Anderson and R. De Nys, “Ontoge- netic Development of the Spine and Spinal Deformities in Larval Barramundi (Lates Calcarifer) Culture,” Aquacult, Volume 242, No. 1-4, 2004, pp. 697-711.
[23] G. Gerhard, E. Kauffman, X. Wang, R. Stewart, J. Moore, C. Kasales, E. Demidenko and K. Cheng, “Life Spans and Senescent Phenotypes in Two Strains of Zebrafish (Danio Rerio),” Experimental Gerontology, Vol. 37, 2002, p. 1055. doi:10.1016/S0531-5565(02)00088-8
[24] S. -J. Kaushik, “Besoins et Apport en Phosphore Chez Les Poissons,” INRA Production Animales, Vol. 18, No. 3, 2005, pp. 203-208
[25] M. G. M. Alam, A. Tanaka, G. Allinson, L. J. B. Laurenson, F. Stagnitti and E. Snow, “A Comparison of Trace Element Concentrations on Cultured and Wild Carp (Cyprinus Carpio) of Lake Kasumigaura, Japan,” Ecotoxicology and Environmental Safety, Vol. 53, 2002, pp. 348-354. doi:10.1016/S0147-6513(02)00012-X
[26] N. Ayed, E. Faure, J.-P. Quignard, F. Maamouri and M. Trabelsi, “Données Preliminaries Surlesdé Formations du Squelettes Axial de Atherina lagunae (Trabelsi et al. 2002) Du Lac Nord De Tunis,” Congrès Franco-Cana- dien de Zoologie, 21-23 Septembre 2005, Université de Montréal, Québec, Canada.
[27] N. Ayed, E. Faure, J. P. Quignard and M. Trabelsi, “Etude De La Cyphose Chez Atherina Lagunae Du Lac Nord De Tunis,” Troisième Rencontres Icthyologique de France, 28-31 Mars 2006, Paris, France.
[28] J. Dulcic, “Incidence of Spinal Deformities in Natural Populations of Grass Goby, Zosterisessor Ophiocephalus from the Karin Sea, Eastern Middle Adriatic,” Cybium 28, 2004, pp. 7-11.
[29] P. Tutman, B. Glamuzina, B. Skaramuka, V. Koqul, N. Glavi and D. Dului, “Incidence of Spinal Deformities in Natural Populations of Sandsmelt, Atherina boyeri (Risso, 1810) in the Nerevta River Estuary, Middle Adreatic,” Fish Research, Vol. 45, 2000, pp. 61-64. doi:10.1016/S0165-7836(99)00098-3
[30] J. M. Afonso, D. Monter, L. Robaina, N. Astorga, M. S. Izquierdo and R. Gines, “Association of a Lordosis- Scoliosis-Kyphosis Deformity in Gilthead Sea Bream (Sparus aurata) with Family Structure,” Fish Physiology and Biochemistry, Vol. 24, 2000, pp. 159-163. doi:10.1023/A:1007811702624
[31] M. Antunes and L. Da P. Cunha, “Skeletal Anomalies in Gobius Niger (Gobiidae) from Sado Estuary, Portugal,” Cybium, Vol. 26, 2002, pp.1791-1784.
[32] J. S. Weiss and P. Weiss, “Abnormal locomotion associ- ated with skeletal malformations in the sheep head min- now, Cyprinidon variegatus, exposed to Malathion, En- vironmental Research, 12, 1976, pp. 196-200
[33] R. I. Kroger and J. F. Guthrie, “Incidence of Crooked Vertebral Columns in Juvenile Atlantic Menhaden, Brevoortia tyrannus,” Chesapeake Science, Vol. 2, 1971, pp. 276-278.
[34] S. P. Lall, L. M. Lewis-McCrea, “Role of Nutrients in Skeletal Metabolism and Pathology in Fish – An Over- view,” Aquaculture, Vol. 267, 2007, pp. 3-19. doi:10.2307/1350917
[35] P. Divanach, C. Boglione, B. Menu, G. Koumoundouros, M. Kentouri and S. Cataudella, “Abnormalities in finfish mariculture: An overview of the problem, causes and so- lutions,” In: M. C. Saroglia, J. Sweetman and P. Lavens, Ed., Seabass and Seabream Culture: Problems and Prospects, Oostende, 1996, pp. 45-66. doi:10.1016/j.aquaculture.2007.02.053
[36] L. Madsen, J. Arnbjerg and I. Dalsgaard, “Radiological Examination of the Spinal Column in Farmed Rainbow Trout Oncorhynchus mykiss (Walbaum): Experiments with Flavobacterium psychrophilum and Oxytetracycline,” Aquaculture Research, Vol. 32, 2001, pp. 235- 241.
[37] L. M. Lewis, S. P. Lall and P. E. Witten, “morphological Descriptions Of The Early Stages Of Spine and Vertebral Development in Hatchery-Reared Larval and Juvenile Atlantic Halibut (Hippoglossus Hippoglossus),” Aqua- culture, Vol. 241, No. 1-4, 2004, pp. 47-59. doi:10.1046/j.1365-2109.2001.00552.x
[38] E. Favaloro and A. Mazzola, “Meristic Character Analysis and Skeletal Anomalies during Growth in Reared Sharpsnout Seabream,” Aquaculture, Vol. 8, 2000, pp. 417-430. doi:10.1016/j.aquaculture.2004.08.018
[39] H. Karadede, S. A. Oymak and E. U¨nlu¨, “Heavy Metals in Mullet, Liza abu, and Catfish, Siluris triostegus, from the Atat¨urk Dam Lake (Euphrates), Turkey,” Environment International, Vol. 30, 2004, pp. 183-188. doi:10.1023/A:1009284421354
[40] M. Canli and G. Atli, “The Relationships between Heavy Metal (Cd, Cr, Cu, Fe, Pb, Zn) Levels and Size of Six Mediterranean Fish Species,” Environmental Pollution, Vol. 121, 2003, pp. 129-136. doi:10.1016/S0160-4120(03)00169-7
[41] O. D. Uluozlu, M. Tuzen, D. Mendil and M. Soylak, “Trace Metal Content in Nine Species of Fish from the Black and Aegean Seas, Turkey.” Food Chemistry, Vol. 104, No. 2, 2007, pp. 835-840. doi:10.1016/S0269-7491(02)00194-X
[42] K. Uysal, Y. Emre and E. K?se ,“The Determination of Heavy Metal Accumulation Ratios in Muscle, Skin and Gills of Some Migratory Fish Species by Inductively Coupled Plasma-Optical Emission Spectrometry (ICP- OES) in Beymelek Lagoon (Antalya/Turkey),” Micro- chemical Journal, Vol. 90, No. 1, 2008, pp. 67-70. doi:10.1016/j.foodchem.2007.01.003
[43] M. Türkmen and C. Ciminli, “Determination of Metals in Fish and Mussel Species by Inductively Coupled Plasma- Atomic Emission Spectrometry,” Food Chemistry, Vol. 103, 2007, pp. 670-675. doi:10.1016/j.microc.2008.03.005
[44] C. Boglione, C. Costa, M. Giganti, M. Cecchetti, P. Di- dato and M. Scardi, “Biological Monitoring of Wild Thicklip Grey Mullet (Chelon Labrosus), Goldengrey Mullet (Liza Aurata), Thinlip Mullet (Liza Ramada) and Flathead Mullet (Mugil Cephalus) (Pisces: Mugilidae) from Different Adriatic Sites: Meristic Counts and Skeletal Anomalies,” Ecological Indicators, Vol. 6, 2006, pp. 712-732.
[45] J. Burger, “Assessment and Management of Risk to Wildlife from Cadmium,” Science of the Total Environ- ment, Vol. 389, 2008, pp. 37-45. doi:10.1016/j.ecolind.2005.08.032
[46] M. Romeo and M. Gnassia-Barelli, “Metal Distribution in Different Tissues and in Subcellular Fractions of the Mediterranean Clam Ruditapes Decussatus Treated With Cadmium, Copper, Or Zinc,” Comparative Biochemistry and Physiology, Vol. 111, 1995, pp. 457-463

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