Evaluating the p-FOXO1/FOXO1 Ratio: An Alternative Strategy for Endometrial Cancer Diagnosis


Background: The FOXO subfamily of Forkhead transcription factors plays a central role in pro-moting expression of proapoptotic and cell cycle regulatory genes. FOXO1 expression has an im-portant role in human endometrium homeostasis. Therefore, reduced FOXO1 protein and its inactivation by phosphorylation might, play a role in progression of human endometrial cancer. Methods: Current study was designed to investigate the changes of the FOXO1, phosphorylated-FOXO1 (p-FOX1) proteins levels and the p-FOXO1/FOXO1 ratio in 30 patients with endometrial cancer and 20 subjects with normal endometrium, surgically using excised human endometrial tissue specimens, quantitative real time PCR and western blot methods. Results: FOXO1 protein level in patients with endometrial cancer significantly reduced in comparison with control group (0.17 ± 0.15 vs. 1 ± 0.14; p < 0.001). Difference between p-FOXO1 level in patients with endometrial cancer and the control group was not significant (0.77 ± 0.65 vs. 1 ± 0.19; p = 0.13). It was noteworthy that P-FOXO1/FOXO1 ratio in patients with endometrial cancer was elevated (4.43 ± 0.38 vs. 1 ± 0.18; p < 0.01). Conclusion: Findings of this study indicate the importance of FOXO1 activity in endometrial cancer cell biology and suggest that enhancing FOXO1 function may be a compelling strategy to combat endometrial cancer progression.

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Korani, M. and Fallah, S. (2015) Evaluating the p-FOXO1/FOXO1 Ratio: An Alternative Strategy for Endometrial Cancer Diagnosis. International Journal of Clinical Medicine, 6, 217-226. doi: 10.4236/ijcm.2015.63027.

Conflicts of Interest

The authors declare no conflicts of interest.


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