Phylogenetic Analysis and Taste Cell  Expression of Calpain 9 in Catfish  (Ictalurus punctatus)

Tetsuya Ookura; Eiki Koyama; Anne Hansen; John H. Teeter; Yukio Kawamura; Joseph G. Brand

doi:10.4236/ns.2015.73016

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Natural Science > Vol.7 No.3, March 2015

Phylogenetic Analysis and Taste Cell Expression of Calpain 9 in Catfish (Ictalurus punctatus) ()

Tetsuya Ookura^1,2, Eiki Koyama^3,4, Anne Hansen⁵, John H. Teeter¹, Yukio Kawamura^2,6, Joseph G. Brand^1,3,7

¹Monell Chemical Senses Center, Philadelphia, USA.
²Division of Food Function, National Food Research Institute, Tsukuba, Japan.
³Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, USA.
⁴Division of Orthopedic Surgery, The Children’s Hospital of Philadelphia, Philadelphia, USA.
⁵Cell and Developmental Biology, School of Medicine, University of Colorado, Denver, USA.
⁶Department of Food and Nutrition, Kyoto Women’s University, Kyoto, Japan.
⁷Veterans Affairs Medical Center, University of Pennsylvania, Philadelphia, USA.

DOI: 10.4236/ns.2015.73016 PDF HTML XML 2,920 Downloads 3,347 Views Citations

Abstract

The calpains, calcium-activated neutral proteases, play important roles in calcium-regulated intra-cellular signal transduction cascades. Here we report the isolation and initial characterization of a cDNA encoding a calpain 9, digestive tract specific calpain, from catfish taste epithelium. This calpain 9 (Ip-CAPN9a) shares 61% identity with human calpain 9. Phylogenetic analysis provides evidence that catfish calpain 9 and the related enzymes from Oncorhynchus mykiss, Danio rerio, Xenopus laevis, Mus musculus, Rattus norvegicus and Homo sapiens make up a distinct clade within the tissue-specific calpain family. Northern blot analysis reveals that Ip-CAPN9a is predominantly expressed in barbell and digestive tract, but not expressed in brain. An antibody against the N-terminal segment of Ip-CAPN9a recognizes cells within the taste buds in catfish barbells.

Keywords

Gene Evolution, Taste, Novel Type

Share and Cite:

Ookura, T. , Koyama, E. , Hansen, A. , Teeter, J. , Kawamura, Y. and Brand, J. (2015) Phylogenetic Analysis and Taste Cell Expression of Calpain 9 in Catfish (Ictalurus punctatus). Natural Science, 7, 143-150. doi: 10.4236/ns.2015.73016.

Conflicts of Interest

The authors declare no conflicts of interest.

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