Efficacy of Selected Plant Extracts against Pyricularia grisea, Causal Agent of Rice Blast Disease


Rice blast disease, caused by a seed-borne fungus Pyricularia grisea, is an important and serious disease of rice (Oryza sativa L.) worldwide. The disease has been reported to cause yield losses of up to 40% in Tanzania. Studies were conducted to determine the effect of aqueous extracts of Aloe vera, Allium sativum, Annona muricata, Azadirachta indica, Bidens pilosa, Camellia sinensis, Chrysanthemum coccineum, processed Coffee arabica, Datura stramonium, Nicotiana tabacum and Zingiber officinalis for control of rice blast disease (Pyricularia grisea) in-vitro and in-vivo. The results indicate that processed C. arabica at 10% and 25% (v/v) had the highest (81.12%) and (89.40%) inhibitory effect, respectively, against P. grisea. Aqueous extract from N. tabacum at 10% concentration ranked third (80.35%) in inhibiting P. grisea. These were followed by extracts from 25% A. vera (79.45%) and 25% C. coccineum flower (78.83%). The results also indicate that, extracts from A. indica, A. vera, A. sativum, C. arabica, D. stramonium, C. sinensis, Z. officinalis and N. tabacum did not have any phytotoxic effect on seed germination, shoot height, root length, dry weight, seedling growth and seedling vigour index. These plant extracts can thus be used for rice seed treatment to manage rice blast disease.

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Hubert, J. , Mabagala, R. and Mamiro, D. (2015) Efficacy of Selected Plant Extracts against Pyricularia grisea, Causal Agent of Rice Blast Disease. American Journal of Plant Sciences, 6, 602-611. doi: 10.4236/ajps.2015.65065.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Ghazanfar, M.U., Habib, A. and Sahi, S.T. (2009) Screening of Rice Germplasm against Pyricularia oryzae, the Cause of Rice Blast Disease. Pakistan Journal Phytopathology, 21, 41-44.
[2] Sukanya, S.L., Yamini, D. and Fathima, S.K. (2011) Eco-Friendly Management of Pyricularia oryzae, the Causal Agent of Blast of Paddy. Journal of Current Botany, 2, 46-49.
[3] TeBeest, D.O., Guerber, C. and Ditmore, M. (2007) Rice Blast. Journal of Plant Disease, 10,109-113.
[4] International Rice Research Institute (IRRI) (2010) Magnaporthegrisea: Rice Blast.
[5] Luzi-Kihupi, A., Zakayo, J.A., Tusekelege, H., Mkuya, M., Kibanda, N.J.M., Khatib, K.J. and Maerere, A. (2009) Mutation Breeding for Rice Improvement in Tanzania. Food and Agriculture Organization, United Nations, Rome.
[6] Kato, K. (2007) Development of a Major Rice Cultivation Area in the Kilombero Valley, Tanzania. African Study Monographs, 36, 3-18.
[7] Mossini, S.A.G., Oliveira, K.P. and Kemmelmeier, C. (2004) Inhibition of Patulin Production by Penicilliumexpansum Cultured with Neem (Azadirachtaindica) Leaf Extracts. Journal of Basic Microbiology, 44, 106-113.
[8] Mwilene, F.E., Oikeh, S.O., Agunibiade, T.A., Oladimeji, O., Ajayi, O., Sie, M., Gregorio, G.B., Togola, A. and Toure, A.D. (2008) Growing Lowland Rice. In: Production Handbook, Africa Rice Center (WARDA), Bouaké.
[9] International Rice Research Notes (IRRN) (1993) Treating Rice Seeds with Fungicides and Antagonists to Control Seed-Borne Diseases.
[10] Mbodi, Y., Gaye, S. and Diaw, S. (1986) The Role of Tricyclazole in Rice Protection against Blast and Cultivar Improvement. Journal of Parasitica, 43, 187-198.
[11] Khan, Z.S. and Nasreen, S. (2010) Phytochemical Analysis, Antifungal Activity and Mode of Action of Methanol Extracts from Plants against Pathogens. Journal of Agricultural Technology, 6, 793-805.
[12] Peter, A.C.O. (2005) Non-Pesticide Methods for Controlling Diseases and Insect Pests. Integrated Pest Management Expert and Food and Agriculture Organization, Bangkok.
[13] Amadioha, A.C. (2000) Controlling Rice Blast in Vitro and in Vivo with Extracts of Azadirachta indica. Journal of Crop Protection, 19, 287-290.
[14] Jabeen, R. (2006) Evaluation of Botanicals against Xanthomonas oryzae pv. oryzae Using Bacterial Leaf Blight Disease of Rice and Characterization of Bioactive Compounds. MSc. Dissertation, Quaid-i-Azam University, Islamabad.
[15] Lalitha, V., Raveesha, K.A. and Kiran, B. (2010) Antimicrobial Activity of Solanum torvum Swart. against Important Seed Borne Pathogens of Paddy. Iranica Journal of Energy & Environment, 1, 160-164.
[16] Enikuomehin, O.A. (2005) Cercospora Leaf Spot Disease Management in Sesame (Sesamum indicum L.) with Plant Extracts. Journal of Tropical Agriculture, 43, 19-23.
[17] Gurjar, M.S., Ali, S., Akhtar, M. and Singh, K.S. (2012) Efficacy of Plant Extracts in Plant Disease Management. Journal of Agricultural Sciences, 3, 425-433.
[18] Mathur, S.B. and Kongsdal, O. (2004) Common Laboratory Seed Healthy Testing Methods for Detecting Fungi. 2nd Edition, International Seed Testing Association (ISTA), Bassersdorf.
[19] Harlapur, S.I., Kulkarni, M.S., Wali, M.C. and Kulkarni, S. (2007) Evaluation of Plant Extracts, Bio-Agents and Fungicides against Exserohilum turcicum (Pass.) Leonard and Suggs. Causing Turcicum Leaf Blight of Maize. Journal of Agricultural Sciences, 20, 541-544.
[20] Akinbode, O.A. and Ikotun, T. (2008) Evaluation of Some Bio-Agents and Botanicals in in Vitro Control of Colletotrichuwm destructivum. African Journal of Biotechnology, 7, 868-872.
[21] Hasan, M.M., Chowdhury, S.P., Alam, S., Hossain B. and Alam, M.S. (2005) Antifungal Effects of Plant Extracts on Seed-Borne Fungi of Wheat Seed Regarding Seed Germination, Seedling Healthy and Vigor Index. Journal of Biological Sciences, 8, 1284-1289.
[22] Nduagu, C., Ekefan, E.J. and Nwankiti, A.O. (2008) Effect of Some Crude Plant Extracts on Growth of Colletotrichum capsici (Synd) Butler and Bisby, Causal Agent of Pepper Anthracnose. Journal of Applied Biosciences, 6, 184-190.
[23] Zida, E.P., Sereme, P., Leth, V. and Sankara, P. (2008) Effect of Acacia gourmaensis A. Chev and Eclipta alba (L.) Hassk. on Seed Health, Seedling Vigour and Grain Yield of Sorghum and Pearl Millet. Asian Journal of Plant Pathology, 2, 40-47.
[24] Mamiro, D.P. and Royse, D.J. (2004) Laboratory Efficacy of Selected Fungicides and Rhododendron catawbiense Leaf Extracts on the Growth of Vericillium fungicola. Acta Edulis Fungi, 12, 390-396.
[25] Kamalakannan, A. and Shanmugam, V. (2005) Controlling Downy Mildew of Maize Caused by Peronosclerospora sorghi by Prosopis chilensis, Azadirachta indica and Foliar Sprays of a Talc-Based Formulation of a Pseudomonas fluorescens. Plant Pathology, 18, 115-118.
[26] Ogbebor, N. and Adekunle, A.T. (2005) Inhibition of Conidial Germination and Mycelial Growth of Corynespora cassiicola (Berk and Curt) of Rubber (Hevea Brasiliensis Muell. Arg.) Using Extracts of Some Plants. African Journal of Biotechnology, 4, 996-1000.
[27] Namai, T. and Ehara, Y. (1986) Studies on Variation in Virulence of Rice Blast Fungus, Pyricularia oryzae Cavara. Journal of Agricultural Research, 36, 3-4.
[28] Chala, A., Brurberg, M.B. and Tronsmo, A.M. (2010) Incidence and Severity of Sorghum Anthracnose in Ethiopia. Plant Pathology, 9, 23-30.
[29] International Seed Testing Association (ISTA) (2008) International Rules for Seed Testing. Bassersdorf.
[30] Mbega, E.R., Mortensen, C.N., Mabagala, R.B. and Wulff, E.G. (2012) The Effect of Plant Extracts as Seed Treatments to Control Bacterial Leaf Spot of Tomato in Tanzania. Journal of General Plant Pathology, 78, 277-286.
[31] Chhetri, S. (2009) Identification of Accelerated Aging Conditions for Seed Vigor Test in Rice (Oryza sativa L.). Ph.D. Thesis, Thawatchai Teekachunhatean, Nakhon Ratchasima.
[32] Kamalakannan, A., Shanmugam, V., Surendran, M. and Srinivasan, R. (2001) Antifungal Properties of Plant Extracts against Pyricularia grisea, the Rice Blast Pathogen. Indian Journal of Phytopathology, 54, 490-492.
[33] Taiga, A. and Friday, E. (2009) Variations in Phytochemical Properties of Selected Fungicidal Aqueous Extracts of Some Plant Leaves in Kogi State, Nigeria. American-Eurasian Journal of Sustainable Agriculture, 3, 407-409.
[34] Hajano, J.U., Lodhi, A.M., Pathan, M.A., Khanzada, M.A. and Shah, G.S. (2012) In Vitro Evaluation of Fungicides, Plant Extracts and Bio-Control Agents against Rice Blast Pathogen Magnaporthe oryzae Couch. Pakistan Journal of Botanical, 44, 1775-1778.
[35] Yeni, I.J. (2011) Evaluation of Antifungal Effects of Extracts of Allium sativum and Nicotiana tabacum against Soft Rot of Yam (Dioscorea alata). Journal of Agricultural Research, 3, 1-5.
[36] Obongoya, B.O., Wagai, S.O. and Odhiambo, G. (2009) Fungitoxic Properties of Four Crude Plant Extracts on Fusarium oxysporum Schl. F. sp. Phaseoli. African Journal of Food Agriculture Nutrition and Development, 9, 1652-1666.
[37] Nguefack, J., Torp, J., Leth, V., Dongmo, J.B., Fotio, D. and Amvam Zollo, P.H. (2007) Effects of Plant Extracts and Chemical Fungicide in Controlling a Rice Seed-Borne Fungus under Laboratory and in Irrigated Cropping System in Ndop-Cameroon. African Journal of Crop Science, 8, 791-796.

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