Pig Compost Use on Zinc and Copper Concentrations in Soils and Corn Plants ()
1. Introduction
Pig compost, known as “biostabilized” in Chile, is derived from the pork industry; Chile produced approximately 300,000 Mg in 2008. This product is characterized by its high Cu and Zn concentrations which do not comply with the limits indicated by the Chilean Compost Regulation [1] . Despite the fact that this organic amendment is an important nutritional source for agricultural crops, it has a comparatively lower cost than conventional fertilizers [2] [3] just like amendments derived from other types of meat production [4] - [6] . In contrast inorganic fertilizers, the organic amendments have high organic matter content and can be used as source of slow-release nitrogen. Therefore, agricultural production systems that use these amendments contribute to improving soil physical, chemical, and biological properties [4] [7] - [9] . However, the net supply rate of certain nutrients, such as nitrogen (N), is lower than the rate produced by conventional fertilizers [6] [10] [11] . In many cases, this situation has led farmers to increase the applied amendment rate to compensate N in the crop; this fact could cause certain environmental risks. The increment in the concentration of other elements generates potential health risk since they can enter the food chain or could have a negative effect on the plant development [12] - [14] . Heavy metals, such as Cu and Zn, are two of these elements; their high rates in the PC can affect crop yield through direct effects on the plant or indirect effects derived from soil nutrient antagonism [12] [15] - [17] . In this respect, several works about the effect of organic amendments on heavy metals in plant and soil had been reported in the world [12] [15] - [18] . Moreover, for the Chilean conditions, there is not information published.
Therefore, in order to contribute to a better knowledge of using PC and its effects on a very important crop for world food supply, as well as for Chilean agriculture, the objective of this study was to evaluate the use of increasing PC rates on corn production, Zn and Cu concentrations in plant tissues, and the extractable concentration of these elements in the soil.
2. Materials and Methods
2.1. Experimental Sites
Two experiments were conducted in south central Chile with the Divino-SEMAMERIS corn hybrid during the 2010-2011 season to evaluate the effect of increasing PC rates and the associated contribution of Cu and Zn. The locations were Chillán (36˚36'S, 71˚54'W) with a silty loam soil (Melanoxerands) [19] [20] and Talca (35˚23.279'S, 71˚36.57'W) with a clay loam soil (Ultic Haploxerolls) [19] [21] . The crop in both locations was fertilized with the following treatments: 1) Control without fertilization (C); 2) Conventional fertilization (CF) N, phosphorus (P), and potassium (K) with known sources (urea, triple superphosphate, muriate of potash) at rates equal to PC contribution for an equivalent rate of 350 kg N ha−1; 3) PC at N rates equivalent to 350 kg・ha−1 (15.33 Mg・ha−1) (N:P2O5:K2O ratio of 1:1.44:0.49); 4) PC at N rates equivalent to 700 kg・ha−1 (30.65 Mg・ha−1); 5) PC at N rates equivalent to 1400 kg・ha−1 (61.31 Mg・ha−1). The experimental design was split plot with four replicates in both locations and where the main plot corresponded to the location and the subplot to the fertilization treatment. Each plot within a block was 5 m long × 5 m wide with five rows of corn drilled at 0.70 m row spacing. The chemical characteristics of PC used in both experiments are shown in Table 1, whereas the chemical characteristics of both soils are shown in Table 2.
The climate in both locations is Mediterranean with high temperatures and low rainfall during summer and lower temperatures and high rainfall during winter. The accumulated rain fall in Chillán was 776 mm, which was concentrated between May and October along with an annual evaporation of 1275 mm and average temperature of 13.0˚C. On the other hand, accumulated rainfall in Talca was 750 mm, which was concentrated between May and September along with an annual evaporation of 1360 mm and average temperature of 14.6˚C. The trial site in both locations had been cropped with corn in previous years.
2.2. Crop Husbandry
All plots were cultivated to optimize crop growth according to standard agronomic practices for corn in central Chile. The trial site was plowed each year during the winter. The soil was prepared with conventional tillage equipment to establish an acceptable seedbed. Seeds were drilled with a disinfected standard drill. After emergence, weeds were controlled with a combination of herbicides depending on the observed weed pressure. The initial plant population for Chillán and Talca was 108,600 and 112,200 plants・ha−1, respectively.
The crop in Chillán was sowing on 21 October 2010 and grain harvested on 15 April 2011. The dates for these activities in Talca were 15 October 2010 and 2 April 2011, respectively. Whole plant moisture was approximately 15% at grain maturity. Pig compost was previously applied at sowing and for CF; P and K were applied at sowing and N was applied 40% at sowing and 60% at the 6-leaf stage. For whole plant or grain harvest
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Table 1. Physico-chemical characteristics of pig compost (PC) applied in both experiments.
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Table 2. Chemical characteristics of the soils used in the study.
EC: electric conductivity, OM: organic matter.
in each plot, 20 and 10 contiguous plants from the central rows were collected; the whole plant was cut at 10 cm above the soil surface and weighed. Harvested weights were recorded as fresh yields and the moisture content was determined by oven drying at 70˚C for 48 h. Grains were harvested by stripping the corn cob and drying at 70˚C for 96 h; grain yield was then adjusted to 14.5% moisture.
2.3. Data Collection and Analysis
Grain yield (GY), dry matter (DM) and Zn and Cu concentrations were determined. Dried plant subsamples were ground with a mill, passed through a 2 mm sieve, and analyzed. The Zn and Cu concentrations were determined by sample calcinations and atomic absorption spectrometry. Extractable soil Zn and Cu analysis was performed on 10 subsamples from each experimental unit collected at harvest at two depths of 0 - 20 and 20 - 40 cm. Samples were air dried, passed through a 2 mm sieve, and Zn and Cu analyzed by extraction with diethylen- etriamine-pentaacetic acid (DTPA) in a 1:2 ratio; the elements in solution were determined by flame atomic absorption spectrometry. In addition, pH was determined by directly measuring the 1:2.5 aqueous extract (Potentiometer) given its relationship with Zn and Cu availability [22] .
2.4. Statistical Analysis
Results were examined by ANOVA and Tukey’s multiple range test (p = 0.05) were used for the comparation of means with the SAS general model procedure [23] . When data were not normally distributed [24] , the diagnostic procedure was used to select the most appropriate transformation. Treatments and the locations were considered as fixed effects and the replicates as random effects.
3. Results
3.1. Chemical Soil Properties
The chemical properties in the two locations differed (Table 2) and only Talca exhibited an overall adequate fertility level for all the essential elements, except sulfur (S); this was pointed out by [25] . On the other hand, the initial levels of Zn and Cu found in Talca were not considered excessive for corn crop [22] [25] . High corn yields have also been reported in previous experiments for both locations [11] [26] . As soil pH affects Zn and Cu availability, the values obtained in the present study for this parameter in both locations might did not affect their availability (Table 2) [22] .
3.2. Whole Plant Dry Matter Production and Zinc and Copper Concentrations
Dry matter production was significantly different in the two locations (p < 0.01); it was also affected by the fertilization treatment (p < 0.01) and by the interaction between both sources of variation (p < 0.05) (Table 3). Whole plant Zn concentration was only affected by location (p < 0.01), whereas Cu concentration was affected by the type of fertilization (p < 0.01) and the location-fertilization treatment interaction (p < 0.01) (Table 3). High DM production in Chillán was obtained with CF (350 kg N ha−1) and PC2 (700 kg N ha−1) and PC3 (1400 kg N ha−1) (p < 0.05), which were higher than C (Figure 1).
Corn plant Zn concentration in response to fertilization treatments differed only in Talca (Figure 2); the highest concentration was obtained in the control, which surpassed CF and PC3 (1400 kg N ha−1) (p < 0.05), while PC1 and PC2 did not present significant differences compared to C. The Zn concentration fluctuated between 42.0 and 76.1 mg・kg−1 in Chillán and between 13.5 and 21.2 mg・kg−1 in Talca; these values were in the normal range mentioned by [27] for corn leaves.
Similar to corn plant Zn concentration, Cu concentration was different only in Talca (Figure 3), and the highest concentration was obtained in CF, and the lowest in PC3. The Cu content was significantly higher (p < 0.05) in CF than in any other treatments.
3.3. Grain Yield and Grain Zinc and Copper Concentrations
Grain yield was significantly different between locations (p < 0.05) and affected by the fertilization treatments (p < 0.01); there was no observed interaction between both factors (p > 0.05) (Table 3). Grain Zn concentration was not affected by any of the sources of variation (p > 0.05), whereas Cu concentration was affected by both
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Table 3. Significance of statistical analysis of parameters evaluated in the experiment.
**Significance < 0.01. *Significance between 0.01 and 0.05. nsNon significant. DM: dry matter.
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Figure 1. Total whole plant DM production at corn grain harvest in both locations. Different letters over the columns for the same location indicate a statistical difference among fertilization treatments according to Tukey’s test (p < 0.05). C: control without fertilization; CF: conventional fertilization at 350 kg N ha−1 rate; PC1: pig compost at 350 kg N ha−1 rate (15.33 Mg PC ha−1); PC2: pig compost at 700 kg N ha−1 rate (30.65 Mg PC ha−1); PC3: pig compost at 1400 kg N ha−1 rate (61.31 Mg PC ha−1).
location and type of fertilization (p < 0.01) and there was no location-fertilization treatment interaction (p > 0.05) (Table 3). The highest GY in Chillán was obtained with PC3 (1400 kg N ha−1), which was significantly different to C and PC1 (350 kg N ha−1) (p < 0.05) and did not presented differences among CF and PC2 (Figure 4). There was no difference in GY among the fertilized treatments in Talca (p > 0.05), whereas only CF surpassed C (p < 0.05) (Figure 4), this is likely attributable to the adequate soil chemical properties (Table 2).
Grain Zn concentration fluctuated between 23.7 and 42.2 mg・kg−1 in Chillán and between 26.2 and 33.8
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Figure 2. Whole plant Zn concentration at corn grain harvest in both locations. Different letters over the columns for the same location indicate a statistical difference among fertilization treatments according to Tukey’s test (p < 0.05). C: control without fertilization; CF: conventional fertilization at 350 kg N ha−1 rate; PC1: pig compost at 350 kg N ha−1 rate (15.33 Mg PC ha−1); PC2: pig compost at 700 kg N ha−1 rate (30.65 Mg PC ha−1); PC3: pig compost at 1400 kg N ha−1 rate (61.31 Mg PC ha−1).
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Figure 3. Whole plant Cu concentration at corn grain harvest in both locations. Different letters over the columns for the same location indicate a statistical difference among fertilization treatments according to Tukey’s test (p < 0.05). C: control without fertilization; CF: conventional fertilization at 350 kg N ha−1 rate; PC1: pig compost at 350 kg N ha−1 rate (15.33 Mg PC ha−1); PC2: pig compost at 700 kg N ha−1 rate (30.65 Mg PC ha−1); PC3: pig compost at 1400 kg N ha−1 rate (61.31 Mg PC ha−1).
mg・kg−1 in Talca (Figure 5). Although there were no significant differences in these concentrations (Table 3), an erratic effect was observed in quantitative terms in the evaluated fertilization treatments (Figure 5).
Corn grain Cu concentration fluctuated between 1.8 and 2.4 mg・kg−1 in Chillán and between 2.2 and 2.8 mg・kg−1 in Talca (Figure 6) and exhibited differences in fertilization treatments only in Talca where the highest content was obtained in PC3 treatment (1400 kg・ha−1). Only significantly difference was found between this treatment and the C while no differences were obtained among the fertilizer treatments.
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Figure 4. Corn grain yield in both locations. Different letters over the columns for the same location indicate a statistical difference among fertilization treatments according to Tukey’s test (p < 0.05). C: control without fertilization; CF: conventional fertilization at 350 kg N ha−1 rate; PC1: pig compost at 350 kg N ha−1 rate (15.33 Mg PC ha−1); PC2: pig compost at 700 kg N ha−1 rate (30.65 Mg PC ha−1); PC3: pig compost at 1400 kg N ha−1 rate (61.31 Mg PC ha−1).
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Figure 5. Total corn grain Zn concentration in both locations. Different letters over the columns for the same location indicate a statistical difference among fertilization treatments according to Tukey’s test (p < 0.05). C: control without fertilization; CF: conventional fertilization at 350 kg N ha−1 rate; PC1: pig compost at 350 kg N ha−1 rate (15.33 Mg PC ha−1); PC2: pig compost at 700 kg N ha−1 rate (30.65 Mg PC ha−1); PC3: pig compost at 1400 kg N ha−1 rate (61.31 Mg PC ha−1).
3.4. Effects on pH and Soil Zn and Cu Concentrations
Soil pH from 0 to 20 cm depth was significantly affected by location (p < 0.01), fertilization treatments (p < 0.01), and by the interaction between both variables (p < 0.05) (Table 3). Significant difference attributable to location was found only in the 20 to 40 cm soil stratum (p < 0.01) (Table 3). This soil chemical property was determined because changes in this parameter can affect soil Zn and Cu availability [22] . Chillán location exhibited a slight high pH mean in the second soil stratum; however, no significant differences were seen among treatments (Table 4). The highest pH in the first soil layer was found in PC3 treatment (1400 kg N ha−1), which was only significantly different with the CF treatment (Table 4). Soil pH in Talca was slight higher than Chillán for both soil depths (Table 3 and Table 4). Just as in Chillán, when comparing mean pH in both depths the highest value was found at 20 - 40 cm (Table 4). The differences detected in the first soil layer in Talca indicated that the highest pH was obtained in C, which present significantly differences with CF and PC3 (1400 kg N ha−1). On the other hand, similar results were obtained for Chillán, where the highest acidification was obtained with CF which was significantly different only with PC3 treatment.
Extractable soil Zn concentration in the surface soil layer (0 - 20 cm) showed only differences among fertilization treatments (p < 0.01) (Table 3); differences in the subsurface layer (20 - 40 cm) were found in both source of variation, location (p < 0.05) and fertilization treatments (p < 0.01) and there was no interaction between
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Figure 6. Total corn grain Cu concentration in both locations. Different letters over the columns for the same location indicate a statistical difference among fertilization treatments according to Tukey’s test (p < 0.05). C: control without fertilization; CF: conventional fertilization at 350 kg N ha−1 rate; PC1: pig compost at 350 kg N ha−1 rate (15.33 Mg PC ha−1); PC2: pig compost at 700 kg N ha−1 rate (30.65 Mg PC ha−1); PC3: pig compost at 1400 kg N ha−1 rate (61.31 Mg PC ha−1).
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Table 4. Soil pH at corn harvest in both locations.
Different letters in the same column indicate statistical difference according to Tukey’s test (p < 0.05); C: control without fertilization; CF: conventional fertilization at 350 kg N ha−1 rate; PC1: pig compost at 350 kg N ha−1 rate (15.33 Mg PC ha−1); PC2: pig compost at 700 kg N ha−1 rate (30.65 Mg PC ha−1); PC3: pig compost at 1400 kg N ha−1 rate (61.31 Mg PC ha−1); CV: coefficient of variation.
them (Table 3). Except for PC3 treatment the organic amendment applied did not produce any differences respect to the control in both soils (Table 5). For the first soil layer, PC application led to a slight, non-signifi- cant increase in extractable soil Zn contents in Talca location. Non-significantly differences were found among all treatments, this fact may be explained by the high coefficient of variation obtained (Table 5). The highest Zn concentration in the second soil stratum was observed with PC3 (1400 kg N ha−1), which surpassed only C and CF treatments (p < 0.01) and no differences between these former treatments was found (Table 5).
Extractable soil Cu concentration in the upper soil layer showed differences between locations (p < 0.001) and treatments (p < 0.01) and no interaction between these sources of variation was seen (Table 3). While for the second soil layer the extractable soil Cu was affected by both sources of variation (Table 3). Extractable soil Cu contents located in Chillán exhibited the same effect in both depths where the highest concentration was found at PC3 treatment (1400 kg N ha−1), which it value was significantly different to C and CF, while no differences were found among PC treatments (Table 6). As for soil Zn concentration, the high coefficient of variation for this nutrient in both stratums is emphasized (Table 6). There were no significant differences in extractable soil Cu contents location in Talca at any of the soil depths (Table 6).
4. Discussion
A positive DM production response was observed with the increasing PC rates (Figure 1), which indicates that
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Table 5. Soil Zn concentration at time to corn harvest in both locations.
Different letters in the same column indicate a statistical difference according to Tukey’s test (p < 0.05); C: control without fertilization; CF: conventional fertilization at 350 kg N ha−1 rate; PC1: pig compost at 350 kg N ha−1 rate (15.33 Mg PC ha−1); PC2: pig compost at 700 kg N ha−1 rate (30.65 Mg PC ha−1); PC3: pig compost at 1400 kg N ha−1 rate (61.31 Mg PC ha−1) CV: coefficient of variation.
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Table 6. Soil Cu concentration at time to corn harvest in both locations.
Different letters in the same column indicate a statistical difference according to Tukey’s test (p < 0.05); C: control without fertilization; CF: conventional fertilization at 350 kg N ha−1 rate; PC1: pig compost at 350 kg N ha−1 rate (15.33 Mg PC ha−1); PC2: pig compost at 700 kg N ha−1 rate (30.65 Mg PC ha−1); PC3: pig compost at 1400 kg N ha−1 rate (61.31 Mg PC ha−1); CV: coefficient of variation.
there was no negative effect of using PC in corn for the evaluated rates, associate to its slow N mineralization [2] [6] [10] . Dry matter production in the fertilized treatments fluctuated between 20.9 and 30.6 Mg・ha−1; these values were similar to those mentioned by [8] for silage corn in the same production area. No difference in DM production was observed in Talca (Figure 1) for the evaluated treatments (p > 0.05); this is attributable to soil chemical properties (Table 2). Dry matter production obtained in the fertilized treatments fluctuated between 31.8 and 35.0 Mg・ha−1, which was similar to values indicated by [26] .
Corn plant Zn concentration values obtained in Chillán were higher than those found by [28] for different corn plant tissues amended with sewage sludge compost; while the values obtained in Talca were lower than those found by those authors. The quantitative differences between both locations is likely due to the initial chemical properties of each soil (Table 2) since the availability of each nutrient is affected by the concentration of other nutrients, such as phosphorus and calcium, and the presence of secondary crystalline minerals found in higher proportions in Talca [22] . In general, an inversely proportional relationship was observed between corn plant Zn concentration and increasing PC rates in both locations (Figure 2). In contrast, [18] indicate significant increases in Zn concentration in ryegrass (Lolium multiflorum Lam.) stems and roots amended with sewage sludge at a rate of 140 Mg・ha−1 (730.5 mg・Zn・kg−1) as compared to C and CF with NPK contributions equivalent to using organic amendment.
For the corn plant Cu concentration in quantitative terms, an inversely proportional effect was observed for both locations with the increasing PC rates (Figure 3), associate to the reactions with the organic compounds applied [22] . Copper concentration fluctuated between 2.0 and 3.4 mg・kg−1 in Chillán and between 0.6 and 4.5 mg・kg−1 in Talca; these were below the normal range mentioned by [27] for corn leaves and similar to values pointed out by [28] for different corn plant tissues amended with sewage sludge compost. [18] experimented with ryegrass amended with sewage sludge at a rate of 140 Mg・ha−1 (204.5 mg・Zn・kg−1) and indicated that applying this organic amendment produced significant increases in Cu concentration in ryegrass stems and roots as compared to C and CF.
The same as in DM production, a positive response in GY was observed with increasing PC rates (Figure 4); there was no negative effect of using PC in corn crop for the evaluated rates, associate to slow availability of the total N applied and the chemicals reactions between organic compounds and the nutrients applied with the PC [29] . In Chillán GY in the treatments applied fluctuated between 14.4 and 17.7 Mg・ha−1, which is similar to values mentioned by [30] in central Chile and slightly lower than those found by [26] in south central Chile.
For Talca the GY in the fertilized treatments fluctuated between 18.2 and 20.1 Mg・ha−1; these values were similar to those indicated by [26] and higher than those found by [30] .
Grain Zn concentration values (Figure 5) were generally similar to those pointed out by [28] for corn amended with sewage sludge compost. Unlike the difference obtained in whole plant Zn concentration, there were no quantitative differences in grain concentration between locations; the mean value in the treatments with PC were 30.0 and 32.3 mg・kg−1 in Chillán and Talca, respectively.
The mean grain Cu concentration was higher in Talca than Chillán (Figure 6); this fact may be was attributable to the different chemical properties of both soils (Table 2). Grain Cu concentrations in both locations were similar to those mentioned by [28] for corn amended with sewage sludge compost.
Respect to the soil in both locations, the lower pH obtained with the conventional fertilization (urea) use, which is in agreement from this reported by [2] who indicated that the application of conventional fertilizer (urea) produced soil acidification. The acidification generated with the PC use in high rate for the first stratum in Talca is related to the use of composted organic amendments and its carbon mineralization and release of organic acids, as has also been reported by several authors [2] [11] [13] [29] [31] and too can be attributed to the nitrification of ammonia-N [22] [29] .
Although a difference in pH existed at the start of the experiment (Table 2 and Table 4), this difference increased in Talca. This is due to the higher level of clay content and its structural characteristics (crystalline clay type 1:1 and 2:1 soils in Talca compared to the amorphous clay soils in Chillán), which produced base accumulation by illuviation in the second soil stratum [22] .
Soil Zn concentration in Chillán showed the same effect in both soil depths and the highest concentration was obtained with PC3 (1400 kg N ha−1), which was only significantly different under C and CF treatments and no significant differences were seen among PC treatments (Table 5). The high variation coefficient found for this nutrient in both soil layers suggests, that long-term studies are needed in order to detect soil Zn concentration changes, such as the one carried out by [17] where organic amendments were applied to evaluate the over time, changes in soil heavy metal contents. The highest Zn concentration was found in the first stratum when comparing both soil layers, which was associated with the strong bonding of this nutrient to the organic matter, plus the higher pH in the subsurface soil layer [22] . Similar effects associated with applying increasing rates of sewage sludge compost were reported by [28] . Almost the same Chillán soil, the Zn concentration in Talca soil for first soil stratum are agree with the results obtained by [28] . The mean value of PC treatments for the first soil layer showed a higher Zn content in Talca soil than in Chillan soil, while the higher content in the second soil stratum was found in Chillán than in Talca soil (Table 5). The highest mean value of extractable soil Zn was found in Talca (Table 5), which agrees with the initial concentration of this nutrient in this soil (Table 2). The highest increase in Zn concentration in both soils was obtained in Chillán (Table 2 and Table 5); this is attributable to the type of clay, as well as lower soil pH [22] . The pH effect, which showed differences only between PC3 and C in the first soil layer could have contributed in increasing Zn concentration in this stratum [22] (Table 4 and Table 5). No toxicity reports exist for Zn in agricultural crops in Chile; this slight increase in concentration associated with PC at rates that meet crop nutritional needs does not constitute a risk as this alternative fertilization source.
The highest soil Cu concentration in Chillán was generally obtained in the first stratum (Table 6) associated with the low movement of this nutrient in the soil [22] . The results obtained agree with those registered by [28] in an increasing rates of sewage sludge compost study as well as differences in concentration between soil depths. As well as in Talca soil there were not differences between treatments for the soil Cu concentration, this lack of response may be due to the high coefficient of variation obtained in both soil strata (Table 6). Similarly to Chillán, a higher Cu concentration was found in the first soil stratum, but extractable soil Cu concentration in both stratums was higher in Talca (Table 6). However, considering the average applied treatments, the extractable soil Cu content regarding to the initial value, increased only in the Chillán soil (Table 2 and Table 6). This fact was attributable to the lower initial concentration and type of clay as well as lower soil pH [22] . The lack of differences among treatments and the slight increase in the extractable Cu concentration with regard to the initial value is an advantage for this organic amendment which has high Cu concentration. Soils high in crystalline clays, such as Talca soil are associated with the lack of response to Cu and Zn applied with the PC treatments. In addition, the soil pH in Talca did not affected the Cu availability, since only PC3 produced a lower pH than C in the first soil layer (Table 4), therefore the extractable soil Cu contents in this location and depths did not show significantly differences among treatments (Table 6). This effect is associated to chemicals reactions with the organic compounds presents in PC [29] .
5. Conclusion
The results obtained indicated that the composted pig manure applied did not negatively affect corn crop cultivated in two soils with different pedological characteristics. In both soils, applying PC at rates adjusted to the N nutritional needs for corn production, showed a slightly, non-significant decrease of GY production without effect on grain Zn and Cu contents with respect to the conventional fertilization. At the same time, the DM production and Zn concentration in plant were not affected with this PC rate with respect to the conventional fertilization, but the Cu concentration in plant was decreased significantly in one of the two locations. An increase with increasing application rate in extractable soil Zn and Cu contents was observed in both soils, where the greater impact was obtained on the superficial soil stratum. However, both elements remained far below levels considered phytotoxic. Nevertheless, further medium and long-term monitoring should be undertaken in order to evaluate the possible changes in soil of these trace elements.
Acknowledgements
The author is thankful to the Servicios Pucalán Sur Company for co-financing this experiment and to the SEMAMERIS Company for facilitating their installations in their Talca experimental station.