Effect of Iron Nanoparticles Synthesized by a Sol-Gel Process on Rhodococcus erythropolis T902.1 for Biphenyl Degradation


Nanoparticles (NPS) are considered as a new generation of compounds to improve environmental remediation and biological processes. The aim of this study is to investigate the effect of iron NPS encapsulated in porous silica (SiO2) on the biphenyl biodegradation by Rhodococcus erythropolis T902.1 (RT902.1). The iron NPS (major iron oxide FexOy form) were dispersed in the porosity of a SiO2 support synthesized by sol-gel process. These Fe/SiO2 NPS offer a stimulating effect on the biodegradation rate of biphenyl, an organic pollutant that is very stable and water-insoluble. This positive impact of NPS on the microbial biodegradation was found to be dependent on the NPS concentration ranging from 10-6 M to 10-4 M. After 18 days of incubation the cultures containing NPS at a concentration of 10-4 M of iron improved RT902.1 growth and degraded 35% more biphenyl than those without NPS (positive control) or with the sole SiO2 particles. Though the microorganism could not interact directly with the insoluble iron NPS, the results show that about 10% and 35% of the initial 10-4 M iron NPS encapsulated in the SiO2 matrix would be incorporated inside or adsorbed on the cell surface respectively and 35% would be released in the supernatant. These results suggest that RT902.1 would produce siderophore-like molecules to attract iron from the porous silica matrix.

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Wannoussa, W. , Masy, T. , Lambert, S. , Heinrichs, B. , Tasseroul, L. , Al-Ahmad, A. , Weekers, F. , Thonar, P. and Hiligsmann, S. (2015) Effect of Iron Nanoparticles Synthesized by a Sol-Gel Process on Rhodococcus erythropolis T902.1 for Biphenyl Degradation. Journal of Water Resource and Protection, 7, 264-277. doi: 10.4236/jwarp.2015.73021.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Indu Nair, C., Jayachandran, K. and Shashidhar, S. (2008) Biodegradation of Phenol. African Journal of Biotechnology, 7, 4951-4958.
[2] Watanabe, K. (2001) Microorganisms Relevant to Bioremediation. Current Opinion in Biotechnology, 12, 231-241.
[3] Bunescu, A., Besse-Hoggan, P., Sancelme, M., Mailhot, G. and Delort, A.M. (2008) Comparison of Microbial and Photochemical Processes and Their Combination for Degradation of 2-Aminobenzothiazole. Applied and Environmental Microbiology, 74, 2976-2984.
[4] De Windt, W., Aelterman, P. and Verstraete, W. (2005) Bioreductive Deposition of Palladium (0) Nanoparticles on Shewanella oneidensis with Catalytic Activity towards Reductive Dechlorination of Polychlorinated Biphenyls. Journal of Environmental Microbiology, 7, 314-325.
[5] Mergeay, M., Nies, D., Schlegel, H.G., Gerits, J., Charles, P. and van Gijsegem, F. (1985) Alcaligenes eutrophus CH34 Is a Facultative Chemolithotroph with Plasmid-Bound Resistance to Heavy Metals. Journal of Bacteriology, 162, 328-334.
[6] Sterritt, R.M. and Lester, J.N. (1980) Interactions of Heavy Metals with Bacteria. Science of the Total Environment, 14, 5-17.
[7] Yeom, S.H. and Yoo, Y.J. (1997) Overcoming the Inhibition Effects of Metal Ions in the Degradation of Benzene and Toluene by Alcaligenes xylosoxidans y234. Korean Journal of Chemical Engineering, 14, 204-208.
[8] Chun-Wei, K. and Barbara, R.S.G. (1996) Effect of Added Heavy Metal Ions 3-Chlorobenzoate in Anaerobic Bacterial Consortia. Applied and Environmental Microbiology, 62, 2317-2323.
[9] Kotresha, D. and Vidyasagar, G.M. (2008) Isolation and Characterization of Phenol Degrading Pseudomonas aeruginosa MTCC 4996. World Journal of Microbiology Biotechnology, 24, 541-547.
[10] Lin, C.W., Chen, S.Y. and Cheng, Y.W. (2006) Effect of Metals on Biodegradation Kinetics for Methyl tert-Butylether. Biochemical Engineering Journal, 32, 25-32.
[11] Nies, D.H. (1999) Microbial Heavy-Metal Resistance. Applied Microbiology and Biotechnology, 51, 730-750.
[12] Santos, E.C., Jacques, R.J.S., Bento, F.M., Peralba, M.C.R., Selbach, P.A., Sa, E.L. and Camargo, F.A. (2008) Anthracene Biodegradation and Surface Activity by an Iron-Stimulated Pseudomonas sp. Bioresource Technology, 99, 2644-2649.
[13] Chorao, C. (2008) Investigation of Rhodococcus rhodochrous Metabolism in Photo- and Bio-Degradation of 2-aminobenzothiazol: Effect of Cell Immobilisation and Role of Iron. Ph.D Thesis of University Blaise Pascal, Clermont-Ferrand.
[14] Zhang, W.X. (2003) Nanoscale Iron Particles for Environmental Remediation: An Overview. Journal of Nanoparticle Research, 5, 323-332.
[15] Ansari, F., Grigoriev, P., Libor, S., Tothill, I.E. and Ramsden, J.J. (2008) DBT Degradation Enhancement by Decorating Rhodococcus erythropolis IGST8 with Magnetic Fe3O4 Nanoparticles. Biotechnology and Bioengineering, 102, 1505-1512.
[16] Murugesan, K., Bokare, V., Jeon, J.R., Kim, E.J., Kim, J.H. and Chang, Y.S. (2011) Effect of Fe-Pd Bimetallic Nanoparticles on Sphingomonas sp. PH-07 and a Nano-Bio Hybrid Process for Triclosan Degradation. Bioresource Technology, 102, 6019-6025.
[17] Choi, M., Biswas, P., Fissan, H. and Pui, D.Y.H. (2003) Special Issue on Nanoparticles: Technology and Sustainabl Development. Journal of Nanoparticle Research, 5, 2-3.
[18] Wagner, S., Munzer, S., Behrens, P., Scheper, T., Bahnemann, D. and Kasper, C. (2009) Cytotoxicity of Titanium and Silicon Dioxide Nanoparticles. Journal of Physics: Conference Series, 170, 12-22.
[19] Pal, S., Tak, Y.K. and Song, J.M. (2007) Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli. Applied and Environmental Microbiology, 73, 1712-1720.
[20] Panyala, N.R., Pena-Mendez, E.M. and Havel, J. (2008) Silver or Silver Nanoparticles: A Hazardous Threat to the Environment and Human Health. Journal of Applied Biomedicine, 6, 117-129.
[21] Beckers, L., Hiligsmann, S., Lambert, S., Heinrichs, B. and Thonart, P. (2013) Improving Effect of Metal and Oxide Nanoparticles Encapsulated in Porous Silica on Fermentative Biohydrogen Production by Clostridium butyricum. Bioresource Technology, 133, 109-117.
[22] Lambert, S., Polard, J.F., Pirard, J.P. and Heinrichs, B. (2004) Improvement of Metal Dispersion in Pd/SiO2 Cogelled Xerogel Catalysts for 1, 2-dichloroethane Hydrodechlorination. Applied Catalysis B: Environmental, 50, 127-140.
[23] Lambert, S., Cellier, C., Grange, P., Pirard, J.P. and Heinrichs, B. (2004) Synthesis of Pd/SiO2, Ag/SiO2, and Cu/SiO2 Cogelled Xerogel Catalysts: Study of Metal Dispersion and Catalytic Activity. Journal of Catalysis, 221, 335-346.
[24] Lambert, S., Alie, C., Pirard, J.P. and Heinrichs, B. (2004) Study of Textural Properties and Nucleation Phenomenon in Pd/SiO2, Ag/SiO2 and Cu/SiO2 Cogelled Xerogel Catalysts. Journal of Non-Crystalline Solids, 342, 70-81.
[25] Marimuthu, T., Mohamad, S. and Alias, Y. (2014) Synthesis and Characterization of New Silica-Titania Mixed Oxide in the Presence of 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl) Imide by Sol-Gel Technique. Journal of Sol-Gel Science and Technology, 70, 104-110.
[26] Kaiser, A., Gorsmann, C. and Schubert, U. (1997) Influence of the Metal Complexation on Size and Composition of Cu/Ni Nano-Particles Prepared by Sol-Gel Processing. Journal of Sol-Gel Science and Technology, 8, 795-799.
[27] Heinrichs, B., Rebbouh, L., Geus, J.W., Lambert, S., Abbenhuis, H.C.L., Grandjean, F., Long, G.J., Pirard, J.P. and van Santen, R.A. (2008) Iron(III) Species Dispersed in Porous Silica through Sol-Gel Chemistry. Journal of Non-Crystalline Solids, 354, 665-672.
[28] Weekers, F., Jacques, P., Springael, D., Mergeay, M., Diels, L. and Thonart, Ph. (1999) Improving the Catabolic Functions of Desiccation-Tolerant Soil Bacteria. Applied Biochemistry and Biotechnology, 77, 251-266.
[29] Bunescu, A. (2006) Photo- and Bio-Degradation of Benzothiazole Compounds: Investigation of Combined Systems. Ph.D Thesis of University Blaise Pascal, Clermont-Ferrand.
[30] Bezkorovainy, A., Miller-Catchpole, R., Poch, M. and Solberg, L. (1986) The Mechanism of Iron Binding by Suspensions of Bifidobacterium bifidum var. Pennsylvanicus. Biochimica et Biophysica Acta (BBA)-General Subjects, 884, 60-66.
[31] O’Connor, K.E., Dobson, A.D. and Hartmans, S. (1997) Indigo Formation by Microorganisms Expressing Styrene Monooxygenase Activity. Applied and Environmental Microbiology, 63, 4287-4291.
[32] Nadaf, N.H. and Ghosh, J.S. (2011) Purification and Characterization of Catechol 1,2-dioxygenase from Rhodococcus sp. NCIM 2891. Research Journal of Environmental and Earth Sciences, 3, 608-613.
[33] Bradford, M.M. (1976) A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry, 72, 248-254.
[34] Lipczynska-Kochany, E., Harms, S., Milburn, R., Sprah, G. and Nadarajah, N. (1994) Degradation of Carbon Tetrachloride in the Presence of Iron and Sulphur Containing Compounds. Chemosphere, 29, 1477-1489.
[35] Kuony, S. (2005) Caracterisation of Arene Dioxygenase Involved in Biodegradation of Polycyclic Aromatic Hydrocarbons by Mycobacterium sp. 6pyl. Ph.D. Thesis, University Joseph Fourier, Grenoble.
[36] Bevinakatti, B.G. and Ninnekar, H.Z. (1992) Degradation of Biphenyl by a Micrococcus Species. Applied Microbiology and Biotechnology, 38, 273-275.
[37] Takeda, H., Yamada, A., Miyauchi, K., Masai, E. and Fukuda, M. (2004) Characterization of Transcriptional Regulatory Genes for Biphenyl Degradation in Rhodococcus sp. Strain RHA1. Journal of Bacteriology, 186, 2134-2146.
[38] Mahy, J.G., Tasseroul, L., Zubiaur, A., Geens, J., Brisbois, M., Herlitschke, M., Hermann, R., Heinrichs, B. and Lambert, S.D. (2014) Highly Dispersed Iron Xerogel Catalysts for p-Nitrophenol Degradation by Photo-Fenton Effects. Microporous and Mesoporous Materials, 197, 164-173.
[39] Olle, B., Bucak, S., Holmes, T.C., Bromberg, L., Hatton, T.A. and Wang, D.I.C. (2006) Enhancement of Oxygen Mass Transfer Using Functionalized Magnetic Nanoparticles. Industrial & Engineering Chemistry Research, 45, 4355-4363.
[40] Carrano, C.J., Jordan, M., Drechsel, H., Schmid, D.G. and Winkelmann, G. (2001) Heterobactins: A New Class of Siderophores from Rhodococcus erythropolis IGTS8 Containing both Hydroxamate and Catecholate Donor Groups. Biometals, 14, 119-125.
[41] Kraemer, S.M. (2004) Iron Oxide Dissolution and Solubility in the Presence of Siderophores. Aquatic Sciences, 66, 3-18.
[42] Kasemets, K., Ivask, A., Dubourguier, H.C. and Kahru, A. (2009) Toxicity of Nanoparticles of ZnO, CuO and TiO2 to Yeast Saccharomyces cerevisiae. Toxicology in Vitro, 23, 1116-1122.
[43] Pierre, J.L., Fontecave, M. and Crichton, R.R. (2002) Chemistry for an Essential Biological Process: The Reduction of Ferric Iron. Biometals, 15, 341-346.
[44] Candidus, S., Van Pee, K.H. and Ligens, F. (1994) The Catechol 2,3-dioxygenase Gene of Rhodococcus rhodochrous CTM: Nucleotide Sequence, Comparison with Isofunctional Dioxygenases and Evidences for an Active-Site Histidine. Microbiology, 140, 321-330.
[45] Furusawa, Y., Nagarajan, V., Tanokura, M., Masai, E., Fukuda, M. and Senda, T. (2004) Crystal Structure of the Terminal Oxygenase Component of Biphenyl Dioxygenase Derived from Rhodococcus sp. Strain RHA1. Journal of Molecular Biology, 342, 1041-1052.
[46] Zaki, S. (2006) Detection of Meta- and Ortho-Cleavage Dioxygenases in Bacterial Phenol-Degraders. Journal of Applied Sciences and Environmental Management, 10, 75-81.
[47] Dinkla, I.J.T., Gabor, E.M. and Janssen, D.B. (2001) Effects of Iron Limitation on Degradation of Toluene by Pseudomonas Strains Carrying the TOL (pWWO) Plasmid. Applied and Environmental Microbiology, 67, 3406-3412.
[48] Zheng, C., Huang, L., Xiu, J.L. and Huang, Z.Y. (2012) Investigation of a Hydrocarbon-Degrading Strain, Rhodococcus ruber Z25 for the Potential of Microbial Enhanced Oil Recovery. Journal of Petroleum Science and Engineering, 81, 49-56.

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