Growth Characteristic of the Oleaginous Mi-croalga Chlorella ellipsoidea SD-0701 with Lipid Accumulation

Abstract

Microalgae have great advantages as a new biomass source for fuel production. But microalgae are photosynthetic microorganisms, which normally grow in the light. Because of this growth condition, the commercial viability of microalgal biofuel is limited by current production systems. To obtain microalgal biofuel, fermentation is a more convenient, more economical and practical industry model. In this study, we asked whether and why the dark fermentation of C. ellipsoidea SD-0701 could be achieved by changing the culture medium formula. We focused the research on carbon-containing compounds and the initial pH of media. The results indicated that glucose was the optimum carbon-containing compound, which provided C. ellipsoidea SD-0701 with energy and carbon skeleton for accumulating organic compounds including lipids. When C. ellipsoidea SD-0701 was cultivated in the add-nutrition medium containing glucose, the optimum initial pH for the growth of C. ellipsoidea SD-0701 was pH 7.71. Therefore, if the suitable medium is used, C. ellipsoidea SD-0701 can grow normally in the dark, which is the same condition as the fermenter, and high microalgal biomass (0.50 g·L-1) and lipid yield (232.90 mg·L-1) can be achieved.

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Luo, W. , Du, W. , Su, Y. , Hui, J. , Zhuang, J. and Liu, L. (2015) Growth Characteristic of the Oleaginous Mi-croalga Chlorella ellipsoidea SD-0701 with Lipid Accumulation. Natural Resources, 6, 130-139. doi: 10.4236/nr.2015.62012.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Tushar, K.G. and Mark, A.P. (2011) Bioenergy. In: Energy Resources and Systems, 327-418.
[2] Herrmann, I.T., Jorgensen, A. and Bruun, S. (2012) Potential for Optimized Production and Use of Rapeseed Biodiesel. International Journal of Life Cycle Assessment, 18, 418-430.
http://dx.doi.org/10.1007/s11367-012-0486-8
[3] Tutunea, D. (2012) Thermal Investigation of Biodiesel Blends Derived from Rapeseed Oil. Journal of Thermal Analysis and Calorimetry, 111, 869-875.
http://dx.doi.org/10.1007/s10973-012-2213-x
[4] Firrisa, M.T., Iris, V.D. and Voinov, A. (2013) Energy Efficiency for Rapeseed Biodiesel Production in Different Farming Systems. Energy Efficiency, 7, 79-95.
http://dx.doi.org/10.1007/s12053-013-9201-2
[5] Kamzolova, S.V., Dedyukhina, E.G. and Samoilenko, V.A. (2013) Isocitric Acid Production from Rapeseed Oil by Yarrowia lipolytica Yeast. Applied Microbiology and Biotechnology, 97, 9133-9144.
http://dx.doi.org/10.1007/s00253-013-5182-5
[6] Kreivaitis, R., Gumbyte, M., Kazancev, K., Padgurskas, J. and Makarevitiene, V. (2013) A Comparison of Pure and Natural Antioxidant Modified Rapeseed Oil Storage Properties. Industrial Crops and Products, 43, 511-516.
http://dx.doi.org/10.1016/j.indcrop.2012.07.071
[7] Qi, D.H., Bae, C. and Feng, Y.M. (2013) Preparation, Characterization, Engine Combustion and Emission Characteristics of Rapeseed Oil Based Hybrid Fuels. Renew Energy, 60, 98-106.
http://dx.doi.org/10.1016/j.renene.2013.05.008
[8] Pessoa, F.L.P., Magalhaes, S.P. and Pedro, W.C.F. (2010) Production of Biodiesel via Enzymatic Ethanolysis of the Sunflower and Soybean Oils: Modeling. Applied Biochemistry and Biotechnology, 161, 238-244.
http://dx.doi.org/10.1007/s12010-009-8878-3
[9] Simasatitkul, L.P., Siricharnsakunchai, Patcharavorachot, Y., Assabumrungrat, S. and Arpornwichanop, A. (2011) Reactive Distillation for Biodiesel Production from Soybean Oil. Korean Journal of Chemical Engineering, 28, 649-655.
http://dx.doi.org/10.1007/s11814-010-0440-z
[10] Go, A.-R., Lee, Y., Kim, Y.H., et al. (2013) Enzymatic Coproduction of Biodiesel and Glycerol Carbonate from Soybean Oil in Solvent-Free System. Enzyme and Microbial Technology, 53, 154-158.
http://dx.doi.org/10.1016/j.enzmictec.2013.02.016
[11] Oliveira, S.O., Maruyama, S.A. and Claus, T. (2013) A Novel Response Surface Methodology Optimization of BaseCatalyzed Soybean Oil Methanolysis. Fuel, 113, 580-585.
http://dx.doi.org/10.1016/j.fuel.2013.06.011
[12] Wan, L., Liu, H. and Skala, D. (2014) Biodiesel Production from Soybean Oil in Subcritical Methanol Using MnCO3/ ZnO as Catalyst. Applied Catalysis B: Environmental, 152, 352-359.
http://dx.doi.org/10.1016/j.apcatb.2014.01.033
[13] Santos, A.G.D., Caldeira, V.P.S., Farias, M.F., Araujo, A.S., Souza, L.D. and Barros, A.K. (2011) Characterization and Kinetic Study of Sunflower Oil and Biodiesel. Journal of Thermal Analysis and Calorimetry, 106, 747-751.
http://dx.doi.org/10.1007/s10973-011-1838-5
[14] Tavares, M.L.A., Queiroz, N., Santos, I.M.G., Souza, A.L., Cavalcani, E.H.S., Barros, A.K.D., Rosenhaim, R., Soledade, L.E.B. and Souza, A.G. (2011) Sunflower Biodiesel Use of P-DSC in the Evaluation of Antioxidant Efficiency. Journal of Thermal Analysis and Calorimetry, 106, 575-579.
http://dx.doi.org/10.1007/s10973-011-1357-4
[15] Alenezi, R., Santos, R.C.D., Raymahasay, S. and Leeke, G.A. (2013) Improved Biodiesel Manufacture at Low Temperature and Short Reaction Time. Renewable Energy, 53, 242-248.
http://dx.doi.org/10.1016/j.renene.2012.11.019
[16] Sanchez, B.S., Mendow, G. and Levrand, P.G. (2013) Optimization of Biodiesel Production Process Using Sunflower Oil and Tetramethyl Ammonium Hydroxide as Catalyst. Fuel, 113, 323-330.
http://dx.doi.org/10.1016/j.fuel.2013.05.085
[17] Spinelli, D., Jez, S. and Pogni, R. (2013) Environmental and Life Cycle Analysis of a Biodiesel Production Line from Sunflower in the Province of Siena (Italy). Energy Policy, 59, 492-506.
http://dx.doi.org/10.1016/j.enpol.2013.04.009
[18] Min, J.Y. and Lee, E.Y. (2011) Lipase-Catalyzed Simultaneous Biosynthesis of Biodiesel and Glycerol Carbonate from Corn Oil in Dimethyl Carbonate. Biotechnology Letters, 33, 1789-1796.
http://dx.doi.org/10.1007/s10529-011-0627-3
[19] Raul, D., Jose, G.R. and Natalia, G. (2013) Energy Valorisation of Crude Glycerol and Corn Straw by Means of Slow Co-Pyrolysis: Production and Characterisation of Gas, Char and Bio-Oil. Fuel, 112, 31-37.
http://dx.doi.org/10.1016/j.fuel.2013.05.005
[20] Shi, A.M., Du, Z.Y. and Ma, X.C. (2013) Production and Evaluation of Biodiesel and Bioethanol from High Oil Corn Using Three Processing Routes. Bioresource Technology, 128, 100-106.
http://dx.doi.org/10.1016/j.biortech.2012.10.007
[21] Ciftci, O.N. and Temelli, F. (2014) Continuous Bioconversion of the Lipids of Corn, Wheat, and Triticale Distiller’s Dried Grains with Solubles to Biodiesel in Supercritical Carbon Dioxide and Characterization of the Products. BioEnergy Research, 7, 702-710.
http://dx.doi.org/10.1007/s12155-013-9394-4
[22] Silalertruksa, T. and Gheewala, S.H. (2012) Environmental Sustainability Assessment of Palm Biodiesel Production in Thailand. Energy, 43, 306-314.
http://dx.doi.org/10.1016/j.energy.2012.04.025
[23] Cho, H.J., Kim, J.K. and Ahmed, F. (2013) Life-Cycle Greenhouse Gas Emissions and Energy Balances of a Biodiesel Production from Palm Fatty Acid Distillate (PFAD). Applied Energy, 111, 479-488.
http://dx.doi.org/10.1016/j.apenergy.2013.05.038
[24] Liaquat, A.M., Masjuki, H.H. and Kalam, M.A. (2013) Impact of Palm Biodiesel Blend on Injector Deposit Formation. Applied Energy, 111, 882-893.
http://dx.doi.org/10.1016/j.apenergy.2013.06.036
[25] Khan, S.A., Rashmi, Hussain, M.Z., Prasad, S. and Banerjee, U.C. (2009) Prospects of Biodiesel Production from Microalgae in India. Renewable and Sustainable Energy Reviews, 13, 2361-2372.
http://dx.doi.org/10.1016/j.rser.2009.04.005
[26] Satyanarayana, K.G., Mariano, A.B. and Vargas, J.V.C. (2011) A Review on Microalgae, a Versatile Source for Sustainable Energy and Materials. International Journal of Energy Research, 35, 291-311.
http://dx.doi.org/10.1002/er.1695
[27] Nwachukwu, A.N. and Chukwu, M.A. (2012) The Potential of Macroalgae as a Source of Carbohydrates for Use in Bioethanol Fermentation. International Journal of Energy and Environmental, 3, 761-774.
[28] Wu, Y.H., Hu, H.Y., Yu, Y., Zhang, T.Y., Zhu, S.F., Zhuang, L.L., Zhang, X. and Lu, Y. (2014) Microalgal Species for Sustainable Biomass/Lipid Production Using Wastewater as Resource: A Review. Renewable and Sustainable Energy Reviews, 33, 675-688.
http://dx.doi.org/10.1016/j.rser.2014.02.026
[29] Gong, Y. and Jiang, M. (2011) Biodiesel Production with Microalgae as Feedstock: From Strains to Biodiesel. Biotechnology Letters, 33, 1269-1284.
http://dx.doi.org/10.1007/s10529-011-0574-z
[30] Burrows, E.H., Bennette, N.B., Carrieri, D., Dixon, J.L., Brinker, A., Frada, M., Baldassano, S.N., Falkowski, P.G. and Dismukes, G.C. (2012) Dynamics of Lipid Biosynthesis and Redistribution in the Marine Diatom Phaeodactylum tricornutum under Nitrate Deprivation. Biotechnology Letters, 5, 876-885.
http://dx.doi.org/10.1007/s12155-012-9201-7
[31] Gardner, R.D., Cooksey, K.E., Mus, F., Macur, R., Moll, K., Eustance, E., et al. (2012) Use of Sodium Bicarbonate to Stimulate Triacylglycerol Accumulation in the Chlorophyte Scenedesmus sp. and the Diatom Phaeodactylum tricornutum. Journal of Applied Phycology, 24, 1311-1320.
http://dx.doi.org/10.1007/s10811-011-9782-0
[32] Fields, M.W., Hise, A., Lohman, E.J., Bell, T., Gardner, R.D., Corredor, L., et al. (2014) Sources and Resources: Importance of Nutrients, Resource Allocation, and Ecology in Microalgal Cultivation for Lipid Accumulation. Journal of Applied Phycology, 98, 4805-4816.
http://dx.doi.org/10.1007/s00253-014-5694-7
[33] Luis, F.R. (2011) Net Energy Calculations for Production of Biodiesel and Biogas from Haematococcus pluvialis and Nannochloropsis sp. Green Energy and Technology, 83-91.
[34] Lei, A., Chen, H., Shen, G.M., Hu, Z.L., Chen, L. and Wang, J.X. (2012) Expression of Fatty Acid Synthesis Genes and Fatty Acid Accumulation in Haematococcus pluvialis under Different Stressors. Biotechnology for Biofuels, 5, 18.
http://dx.doi.org/10.1186/1754-6834-5-18
[35] Saha, S.K., McHugh, E. and Hayes, J. (2012) Effect of Various Stress-Regulatory Factors on Biomass and Lipid Production in Microalga Haematococcus pluvialis. Bioresource Technology, 128, 118-124.
http://dx.doi.org/10.1016/j.biortech.2012.10.049
[36] Gerhard, K. (2013) Production and Properties of Biodiesel from Microalgal Oils. Developments in Applied Phycology, 5, 207-221.
[37] Yuan, C., Liu, J.H., Fan, Y., Ren, X.H., Hu, G.R. and Li, F.L. (2011) Mychonastes afer HSO-3-1 as a Potential New Source of Biodiesel. Biotechnology for Biofuels, 4, 47.
http://dx.doi.org/10.1186/1754-6834-4-47
[38] Cheirsilp, B., Kitcha, S. and Torpee, S. (2011) Co-Culture of an Oleaginous Yeast Rhodotorula glutinis and a Microalga Chlorella vulgaris for Biomass and Lipid Production Using Pure and Crude Glycerol as a Sole Carbon Source. Annals of Microbiology, 62, 987-993.
http://dx.doi.org/10.1007/s13213-011-0338-y
[39] Pribyl, P., Cepak, V. and Zachleder, V. (2012) Production of Lipids in 10 Strains of Chlorella and Parachlorella, and Enhanced Lipid Productivity in Chlorella vulgaris. Applied Microbiology and Biotechnology, 94, 549-561.
http://dx.doi.org/10.1007/s00253-012-3915-5
[40] Talebi, A.F., Thidfar, M. and Tabatabaei, M. (2013) Genetic Manipulation, a Feasible Tool to Enhance Unique Characteristic of Chlorella vulgaris as a Feedstock for Biodiesel Production. Molecular Biology Reports, 40, 4421-4428.
http://dx.doi.org/10.1007/s11033-013-2532-4
[41] Al-Lwayzy, S.H., Yusaf, T. and Al-Juboori, R.A. (2014) Biofuels from the Fresh Water Microalgae Chlorella vulgaris (FWM-CV) for Diesel Engines. Energies, 7, 1829-1851.
http://dx.doi.org/10.3390/en7031829
[42] Demirbas, A. and Demirbas, M.F. (2010) Green Energy and Technology, Algae Energy, Algae as a New Source of Biodiesel. Springer, London, 139-157.
[43] Tang, H., Abunasser, N. and Garcia, M.E.D. (2011) Potential of Microalgae Oil from Dunaliella tertiolecta as a Feedstock for Biodiesel. Applied Energy, 88, 3324-3330.
http://dx.doi.org/10.1016/j.apenergy.2010.09.013
[44] Moheimani, N.R. (2012) Long-Term Outdoor Growth and Lipid Productivity of Tetraselmis suecica, Dunaliella tertiolecta and Chlorella sp (Chlorophyta) in Bag photobioreactors. Journal of Applied Phycology, 25, 167-176.
http://dx.doi.org/10.1007/s10811-012-9850-0
[45] Chow, Y.S., Goh, S.J.M., Su, Z.H., Ng, D.H.P., Lima, C.Y., Lim, N.Y.N., et al. (2013) Continual Production of Glycerol from Carbon Dioxide by Dunaliella tertiolecta. Bioresource Technology, 136, 550-555.
http://dx.doi.org/10.1016/j.biortech.2013.03.040
[46] Mario, R. and Tredici (2010) Photobiology of Microalgae Mass Cultures: Understanding the Tools for the Next Green Revolution. BioFuels, 1, 143-162.
[47] Dismukes, G.C., Carrieri, D., Bennette, N., Ananyev, G.M. and Posewitz, M.C. (2008) Aquatic Phototrophs: Efficient Alternatives to Land-Based Crops for Biofuels. Current Opinion in Biotechnology, 19, 235-240.
http://dx.doi.org/10.1016/j.copbio.2008.05.007
[48] Perego, C. and Ricci, M. (2012) Diesel Fuel from Biomass. Catalysis Science & Technology, 2, 1776-1786.
http://dx.doi.org/10.1039/c2cy20326j
[49] Demirbas, A. (2010) Biodiesel from Oilgae, Biofixation of Carbon Dioxide by Microalgae: A Solution to Pollution Problems. Applied Energy, 88, 3541-3547.
http://dx.doi.org/10.1016/j.apenergy.2010.12.050
[50] Liang, S.Z., Liu, X.M., Chen, F. and Chen, Z.J. (2004) Current Microalgal Health Food R & D Activities in China. Hydrobiologia, 512, 45-48.
http://dx.doi.org/10.1023/B:HYDR.0000020366.65760.98
[51] Pauline, S., Claire, J.C., Elie, D. and Arsène, I. (2006) Commercial Applications of Microalgae. Journal of Bioscience and Bioengineering, 101, 87-96.
http://dx.doi.org/10.1263/jbb.101.87
[52] Pienkos, P.T. and Darzins, A.L. (2009) The Promise and Challenges of Microalgal-Derived Biofuels. Biofuels, Bioproducts and Biorefining, 3, 431-440.
http://dx.doi.org/10.1002/bbb.159
[53] Rodolfi, L., Chini Zittelli, G., Bassi, N., Padovani, G., Biondi, N., Bonini, G. and Tredici, M.R. (2008) Microalgae for Oil: Strain Selection, in Duction of Lipid Synthesis and Outdoor Mass Cultivation in a Low-Cost Photobioreactor. Biotechnology and Bioengineering, 102, 100-112.
http://dx.doi.org/10.1002/bit.22033
[54] Zhang, C.M., Pan, W.B. and Chen, Y.Z. (2009) The Toxic Effects of the Extracellular Active Components from One Algae-Lysing Bacteria on Chlorella pyrenoidosa. Microbiology, 36, 821-825.
[55] Ma, S., Fu, L.L., Wang, M., Hu, X.W., Tan, D.G. and Zhang, J.M. (2010) Comparison of Extraction Methods for Crude Fat from Microalgae. China Oils and Fats, 35, 77-79.
[56] Gunnel, A. and Leonardo, M. (1991) Lipid Analysis of Freshwater Microalgae: A Method Study. Archiv Fur Hydrobiologie, 121, 295-306.
[57] Zheng, H.L., Gao, Z., Zhang, Q. and Huang, H. (2011) Effect of Inorganic Carbon Source on Lipid Production Withautotrophic Chlorella vulgaris. Chinese Journal of Biotechnology, 27, 436-444.
[58] Xue, Y.L. (1985) Determination of Soluble Sugar in the Plant Material. In: Xue, Y.L., Ed., Lab Manual of Plant Physiology, 134-138.
[59] Huang, J.F. and Zhang, Y.X. (2006) Influence of Citrate on Growth and Metabolism of CHO Cells. Journal of East China University of Science and Technology (Natural Science Edition), 32, 556-560.
[60] Cheng, L.H., Zhang, L., Chen, H.L. and Gao, C.K. (2005) Advances on CO2 Fixation by Microalgae. Chinese Journal of Biotechnology, 1, 177-181.

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