Comparative Study of Microbial Activities and Biodegradation-Abilities of Undefined Consortium in Some Hydrocarbon Contaminated Sites in the Niger Delta, Nigeria

O. O. Olanipekun; A. O. Ogunbayo; S. C. U. Nwachukwu; R. A. Bello

doi:10.4236/jep.2015.62016

Home > Journals > Earth & Environmental Sciences > JEP

JEP> Vol.6 No.2, February 2015

Share This Article:

Open Access

Comparative Study of Microbial Activities and Biodegradation-Abilities of Undefined Consortium in Some Hydrocarbon Contaminated Sites in the Niger Delta, Nigeria

Abstract Full-Text HTML XML

Download as PDF (Size:1009KB) PP. 138-145

DOI: 10.4236/jep.2015.62016 2,899 Downloads 3,359 Views Citations

Author(s) Leave a comment

O. O. Olanipekun^1*, A. O. Ogunbayo¹, S. C. U. Nwachukwu², R. A. Bello¹

Affiliation(s)

¹Department of Chemical Engineering, University of Lagos, Yaba, Lagos.
²Department of Microbiology, University of Lagos, Yaba, Lagos.

ABSTRACT

The microbial activities and the biodegradation-abilities of undefined consortium in contaminated soils in the Niger Delta of Nigeria were studied. The Respirametry technique was adopted to evaluate the microbial activities while the soils were incubated with 2% (υ/υ) crude oil in mineral salt medium at 37°;C in three stages of two weeks each in a shake flask. At the end of the last phase, components of the crude oil degraded by the undefined consortium in the soils were identified with the gas chromatographic techniques. The consortia of the different samples studied showed different degree of capacities on the crude oil, removing a large number of components of the crude oil, making the areas potentially suitable for in-situ bioremediation.

KEYWORDS

Microbial Activities, Undefined Consortium, Crude Oil, In-Situ, Bioremediation

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Olanipekun, O. , Ogunbayo, A. , Nwachukwu, S. and Bello, R. (2015) Comparative Study of Microbial Activities and Biodegradation-Abilities of Undefined Consortium in Some Hydrocarbon Contaminated Sites in the Niger Delta, Nigeria. Journal of Environmental Protection, 6, 138-145. doi: 10.4236/jep.2015.62016.

References

[1]	Obire, O. and Anyanwu, E.C. (2009) Impact of Various Concentration of Crude Oil on Fungal Populations of Soil. International Journal of Environmental Science and Technology, 6, 211-218. http://dx.doi.org/10.1007/BF03327624

[2]	Bossert, I.D. and Compeau, G.C. (1995) Cleanup Contamination in Soil.

[3]	Margesin, R., Walder, G. and Schminer, F. (2000) The Impact of Hydrocarbon Remediation (Diesel Oil and Polycyclic Aromatic Hydrocarbon) on Enzyme Activities and Microbial Properties of Soil. Acta Biotechnology, 20, 313-333. http://dx.doi.org/10.1002/abio.370200312

[4]	Leeson, A. and Hinchee, R.E. (1997) Soil Bioventing, Principles and Practice. CRC, Lewis Publishers, Boca Raton.

[5]	Margesin, R., Labbe, D., Schinner, F., Greer, C.W. and Whyte, L.G. (2003) Characterization of Hydrocarbon-Degrading Microbial Populations in Contaminated and Pristine Alpine Soils. Applied and Environmental Microbiology, 69, 3085-3092. http://dx.doi.org/10.1128/AEM.69.6.3085-3092.2003

[6]	Balb, M.T., Al-Awadhi, N. and Al-Daher, R. (1998) Bioremediation of Soil-Contaminated Soil: Microbiological Methods for Feasibility Assessment and Field Evacuation. Journal of Microbiological Methods, 32, 155-164. http://dx.doi.org/10.1016/S0167-7012(98)00020-7

[7]	Malik, Z.A. and Ahmed, S. (2012) Degradation of Petroleum Hydrocarbons by Oil Field Isolated Bacterial Consortium. African Journal of Biotechnology, 11, 650-658.

[8]	Forster, C. and Wase, J. (1989) Environmental Biotechnology. Ellis Horwood Publishers, England, 49-51.

[9]	Olanipekun, O.O., Ogunbayo, A.O. and Layokun, S.K. (2012) A Study of the Ability of Pseudomonas Aeruginosa and Pseudomonas fluorescens to Degrade Diesel Oil. JETEAS, 3, 429-434.

[10]	Uzoamaka, G.O., Floretta, T. and Florence, M.O. (2009) Hydrocarbon Degradation Potentials of Indigenous Fungal Isolates from Petroleum Contaminated Soils. J. Phy.Nat.Scs., 3, 1-6.

[11]	Eze, V.C. and Eze, B.N. (2010) Isolation and Characterization of Microorganisms Involved in the Degradation of Refined Petroleum Products Polluted Sites in Elele, Rivers State Nigeria. International Journal of Current Research, 8, 91-95.

[12]	Critter, S.A.M, Freitas, S.S. and Airobi, C. (2004) Comparison of Microbial Activity in Some Brazilian Soils by Microcalorimetre and Respirometric Methods. Thermochimica Acta, 410, 35-46. http://dx.doi.org/10.1016/S0040-6031(03)00371-X

[13]	Omotayo, A.E., Ojo, O.Y. and Amund, O.O. (2012) Crude oil Degradation by Microorganisms in Soil Composts. Research Journal of Microbiology, 7, 209-218. http://dx.doi.org/10.3923/jm.2012.209.218

[14]	Malatova, K. (2005) Isolation and Characterization of Hydrocarbon Degrading Bacteria from Environmental Habitats in Western New York State. A Thesis of Master of Science, Department of Chemistry, Rochester.