Physicochemical Properties of Rhamnolipid Biosurfactant from Pseudomonas aeruginosa PA1 to Applications in Microemulsions

DOI: 10.4236/jbnb.2015.61007   PDF   HTML   XML   5,929 Downloads   7,006 Views   Citations


Interfacial properties rhamnolipids from an extract produced by a strain of Pseudomonas aeruginosa were analyzed in this study. The extract of rhamnolipid was characterized by surface tension in different conditions; interfacial tension with different hydrocarbons; critical micelle concentration under different pH and temperatures; particle size and emulsification capacity using laser light profiling. It was observed that the rhamnolipids extract are sensitive to variations in pH, thermostable and function as good emulsificant for emulsification of methyl methacrylate. The emulsion stability order in function of the oil phase was methyl methacrylate > emulsions of castor oil > emulsion n-heptane > emulsion toluene > emulsion hexadecane > octane emulsion. The data presented show that rhamnolipid extracts may be used to formulate stable emulsions of methyl methacrylate. This process can be used to do nano/microsphere of polymethyl methacrylate.

Share and Cite:

Mendes, A. , Filgueiras, L. , Pinto, J. and Nele, M. (2015) Physicochemical Properties of Rhamnolipid Biosurfactant from Pseudomonas aeruginosa PA1 to Applications in Microemulsions. Journal of Biomaterials and Nanobiotechnology, 6, 64-79. doi: 10.4236/jbnb.2015.61007.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Soberón-Chávez, G., Lépine, F. and Dézie, E. (2005) Production of Rhamnolipids by Pseudomonas aeruginosa. Applied Microbiology and Biotechnology, 68, 718-725.
[2] Gudiña, E.J., Rodrigues, A.I., Alves, E., Domingues, M.R., Teixeira, J.A. and Rodrigues, L.R. (2014) Bioconversion of Agroindustrial By-Products in Rhamnolipids toward Applications in Enhanced Oil Recovery and Bioremediation. Bioresource Technology, 177C, 87-93.
[3] Maier, R.M. and Soberón-Chávez, G. (2000) Pseudomonas aeruginosa Rhamnolipids: Biosynthesis and Potential Applications. Applied Microbiology and Biotechnology, 54, 625-633.
[4] Stanghellini, M.E. and Miller, R.M. (1997) Biosurfactants: Their Identity and Potential Efficacy in the Biological Control of Zoosporic Plant Pathogens. Plant Disease, 81, 4-12.
[5] Guo, Y.P., Hu, Y.Y., Gu, R.R. and Lin, H. (2009) Characterization and Micellization of Rhamnolipidic Fractions and Crude Extracts Produced by Pseudomonas aeruginosa Mutant MIG-N146. Journal of Colloid and Interface Science, 331, 356-363.
[6] Déziel, E., Lépine, F., Milot, S. and Villemur, R. (2003) rhlA Is Required for the Production of a Novel Biosurfactant Promoting Swarming Motility in Pseudomonas aeruginosa: 3-(3-hydroxyalkanoyloxy) Alkanoic Acids (HAAs), the Precursors of Rhamnolipids. Microbiology, 149, 2005-2013.
[7] Abdel-Mawgoud, A.M., Lépine, F. and Déziel, E. (2010) Rhamnolipids: Diversity of Structures, Microbial Origins and Roles. Applied Microbiology and Biotechnology, 86, 1323-1336.
[8] Samadi, N., Abadian, N., Ahmadkhaniha, R., Amini, F., Dalili, D., Rastkari, N., Safaripour, E. and Mohseni, F.A. (2012) Structural Characterization and Surface Activities of Biogenic Rhamnolipid Surfactants from Pseudomonas aeruginosa Isolate MN1 and Synergistic Effects against Methicillin-Resistant Staphylococcus aureus. Folia Microbi- ologica (Praha), 57, 501-8.
[9] Mata-Sandoval, J.C., Karns, J. and Torrents, A. (1999) High-Performance Liquid Chromatography Method for the Characterization of Rhamnolipid Mixtures Produced by Pseudomonas aeruginosa UG2 on Corn Oil. Journal of Chro- matography A, 864, 211-220.
[10] Liu, Y., Zhong, H., Liu, Z., Jiang, Y., Tan, F., Zeng, G., Lai, M. and He, Y. (2014) Purification and Characterization of the Biosurfactant Rhamnolipid. Se Pu, 32, 248-255.
[11] Déziel, E., Lépine, F., Dennie, D., Boismenu, D., Mamer, O.A. and Villemur, R. (1999) Liquid Chromatography/Mass Spectrometry Analysis of Mixtures of Rhamnolipids Produced by Pseudomonas aeruginosa Strain 57RP Grown on Mannitol or Naphthalene. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1440, 244-252.
[12] Dar, A.A., Rather, G.M., Ghosh, S. and Das, A.R. (2008) Micellization and Interfacial Behavior of Binary and Ternary Mixtures of Model Cationic and Nonionic Surfactants in Aqueous NaCl Medium. Journal of Colloid and Interface Science, 322, 572-581.
[13] Zhang, Y.M., Maier, W.J. and Miller, R.M. (1997) Effect of Rhamnolipids on the Dissolution, Bioavailability, and Biodegradation of Phenanthrene. Environmental Science Technology, 31, 2211-2217.
[14] Shin, K.H., Kim, K.W. and Seagren, E.A. (2004) Combined Effects of pH and Biosurfactant Addition on Solubilization and Biodegradation of Phenanthrene. Applied Microbiology and Biotechnology, 65, 336-343.
[15] Itoh, S. and Suzuki, T. (1972) Effect of Rhamnolipids on Growth of Pseudomonas aeruginosa Mutant Deficient in n-Paraffin-Utilizing Ability. Agricultural and Biological Chemistry, 36, 2233-2235.
[16] Koch, A.K., Käppeli, O., Fiechter, A. and Reiser, J. (1991) Hydrocarbon Assimilation and Biosurfactant Production in Pseudomonas aeruginosa Mutants. Journal of Bacteriology, 173, 4212-4219.
[17] Kronemberger, F.D., Santa-Anna, L., Fernandes, A., Menezes, R., Borges, C. and Freire, D. (2008) Oxygen Controlled Biosurfactant Production in a Bench Scale Bioreactor. Applied Biochemistry and Biotechnology, 147, 33-45.
[18] Dubois, M., Gilles, K., Hamilton, J.K., Rebers, P.A. and Smith, F. (1951) A Colorimetric Method for the Determination of Sugars. Nature, 168, 167.
[19] Mendes, A.N., Hubber, I., Siqueira, M., Barbosa, G.M., de Lima Moreira, D., Holandino, C., Pinto, J.C. and Nele, M. (2012) Preparation and Cytotoxicity of Poly(Methyl Methacrylate) Nanoparticles for Drug Encapsulation. Macromolecular Symposia, 319, 34-40.
[20] Saien, J. and Akbari, S. (2006) Interfacial Tension of Toluene + Water + Sodium Dodecyl Sulfate from (20 to 50) °C and pH between 4 and 9. Journal of Chemical & Engineering Data, 51, 1832-1835.
[21] Ilori, M.O., Amobi, C.J. and Odocha, A.C. (2005) Factors Affecting Biosurfactant Production by Oil Degrading Aeromonas spp. Isolated from a Tropical Environment. Chemosphere, 61, 985-992.
[22] Inakollu, S., Hung, H.C. and Shreve, G.S. (2004) Biosurfactant Enhancement of Microbial Degradation of Various Structural Classes of Hydrocarbon in Mixed Waste Systems. Environmental Engineering Science, 21, 463-469.
[23] Lebrón-Paler, A., Pemberton, J.E., Becker, B.A., Otto, W.H., Larive, C.K. and Maier, R.M. (2006) Determination of the Acid Dissociation Constant of the Biosurfactant Monorhamnolipid in Aqueous Solution by Potentiometric and Spectroscopic Methods. Analytical Chemistry, 78, 7649-7658.
[24] Lovaglio, R.B., dos Santos, F.J., Jafelicci-Junior, M. and Contiero, J. (2011) Rhamnolipid Emulsifying Activity and Emulsion Stability: pH Rules. Colloids and Surfaces B: Biointerfaces, 85, 301-305.
[25] Sánchez, M., Aranda, F.J., Espuny, M.J. and Marqués, A. (2007) Aggregation Behavior of a Dirhamnolipid Biosurfactant Secreted by Pseudomonas aeruginosa in Aqueous Media. Journal of Colloid and Interface Science, 307, 246-253.
[26] Pornsunthorntawee, O., Chavadej, S. and Rujiravanit, R. (2011) Characterization and Encapsulation Efficiency of Rhamnolipid Vesicles with Cholesterol Addition. Journal of Bioscience and Bioengineering, 112, 102-106.
[27] Pornsunthorntawee, O., Chavadej, S. and Rujiravanit, R. (2009) Solution Properties and Vesicle Formation of Rhamnolipid Biosurfactants Produced by Pseudomonas aeruginosa SP4. Colloids and Surfaces B: Biointerfaces, 72, 6-15.
[28] Bai, G., Brusseau, M.L. and Miller, R.M. (1997) Influence of a Rhamnolipid Biosurfactant on the Transport of Bacteria through a Sandy Soil. Applied and Environmental Microbiology, 63, 1866-1873.
[29] Ishigami, Y., Gama, Y., Ishii, F. and Choi, Y.K. (1993) Colloid Chemical Effect of Polar Head Moieties of a Rhamnolipid-Type Biosurfactant. Langmuir, 9, 1634-1636.
[30] Helvaci, S.S., Peker, S. and Özdemir, G. (2004) Effect of Electrolytes on the Surface Behavior of Rhamnolipids R1 and R2. Colloids and Surfaces B: Biointerfaces, 35, 225-233.
[31] Nitschke, M., Costa, S.G.V.A.O. and Contiero, J. (2005) Rhamnolipid Surfactants: An Update on the General Aspects of These Remarkable Biomolecules. Biotechnology Progress, 21, 1593-1600.
[32] Wang, Q., Fang, X., Bai, B., Liang, X., Shuler, P.J., Goddard, W.A., et al. (2007) Engineering Bacteria for Production of Rhamnolipid as an Agent for Enhanced Oil Recovery. Biotechnol Bioeng, 98, 842-853.
[33] Nguyen, T.T., Edelen, A., Neighbors, B. and Sabatini, D.A. (2010) Biocompatible Lecithin-Based Microemulsions with Rhamnolipid and Sophorolipid Biosurfactants: Formulation and Potential Applications. Journal of Colloid and Interface Science, 348, 498-504.
[34] Urum, K., Grigson, S., Pekdemir, T. and McMenamy, S. (2006) A Comparison of the Efficiency of Different Surfactants for Removal of Crude Oil from Contaminated Soils. Chemosphere, 62, 1403-1410.
[35] Patel, P.A., Chaulang, G.M., Akolkotkar, A., Mutha, S.S., Hardikar, S.R. and Bhosale, A.V. (2008) Self Emulsifying Drug Delivery System: A Review. Research Journal of Pharmacy and Technology, 1, 313-323.
[36] Schork, F.J., Luo, Y., Smulders, W., Russum, J.P., Butté, A. and Fontenot, K. (2005) Miniemulsion Polymerization. Advances in Polymer Science, 175, 129-255.
[37] Mengual, O., Meunier, G., Cayre, I., Puech, K. and Snabre, P. (1999) Characterisation of Instability of Concentrated Dispersions by a New Optical Analyser: The TURBISCAN MA 1000. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 152, 111-123.
[38] Costa, S.G.V.A.O., Nitschke, M., Lépine, F., Déziel, E. and Contiero, J. (2010) Structure, Properties and Applications of Rhamnolipids Produced by Pseudomonas aeruginosa L2-1 from Cassava Wastewater. Process Biochemistry, 45, 1511-1516.

comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.