Gold Nanoparticle and Berberine Entrapped into Hydrogel Matrix as Drug Delivery System

DOI: 10.4236/jbnb.2015.61006   PDF   HTML   XML   4,990 Downloads   6,207 Views   Citations


In this study the novel hydrogel loaded with gold nanoparticle (AuNP) enhanced the berberine (BS) release when compared with other formulations of hydrogel. Hydrogels are hydrophilic polymer networks having the capacity to absorb water, ranging from about twenty to thousand times their dry weight. BS is a natural product, a quaternary ammonium salt from the group of isoquinoline alkaloids found in medicinal plants as Berberis Vulgaris. BS has some activity against dysentery, hypertension, inflammation, and liver disease in China and Japan. In this work, BS was used as a model drug to study its association with different types of hydrogel composites of polyvinyl alcohol (BS-PVA 10%); gellan gum (BS-GG 2%), gellan gum-PVA crosslinked with cysteine (cys) (BSGG2%PVA2%cys) and gellan gum-PVA cosslinked with cysteine associated with gold nanoparticles (AuNP-BSGG2%PVA2%cys). Several parameters such as fraction of retained water (Wf), hydration percentage (%H), Swelling (DSw) and time course profile (t = 100%) (TC) were evaluated for all preparations. The results showed that the AuNP-BS-GG2%PVA2%cys was able to retain more water and swelling than the other preparations. The time course of release of the BS to the medium was greater for AuNP-BS-GG2%PVA2%cys making it a candidate to drug delivery studies in biological assays. Also Scanning Electron Microscopy (SEM) images of the surface of these hydrogel were performed. Furthermore, crosslink of the resulting hydrogels were investigated by Fourier Transform Infrared Spectroscopy (FTIR) and differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). Thus, briefly, the aim of this work was to study three composition of hydrogel loaded with BS and its composition in relation to addition to AuNP and evaluate its profile for further drug delivery application using the Surface Plasmonic Resonance (SPR) as a tool improving the drug release in the new hydrogel.

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Souza, C. , Oliveira, H. , Pinheiro, W. , Biswaro, L. , Azevedo, R. , Gomes, A. and Lunardi, C. (2015) Gold Nanoparticle and Berberine Entrapped into Hydrogel Matrix as Drug Delivery System. Journal of Biomaterials and Nanobiotechnology, 6, 53-63. doi: 10.4236/jbnb.2015.61006.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Huang, Z.J., Zeng, Y., Lan, P., Sun, P.H. and Chen, W.M. (2011) Advances in Structural Modifications and Biological Activities of Berberine: An Active Compound in Traditional Chinese Medicine. Mini-Reviews in Medicinal Chemistry, 11, 1122-1129.
[2] Tillhon, M., Guamán Ortiz, L.M., Lombardi, P. and Scovassi, A.I. (2012) Berberine: New Perspectives for Old Remedies. Biochemical Pharmacologyhem Pharmacol, 84, 1260-1267.
[3] Xie, W. and Du, L. (2011) Diabetes Is an Inflammatory Disease: Evidence from Traditional Chinese Medicines. Diabetes, Obesity and Metabolism, 13, 289-301.
[4] Wang, X.H., Jiang, S.M. and Sun, Q.W. (2011) Effects of Berberine on Human Rheumatoid Arthritis Fibroblast-Like Synoviocytes. Experimental Biology and Medicine (Maywood), 236, 859-866.
[5] Chen, X.W., Di, Y.M., Zhang, J., Zhou, Z.W., Li, C.G. and Zhou, S.F. (2012) Interaction of Herbal Compounds with Biological Targets: A Case Study with Berberine. Scientific World Journal, 2012, Article ID: 708292.
[6] Chen, Y., Wang, Y., Zhang, J., Sun, C. and Lopez, A. (2011) Berberine Improves Glucose Homeostasis in Streptozotocin-Induced Diabetic Rats in Association with Multiple Factors of Insulin Resistance. ISRN Endocrinology, 2011, Article ID: 519371.
[7] Chidambara Murthy, K.N., Jayaprakasha, G.K. and Patil, B.S. (2012) The Natural Alkaloid Berberine Targets Multiple Pathways to Induce Cell Death in Cultured Human Colon Cancer Cells. European Journal of Pharmacology, 688, 14- 21.
[8] Tan, W., Li, Y., Chen, M. and Wang, Y. (2011) Berberine Hydrochloride: Anticancer Activity and Nanoparticulate Delivery System. International Journal of Nanomedicine, 6, 1773-1777.
[9] Trimarco, V., Cimmino, C.S., Santoro, M., Pagnano, G., Manzi, M.V., Piglia, A., et al. (2012) Nutraceuticals for Blood Pressure Control in Patients with High-Normal or Grade 1 Hypertension. High Blood Pressure & Cardiovascular Prevention, 19, 117-122.
[10] Wang, J., Liu, Q. and Yang, Q. (2012) Radiosensitization Effects of Berberine on Human Breast Cancer Cells. International Journal of Molecular Medicine, 30, 1166-1172.
[11] Wang, Y., Huang, Y., Lam, K.S., Li, Y., Wong, W.T., Ye, H., et al. (2009) Berberine Prevents Hyperglycemia-Induced Endothelial Injury and Enhances Vasodilatation via Adenosine Monophosphate-Activated Protein Kinase and Endothelial Nitric Oxide Synthase. Cardiovascular Research, 82, 484-492.
[12] Zhou, L., Wang, X., Yang, Y., Wu, L., Li, F., Zhang, R., et al. (2011) Berberine Attenuates cAMP-Induced Lipolysis via Reducing the Inhibition of Phosphodiesterase in 3T3-L1 Adipocytes. Biochimica et Biophysica Acta (BBA)—Mole- cular Basis of Disease, 1812, 527-535.
[13] Minhas, M.U., Ahmad, M., Ali, L. and Sohail, M. (2013) Synthesis of Chemically Cross-Linked Polyvinyl Alcohol-co- Poly (methacrylic acid) Hydrogels by Copolymerization; A Potential Graft-Polymeric Carrier for Oral Delivery of 5- Fluorouracil. DARU Journal of Pharmaceutical Sciences, 21.
[14] Zhang, X.Z., Zhuo, R.X., Cui, J.Z. and Zhang, J.T. (2002) A Novel Thermo-Responsive Drug Delivery System with Positive Controlled Release. International Journal of Pharmaceutics, 235, 43-50.
[15] Liu, L., Wang, B., Gao, Y. and Bai, T.C. (2013) Chitosan Fibers Enhanced Gellan Gum Hydrogels with Superior Mechanical Properties and Water-Holding Capacity. Carbohydrate Polymers, 97, 152-158.
[16] Moura, M.J., Faneca, H., Lima, M.P., Gil, M.H. and Figueiredo, M.M. (2011) In Situ Forming Chitosan Hydrogels Prepared via Ionic/Covalent Co-Cross-Linking. Biomacromolecules, 12, 3275-3284.
[17] Cencetti, C., Bellini, D., Pavesio, A., Senigaglia, D., Passariello, C., Virga, A., et al. (2012) Preparation and Characterization of Antimicrobial Wound Dressings Based on Silver, Gellan, PVA and Borax. Carbohydrate Polymers, 90, 1362-1370.
[18] Cobley, C.M., Chen, J., Cho, E.C., Wang, L.V. and Xia, Y. (2011) Gold Nanostructures: A Class of Multifunctional Materials for Biomedical Applications. Chemical Society Reviews, 40, 44-56.
[19] Bedford, E.E., Spadavecchia, J., Pradier, C.M. and Gu, F.X. (2012) Surface Plasmon Resonance Biosensors Incorporating Gold Nanoparticles. Macromolecular Bioscience, 12, 724-739.
[20] Cristallini, C., Guerra, G.D., Barbani, N. and Bianchi, F. (2007) Biodegradable Bioartificial Materials Made with Chitosan and Poly(vinyl alcohol). Part I: Physicochemical Characterization. Journal of Applied Biomaterials & Biomechanics, 5, 184-191.
[21] Cascone, M.G., Barbani, N., Cristallini, C., Giusti, P., Ciardelli, G. and Lazzeri, L. (2001) Bioartificial Polymeric Materials Based on Polysaccharides. Journal of Biomaterials Science, Polymer Edition, 12, 267-281.
[22] Oliveira, J.T., Martins, L., Picciochi, R., Malafaya, P.B., Sousa, R.A., Neves, N.M., et al. (2010) Gellan Gum: A New Biomaterial for Cartilage Tissue Engineering Applications. Journal of Biomedical Materials Research Part A, 93, 852- 863.
[23] Kimling, J., Maier, M., Okenve, B., Kotaidis, V., Ballot, H. and Plech, A. (2006) Turkevich Method for Gold Nanoparticle Synthesis Revisited. Journal of Physical Chemistry B, 110, 15700-15707.
[24] Cai, X., Shao, W., Luan, Y., Pang, J., Li, F. and Li, Z. (2011) Metformin Hydrochloride-Loaded Poly(vinyl alcohol) Composites as Drug Delivery Systems. Journal of Nanoscience and Nanotechnology, 11, 8621-8627.
[25] Chen, C.H., Wang, F.Y., Mao, C.F., Liao, W.T. and Hsieh, C.D. (2008) Studies of Chitosan: II. Preparation and Characterization of Chitosan/Poly(vinyl alcohol)/Gelatin Ternary Blend Films. International Journal of Biological Macromolecules, 43, 37-42.
[26] Lima, A.C., Sher, P. and Mano, J.F. (2012) Production Methodologies of Polymeric and Hydrogel Particles for Drug Delivery Applications. Expert Opinion on Drug Delivery, 9, 231-248.
[27] Hu, Y., Wang, Q. and Tang, M. (2013) Preparation and Properties of Starch-g-PLA/Poly(vinyl alcohol) Composite Film. Carbohydrate Polymers, 96, 384-388.
[28] Krauland, A.H., Leitner, V.M. and Bernkop-Schnürch, A. (2003) Improvement in the in Situ Gelling Properties of Deacetylated Gellan Gum by the Immobilization of Thiol Groups. Journal of Pharmaceutical Sciences, 92, 1234-1241.
[29] Bashmakova, N., Kutovyy, S., Kornienko, M., Yashchuk, V., Hovorun, D. and Zhurakivsky, R. (2010) Experimental and Calculated by the DFT Method Vibration Spectra of Berberine. Xxii International Conference on Raman Spectroscopy, 1267, 426-427.
[30] Ahuja, M., Singh, S. and Kumar, A. (2013) Evaluation of Carboxymethyl Gellan Gum as a Mucoadhesive Polymer. International Journal of Biological Macromolecules, 53, 114-121.
[31] Coutinho, D.F., Sant, S.V., Shin, H., Oliveira, J.T., Gomes, M.E., Neves, N.M., et al. (2010) Modified Gellan Gum Hydrogels with Tunable Physical and Mechanical Properties. Biomaterials, 31, 7494-7502.
[32] Liu, Y., Geever, L.M., Kennedy, J.E., Higginbotham, C.L., Cahill, P.A. and McGuinness, G.B. (2010) Thermal Behavior and Mechanical Properties of Physically Crosslinked PVA/Gelatin Hydrogels. Journal of the Mechanical Behavior of Biomedical Materials, 3, 203-209.
[33] Peng, Z., Kong, L.X., Li, S.D. and Spiridonov, P. (2006) Poly(vinyl alcohol)/Silica Nanocomposites: Morphology and Thermal Degradation Kinetics. Journal of Nanoscience and Nanotechnology, 6, 3934-3938.
[34] Wang, H.Y., Lu, S.S. and Lun, Z.R. (2009) Glass Transition Behavior of the Vitrification Solutions Containing Propanediol, Dimethyl Sulfoxide and Polyvinyl Alcohol. Cryobiology, 58, 115-117.

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