Preliminary Study on the Histology in the Head Sarcoma of Goldfish (Carassius auratus)

Lei Wang¹, Zaizhong Chen¹, Caixia Hu¹, Feng Yan², Jun Xu², Xuehua Wang², Yuanyuan Zhou², Wei Wang², Weihua Zhao²

¹College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, China.
²Shanghai Pudong Ornamental Fish Center, Shanghai, China.

DOI: 10.4236/ojas.2015.51006                

Abstract

The sarcoma structure of goldfish was first analyzed by frozen section technique. The present study revealed the alkaline and acid phosphatase distribution in the head sarcoma of goldfish. And the histochemical staining in situ by calcium-cobalt method and lead nitrate method displayed a large amount of alkaline and acid phosphatases in the head sarcoma, with higher enzyme activity. The alkaline and acid phosphatases belong to hydrolases, which are widely present in various tissues. The results showed that the activity of external sarcoma is similar to the middle one, while there was more alkaline phosphatase near to the tissue sarcoma under the goldfish scales. And they have the strong activity. The study will lay the theoretical basis on enhancing the ornamental value of goldfish.

Keywords

Goldfish, Head Sarcoma, Frozen Sectioning Method, AKP, ACP

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Liang, Q.J. (1995) The Origin and Evolution of Goldfish. Biology Bulletin, 30, 14-16.
[2]	Liang, Q.J., Peng, Y.X. and Yu, Q.M. (1998) The Judgment Analysis and Cluster Analysis between Wild Crucian Carp and Five Varieties of Goldfish. Journal of Aquatic Biology, 23, 236-242.
[3]	Shi, Z.Y., Li, Y. and Xie, X.Z. (2008) Relations between Alkalinity Phosphatase and Calcium Metabolism in Mantle of Hyriopsis cumingii. Journal of Shanghai Fisheries University, 17, 291-297.
[4]	Sun, J.Q., Xu, Y., Zhang, H.Q. and Ye, J.Q. (2007) Study on Cytochemical Location of Acid Phosphatase and Alkaline Phospatase of Litopenaeus vannamei. Journal of Fudan University (Natural Science), 46, 947-951.
[5]	Wang, J.F. and He, Y.M. (2004) Experiment of Cell Biology. Science Press, Beijing, 61-62.
[6]	Wang, H.Y. (2000) Atlas of Chinese Goldfish. Science Press, Beijing, 2-6.
[7]	Zhang, W., Su, Y.Q., Wang, J., Quan, C.G. and Liang, J.R. (2001) Biochemical Composition of Five Common Reared Fishes. Marine Science Bulletin, 20, 26-31.
[8]	Wang, A.L., Liu, J.H. and Wang, W.N. (2005) Comparative Studies on the Total Pigment and Pigment Components of Color Carp. Acta Hydrobiologica Sinica, 29, 694-698.
[9]	Spinelli, J. and Mahnken, C. (1978) Carotenoid Deposition in Pen-Reared Salmonids Fed Diets Containing Oil Extracts of Red Crab (Pleuroncodes planipes). Aquaculture, 13, 2l3-223. http://dx.doi.org/10.1016/0044-8486(78)90003-0
[10]	Sun, H.S. and Li, G.Y. (1999) The Activities and Their Electron Microscopic Cytochemistry Study of AKP and ACP in Haemolymph of Chlamys farreri. Journal of Fishery Sciences of China, 6, 6-9.
[11]	Pan, D.R. (2007) Cell Biology. Science Press, Beijing, 136-137.