Interaction between Live Yeast and Dietary Rumen Degradable Protein Level: Effects on Diet Utilization in Early-Lactating Dairy Cows

DOI: 10.4236/as.2015.61001   PDF   HTML   XML   3,924 Downloads   4,665 Views   Citations


Four early lactating Holstein cows were used to study the effect of live yeast (LY, Actisaf® CNCM I-4407, Lesaffre Feed Additives, Marcq en Baroeul, France) supplementation on diet digestive utilization of dairy cows receiving concentrated corn silage-based diets with two rumen-degradable protein (RDP) levels. For a 33 d period, cows were fed a total mixed ration (TMR) containing an adequate level (AL) of RDP or a low level (LL, 30% below AL) by using soybean meal or tanned soybean meal, respectively: for 21 d with no LY addition followed by 12 d during which LY was added to the diet. The pH and redox potential (Eh) were recorded and ruminal fluid samples were collected over 3 consecutive days. Feces were collected individually over 48 h and individual dry matter intake (DMI) was measured for determining apparent nutrient digestibility. The effective degradability of individual feed ingredients composing both diets was evaluated with nylon bags technique. Structure of the ruminal bacterial community was studied and diversity index was calculated. Digestibility of organic matter (OM) and crude protein (CP) were lower for LL than those for AL. With LY, digestibility of OM and CP was increased: +2.4 and +0.8 points, for AL, and +3.7 and +5.9 points for LL, respectively. Live yeast reduced dietary N ruminal degradation with both AL and LL. Ruminal pH and Eh were lower with AL compared to LL: 5.95 and –167 mV vs. 6.13 and –144 mV. Live yeast increased ruminal total volatile fatty acids (VFA) (+8.6%), C2 (+10%), and C4 (+35%) contents for LL and decreased that of C3 (?9.8%) for AL. Neither the structure of bacterial populations of the rumen nor the diversity index (Shannon) was altered by treatments. Those results suggested a specific interest in using LY in RDP deficient diets for early lactating cows.

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Julien, C. , Marden, J. , Auclair, E. , Moncoulon, R. , Cauquil, L. , Peyraud, J. and Bayourthe, C. (2015) Interaction between Live Yeast and Dietary Rumen Degradable Protein Level: Effects on Diet Utilization in Early-Lactating Dairy Cows. Agricultural Sciences, 6, 1-13. doi: 10.4236/as.2015.61001.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Jones, D.F., Hoover, W.H. and Miller Webster, T.K. (1998) Effects of Concentrations of Peptides on Microbial Metabolism in Continuous Culture. Journal of Animal Science, 76, 611-616.
[2] Campanile, K.A., Zicarelli, F., Vecchio, D., Pacelli, C., Neglia, G., Balestrieri, A., Di Palo, R. and Infascelli, F. (2008) Effects of Saccharomyces cerevisiae on in Vivo Organic Matter Digestibility and Milk Yield in Buffalo Cows. Livestock Science, 114, 358-361.
[3] Marden, J.P., Julien, C., Monteils, V., Auclair, E., Moncoulon, R. and Bayourthe, C. (2008) How Does Live Yeast Differ from Sodium Bicarbonate to Stabilize Ruminal pH in High Yielding Dairy Cows? Journal of Dairy Science, 91, 3528-3535.
[4] Wang, C., Liu, Q., Yang, W.Z., Dong, Q., Yang, X.M., He, D.C., Zhang, P., Dong, K.H. and Huang, Y.X. (2009) Effects of Selenium Yeast on Rumen Fermentation, Lactation Performance and Feed Digestibilities in Lactating Dairy cows. Livestock Science, 126, 239-244.
[5] Chademana, I. and Offer, N.W. (1990) The Effect of Dietary Inclusion of Yeast Culture on Digestion in the Sheep. Animal Production, 50, 483-489.
[6] Williams, P.E.V., Tait, C.A.G., Innes, G.M. and Newbold, C.J. (1991) Effects of the Inclusion of Yeast Culture (Saccharomyces cerevisiae plus Growth Medium) in the Diet of Dairy Cows on Milk Yield and Forage Degradation and Fermentation Patterns in the Rumen of Steers. Journal of Animal Science, 69, 3016-3026.
[7] Moloney, A.P. and Drennan, M.J. (1994) The Influence of the Basal Diet on the Effects of Yeast Culture on Ruminal Fermentation and Digestibility in Steers. Animal Feed Science and Technology, 50, 55-73.
[8] Miranda, R.L.A., Mendoza, G., Barcena-Gama, R., Gonzalez, M.S.S., Ferrara, R., Ortega, M.E. and Cobos, P.M.A. (1996) Effect of Saccharomyces cerevisiae or Aspergillus oryzae Cultures and NDF Level on Parameters of Ruminal Fermentation. Animal Feed Science and Technology, 63, 289-296.
[9] Lascano, G.J. and Heinrichs, A.J. (2009) Rumen Fermentation Pattern of Dairy Heifers Fed Restricted Amounts of Low, Medium, and High Concentrate Diets without and with Yeast Culture. Livestock Science, 124, 48-57.
[10] Lascano, G.J., Zanton, G.I. and Heinrichs, A.J. (2009) Concentrate Levels and Saccharomyces cerevisiae Affect Rumen Fluid-Associated Bacteria Numbers in Dairy Heifers. Livestock Science, 126, 189-194.
[11] Chaucheyras-Durand, F., Walker, N.D. and Bach, A. (2008) Effects of Active Dry Yeasts on the Rumen Microbial Ecosystem: Past, Present and Future. Animal Feed Science and Technology, 145, 5-26.
[12] Sniffen, C.J., Chaucheyras-Durand, F., De Ondarza, M.B. and Donaldson, G. (2004) Predicting the Impact of a Live Yeast Strain on Rumen Kinetics and Ration Formulation. Proceedings of the 19th Annual Southwest Nutrition and Management Conference, Tempe, 24-25 February 2004, 53-59.
[13] Giger-Reverdin, S., Bezault, N., Sauvant, D. and Bertin, G. (1996) Effects of a Probiotic Yeast in Lactating Ruminants: Interaction with Dietary Nitrogen Level. Animal Feed Science and Technology, 63, 149-162.
[14] Streeter, M.N., Wagner, D.G., Hibberd, C.A. and Owens, F.N. (1990) Comparison of Corn with Four Sorghom Grain Hybrids: Site and Extent of Digestion in Steers. Journal of Animal Science, 68, 3429-3440.
[15] NRC (2001) Nutrient Requirements of Dairy Cattle. 7th Revised Edition, National Academy of Sciences, Washington DC.
[16] Julien, C., Marden, J.P., Bonnefont, C., Moncoulon, R., Auclair, E., Monteils, V. and Bayourthe, C. (2010) Effects of Varying Proportions of Concentrates on Ruminal-Reducing Power and Bacterial Community Structure in Dry Dairy Cows Fed Hay-Based Diets. Animal, 4, 1641-1646.
[17] Orskov, E.R. and McDonald, I. (1979) Estimation of Protein Degradability in the Rumen from Incubation Measurements Weighted According to Rate of Passage. Journal of Agricultural Science, 92, 499-503.
[18] Playne, M.J. (1985) Determination of Ethanol, Volatile Fatty Acids, Lactic Acid and Succinic Acid in Fermentation Liquids by Gas Chromatography. Journal of the Science of Food and Agriculture, 36, 638-644.
[19] Verdouv, H., Van Echteld, C.J.A. and Dekkers, E.M.J. (1978) Ammonia Determination Based on Indophenol Formation with Sodium Salicylate. Water Research, 12, 399-402.
[20] Krom, M.D. (1980) Spectrophotometric Determination of Ammonia: A Study of a Modified Berthelot Reaction Using Salicylate and Dichloroisocyanurate. Analyst, 105, 305-316.
[21] Houba, V.J.G., Novozamsky, I., Uittenbogaard, J. and Van Der Lee, J.J. (1987) Automatic Determination of Total Soluble Nitrogen in Soil Extracts. Landwirtschaftliche Forschung, 40, 295-302.
[22] George, S.K., Dipu, M.T., Mehra, U.R., Singh, P., Verma, A.K. and Ramgaokar, J.S. (2006) Improved HPLC Method for the Simultaneous Determination of Allantoin, Uric Acid and Creatinine in Cattle Urine. Journal of Chromatography B, 832, 134-137.
[23] Delbès, C., Godon, J.J. and Moletta, R. (1998) 16S rDNA Sequence Diversity of a Culture-Accessible Part of an Anaerobic Digestor Bacterial Community. Anaerobe, 4, 267-275.
[24] Zumstein, E., Moletta, R. and Godon, J.J. (2000) Examination of Two Years of Community Dynamics in an Anaerobic Bioreactor Using Fluorescence Polymerase Chain Reaction (PCR) Single-Strand Conformation Polymorphism Analysis. Environmental Microbiology, 2, 69-78.
[25] Michelland, R.J., Monteils, V., Zened, A., Combes, S., Cauquil, L., Gidenne, T., Hamelin, J. and Fortun-Lamothe, L. (2009) Spatial and Temporal Variations of the Bacterial Community in the Bovine Digestive Tract. Journal of Applied Microbiology, 107, 1642-1656.
[26] Michelland, R.J., Dejean, S., Combes, S., Fortun-Lamothe, L. and Cauquil, L. (2009) StatFingerprints: A Friendly Graphical Interface Program for Processing and Analysis of Microbial Fingerprint Profiles. Molecular Ecology Resources, 9, 1359-1363.
[27] R Development Core Team (2009) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna.
[28] Marounek, M., Roubal, P. and Bartos, S. (1987) The Redox Potential, rH and pH Values in the Gastrointestinal Tract of Small Ruminants. Physiologia Bohemoslovaca, 36, 71-74.
[29] Valadares, R.F.D., Broderick, G.A., Valadares Filho, S.C. and Clayton, M.K. (1999) Effect of Replacing Alfalfa Silage with High Moisture Corn on Ruminal Protein Synthesis Estimated from Excretion of Total Purine Derivatives. Journal of Dairy Science, 82, 2686-2696.
[30] Sauvant, D., Meschy, F. and Mertens, D.R. (1999) Les composantes de l’acidose ruminale et les effets acidogènes des rations. INRA Productions Animales, 12, 49-60.
[31] Sauvant, D., Giger-Reverdin, S. and Meschy, F. (2006) Le contr?le de l’acidose ruminale latente. INRA Productions Animales, 19, 69-78.
[32] Lechartier, C. and Peyraud, J.L. (2010) The Effects of Forage Proportion and Rapidly Degradable Dry Matter from Concentrate on Ruminal Digestion in Dairy Cows Fed Corn Silage-Based Diets with Fixed Neutral Detergent Fiber and Starch Contents. Journal of Dairy Science, 93, 666-681.
[33] Hristov, A.N., Etter, R.P., Ropp, J.K. and Grandeen, K.L. (2004) Effect of Dietary Crude Protein Level and Degradability on Ruminal Fermentation and Nitrogen Utilization in Lactating Dairy Cows. Journal of Animal Science, 82, 3219-3229.
[34] Gressley, T.F. and Armentano, L.E. (2007) Effects of Low Rumen-Degradable Protein or Abomasal Fructan Infusion on Diet Digestibility and Urinary Nitrogen Excretion in Lactating Dairy Cows. Journal of Dairy Science, 90, 1340-1353.
[35] Kalscheur, K.F., Baldwin, R.L., Glenn, B.P. and Kohn, R.A. (2006) Milk Production of Dairy Cows Fed Differing Concentrations of Rumen-Degraded Protein. Journal of Dairy Science, 89, 249-259.
[36] Reynal, S.M. and Broderick, G.A. (2005) Effect of Dietary Level of Rumen-Degraded Protein on Production and Nitrogen Metabolism in Lactating Dairy Cows. Journal of Dairy Science, 88, 4045-4064.
[37] Satter, L.D. and Slyter, L.L. (1974) Effect of Ammonia Concentration on Rumen Microbial Protein Production in Vitro. British Journal of Nutrition, 22, 199-208.
[38] Hoover, W.H. (1986) Chemical Factors Involved in Ruminal Fiber Digestion. Journal of Dairy Science, 69, 2755-2766.
[39] Russell, J.B. and Hino, T. (1985) Regulation of Lactate Production in Streptococcus bovis: A Spiraling Effect That Contributes to Rumen Acidosis. Journal of Dairy Science, 68, 1712-1721.
[40] Johnson, L.M., Harrison, J.H. and Riley, R.E. (1998) Estimation of the Flow of Microbial Nitrogen to the Duodenum Using Urinary Uric or Allantoin. Journal of Dairy Science, 81, 2408-2420.
[41] Peyraud, J.L., Le Liboux, S. and Vérité, R. (1997) Effet du niveau et de la nature de l’azote dégradable sur la digestion ruminale d’un régime à base d’ensilage de ma?s chez la vache laitière. Reproduction Nutrition Development, 37, 313-328.
[42] Monteils, V., Rey, M. and Gidenne, T. (2009) Mid to Long Term Stability of Ruminal Physicochemistry in Dairy Cows Fed a Fibre- or a Starch-Based Diet. Proceedings of the 11th International Symposium on Ruminant Physiology, Clermont-Ferrand, 6-9 September 2009, 284-285.
[43] Loisel, P., Harmand, J., Zemb, O., Latrille, E., Lobry, C., Delgenes, J.P. and Godon, J.J. (2006) Denaturing Gradient Electrophoresis (DGE) and Single-Strand Conformation Polymorphism (SSCP) Molecular Fingerprintings Revisited by Simulation and Used as a Tool to Measure Microbial Diversity. Environmental Microbiology, 8, 720-731.
[44] Guedes, C.M., Gon?alves, D., Rodrigues, M.A.M. and Dias-da-Silva, A. (2008) Effects of a Saccharomyces cerevisiae Yeast on Ruminal Fermentation and Fibre Degradation of Maize Silages in Cows. Animal Feed Science and Technolohy, 145, 27-40.
[45] Julien, C., Marden, J.P., Enjalbert, F., Bayourthe, C. and Troegeler-Meynadier, A. (2010) Live Yeast as a Possible Modulator of Polyunsaturated Fatty Acid Biohydrogenation in the Rumen. Revue Médecine Vétérinaire, 161, 391-400.
[46] Pinloche, E., McEvan, N., Marden, J.P., Bayourthe, C., Moncoulon, R. and Newbold, C.J. (2008) Effect of Yeast on the Bacterial Diversity of the Rumen. Proceedings of the 6th INRA-RRI Symposium of Gut Microbiology, Clermont-Ferrand, 18-20 June 2008, 9.
[47] Monteils, V., Cauquil, L., Auclair, E. and Bayourthe, C. (2006) Behaviour of Live Yeast BIOSAF Sc 47 during Digestive Transit in Dairy Cows. Proceedings of the 4th INRA-RRI Symposium of Gut Microbiology, Aberdeen, 21-23 June 2006, S106-S107.

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