Comparative Studies on Egyptian and Libyan Roselle Seeds as a Source of Lipid and Protein


Proceeding from the fact that the seeds of Roselle plant are full of nutritional constituents, however in Egypt and Libya, they are often discarded as a by-product, this study aims to evaluate the nutritional composition of Roselle seeds grown in Egypt and Libya as a source of oil and protein besides making a comparison between whole chemical composition of Roselle seeds grown in both countries. Ground of whole Egyptian and Libyan Roselle seeds powder contained high amount of protein (31.02% ± 0.93% and 28.67% ± 0.45%), crude fat (21.6% ± 0.66% and 16.94% ± 0.86%) and total ash (6.89% ± 0.11% and 5.60% ± 0.10%), respectively. However, Egyptian seeds have moisture content, protein, crude fat and total ash higher than Libyan seeds. Crude oil from Egyptian seeds had high refractive index and iodine value in comparison with crude oil from Libyan seeds. There were no remarkable differences between both seeds in acidity percent, unsaponifiable matters percent and saponification value. Linoleic, oleic and palmitic acids were the major fatty acid constituents in Egyptian Roselle seeds. Meanwhile linolenic, linoleic, oleic, stearic, palmitoleic and palmitic acids were the major fatty acid constituents in Libyan Roselle seeds. Crude oil from Egyptian seeds had higher percent of unsaturated fatty acids than crude oil from Libyan seeds. Unsaponifiable matters constituents for extracted oil from Egyptian seeds were free from n-pentacosane (C25) and rich in n-hexacosane (C26). Oil from both seeds had the same content of Beta sito-sterol and stigma-sterol. Both seeds were rich in glutamic acid, aspartic acid, arginine and leucine. Libyan seeds were rich in essential amino acids in comparison with Egyptian seeds. Finally nutritional comparison of Roselle seeds variation depends on the variety, location and environmental conditions during cultivation. Roselle seeds are a good source for extraction of oil and protein. Protein from Roselle seeds could be used as a supplement material for poor food in lysine.

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Elneairy, N. (2014) Comparative Studies on Egyptian and Libyan Roselle Seeds as a Source of Lipid and Protein. Food and Nutrition Sciences, 5, 2237-2245. doi: 10.4236/fns.2014.522237.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Barampama, Z. and Simard, R.E. (1993) Nutrient Composition, Protein Quality and Anti-Nutritional Factors of Some Varieties of Dry Beam (Phaseolus vulgaris) Grown in Burundi. Food Chemistry, 47, 159-167.
[2] Saleh Al-Jassir, M. (1992) Chemical Composition and Microflora of Black Cumin (Nigella sativa L.) Seeds Growing in Saudi Arabia. Food Chemistry, 45, 239-242.
[3] Akanbi, W.B., Olaniyan, A.B., Togun, A.O., Hupeju, A.E.O. and Alaniran, A.O. (2009) The Effect of Organic and Inorganic Fertilizer on Growth, Calyx Yield and Quality of Roselle (Hibiscus sabdriffa L.). American-Eurasian Journal of Sustainable Agriculture, 3, 652-657.
[4] Tsai, P.J., McIntosh, J., Pearce, P., Camden, B. and Jordan, B.R. (2002) Anthocyanin and Antioxidant Capacity in Roselle (Hibiscus sabdariffa L.) Extract. Food Research International, 35, 351-356.
[5] Chewonarin, T., Kinouchi, T., Katuoka, K., Arimachi, H., Kuwahara, T. and Initkekumnuen, U. (1999) Effect of Roselle (Hibiscus sabdariffa Linn.), a Thai Medicinal Plant , on the Mutagenicity of Various Unknown Mutagens in Salmonella typhimurium and Formation of Aberrant Crypt Foci Induced by the Colon Carcinogens Azoxymethane and 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in F344 Rates. Food and chemical Toxicology, 37, 591-601.
[6] Abu-Tarbouch, H.M., Ahmed, S.A.B. and Alkahtani, H.A. (1997) Some Nutritional Properties of Karkade (Hibiscus sabdariffa) Seeds Product. Cereal Chemistry, 74, 352-355.
[7] Rao, P.U. (1996) Nutrient Composition and Biological Evaluation of Mesta (Hibiscus sabdariffa) Seeds. Plant Foods for Human Nutrition, 49, 27-34.
[8] Mohiuddin, M.M. and Zaidi, H.R. (1975) Composition and Characteristic of Hibiscus sabdriffa Seeds Oil. Fat, Soap and Cooling Materials, 77, 488-489.
[9] Morton, J. (1987) Roselle. In: Morton, J.F., Ed., Fruits of Warm Climates, Florida Flair Books, Miami, 281-286.
[10] Duke, J.A. (1983) Malvaceae Roselle. In: Handbook of Energy Crops, 345-369.
[11] Nyam, K., Leao, S., Tan, C. and Long, K. (2012) Functional Properties of Roselle (Hibiscus sabdriffa L.) Seeds and Its Application as Bakery Product. Journal of Food Science and Technology, 51, 3830-3837.
[12] Hainida, E., Amin, I., Normah, H., Mohd-Esa, N. and Anial, Z.A.B. (2008) Effects of Defatted Dried ROSELLE (Hibiscus sabdariffa L.) Seeds Powder on Lipid Profile of Hypercholesterolemia Rats. Journal of the Science of Food and Agriculture, 88, 1043-1050.
[13] El-Adawy, T.A. and Khalil, A.H. (1994) Characteristics of Roselle Seeds as a New Source of Protein and Lipid. Journal of Agricultural and Food Chemistry, 42, 1986-1900.
[14] AOAC (2000) Official Methods of Analysis of the Association of Official Analytical Chemists. 17th Edition, Washington DC.
[15] AOCS (2010) American Oil Chemists Society. Official Methods and Recommended Practices. American Oil Chemists Society, Champaign.
[16] Vogel, A.J. (1975) A Textbook of Practical Organic Chemistry. 3rd Edition, English Language Book Society and Longman Group Ltd., London, 969-971.
[17] Farag, R.S., Hallabo, S.A.S., Hewedi, F.M. and Basyony, A.E. (1986) Chemical Evaluation of Rapeseed. Fette, Seifen, Anstrichmittel, 88, 391-397.
[18] Cohen, S.A., Mewyes, M. and Travin, T.L. (1989) The Pico-Tag Method: A Manual of Advanced Techniques for Amino Acid Analysis. Millipore, Billerica.
[19] Steel, R., Torrie, J. and Dickey, D. (1997) Principles and Procedures of Statistics: A Biometrical Approach. 3rd Edition, McGraw-Hill, New York.
[20] Shaheen, M.A., El-Nakhlawy, F.S. and Al-Shareef, R.A. (2012) Roselle (Hibiscus sabdriffa) Seeds as Unconventional Nutritional Source. African Journal of Biotechnology, 44, 9821-9824.
[21] Nzikou, J.M., Bouanga-Kalou, G., Matos, L., Ganongo-po, F.B., Mboungou-Mboussi, P.S., Moutoula, F.E., PanyooAkdow, E., Silou, T.H. and Desobry, S. (2011) Characteristics and Nutritional Evaluation of Seeds Oil from Roselle (Hibiscus sabdriffa L.) in Congo-Brazzaville. Current Research Journal of Biological Sciences, 3, 141-146.
[22] Parkouda, C., Diawara, B. and Ouoba, L.I.I. (2008) Technology and Physico-Chemical Characteristics of Bikalga, Alkaline Fermented Seeds of Hibiscus sabdriffa. African Journal of Biotechnology, 7, 916-922.
[23] Yagoub, A. and Mohammed, M.A. (2008) Fururndu, a Meat Substitute from Fermented Roselle (Hibiscus sabdriffa L.) Seed: Investigation on Amino Acids Composition, Protein Fractions, Minerals Content and HCl-Extractability of Microbial Growth. Pakistan Journal of Nutrition, 7, 352-358.
[24] Yagoub, A.A., Mohammed, A.M. and Abu Baker, A.A. (2008) Effect of Soaking, Sprouting and Cooking on Chemical composition, Bioavailability of Minerals in Vitro Digestibility of Roselle (Hibiscus sabdriffa L.) Seed. Pakistan Journal of Nutrition, 7, 50-56.
[25] Awad, A.M., Selim, K.A. and Abdel-Baki, M.R. (2008) Physico-Chemical and Oxidative Stability Characteristics of Roselle Seeds Oil as By-Product. Egypt Journal of Applied Science, 23, 247-257.
[26] Abu-Tarboush, M.H. (1995) Factors Affecting Protein Extractability of Defatted Karkade (Hibiscus sabdriffa) Seeds Flour. College of Foods and Agricultural Science. J. King Saud Univ. Agric. Sci., 7, 179-186.
[27] Al-Wandawi, H., Al-Shaikhly, K. and Abdul-Rahman, M. (1984) Roselle Seed: A New Protein Source. Journal of Agricultural and Food Chemistry, 32, 510-512.
[28] Samy, M.S. (1980) Chemical and Nutritional Studies on Roselle Seeds (H. sabdriffa L.). Journal of Olea Science, 10, 32-38.
[29] Cissouma, A.I., Tounkara, F., Nikoo, M., Yang, N. and Xu, X. (2013) Physico-Chemical Properties and Antioxidant Activity of Roselle Seeds Extracts. Advance Journal of Food Science and Technology, 5, 1483-1489.
[30] Nakpong, P. and Wootthikanokkhan, S. (2010) Roselle (Hibiscus sabdriffa L.) Oil as an Alternative Feedstock for Biodiesel Production in Thailand. Fuel, 89, 1806-1811.
[31] Atta, M.B. and Imaizumi, K. (2002) Some Characteristics of Crude Oil Extracted from Roselle (Hibiscus sabdriffa L.) Seeds Cultivated in Egypt. Journal of Oleo Science, 51, 457-461.
[32] Mohamed, R., Fernandez, J., Pineda, M. and Aquilar, M. (2007) Roselle (Hibiscus sabdriffa) Seeds Oil Is a Rich Source of Gamma-Tocopherols. Journal of Food Science, 72, S207-S211.
[33] Fiad, S. (1991) Component Triacylglycerol of Six Seeds Oils of Malvaceae. Journal of the American Oil Chemists Society, 68, 23-25.
[34] Ismail, A., Emmy Hainida, K.I. and Nazri, H.S.A. (2008) Roselle (Hibiscus sabdriffa L.) Seed—Nutritional Composition, Protein Quality and Health Benefits. Global Science Books, Food, 2, 1-16.
[35] El Faki, A.E., Dirar, H.A., Collins, M.A. and Harper, D.B. (1991) Biochemical and Microbiology Investigation of Sigda—A Sudanese Fermented Food Derived from Sesame Seeds Oil Cake. Journal of the Science of Food and Agriculture, 57, 351-365.
[36] FAO/WHO (1975) Energy and Protein Requirement. PAG Bulletin 5, PAG, New York, 30-35.

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