Achieving Ultra-Low Detection Limit Using Nanofiber Labels for Rapid Disease Detection

Emmanuel Gikunoo^1*, Adeyabeba Abera², Eyassu Woldesenbet^2,3

¹Materials Engineering Department, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
²Mechanical Engineering Department, National Science Foundation Center for Next Generation Composites, Southern University and A & M College, Baton Rouge, USA.
³Mechanical Engineering Department, National Science Foundation Center for Next Generation Composites, Louisiana State University, Baton Rouge, USA.

DOI: 10.4236/aid.2014.44030                

Abstract

Early diagnosis of diseases is critical in its effective management. Traditional disease detection methods require specialized equipment and trained personnel. With the introduction of rapid diagnostic test kits (RDTs), disease detection has become easier and faster. However, these RDTs have failed to compete with the specialized laboratory equipment due to their high detection limits and false alarm rates. This paper presents a novel method of using carbon nanofibers (CNFs) grown on glass microballoons (NMBs) to achieve ultra-low detection limits in RDTs. The NMBs have millions of nanosized CNFs grown on each microballoon, with each CNF having a strong bonding affinity for antibodies. The NMBs conjugated with secondary antibodies have therefore a significantly higher probability of capturing minute antigen concentrations in solution. Furthermore, the dark color formation at the capture zone makes visual disease detection possible. Human Immunoglobulin G (IgG) was selected as the model analyte to study the performance of NMBs using a sandwich immunoassay protocol. Ultra-low electrical detection limit of (4 pg/ml) and rapid re- sponse (~1 minute) was achieved using this method.

Keywords

Rapid Diagnostic Test Kits, Detection Limit, Carbon Nanofibers, Immunoassay, Immunoglobulin G

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Chen, G. (2013) Fluorescence Biosensor for H5N1 Antibody Based on Metal-Organic Framework Platform. Journal of Materials Chemistry B, 1, 1812-1817. http://dx.doi.org/10.1039/c3tb00501a
[2]	Po-Yueh, W. and Lu, M.S.C. (2011) CMOS Thermal Sensor Arrays for Enzymatic Glucose Detection. Sensors Journal, IEEE, 11, 3469-3475. http://dx.doi.org/10.1109/JSEN.2011.2161283
[3]	Rayana, R.R.-A., Hugo Javier, S.-P., María Liliana, M.-G., Bernardo, A.F.-U. and Abel, M. (2011) Chemical Biosensors Based on Proteins Involved in Biomineralization Processes. In: Serra, P.A., Ed., Biosensors—Emerging Materials and Applications, InTech, Gwalior. http://www.intechopen.com/books/biosensors-emerging-materials-and-applications/chemical-biose nsors-based-on-proteins-involved-in-biomineralization-processes
[4]	Lu, Y., Peng, S., Luo, D. and Lal, A. (2011) Low-Concentration Mechanical Biosensor Based on a Photonic Crystal Nanowire Array. Nature Communications, 2, 578. http://dx.doi.org/10.1038/ncomms1587
[5]	Hua, W., Yan, C., Hassibi, A., Scherer, A. and Hajimiri, A. (2009) A Frequency-Shift CMOS Magnetic Biosensor Array with Single-Bead Sensitivity and No External Magnet. Solid-State Circuits Conference—Digest of Technical Papers, ISSCC 2009. IEEE International, 438-439.
[6]	Zhang, D.-W., Liu, J.-X., Nie, J., Zhou, Y.-L. and Zhang, X.-X. (2013) Micropipet Tip-Based Miniaturized Electroche- mical Device Combined with Ultramicroelectrode and Its Application in Immobilization-Free Enzyme Biosensor. Analytical Chemistry, 85, 2032-2036. http://dx.doi.org/10.1021/ac303223u
[7]	Turner, A.P. (2013) Biosensors: Sense and Sensibility. Chemical Society Reviews, 42, 3184-3196.http://dx.doi.org/10.1039/c3cs35528d
[8]	Childerhose, J.E. and Macdonald, M.E. (2013) Health Consumption as Work: The Home Pregnancy Test as a Domesticated Health Tool. Social Science & Medicine, 86, 1-8. http://dx.doi.org/10.1016/j.socscimed.2013.02.035
[9]	Lazcka, O., Campo, F.J.D. and Muñoz, F.X. (2007) Pathogen Detection: A Perspective of Traditional Methods and Biosensors. Biosensors and Bioelectronics, 22, 1205-1217. http://dx.doi.org/10.1016/j.bios.2006.06.036
[10]	Linares, E.M., Kubota, L.T., Michaelis, J. and Thalhammer, S. (2012) Enhancement of the Detection Limit for Lateral Flow Immunoassays: Evaluation and Comparison of Bioconjugates. Journal of Immunological Methods, 375, 264-270.http://dx.doi.org/10.1016/j.jim.2011.11.003
[11]	Abera, A. and Choi, J.W. (2010) Quantitative Lateral Flow Immunosensor Using Carbon Nanotubes as Label. Analytical Methods, 2, 1819-1822. http://dx.doi.org/10.1039/c0ay00412j
[12]	Fournier, P.E., Drancourt, M., Colson, P., Rolain, J.M., Scola, B.L., Raoult, D., Fournier, P.E., Drancourt, M., Colson, P., Rolain, J.M., Scola, B.L. and Raoult, D. (2013) Modern Clinical Microbiology: New Challenges and Solutions. Nature Reviews Microbiology, 11, 574-585. http://dx.doi.org/10.1038/nrmicro3068
[13]	Justino, C.I.L., Rocha-Santos, T.A.P., Duarte, A.C. and Rocha-Santos, T.A.P. (2013) Advances in Point-of-Care Technologies with Biosensors Based on Carbon Nanotubes. TrAC Trends in Analytical Chemistry, 45, 24-36. http://dx.doi.org/10.1016/j.trac.2012.12.012
[14]	Kim, J., Lee, J.Y., Jin, J.H., Park, C., Lee, C. and Min, N. (2012) A Fully Microfabricated Carbon Nanotube Three- Electrode System on Glass Substrate for Miniaturized Electrochemical Biosensors. Biomedical Microdevices, 14, 613-624. http://dx.doi.org/10.1007/s10544-012-9640-0
[15]	Mendes, R.G., Bachmatiuk, A., Büchner, B., Cuniberti, G. and Rümmeli, M.H. (2013) Carbon Nanostructures as Multi-Functional Drug Delivery Platforms. Journal of Materials Chemistry B, 1, 401-428. http://dx.doi.org/10.1039/c2tb00085g
[16]	Zegeye, E., Jin, Y. and Woldesenbet, E. (2012) A Paper Like Structure Formed by Binding Self-Assembled Glass Microballoons Using Random CNF Networks. Materials Letters, 68, 490-492. http://dx.doi.org/10.1016/j.matlet.2011.11.045
[17]	Gikunoo, E., Abera, A. and Woldesenbet, E. (2014) A Novel Carbon Nanofibers Grown on Glass Microballoons Immunosensor: A Tool for Early Diagnosis of Malaria. Sensors, 14, 14686-14699. http://dx.doi.org/10.3390/s140814686
[18]	Hermanson, G.T. (2008) Bioconjugate Techniques. 2nd Edition, Academic Press, London, 1323.
[19]	Rao, V.K., Suresh, S., Sharma, M.K., Gupta, A. and Vijayaraghavan, R. (2011) Carbon Nanotubes—A Potential Material for Affinity Biosensors. Nanotechnology and Nanomaterials Carbon Nanotubes—Growth and Applications. InTech, Gwalior. http://www.intechopen.com/books/carbon-nanotubes-growth-and-applications
[20]	Nguyen, L.Q., Phan, P.Q., Duong, H.N., Nguyen, C.D. and Nguyen, L.H. (2013) Enhancement of NH3 Gas Sensitivity at Room Temperature by Carbon Nanotube-Based Sensor Coated with Co Nanoparticles. Sensors, 13, 1754-1762. http://dx.doi.org/10.3390/s130201754
[21]	Marie, R., Beech, J.P., Vörös, J., Tegenfeldt, J.O. and Höök, F. (2006) Use of PLL-g-PEG in Micro-Fluidic Devices for Localizing Selective and Specific Protein Binding. Langmuir, 22, 10103-10108. http://dx.doi.org/10.1021/la060198m
[22]	Chen, R.J., Bangsaruntip, S., Drouvalakis, K.A., Kam, N.W.S., Shim, M., Li, Y., Kim, W., Utz, P.J. and Dai, H. (2003) Noncovalent Functionalization of Carbon Nanotubes for Highly Specific Electronic Biosensors. Proceedings of the National Academy of Sciences of the United States of America, 100, 4984-4989. http://dx.doi.org/10.1073/pnas.0837064100
[23]	Ding, Y., Li, D., Li, B., Zhao, K., Du, W., Zheng, J.Y. and Yang, M.H. (2013) A Water-Dispersible, Ferrocene-Tagged Peptide Nanowire for Amplified Electrochemical Immunosensing. Biosensors and Bioelectronics, 48, 281-286. http://dx.doi.org/10.1016/j.bios.2013.04.030
[24]	Jie, G., Li, L.L., Chen, C., Xuan, J. and Zhu, J.J. (2009) Enhanced Electrochemiluminescence of CdSe Quantum Dots Composited with CNTs and PDDA for Sensitive Immunoassay. Biosensors and Bio-electronics, 24, 3352-3358. http://dx.doi.org/10.1016/j.bios.2009.04.039
[25]	Liu, H., Wu, X.M., Zhang, X., Burda, C. and Zhu, J.J. (2011) Gold Nanoclusters as Signal Amplification Labels for Optical Immunosensors. The Journal of Physical Chemistry C, 116, 2548-2554. http://dx.doi.org/10.1021/jp206256j
[26]	Leng, C., Wu, J., Xu, Q.N., Lai, G.S., Ju, H.X. and Yan, F. (2011) A Highly Sensitive Disposable Immunosensor through Direct Electro-Reduction of Oxygen Catalyzed by Palladium Nanoparticle Decorated Carbon Nanotube Label. Biosensors and Bioelectronics, 27, 71-76. http://dx.doi.org/10.1016/j.bios.2011.06.017
[27]	Yang, Y.C., Dong, S.W., Shen, T., Jian, C.X., Chang, H.J., Li, Y. and Zhou, J.X. (2011) Amplified Immunosensing Based on Ionic Liquid-Doped Chitosan Film as a Matrix and Au Nanoparticle Decorated Graphene Nanosheets as Labels. Electrochimica Acta, 56, 6021-6025. http://dx.doi.org/10.1016/j.electacta.2011.04.096
[28]	Liu, G., Chen, H.D., Peng, H.Z., Song, S.P., Gao, J.M., Lu, J.X., et al. (2011) A Carbon Nanotube-Based High-Sensitivity Electrochemical Immunosensor for Rapid and Portable Detection of Clenbuterol. Biosensors and Bioelectronics, 28, 308-313. http://dx.doi.org/10.1016/j.bios.2011.07.037
[29]	Wang, Z., Gao, H. and Fu, Z. (2013) Introducing Novel Amorphous Carbon Nanoparticles as Energy Acceptors into a Chemiluminescence Resonance Energy Transfer Immunoassay System. Analyst, 138, 6753-6758.