Antimicrobial Resistance Profile of Salmonella Isolates from Livestock

Sarah I. Umeh^1*, Chika Paulinus Enwuru²

¹Department of Microbiology, Federal University of Technology, Owerri, Nigeria.
²Department of Medical Laboratory Sciences, Imo State College of Nursing and Health Sciences, Amaigbo, Nigeria.

DOI: 10.4236/ojmm.2014.44027                

Abstract

Food animals are important reservoirs of infectious pathogens. The use of antimicrobial drugs in food animals is a major source of selection of drug resistant pathogens. This study investigated a total of 1000 faecal samples of livestock and poultry between January 2012 and March 2013 to determine the prevalence rate of Salmonella species and their antimicrobial resistance profiles. Faecal samples of chicken, pig, cattle, goat and sheep (200 samples of each) were pre-enriched in Tetrathionate broth and Rappapport Vassiliadis R10 broth. The broth culture was subcultured on XLD agar and incubated. The isolates were identified by standard biochemical tests and confirmed by AP1 20E test kit. Antimicrobial susceptibility was performed by disk diffusion method. The result showed that the prevalence of Salmonella spp. in all the samples was 21.8%. Chicken faeces had the highest prevalence rate of 52.5% followed by pig faeces (40%), cattle (10%), goat (4.5%) and sheep (2%). The isolates have resistance profile ranging from 1 to 9 antimicrobial drugs. Tetracycline had the highest resistance (81%) of all the isolates followed by Streptomycin (68%). Gentamycin had the lowest resistance profile of 14%. We conclude that Salmonella species have high prevalence rate in chicken and pig, but less in cattle, goat and sheep, and that most of the isolates are resistant to most commonly used antibiotics. Effort is needed to adopt measures to control the spread of multidrug resistant pathogens to humans. Care must be taken in the use of antibiotics in farm animals to reduce the selection of multidrug resistant strains.

Keywords

Antimicrobials, Faeces, Livestock, Multidrug Resistance, Salmonella

Conflicts of Interest

The authors declare no conflicts of interest.

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