Ciprofloxacin Sensitivity of Staphylococcus Strains Isolated at the Sylvanus Olympio University Hospital, Togo

Mounerou Salou^1,2*, Degninou Yehadji³, Koumavi Ekouevi², Sika Dossim^2,4, Chantal Tsogou³, Yawo Tufa Nyasenu^1,2, Fiali Lack^1,2, Mireille Prince-David^1,2, Anoumou Y. Dagnra^1,2

¹Service de Microbiologie CHU Sylavanus Olympio, Lomé,Togo.
²Département des Sciences fondamentales de la Faculté des Sciences de la Santé ,Université de Lomé , Lomé, Togo.
³Ecole Supérieure des Techniques Biologiques et Alimentaires, Université de Lomé, Lomé,Togo.
⁴Service laboratoire CHU Campus, Lomé, Togo.

DOI: 10.4236/pp.2014.513124                

Abstract

Background: Staphylococcal infections are managed by hygiene measures and usage of antibiotics. The first-line treatment refers to beta-lactamins. However, the emergence of beta-lactamin resistant staphylococcal strains has been reported, as demonstrated by a study conducted in Lomé University Teaching Hospital, Bacteriology Laboratory (2001) on 742 Staphylococcus aureus strains which revealed 67.00% of them where methicillin resistant. In this setting of emergent methicillin-resistant strains, the second-line treatments are prescribed by clinicians without antibiograms. Fluoroquinolones are the first preference molecules used for second-line treatment because of their efficacy and affordability. We want to contribute to setting monitoring and alert-making tools for drug prescribers. Thus we conducted this study, aiming to determine the frequency of S. aureus and coagulase-negative staphylococci (CNS) strains isolation in different types of biologic samples, and to investigate the link between methicillin resistance and ciprofloxacin resistance. Methods: We conducted this study from January 2006 through Jun 2010. The Microbiology Laboratory Service collected and analyzed samples for diagnostic purpose from inpatients and outpatients consulted in the hospital. We collected and analyzed de-identified data on these patients to form laboratory records. Bacteriological analyses in which ciprofloxacin have not been tested were excluded. Results: Over the 1108 staphylococal strains isolated from various biological samples processed, 751 were Staphylococcus aureus and 357 were coagulase-negative staphylococci. The strains sensitivity profile is for all isolates. The majority of germs were S. aureus and 20% of them were ciprofloxacin-resistant. The probability of a patient who has an infection caused by S. aureus increases his stay in high-risk settings such as intensive care unit, surgical intervention, extended hospitalization, use of a catheter. The use of broad-spectrum antibiotics increases the risk of multidrug-resistant strains. Conclusion: This study highlights the recurring issue of over consumption of antibiotics in nowadays medical treatments. There is a need to raise awareness about the rational use of antibiotics in general and fluoroquinolones particularly.

Keywords

Staphylococcus, Ciprofloxacin, Methicillin, Resistance

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Mylotte, J.M., McDermott, C. and Spooner, J.A. (1987) Prospective Study of 114 Consecutive Episodes of Staphylococcus aureus Bacteremia. Clinical Infectious Diseases, 9, 891-907. http://dx.doi.org/10.1093/clinids/9.5.891
[2]	Boyce, J.M., White, R.L. and Spruill, E.Y. (1983) Impact of Methicillin-Resistant Staphylococcus aureus on the Incidence of Nosocomial Staphylococcal Infections. The Journal of Infectious Diseases, 148, 763. http://dx.doi.org/10.1093/infdis/148.4.763
[3]	Dagnra, A.Y., Hounkpati, A. and Prince-David, M. (2001) Fort Pourcentage de souches de Staphylococcus aureus résistantes à la méticilline au CHU de Lomé (Togo). Médecineet et Maladies Infectieuses, 31, 14-18.
[4]	Wolfson, J.S. and Hooper, D.C. (1989) Fluoroquinolone Antimicrobial Agents. Clinical Microbiology Reviews, 2, 378-424.
[5]	National Committee for Clinical Laboratory Standards (2003) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically: Approved Standard M7-A6. NCCLS, Wayne, Pennsylvania, USA.
[6]	Datta, P., et al. (2011) Evaluation of Various Methods for the Detection of Methicillin-Resistant Staphylococcus aureus Strains and Susceptibility Patterns. Journal of Medical Microbiology, 60, 1613-1616. http://dx.doi.org/10.1099/jmm.0.032219-0
[7]	Bonnet, R., et al. (2011) Comité de l’antibiogramme de la Société Française de Microbiologie: Recommandations 2011. Société Française de Microbiologie, Paris.
[8]	Jarvis, W.R., Jarvis, A.A. and Chinn, R.Y. (2012) National Prevalence of Methicillin-Resistant Staphylococcus aureus in Inpatients at United States Health Care Facilities, 2010. American Journal of Infection Control, 40, 194-200. http://dx.doi.org/10.1016/j.ajic.2012.02.001
[9]	Simor, A.E., et al. (2001) The Evolution of Methicillin-Resistant Staphylococcus aureus in Canadian Hospitals: 5 Years of National Surveillance. Canadian Medical Association Journal, 165, 21-26.
[10]	Kobayashi, H. (2005) National Hospital Infection Surveillance on Methicillin-Resistant Staphylococcus aureus. Journal of Hospital Infection, 60, 172-175. http://dx.doi.org/10.1016/j.jhin.2004.12.010
[11]	Akoua-Koffi, C., et al. (2004) La méticillino-résistance de Staphylococcus aureus isolés à Abidjan (1998-2001): Un nouveau problème en milieu hospitalier. Medecineet et Maladies Infectieuses, 34, 132-136. http://dx.doi.org/10.1016/j.medmal.2003.12.001