Effects of mRNA, Protein Expression and Activity for Myocardial SOD2 by a Single Bout of or Long-Term Strenuous Endurance Exercise in Rats


Objective: To explore the effects of myocardial SOD2 by strenuous endurance exercise. Methods: 27 grown male SD rats were randomly divided into control group (C), a single bout of strenuous endurance exercise group (E1) and seventh-week strenuous endurance exercise group (E2). Real-time PCR was used to observe the changes of mRNA expression for myocardial SOD2. Western bolt was used to observe the changes of SOD2 protein expression. In addition, SOD2, T-SOD and SOD1 activity changes were observed. Results: Myocardial SOD2 expression level at mRNA and protein of Group E1, E2 was significantly higher than that in group C, and SOD2 and T-SOD activity in group E2 were significantly higher than those in group C. Those changes were more obvious in group E2. Conclusions: Strenuous endurance exercise can improve level of myocardial SOD2 expression at mRNA and protein, and enhance the activity for SOD2, thus increasing the activity for T-SOD. Effect of long-term strenuous endurance exercise was better than a single bout of one.

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Xu, S. , Liu, W. and Li, M. (2014) Effects of mRNA, Protein Expression and Activity for Myocardial SOD2 by a Single Bout of or Long-Term Strenuous Endurance Exercise in Rats. Chinese Medicine, 5, 251-258. doi: 10.4236/cm.2014.54030.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Ascensao, A., Ferrira, R. and Magalhaes, J. (2007) Exercise-Induced Cardioprotection-Biochemical, Morpho-Logical and Functional Evidence in Whole Tissue and Isolated Mitochondria. International Journal of Cardiology, 117, 16-30.
[2] Brown, D.A., Chicco, A.J., Jew, K.N., Johnson, M.S., Lynch, J.M., Watson, P.A. and Moore, R.L. (2005) Cardioprotection Afforded by Chronic Exercise Is Mediated by the Sarcolemmal, and Not the Mitochondrial, Isoform of the KATP Channel in the Rat. The Journal of Physiology, 569, 913-924.
[3] Budiono, B.P., Hoe, L.E.S., Peart, J.N., Sabapathy, S., Ashton, K.J., Haseler, L.J. and Headrick, J.P. (2012) Voluntary Running in Mice Beneficially Modulates Myocardial Ischemic Tolerance, Signaling Kinases, and Gene Expression Patterns. American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 302, R1091-R1100.
[4] French, J.P., Quindry, J.C., Falk, D.J., Staib, J.L., Lee, Y., Wang, K.K.W. and Powers, S.K. (2006) Ischemia-Reperfusion-Induced Calpain Activation and SERCA2a Degradation Are Attenuated by Exercise Training and Calpain Inhibition. American Journal of Physiology. Heart and Circulatory Physiology, 290, H128-H136.
[5] Powers, S.K., Sollanek, K.J., Wiggs, M.P., Demirel, H.A. and Smuder, A.J. (2014) Exercise-Induced Improvements in Myocardial Antioxidant Capacity: The Antioxidant Players and Cardioprotection. Free Radical Research, 48, 43-51.
[6] Bedford, T.G., Tipton, C.M., Wilson, N.C., Oppliger, R.A. and Gisolfi, C.V. (1979) Maximum Oxygen Consumption of Rats and Its Changes with Various Experimental Procedures. Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology, 47, 1278-1283.
[7] Pfaffl, M.W. (2001) A New Mathematical Model for Relative Quantification in Real-Time PT-PCR. Nucleic Acids Research, 29, e45.
[8] Peng, F.L., Lu, Y.L., Zhang, L., Chen, D.Q. and Deng, S.X. (2009) The Cardioprotective Effect of Intermittent Exercises on Ischemia-Reperfusion Rats’ Myocardium. Chinese Journal of Rehabilitation Medicine, 24, 910-913.
[9] Peng, F.L., Guo, Y.J. and Zhang, L. (2010) The Effects of Interval Training and Acute Interval Exercise on IschemiaReperfusion Rats’ ECG and Serum Myocardial Enzymes. China Sport Science and Technology, 46, 102-105.
[10] Xu, S.M., Liu, T.B. and Su, Q.S. (2011) Study on Heavy Load Training-Induced Cardiac Contractility Changes of Rats, and the Relationship between This Changes and the Levels of Cardiac Free Radical, Calcium Ions. China Sport Science, 31, 73-78.
[11] Li, S.C., Duan, Y.M. and Su, Q.S. (2012) The Establishment of Exercise-Induced Myocardial Stunning Model of Rat with Repeated Exhaustive Exercise on Treadmills. China Sport Science, 32, 49-54.
[12] Xu, S.M, Zhou, Y. and Wang, J. (2014) Abnormal ECG Changes and Energy Metabolic Disturbance after an Exhaustive Exercises in Rats. Journal of Tianjin University of Sport, 29, 132-135.
[13] Su, Q.S. (2004) Effect on the Structure of Heart during Re-Oxygen Process. Journal of Chengdu Physical Education Institute, 30, 61-64.
[14] Yamashita, N., Hoshida, S., Otsu, K., Asahi, M., Kuzuya, T. and Hori, M. (1999) Exercise Provides Direct Biphasic Cardioprotection via Manganese Superoxide Dismutase Activation. The Journal of Experimental Medicine, 189, 16991706.
[15] Hou, Y.Y, Ma, Z.Y. and Fan, X.Z. (2010) Protective Effect of Short-Term Exercise on Rat Ischemic/Reperfused Myocardium and Its Mechanism. Suzhou University Journal of Medical Science, 30, 50-53.
[16] Wenz, T., Diaz, F., Hernandez, D. and Moraes, C.T. (2009) Endurance Exercise Is Protective for Mice with Mitochondrial Myopathy. Journal of Applied Physiology, 106, 1712-1719.
[17] Smuder, A.J., Min, K., Hudson, M.B., Kavazis, A.N., Kwon, O., Nelson, W.B. and Powers, S.K. (2012) Endurance Exercise Attenuates Ventilator-Induced Diaphragm Dysfunction. Journal of Applied Physiology, 112, 501-510.

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