Antimicrobial Activity of Traditional Chinese Medicines on Common Oral Bacteria
Michelle K. Z. Yuen, Ricky W. K. Wong, Urban Hägg, Lakshman Samaranayake
DOI: 10.4236/cm.2011.22007   PDF   HTML     6,391 Downloads   13,139 Views   Citations


Objective: To evaluate twenty Traditional Chinese Medicines (TCM) against four oral bacteria. Methods: Twenty TCM were tested for sensitivity against Streptococcus mitis, Streptococcus sanguis, Streptococcus mutans and Porphyromonas gingivalis. Aliquots of suspension of each bacterial species were inoculated on a horse blood agar (HBA) plate, 6 mm diameter paper disks was soaked in different drug suspensions were placed concentrically on a HBA plate. Disks soaked in 0.2% w/v chlorhexidine were used as positive controls. These HBA plates were incubated for 48 hours anaerobically and the diameters of growth inhibition of three different areas were measured using a calibrated computer software and the mean diameter obtained for each bacteria. Broth microdilution assay was used to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The experiment was repeated on three separate occasions. Results: The TCMs that consistently against Porphyromonas gingivalis, included Folium artemisiae argyi, Fructus crataegi, Rhizoma dryopteris crassirhizomae, Flos magnoliae, Rhizoma polygoni cuspidati, Radix scrophulariae ningpoensis, Galla chinensis, Radix scutellariae baicalensis and Rhizoma coptidis; against Streptococcus mutans included Fructus crataegi, Galla chinensis and Rhizoma copitidis; against Streptococcus mitis and Streptococcus sanguis included Galla chinensis and Rhizoma copitidis. Conclusion: Rhizoma copitidis and Galla chinensis had inhibitory effects on Streptococcus mitis, Streptococcus sanguis, Streptococcus mutans and Porphyromonas gingivalis in vitro.

Share and Cite:

M. Yuen, R. Wong, U. Hägg and L. Samaranayake, "Antimicrobial Activity of Traditional Chinese Medicines on Common Oral Bacteria," Chinese Medicine, Vol. 2 No. 2, 2011, pp. 37-42. doi: 10.4236/cm.2011.22007.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] L. P. Samaranayake, “Essential Microbiology for dentistry,” Churchill Livingstone, Churchill, 2006.
[2] T. M. Auschill and N. Hein, “Effect of Two Antimicrobial Agents on early in Situ Biofilm Formation,” Journal of Clinical Periodontology, Vol. 32, No. 2, February 2005, pp. 147-152. doi:10.1111/j.1600-051X.2005.00650.x
[3] D. M. Eisenberg, “Trends in Alternative Medicine Use in the United States,” Journal of the American Medical Association, Vol. 280, No. 18, 1998, pp. 1569-1575. doi:10.1001/jama.280.18.1569
[4] L. Wang, G. B. Zhou, P. Liu, J. H. Song, Y. Liang, X. J. Uan, F. Xu, B. S. Wang, J. H. Mao, Z. X. Shen, S. J. Chen and Z. Chen, “Dissection of Mechanisms of Chinese Medicinal Formula Realgar-Indigo Naturalis as an Effective Treatment for Promyelocytic Leukemic,” Proceedings of the National Academy of Sciences, Vol. 105, No. 12, March 2008, pp. 4826-4831. doi:10.1073/pnas.0712365105
[5] J. P. Hu, N. Takahashi and T. Yamada, “Coptidis Rhizoma Inhibits Growth and Proteases of Oral Bacteria,” Oral Disease, Vol. 6, No. 5, September 2000, pp. 297-302. doi:10.1111/j.1601-0825.2000.tb00142.x
[6] M. Levison, “Antibacterial Therapy,” In: Vitro Testing, Pharmaco-Dynamics, Pharmacology, New Agents, Infectious Disease Clinics of North America, Vol. 9, 1995, pp. 483-495.
[7] H. H. Yu, K. J. Kim, J. D. Cha, H. K. Kim, Y. E. Lee, N. Y. Choi and Y. O. You, “Antimicrobial Activity of Berberine Alone and in Combination with Ampicillin or Oxacillin against Methicillin-Resistant Staphylococcus Aureus,” Journal of Medical Food, Vol. 8, No. 4, December 2005, pp. 454-461. doi:10.1089/jmf.2005.8.454
[8] E. A. Bae, M. J. Han, N. J. Kim and D. H. Kim, “Anti-Helicobacter Pylori Activity of Herbal Medicines,” Biological Pharmarcy Bulletin, Vol. 21, No. 9, September 1998, pp. 990-992.
[9] S. Higaki, M. Nakamura, M. Morohashi, Y. Hasegawa and T. Yamagishi, “Anti-Lipase Activity of Kampo Formulations, Coptidis Rhizoma and Its Alkaloids against Propionibacterium Acnes,” Journal of Dermatology, Vol. 23, No. 5, 1996, pp. 310-314.
[10] R. W. K. Wong, U. Hagg, L Samaranayake, M. K. Z. Yuen, C. J. Seneviratne and R. Kao, “Antimicrobial Activity of Chinese Medicine Herbs against Common Bacteria in Oral Biofilm: A Pilot Study,” International Journal of Oral and Maxillofacial Surgery, Vol. 39, No. 6, 2010, pp. 599-605. doi:10.1016/j.ijom.2010.02.024

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.