Green Tea (Camellia sinensis): Hypocholesterolemic Effects in Humans and Anti-Inflammatory Effects in Animals


In its essence, tea is an infusion of Camellia sinensis leaves and it is the most widely consumed beverage in the world, aside water. Animal and epidemiological studies have associated green tea consumption with several health benefits, which include hypocholesterolemic effect and anti-in-flammatory activity. In this study catechins levels of green tea and its effect on the lipid profile of humans were evaluated as well as the protective effects against H2O2-mediated damage in human fibroblasts and anti-inflammatory activity of ()-epigallocatechingallate (EGCG). The daily consumption of 1200 mL of green tea for 30 days by 15 human volunteers promoted the decrease of cholesterol and LDL levels after 15 days, but this effect was not persistent after 30 days. No changes were observed in lipid profile after the consumption of green tea capsules. EGCG, a major green tea catechin, demonstrated an anti-inflammatory action in rats and an antioxidant effect in cultured human fibroblasts.

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Reto, M. , Almeida, C. , Rocha, J. , Sepodes, B. and Figueira, M. (2014) Green Tea (Camellia sinensis): Hypocholesterolemic Effects in Humans and Anti-Inflammatory Effects in Animals. Food and Nutrition Sciences, 5, 2185-2194. doi: 10.4236/fns.2014.522231.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] MacKay, D.L. and Blumberg, J.B. (2000) The Role of Tea in Human Health: An Update. Journal of the American College of Nutrition, 21, 1-13.
[2] Reto, M., Figueira, M.E., Filipe, H.M. and Almeida, C.M.M. (2007) Chemical Composition of Green Tea (Camellia sinensis) Infusions Commercialized in Portugal. Plant Foods for Human Nutrition, 62, 139-144.
[3] Reto, M., Figueira, M.E., Filipe, H.M. and Almeida, C.M.M. (2007) Analysis of Vitamin K in Green Tea Leafs and Infusions by SPME-GC-FID. Food Chemistry, 100, 405-411.
[4] Reto, M., Figueira, M.E., Filipe, H.M. and Almeida, C.M.M. (2008) Teor de Fluoretos em Infus?es de Chá Verde (Camellia sinensis). Química Nova, 31, 317-320.
[5] WHO (2007) Cardiovascular Diseases. Fact Sheet, No. 317.
[6] Frostegard, J. (2013) Immunity, Atherosclerosis and Cardiovascular Disease. BMC Medicine, 11, 117.
[7] Orekhov, A.N. (2014) Direct Anti-Atherosclerotic Therapy; Development of Natural Anti-Atherosclerotic Drugs Preventing Cellular Cholesterol Retention. Current Pharmaceutical Design, 19, 5909-5928.
[8] Bursill, C.A., Abbey, M. and Roach, P.D. (2007) A Green Tea Extract Lowers Plasma Cholesterol by Inhibiting Cholesterol Synthesis and Upregulating the LDL Receptor in the Cholesterol-Fed Rabbit. Atherosclerosis, 193, 86-93.
[9] Paoletti, R., Gotto A.M., DPhil, M.D. and Hajjar, D. (2004) Inflammation in Atherosclerosis and Implications for Therapy. Circulation, 109, 20-26.
[10] Sherer, Y. and Shoenfeld, Y. (2006) Mechanisms of Disease: Atherosclerosis in Autoimmune Diseases. Nature Clinical Practice Rheumatology, 2, 99-106.
[11] Kolluru, G.K., Bir, S.C. and Kevil, C.G. (2012) Endothelial Dysfunction and Diabetes: Effects on Angiogenesis, Vascular Remodeling, and Wound Healing. International Journal of Vascular Medicine, 2012, Article ID: 918267, 30 p.
[12] Peters, U., Poole, C. and Arab, L. (2001) Does Tea Affect Cardiovascular Disease? A Meta-Analysis. American Journal of Epidemiology, 154, 495-503.
[13] Bacquer, D.D., Clays, E., Delanghe, J. and Backer, G.D. (2006) Epidemiological Evidence for an Association between Habitual Tea Consumption and Markers of Chronic Inflammation. Atherosclerosis, 189, 428-435.
[14] Zheng, X.X., Xu, Y.L., Li, S.H., Liu, X.X., Hui, R. and Huang, X.H. (2011) Green Tea Intake Lowers Fasting Serum Total and LDL Cholesterol in Adults: A Meta-Analysis of 14 Randomized Controlled Trials. American Journal of Clinical Nutrition, 94, 601-610.
[15] Davies, M.J., Judd, J.T., Baer, D.J., Clevidence, B.A., Paul, D.R., Edwards, A.J., Wiseman, S.A., Muesing, R.A. and Chen, S.C. (2003) Black Tea Consumption Reduces Total and LDL Cholesterol in Mildly Hypercholesterolemic Adults. Journal of Nutrition, 133, 3298S-3302S.
[16] Das, M., Sur, P., Gomes, A., Vedasiromoni, J.R. and Ganguly, D.K. (2002) Inhibition of Tumour Growth and Inflam- mation by Consumption of Tea. Phytotherapy Research, 16, 40-44.
[17] Varilek, G.W., Yang, F., Lee, E.Y., de Villiers, J.S., Zhong, J., Oz, H.S., Westberry, K.F. and McClain, C.J. (2001) Green Tea Polyphenol Extract Attenuates Inflammation Interleukin-2-Deficient Mice, a Model of Autoimmunity. Journal of Nutrition, 131, 2034-2039.
[18] Yang, F., de Villiers, J.S., McClain, C.J. and Varilek, G.W. (1998) Green Tea Polyphenols Block Endotoxin-Induced Tumor Necrosis Factor-Production and Lethality in a Murine Model. Journal of Nutrition, 128, 2334-2340.
[19] Chan, M.M., Fong, D., Ho, C.T. and Huang, H.I. (1997) Inhibition of Inducible Nitric Oxide Synthase Gene Expression and Enzyme Activity by Epigallocatechin Gallate, a Natural Product from Green Tea. Biochemical Pharmacology, 54, 1281-1286.
[20] Lin, Y.L. and Lin, J.K. (1997) (–)-Epigallocatechin-3-gallate Induction of Nitric Oxide Synthase by Down-Regulating Lipopolysacharide-Induced Activity of Transcription Factor Nuclear Factor-κB. Molecular Pharmacology, 52, 465- 472.
[21] Lin, Y.L., Tsai, S.H., Lin-Shiau, S.Y., Ho, C.T. and Lin, J.K. (1999) Theaflavin-3,3’-digallate from Black Tea Blocks the Nitric Oxide Synthase by Down-Regulating the Activation of NF-κB in Macrophages. European Journal of Pharmacology, 367, 379-388.
[22] Lin, Y.S., Wu, S.S. and Lin, J.K. (2003) Determination of Tea Polyphenols and Caffeine in Tea Flowers (Camellia sinensis) and Their Hydroxyl Radical Scavenging and Nitric Oxide Suppressing Effects. Journal of Agricultural and Food Chemistry, 51, 975-980.
[23] Frei, B. and Higdon, J.V. (2003) Antioxidant Activity of Tea Polyphenols in Vivo: Evidence from Animal Studies. Journal of Nutrition, 133, 3275S-3284S.
[24] Vogel, H.G. (2002) Drug Discovery and Evaluation: Pharmacological Assays. Springer-Verlag Berlin and Heidelberg GmbH & Co., Berlin, Heidelberg, 751-772.
[25] Bignotto, L., Rocha, J., Sepodes, B., Eduardo-Figueira, M., Pinto, R., Chaud, M., et al. (2009) Anti-Inflammatory Effect of Lycopene on Carrageenan-Induced Paw Oedema and Hepatic Ischaemia-Reperfusion in the Rat. British Journal of Nutrition, 102, 126-133.
[26] Cole, S.P. (1986) Rapid Chemosensitivity Testing of Human Lung Tumor Cells Using the MTT Assay. Cancer Chemotherapy and Pharmacology, 17, 259-263.
[27] Sousa, J.C. and Pinto, D.S. (2003) Valores Laboratoriais do estudo lipídico e risco vascular. Revista Faculdade de Medicina de Lisboa Série III, 8, 75-81.
[28] Kono, S., Shinchi, K., Ikeda, N., Yanai, F. and Imanishi, K. (1992) Green Tea Consumption and Serum Lipid Profiles: A Cross-Sectional Study in Northern Kyushu, Japan. Preventive Medicine, 21, 526-531.
[29] Imai, K. and Nakachi, K. (1995) Cross Sectional Study of Effects of Drinking Green Tea on Cardiovascular and Liver Diseases. British Medical Journal, 310, 693-696.
[30] Tokunaga, S., White, I.R., Frost, C., Tanaka, K., Kono, S., Tokudome, S., Akamatsu, T., Moriyama, T. and Zakouji, H.A. (2002) Green Tea Consumption and Serum Lipids and Lipoproteins in a Population of Healthy Workers in Japan. Annals of Epidemiology, 12, 157-165.
[31] Maron, D.J., Lu, G.P., Ca, N.S., Wu, Z.G., Li, Y.H., Chen, H., Zhu, J.Q., Jin, X.J., Wouters, B.C. and Zhao, J. (2003) Cholesterol-Lowering Effect of a Theaflavin-Enriched Green Tea Extract: A Randomized Controlled Trial. JAMA Internal Medicine, 163, 1448-1453.
[32] van het Hof, K.H., de Boer, H.S., Wiseman, S.A., Lien, N., Westrate, J.A. and Tijburg, L.B. (1997) Consumption of Green or Black Tea Does Not Increase Resistance of Low-Density Lipoprotein to Oxidation in Humans. American Journal of Clinical Nutrition, 66, 1125-1132.
[33] Princen, H.M., van Duyvenvoorde, W., Buytenhek, R., Blonk, C., Tijburg, L.B., Langius, J.A., Meinders, A.E. and Pijl, H. (1998) No Effect of Consumption of Green and Black Tea on Plasma Lipid and Antioxidant Levels and on LDL Oxidation in Smokers. Arteriosclerosis, Thrombosis, and Vascular Biology, 18, 833-841.
[34] Yang, T.T. and Koo, M.W. (1999) Chinese Green Tea Lowers Cholesterol Level trough an Increase in Fecal Lipid Excretion. Life Sciences, 66, 411-423.
[35] Bursill, C., Roach, P.D., Bottema, C.D. and Pal, S. (2001) Green Tea Upregulates the Low-Density Lipoprotein Receptor through the Sterol-Regulated Element Binding Protein in HepG2 Liver Cells. Journal of Agricultural and Food Chemistry, 49, 5639-5645.
[36] Raederstorff, D.G., Schlachter, M.F., Elste, V. and Weber, P. (2003) Effect of EGCG on Lipid Absorption and Plasma Lipid Levels in Rats. Journal of Nutritional Biochemistry, 14, 326-332.
[37] Koo, S.I. and Noh, S.K. (2007) Green Tea as Inhibitor of the Intestinal Absorption of Lipids: Potential Mechanism for Its Lipid-Lowering Effect. Journal of Nutritional Biochemistry, 18, 179-183.
[38] Donà, M., Dell’Aica, I., Calabrese, F., Benelli, R., Morini, M., Albini, A. and Garbisa, S. (2003) Neutrophil Restraint by Green Tea: Inhibition of Inflammation, Associated Angiogenesis, and Pulmonary Fibrosis. Journal of Immunology, 170, 4335-4341.
[39] Hong, J., Smith, T.J., Ho, C.T., August, D.A. and Yang, C.S. (2001) Effects of Purified Green and Black Tea Polyphenols on Cyclooxygenase- and Lipoxygenase-Dependent Metabolism of Arachidonic Acid in Human Colon Mucosa and Colon Tumor Tissues. Biochemical Pharmacology, 62, 1175-1183.
[40] August, D.A., Landau, J., Caputo, D., Hong, J., Lee, M.J. and Yang, C.S. (1999) Ingestion of Green Tea Rapidly Decreases Prostaglandin E2 Levels in Rectal Mucosa in Humans. Cancer Epidemiology, Biomarkers & Prevention, 8, 709-713.
[41] Paquay, J.B., Haenen, G.R., Stender, G., Wiseman, S.A., Tijburg, L.B. and Bast, A. (2000) Protection against Nitric Oxide Toxicity by Tea. Journal of Agricultural and Food Chemistry, 48, 5768-5772.
[42] Aneja, R., Hake, P.W., Burroughs, T.J., Denenberg, A.G., Wong, H.R. and Zingarelli, B. (2004) Epigallocatechin, a Green Tea Polyphenol, Attenuates Myocardial Ischemia Reperfusion Injury in Rats. Molecular Medicine, 10, 55-62.
[43] Cao, H., Kelly, M.A., Kari, F., Dawson, H.D., Urban, J.F., Coves, S., Roussel, A.M. and Anderson, R.A. (2007) Green Tea Increases Anti-Inflammatory Tristetraprolin and Decreases Pro-Inflammatory Tumor Necrosis Factor mRNA Levels in Rats. Journal of Inflammation, 4, 1-12.
[44] Saffari, Y. and Sadrzadeh, S.M. (2004) Green Tea Metabolite EGCG Protects Membranes against Oxidative Damage in Vitro. Life Sciences, 74, 1513-1518.
[45] Kanadzua, M., Lua, Y. and Morimoto, K. (2006) Dual Function of (–)-epigallocatechingallate (EGCG) in Healthy Human Lymphocytes. Cancer Letters, 241, 250-255.
[46] Muzolf, M., Szymusiak, H., Gliszczyñska-Swiglo, A., Rietjens, I.M. and Tyrakowska, B. (2008) pH-Dependent Radical Scavenging Capacity of Green Tea Catechins. Journal of Agricultural and Food Chemistry, 56, 816-823.

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