Dps Is a Stationary Phase-Specific Protein of Escherichia coli Nucleoid

DOI: 10.4236/aim.2014.415120   PDF   HTML   XML   2,978 Downloads   3,742 Views   Citations


Bacterial genomic DNA is highly organized into one or few compacted bodies known as nucleoid, which is composed of DNA, RNA and several DNA-binding proteins. These DNA-binding proteins require essential alterations in their expression during stationary phase of growth in order to re-spond to stressful environmental conditions. Dps (DNA-binding protein from starved cells) is one of such DNA-binding proteins, which accumulates most when E. coli cells reach to the stationary phase. Here, we have characterized Dps protein under various growth phases. Immunofluorescent microscopic observation reveals that Dps plays a key role in final round of genome compaction during the stationary phase. Similar results are also obtained by Western immunoblot analysis, after quantification of Dps protein from the exponential phase and early stationary phase nucleoid bound fractions, separated by sucrose density gradient centrifugation. Our results support the conclusion that Dps occupies more than half of the stationary phase nucleoid in E. coli.

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Talukder, A. and Ishihama, A. (2014) Dps Is a Stationary Phase-Specific Protein of Escherichia coli Nucleoid. Advances in Microbiology, 4, 1095-1104. doi: 10.4236/aim.2014.415120.

Conflicts of Interest

The authors declare no conflicts of interest.


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