Isolated Soy Protein-Based Diet Ameliorates Glycemia and Antioxidants Enzyme Activities in Streptozotocin-Induced Diabetes
Roberta Hack Mendes1,2, Martine Kienzle Hagen1,3, Jaqueline Barp1, Erna Vogt de Jong4, Júlia Dubois Moreira5, Álvaro Reischak-Oliveira6, Maria Cláudia Irigoyen1,7, Adriane Belló-Klein1
1Physiology Departament, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
2Institute of Nutrition Josué de Castro, Rio de Janeiro Federal University, Rio de Janeiro, Brazil.
3Department of Internal Medicine, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
4Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
5Department of Nutrition, Health Sciences Centre, Federal University of Santa Catarina, Florianópolis, Brazil.
6School of Physical Education, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
7Hypertension Unit, Heart Institute, Federal University of São Paulo, São Paulo, Brazil.
DOI: 10.4236/fns.2014.521221   PDF   HTML   XML   2,768 Downloads   3,334 Views   Citations


The objective of this study was to evaluate the changes induced by isolated soy protein (ISP)-based diet on glycemia and oxidative stress biomarkers in diabetic rats. Fifteen male Wistar rats (35 ± 4 g, aged 21 days) were assigned to three groups: Casein (C group), which received casein-based diet during experimental protocol; Diabetic treated with Casein (D + C group) that received casein-based diet before and after diabetes induction; Diabetic treated with ISP (D + S group) that received casein-based diet before diabetes induction and after received ISP-based diet for the experimental protocol. Diabetes was induced by a single dose of streptozotocin (50 mg/kg body weight i.v.). After three weeks of dietary treatment, total nitrates, lipid peroxidation, antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST) were measured in heart homogenates. ISP-based diet promoted an improvement in the glycemic levels of diabetic rats compared with casein-based diet (362 ± 25 vs 461 ± 30 mg/dL). CAT activity demonstrated a significant decrease in D + C and D + S groups. D + S group presented a significant increase in SOD and GST activities. Lipid peroxidation was not different among experimental groups. The overall results suggested the potential benefits of ISP-based diet consumption to improve the life quality of diabetic patients.

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Mendes, R. , Hagen, M. , Barp, J. , de Jong, E. , Moreira, J. , Reischak-Oliveira, Á. , Irigoyen, M. and Belló-Klein, A. (2014) Isolated Soy Protein-Based Diet Ameliorates Glycemia and Antioxidants Enzyme Activities in Streptozotocin-Induced Diabetes. Food and Nutrition Sciences, 5, 2089-2096. doi: 10.4236/fns.2014.521221.

Conflicts of Interest

The authors declare no conflicts of interest.


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